Epithelial-to-mesenchymal transition and some parameters of extracellular matrix remodeling in chronic rhinosinusitis with nasal polyps
Objective. The purpose of our study was to evaluate vimentin expression in nasal polyp tissue, concentrations of monocyte chemoattractant protein-1 (MCP-1) and matrix metalloproteinase-9 (MMP-9) in blood serum of patients with chronic rhinosinusitis with nasal polyps (CRSwNP).Materials and methods....
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| Veröffentlicht in: | Human & veterinary medicine 2018-09, Vol.10 (3), p.128-132 |
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| creator | Onishchenko, Anatolii I Lupyr, Andrii V Tkachenko, Anton S Gorbach, Tatyana V Nakonechna, Oksana A Gubina-Vakulyck, Galina I |
| description | Objective. The purpose of our study was to evaluate vimentin expression in nasal polyp tissue, concentrations of monocyte chemoattractant protein-1 (MCP-1) and matrix metalloproteinase-9 (MMP-9) in blood serum of patients with chronic rhinosinusitis with nasal polyps (CRSwNP).Materials and methods. Levels of MMP-9 and MCP-1 were determined in blood serum of twenty patients with CRSwNP using the commercially produced ELISA kits. Immunohistochemical vimentin staining using specimens of nasal polyp tissue was performed. The results were compared to the control group consisted of twenty relatively healthy subjects.Results. Vimentin was found to be strongly expressed in both the lamina propria and epithelial cells in nasal polyp tissue of patients with CRSwNP. Blood serum concentrations of MMP-9 and MCP-1 were higher in CRSwNP compared to the control individuals. MCP-1/MMP-9 ratio was higher as well. Conclusion. The development of nasal polyps is accompanied by an increase in the amount of vimentin-positive cells in the epithelial layer, which indicates the activation of endothelial-to-mesenchymal transition (EMT). CRSwNP is associated with the imbalance between profibrotic and antifibrotic factors. |
| format | Article |
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The purpose of our study was to evaluate vimentin expression in nasal polyp tissue, concentrations of monocyte chemoattractant protein-1 (MCP-1) and matrix metalloproteinase-9 (MMP-9) in blood serum of patients with chronic rhinosinusitis with nasal polyps (CRSwNP).Materials and methods. Levels of MMP-9 and MCP-1 were determined in blood serum of twenty patients with CRSwNP using the commercially produced ELISA kits. Immunohistochemical vimentin staining using specimens of nasal polyp tissue was performed. The results were compared to the control group consisted of twenty relatively healthy subjects.Results. Vimentin was found to be strongly expressed in both the lamina propria and epithelial cells in nasal polyp tissue of patients with CRSwNP. Blood serum concentrations of MMP-9 and MCP-1 were higher in CRSwNP compared to the control individuals. MCP-1/MMP-9 ratio was higher as well. Conclusion. The development of nasal polyps is accompanied by an increase in the amount of vimentin-positive cells in the epithelial layer, which indicates the activation of endothelial-to-mesenchymal transition (EMT). CRSwNP is associated with the imbalance between profibrotic and antifibrotic factors.</description><identifier>ISSN: 2066-7655</identifier><identifier>EISSN: 2066-7663</identifier><language>eng</language><publisher>Cluj-Napoca: Bioflux SRL</publisher><subject>Age ; Blood ; Breast cancer ; Cancer therapies ; Cell adhesion & migration ; Enzyme-linked immunosorbent assay ; Epithelial cells ; Extracellular matrix ; Gelatinase B ; Hypoxia ; Inflammation ; Lamina propria ; Matrix metalloproteinase ; Mesenchyme ; Metalloproteinase ; Metastasis ; Monocyte chemoattractant protein ; Monocyte chemoattractant protein 1 ; Monocytes ; Motility ; Polyps ; Proteins ; Rhinosinusitis ; Vimentin</subject><ispartof>Human & veterinary medicine, 2018-09, Vol.10 (3), p.128-132</ispartof><rights>Copyright Bioflux SRL Sep 2018</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Onishchenko, Anatolii I</creatorcontrib><creatorcontrib>Lupyr, Andrii V</creatorcontrib><creatorcontrib>Tkachenko, Anton S</creatorcontrib><creatorcontrib>Gorbach, Tatyana V</creatorcontrib><creatorcontrib>Nakonechna, Oksana A</creatorcontrib><creatorcontrib>Gubina-Vakulyck, Galina I</creatorcontrib><title>Epithelial-to-mesenchymal transition and some parameters of extracellular matrix remodeling in chronic rhinosinusitis with nasal polyps</title><title>Human & veterinary medicine</title><description>Objective. The purpose of our study was to evaluate vimentin expression in nasal polyp tissue, concentrations of monocyte chemoattractant protein-1 (MCP-1) and matrix metalloproteinase-9 (MMP-9) in blood serum of patients with chronic rhinosinusitis with nasal polyps (CRSwNP).Materials and methods. Levels of MMP-9 and MCP-1 were determined in blood serum of twenty patients with CRSwNP using the commercially produced ELISA kits. Immunohistochemical vimentin staining using specimens of nasal polyp tissue was performed. The results were compared to the control group consisted of twenty relatively healthy subjects.Results. Vimentin was found to be strongly expressed in both the lamina propria and epithelial cells in nasal polyp tissue of patients with CRSwNP. Blood serum concentrations of MMP-9 and MCP-1 were higher in CRSwNP compared to the control individuals. MCP-1/MMP-9 ratio was higher as well. Conclusion. The development of nasal polyps is accompanied by an increase in the amount of vimentin-positive cells in the epithelial layer, which indicates the activation of endothelial-to-mesenchymal transition (EMT). CRSwNP is associated with the imbalance between profibrotic and antifibrotic factors.</description><subject>Age</subject><subject>Blood</subject><subject>Breast cancer</subject><subject>Cancer therapies</subject><subject>Cell adhesion & migration</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Epithelial cells</subject><subject>Extracellular matrix</subject><subject>Gelatinase B</subject><subject>Hypoxia</subject><subject>Inflammation</subject><subject>Lamina propria</subject><subject>Matrix metalloproteinase</subject><subject>Mesenchyme</subject><subject>Metalloproteinase</subject><subject>Metastasis</subject><subject>Monocyte chemoattractant protein</subject><subject>Monocyte chemoattractant protein 1</subject><subject>Monocytes</subject><subject>Motility</subject><subject>Polyps</subject><subject>Proteins</subject><subject>Rhinosinusitis</subject><subject>Vimentin</subject><issn>2066-7655</issn><issn>2066-7663</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNo9jc1KxDAUhYsoOIzzDgHXhaRJ02Ypw_gDA25mP1zTG5shTWqS4swT-NpGFM_mnMXh-66qVUOlrDsp-fX_btvbapPSiZZwpWTTraqv3WzziM6Cq3OoJ0zo9XiZwJEcwSebbfAE_EBSmJDMEGHCjDGRYAiey0ejc4uDSCbI0Z5JxCkMBejfifVEjzF4q0kcrQ_J-uWHmMhnkRIPqWjm4C5zuqtuDLiEm79eV4fH3WH7XO9fn162D_t6Vn2u27fOKNagVEz1XHWK96LTjRZGotLABB0GyQVlFHpFwVAumDSSUyENVQr5urr_xc4xfCyY8vEUluiL8dgwxpvy7yX_BqnNYe0</recordid><startdate>20180901</startdate><enddate>20180901</enddate><creator>Onishchenko, Anatolii I</creator><creator>Lupyr, Andrii V</creator><creator>Tkachenko, Anton S</creator><creator>Gorbach, Tatyana V</creator><creator>Nakonechna, Oksana A</creator><creator>Gubina-Vakulyck, Galina I</creator><general>Bioflux SRL</general><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20180901</creationdate><title>Epithelial-to-mesenchymal transition and some parameters of extracellular matrix remodeling in chronic rhinosinusitis with nasal polyps</title><author>Onishchenko, Anatolii I ; Lupyr, Andrii V ; Tkachenko, Anton S ; Gorbach, Tatyana V ; Nakonechna, Oksana A ; Gubina-Vakulyck, Galina