Efficient preparation of narcissin from Opuntia ficus-indica fruits by combination of response surface methodology and high-speed countercurrent chromatography
Background: Narcissin is well known for their various biological activities. Objective: To recover narcissin from the Opuntia ficus-indica fruits (OFIF), an efficient method was developed by a combination of response surface methodology (RSM) and high-speed countercurrent chromatography (HSCCC). Mat...
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| Veröffentlicht in: | Pharmacognosy Magazine 2018-07, Vol.14 (56), p.338-343 |
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| creator | Yoo, Guijae Oh, Youngse Yang, Heejung Kim, Tae Sung, Sang Kim, Seung |
| description | Background: Narcissin is well known for their various biological activities. Objective: To recover narcissin from the Opuntia ficus-indica fruits (OFIF), an efficient method was developed by a combination of response surface methodology (RSM) and high-speed countercurrent chromatography (HSCCC). Materials and Methods: Optimization of extraction conditions of narcissin from OFIF was determined using RSM with three-level-three-factor Box-Behnken design (BBD). Then, a rapid and efficient method for the isolation of narcissin from the rich narcissin extracts was developed using HSCCC. Results: Regression analysis showed a good fit of the experimental data and the optimal condition was obtained as extraction time (X1), 6.02 h; solvent to material ratio (X2), 8.16 mL/g; and ethanol concentration (X3), 93.48%. Then, the rich narcissin extracts were separated by HSCCC with a two-phase solvent system composed of n-hexane: ethyl acetate: methanol: water (1.5:5:5:1.5, v/v/v/v) in one step within 60 min. As a result, 12 mg of narcissin was isolated from 100 mg of crude extract with purities of 98.5%, as determined by high-performance liquid chromatography (HPLC). Conclusion: This study can be useful to the development of industrial extraction processes, including further studies concerning the optimal number of sequential steps to enhance the efficacy of a large-scale extraction system.
Abbreviations used: BBD: Box-Behnken design; HPLC: High-performance liquid chromatography; HSCCC: High-speed countercurrent chromatography; OFIF: Opuntia ficus-indica fruits; RSM: Response surface methodology |
| doi_str_mv | 10.4103/pm.pm_422_17 |
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Abbreviations used: BBD: Box-Behnken design; HPLC: High-performance liquid chromatography; HSCCC: High-speed countercurrent chromatography; OFIF: Opuntia ficus-indica fruits; RSM: Response surface methodology</description><identifier>ISSN: 0973-1296</identifier><identifier>EISSN: 0976-4062</identifier><identifier>DOI: 10.4103/pm.pm_422_17</identifier><language>eng</language><publisher>London: Wolters Kluwer India Pvt. Ltd</publisher><subject>Chemical properties ; Chromatography ; Design ; Ethanol ; Flavonoids ; Fruits ; Health aspects ; NMR ; Nuclear magnetic resonance ; Pharmaceutical chemistry ; Pharmacognosy ; Pharmacological research ; Prickly pears ; Statistical analysis ; Variables ; Variance analysis</subject><ispartof>Pharmacognosy Magazine, 2018-07, Vol.14 (56), p.338-343</ispartof><rights>COPYRIGHT 2018 Medknow Publications and Media Pvt. Ltd.</rights><rights>2018. This work is published under https://creativecommons.org/licenses/by-nc-sa/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385n-16058ef1feb8ba4b20f513bd2caec7f71a68752dbc3d4c7c182d2a78e6d0da463</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids></links><search><creatorcontrib>Yoo, Guijae</creatorcontrib><creatorcontrib>Oh, Youngse</creatorcontrib><creatorcontrib>Yang, Heejung</creatorcontrib><creatorcontrib>Kim, Tae</creatorcontrib><creatorcontrib>Sung, Sang</creatorcontrib><creatorcontrib>Kim, Seung</creatorcontrib><title>Efficient preparation of narcissin from Opuntia ficus-indica fruits by combination of response surface methodology and high-speed countercurrent chromatography</title><title>Pharmacognosy Magazine</title><description>Background: Narcissin is well known for their various biological activities. Objective: To recover narcissin from the Opuntia ficus-indica fruits (OFIF), an efficient method was developed by a combination of response surface methodology (RSM) and high-speed countercurrent chromatography (HSCCC). Materials and Methods: Optimization of extraction conditions of narcissin from OFIF was determined using RSM with three-level-three-factor Box-Behnken design (BBD). Then, a rapid and efficient method for the isolation of narcissin from the rich narcissin extracts was developed using HSCCC. Results: Regression analysis showed a good fit of the experimental data and the optimal condition was obtained as extraction time (X1), 6.02 h; solvent to material ratio (X2), 8.16 mL/g; and ethanol concentration (X3), 93.48%. Then, the rich narcissin extracts were separated by HSCCC with a two-phase solvent system composed of n-hexane: ethyl acetate: methanol: water (1.5:5:5:1.5, v/v/v/v) in one step within 60 min. As a result, 12 mg of narcissin was isolated from 100 mg of crude extract with purities of 98.5%, as determined by high-performance liquid chromatography (HPLC). Conclusion: This study can be useful to the development of industrial extraction processes, including further studies concerning the optimal number of sequential steps to enhance the efficacy of a large-scale extraction system.
