RP-HPLC–UV method for the quantification of propranolol in rat's serum and krebs buffer using one-step protein precipitation

Krebs buffer is considered one of the most used physiological buffers in biomedical research. In the current work, a rapid reversed-phase high-performance liquid chromatographic (RP-HPLC) method with ultraviolet (UV) detection at 214 nm was developed and validated according to European Medicines Eva...

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Veröffentlicht in:	Acta Chromatographica 2018-09, Vol.30 (3), p.147-152
Hauptverfasser:	Al Shaker, Hanadi A, Qinna, Nidal A, Al Hroub, Hamza, Al Omari, Mahmoud M. H, Badwan, Adnan A
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetonitrile Biomedical materials Blood serum Buffers Chemical properties Chemical research Dilution Elution Flow rates Flow velocity High performance liquid chromatography In vivo methods and tests Liquid chromatography Methods Physiological aspects Propranolol Proteins Rats Sildenafil Stability analysis Stability tests Storage conditions
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