I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p98t-5b7f912e6919839793847c2c4f6e9ca140dd634010a890af03416f63046f099e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Age</topic><topic>Blood</topic><topic>Breast cancer</topic><topic>Cancer therapies</topic><topic>Cell adhesion & migration</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Epithelial cells</topic><topic>Extracellular matrix</topic><topic>Gelatinase B</topic><topic>Hypoxia</topic><topic>Inflammation</topic><topic>Lamina propria</topic><topic>Matrix metalloproteinase</topic><topic>Mesenchyme</topic><topic>Metalloproteinase</topic><topic>Metastasis</topic><topic>Monocyte chemoattractant protein</topic><topic>Monocyte chemoattractant protein 1</topic><topic>Monocytes</topic><topic>Motility</topic><topic>Polyps</topic><topic>Proteins</topic><topic>Rhinosinusitis</topic><topic>Vimentin</topic><toplevel>online_resources</toplevel><creatorcontrib>Onishchenko, Anatolii I</creatorcontrib><creatorcontrib>Lupyr, Andrii V</creatorcontrib><creatorcontrib>Tkachenko, Anton S</creatorcontrib><creatorcontrib>Gorbach, Tatyana V</creatorcontrib><creatorcontrib>Nakonechna, Oksana A</creatorcontrib><creatorcontrib>Gubina-Vakulyck, Galina I</creatorcontrib><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Human & veterinary medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Onishchenko, Anatolii I</au><au>Lupyr, Andrii V</au><au>Tkachenko, Anton S</au><au>Gorbach, Tatyana V</au><au>Nakonechna, Oksana A</au><au>Gubina-Vakulyck, Galina I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epithelial-to-mesenchymal transition and some parameters of extracellular matrix remodeling in chronic rhinosinusitis with nasal polyps</atitle><jtitle>Human & veterinary medicine</jtitle><date>2018-09-01</date><risdate>2018</risdate><volume>10</volume><issue>3</issue><spage>128</spage><epage>132</epage><pages>128-132</pages><issn>2066-7655</issn><eissn>2066-7663</eissn><abstract>Objective. The purpose of our study was to evaluate vimentin expression in nasal polyp tissue, concentrations of monocyte chemoattractant protein-1 (MCP-1) and matrix metalloproteinase-9 (MMP-9) in blood serum of patients with chronic rhinosinusitis with nasal polyps (CRSwNP).Materials and methods. Levels of MMP-9 and MCP-1 were determined in blood serum of twenty patients with CRSwNP using the commercially produced ELISA kits. Immunohistochemical vimentin staining using specimens of nasal polyp tissue was performed. The results were compared to the control group consisted of twenty relatively healthy subjects.Results. Vimentin was found to be strongly expressed in both the lamina propria and epithelial cells in nasal polyp tissue of patients with CRSwNP. Blood serum concentrations of MMP-9 and MCP-1 were higher in CRSwNP compared to the control individuals. MCP-1/MMP-9 ratio was higher as well. Conclusion. The development of nasal polyps is accompanied by an increase in the amount of vimentin-positive cells in the epithelial layer, which indicates the activation of endothelial-to-mesenchymal transition (EMT). CRSwNP is associated with the imbalance between profibrotic and antifibrotic factors.</abstract><cop>Cluj-Napoca</cop><pub>Bioflux SRL</pub><tpages>5</tpages></addata></record> |
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| subjects | Age Blood Breast cancer Cancer therapies Cell adhesion & migration Enzyme-linked immunosorbent assay Epithelial cells Extracellular matrix Gelatinase B Hypoxia Inflammation Lamina propria Matrix metalloproteinase Mesenchyme Metalloproteinase Metastasis Monocyte chemoattractant protein Monocyte chemoattractant protein 1 Monocytes Motility Polyps Proteins Rhinosinusitis Vimentin |
| title | Epithelial-to-mesenchymal transition and some parameters of extracellular matrix remodeling in chronic rhinosinusitis with nasal polyps |
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