Abbreviations used: BBD: Box-Behnken design; HPLC: High-performance liquid chromatography; HSCCC: High-speed countercurrent chromatography; OFIF: Opuntia ficus-indica fruits; RSM: Response surface methodology</description><subject>Chemical properties</subject><subject>Chromatography</subject><subject>Design</subject><subject>Ethanol</subject><subject>Flavonoids</subject><subject>Fruits</subject><subject>Health aspects</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Pharmaceutical chemistry</subject><subject>Pharmacognosy</subject><subject>Pharmacological research</subject><subject>Prickly pears</subject><subject>Statistical analysis</subject><subject>Variables</subject><subject>Variance analysis</subject><issn>0973-1296</issn><issn>0976-4062</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptkctq3DAUhk1poWmaXR5A0G090cW2PJtCCEkaCGTTroUsHY2V2JJ6ZDPM0_RVq3TalELQQhe-7xxJf1WdM7ppGBUXad6kWTWcKybfVCd0K7u6oR1_-3stasa33fvqQ86PlLY9o_Kk-nntnDcewkISQtKoFx8DiY4Ejcbn7ANxGGfykNaweE0KvebaB-tN2eDql0yGAzFxHnx4kRFyiiEDySs6bYDMsIzRxinuDkQHS0a_G-ucAGxRS2VAsyI-X8OMpZ1e4g51Gg8fq3dOTxnO_syn1feb629XX-v7h9u7q8v72oi-DTXryoPAMQdDP-hm4NS1TAyWGw1GOsl018uW28EI2xhpWM8t17KHzlKrm06cVp-OdRPGHyvkRT3GFUNpqThjnHHZbdt_1E5PoHxwcUFtZp-NumxbKvt2K2ihNq9QZViYvYkBnC_n_wmfj4LBmDOCUwn9rPGgGFXPyaoS60uyBf9yxPdxKh-Xn6Z1D6hmsE8h7l91lBC9-hu1-AWlTLGA</recordid><startdate>20180701</startdate><enddate>20180701</enddate><creator>Yoo, Guijae</creator><creator>Oh, Youngse</creator><creator>Yang, Heejung</creator><creator>Kim, Tae</creator><creator>Sung, Sang</creator><creator>Kim, Seung</creator><general>Wolters Kluwer India Pvt. 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Objective: To recover narcissin from the Opuntia ficus-indica fruits (OFIF), an efficient method was developed by a combination of response surface methodology (RSM) and high-speed countercurrent chromatography (HSCCC). Materials and Methods: Optimization of extraction conditions of narcissin from OFIF was determined using RSM with three-level-three-factor Box-Behnken design (BBD). Then, a rapid and efficient method for the isolation of narcissin from the rich narcissin extracts was developed using HSCCC. Results: Regression analysis showed a good fit of the experimental data and the optimal condition was obtained as extraction time (X1), 6.02 h; solvent to material ratio (X2), 8.16 mL/g; and ethanol concentration (X3), 93.48%. Then, the rich narcissin extracts were separated by HSCCC with a two-phase solvent system composed of n-hexane: ethyl acetate: methanol: water (1.5:5:5:1.5, v/v/v/v) in one step within 60 min. As a result, 12 mg of narcissin was isolated from 100 mg of crude extract with purities of 98.5%, as determined by high-performance liquid chromatography (HPLC). Conclusion: This study can be useful to the development of industrial extraction processes, including further studies concerning the optimal number of sequential steps to enhance the efficacy of a large-scale extraction system.
Abbreviations used: BBD: Box-Behnken design; HPLC: High-performance liquid chromatography; HSCCC: High-speed countercurrent chromatography; OFIF: Opuntia ficus-indica fruits; RSM: Response surface methodology</abstract><cop>London</cop><pub>Wolters Kluwer India Pvt. Ltd</pub><doi>10.4103/pm.pm_422_17</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Chemical properties Chromatography Design Ethanol Flavonoids Fruits Health aspects NMR Nuclear magnetic resonance Pharmaceutical chemistry Pharmacognosy Pharmacological research Prickly pears Statistical analysis Variables Variance analysis |
| title | Efficient preparation of narcissin from Opuntia ficus-indica fruits by combination of response surface methodology and high-speed countercurrent chromatography |
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