Pilot‐scale development of core–shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids
ABSTRACT A recombinant lipase B from Candida antarctica (LipB) in Pichia pastoris was synthesized through submerged fermentation using crude glycerin as substrate. The immobilization of this enzyme on the core–shell polymeric supports is an effective alternative for its application. The supports wit...
Gespeichert in:
| Veröffentlicht in: | Journal of applied polymer science 2018-10, Vol.135 (40), p.n/a |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | n/a |
|---|---|
| container_issue | 40 |
| container_start_page | |
| container_title | Journal of applied polymer science |
| container_volume | 135 |
| creator | Cipolatti, Eliane Pereira Pinto, Martina Costa Cerqueira Robert, Julia de Macedo da Silva, Tabita Pizoni Beralto, Thamires da Costa Santos, Jorge G. F. de Castro, Rui de Paula Vieira Fernandez‐Lafuente, Roberto Manoel, Evelin Andrade Pinto, José Carlos Freire, Denise Maria Guimarães |
| description | ABSTRACT
A recombinant lipase B from Candida antarctica (LipB) in Pichia pastoris was synthesized through submerged fermentation using crude glycerin as substrate. The immobilization of this enzyme on the core–shell polymeric supports is an effective alternative for its application. The supports with distinct levels of hydrophobicity were produced through combined suspension and emulsion polymerization in pilot scale. Particles with distinct compositions were synthesized (PMMA/PMMA; PMMA‐co‐DVB/PMMA‐co‐DVB; and PS‐co‐DVB/PS‐co‐DVB) and employed on the immobilization of the produced lipase (LipB) and the commercial enzyme (CalB). The morphological properties (specific area, average pore diameter, specific volume of pores, and hydrophobicity level) and the influence of the polymerization conditions on the morphology of the supports were studied. The thermal stability of such biocatalysts was also investigated in the presence of calcium cation (Ca+2), maintained 100% of the activity after 3 h at 50°C when the PMMA‐co‐DVB/PMMA‐co‐DVB was employed. The synthesized enzyme and supports manufactured in pilot scale were employed successfully for production of esters using residual fatty acids as substrates, adding value to these raw materials and increasing the ranges of possible applications. |
| doi_str_mv | 10.1002/app.46727 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2111771461</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2111771461</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3347-f4d61b9041a795df6268f14aeaee7de1643d7769099a64b3fcdd3583b73475053</originalsourceid><addsrcrecordid>eNp1kcFu1DAQhi1EJZbSQ9_AEicOae3EsTfHsoIWqRJ7oOfIscdaV05sbAcUTn0EJB6NN-iT4N3AkZNHnv__5h8NQpeUXFFC6msZwhXjohYv0IaSTlSM19uXaFN6tNp2XfsKvU7pkRBKW8I36PfeOp-fn34mJR1gDd_A-TDClLE3WPkIz0-_0gGcw8G7ZYSI0xyCjzlh4yPOB8B2HP1gnf0hs_XT0RdB-XGwkywYZ4NMgN9jE_2Id3LSVktcOjKqbNWx1EeMjbiEd-XnRLHTiR2i17P6x4V8WByGlCGmlRchWT1Lh43MecFSWZ3eoDMjXYKLv-85evj44cvurrr_fPtpd3NfqaZhojJMczp0hFEpulYbXvOtoUyCBBAaKGeNFoJ3pOskZ0NjlNZNu20GUdwtaZtz9HbllpBf55Kqf_RznMrIvqaUCkEZp0X1blWp6FOKYPoQ7Sjj0lPSH2_Wl7X7082K9nrVfrcOlv8L-5v9fnX8AbVRoAQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2111771461</pqid></control><display><type>article</type><title>Pilot‐scale development of core–shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids</title><source>Wiley Online Library Journals Frontfile Complete</source><creator>Cipolatti, Eliane Pereira ; Pinto, Martina Costa Cerqueira ; Robert, Julia de Macedo ; da Silva, Tabita Pizoni ; Beralto, Thamires da Costa ; Santos, Jorge G. F. ; de Castro, Rui de Paula Vieira ; Fernandez‐Lafuente, Roberto ; Manoel, Evelin Andrade ; Pinto, José Carlos ; Freire, Denise Maria Guimarães</creator><creatorcontrib>Cipolatti, Eliane Pereira ; Pinto, Martina Costa Cerqueira ; Robert, Julia de Macedo ; da Silva, Tabita Pizoni ; Beralto, Thamires da Costa ; Santos, Jorge G. F. ; de Castro, Rui de Paula Vieira ; Fernandez‐Lafuente, Roberto ; Manoel, Evelin Andrade ; Pinto, José Carlos ; Freire, Denise Maria Guimarães</creatorcontrib><description>ABSTRACT
A recombinant lipase B from Candida antarctica (LipB) in Pichia pastoris was synthesized through submerged fermentation using crude glycerin as substrate. The immobilization of this enzyme on the core–shell polymeric supports is an effective alternative for its application. The supports with distinct levels of hydrophobicity were produced through combined suspension and emulsion polymerization in pilot scale. Particles with distinct compositions were synthesized (PMMA/PMMA; PMMA‐co‐DVB/PMMA‐co‐DVB; and PS‐co‐DVB/PS‐co‐DVB) and employed on the immobilization of the produced lipase (LipB) and the commercial enzyme (CalB). The morphological properties (specific area, average pore diameter, specific volume of pores, and hydrophobicity level) and the influence of the polymerization conditions on the morphology of the supports were studied. The thermal stability of such biocatalysts was also investigated in the presence of calcium cation (Ca+2), maintained 100% of the activity after 3 h at 50°C when the PMMA‐co‐DVB/PMMA‐co‐DVB was employed. The synthesized enzyme and supports manufactured in pilot scale were employed successfully for production of esters using residual fatty acids as substrates, adding value to these raw materials and increasing the ranges of possible applications.</description><identifier>ISSN: 0021-8995</identifier><identifier>EISSN: 1097-4628</identifier><identifier>DOI: 10.1002/app.46727</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>biopolymers and renewable polymers ; Calcium ions ; Chemical synthesis ; Emulsion polymerization ; Enzymes ; Esters ; Ethyl esters ; Fatty acids ; Fermentation ; Hydrophobicity ; Immobilization ; Lipase ; Materials science ; microscopy ; Morphology ; Polymerization ; Polymers ; Polymethyl methacrylate ; polystyrene ; porous materials ; Raw materials ; Specific volume ; Substrates ; Thermal stability ; Yeast</subject><ispartof>Journal of applied polymer science, 2018-10, Vol.135 (40), p.n/a</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3347-f4d61b9041a795df6268f14aeaee7de1643d7769099a64b3fcdd3583b73475053</citedby><cites>FETCH-LOGICAL-c3347-f4d61b9041a795df6268f14aeaee7de1643d7769099a64b3fcdd3583b73475053</cites><orcidid>0000-0002-3272-712X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fapp.46727$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fapp.46727$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids></links><search><creatorcontrib>Cipolatti, Eliane Pereira</creatorcontrib><creatorcontrib>Pinto, Martina Costa Cerqueira</creatorcontrib><creatorcontrib>Robert, Julia de Macedo</creatorcontrib><creatorcontrib>da Silva, Tabita Pizoni</creatorcontrib><creatorcontrib>Beralto, Thamires da Costa</creatorcontrib><creatorcontrib>Santos, Jorge G. F.</creatorcontrib><creatorcontrib>de Castro, Rui de Paula Vieira</creatorcontrib><creatorcontrib>Fernandez‐Lafuente, Roberto</creatorcontrib><creatorcontrib>Manoel, Evelin Andrade</creatorcontrib><creatorcontrib>Pinto, José Carlos</creatorcontrib><creatorcontrib>Freire, Denise Maria Guimarães</creatorcontrib><title>Pilot‐scale development of core–shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids</title><title>Journal of applied polymer science</title><description>ABSTRACT
A recombinant lipase B from Candida antarctica (LipB) in Pichia pastoris was synthesized through submerged fermentation using crude glycerin as substrate. The immobilization of this enzyme on the core–shell polymeric supports is an effective alternative for its application. The supports with distinct levels of hydrophobicity were produced through combined suspension and emulsion polymerization in pilot scale. Particles with distinct compositions were synthesized (PMMA/PMMA; PMMA‐co‐DVB/PMMA‐co‐DVB; and PS‐co‐DVB/PS‐co‐DVB) and employed on the immobilization of the produced lipase (LipB) and the commercial enzyme (CalB). The morphological properties (specific area, average pore diameter, specific volume of pores, and hydrophobicity level) and the influence of the polymerization conditions on the morphology of the supports were studied. The thermal stability of such biocatalysts was also investigated in the presence of calcium cation (Ca+2), maintained 100% of the activity after 3 h at 50°C when the PMMA‐co‐DVB/PMMA‐co‐DVB was employed. The synthesized enzyme and supports manufactured in pilot scale were employed successfully for production of esters using residual fatty acids as substrates, adding value to these raw materials and increasing the ranges of possible applications.</description><subject>biopolymers and renewable polymers</subject><subject>Calcium ions</subject><subject>Chemical synthesis</subject><subject>Emulsion polymerization</subject><subject>Enzymes</subject><subject>Esters</subject><subject>Ethyl esters</subject><subject>Fatty acids</subject><subject>Fermentation</subject><subject>Hydrophobicity</subject><subject>Immobilization</subject><subject>Lipase</subject><subject>Materials science</subject><subject>microscopy</subject><subject>Morphology</subject><subject>Polymerization</subject><subject>Polymers</subject><subject>Polymethyl methacrylate</subject><subject>polystyrene</subject><subject>porous materials</subject><subject>Raw materials</subject><subject>Specific volume</subject><subject>Substrates</subject><subject>Thermal stability</subject><subject>Yeast</subject><issn>0021-8995</issn><issn>1097-4628</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kcFu1DAQhi1EJZbSQ9_AEicOae3EsTfHsoIWqRJ7oOfIscdaV05sbAcUTn0EJB6NN-iT4N3AkZNHnv__5h8NQpeUXFFC6msZwhXjohYv0IaSTlSM19uXaFN6tNp2XfsKvU7pkRBKW8I36PfeOp-fn34mJR1gDd_A-TDClLE3WPkIz0-_0gGcw8G7ZYSI0xyCjzlh4yPOB8B2HP1gnf0hs_XT0RdB-XGwkywYZ4NMgN9jE_2Id3LSVktcOjKqbNWx1EeMjbiEd-XnRLHTiR2i17P6x4V8WByGlCGmlRchWT1Lh43MecFSWZ3eoDMjXYKLv-85evj44cvurrr_fPtpd3NfqaZhojJMczp0hFEpulYbXvOtoUyCBBAaKGeNFoJ3pOskZ0NjlNZNu20GUdwtaZtz9HbllpBf55Kqf_RznMrIvqaUCkEZp0X1blWp6FOKYPoQ7Sjj0lPSH2_Wl7X7082K9nrVfrcOlv8L-5v9fnX8AbVRoAQ</recordid><startdate>20181020</startdate><enddate>20181020</enddate><creator>Cipolatti, Eliane Pereira</creator><creator>Pinto, Martina Costa Cerqueira</creator><creator>Robert, Julia de Macedo</creator><creator>da Silva, Tabita Pizoni</creator><creator>Beralto, Thamires da Costa</creator><creator>Santos, Jorge G. F.</creator><creator>de Castro, Rui de Paula Vieira</creator><creator>Fernandez‐Lafuente, Roberto</creator><creator>Manoel, Evelin Andrade</creator><creator>Pinto, José Carlos</creator><creator>Freire, Denise Maria Guimarães</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>JG9</scope><orcidid>https://orcid.org/0000-0002-3272-712X</orcidid></search><sort><creationdate>20181020</creationdate><title>Pilot‐scale development of core–shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids</title><author>Cipolatti, Eliane Pereira ; Pinto, Martina Costa Cerqueira ; Robert, Julia de Macedo ; da Silva, Tabita Pizoni ; Beralto, Thamires da Costa ; Santos, Jorge G. F. ; de Castro, Rui de Paula Vieira ; Fernandez‐Lafuente, Roberto ; Manoel, Evelin Andrade ; Pinto, José Carlos ; Freire, Denise Maria Guimarães</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3347-f4d61b9041a795df6268f14aeaee7de1643d7769099a64b3fcdd3583b73475053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>biopolymers and renewable polymers</topic><topic>Calcium ions</topic><topic>Chemical synthesis</topic><topic>Emulsion polymerization</topic><topic>Enzymes</topic><topic>Esters</topic><topic>Ethyl esters</topic><topic>Fatty acids</topic><topic>Fermentation</topic><topic>Hydrophobicity</topic><topic>Immobilization</topic><topic>Lipase</topic><topic>Materials science</topic><topic>microscopy</topic><topic>Morphology</topic><topic>Polymerization</topic><topic>Polymers</topic><topic>Polymethyl methacrylate</topic><topic>polystyrene</topic><topic>porous materials</topic><topic>Raw materials</topic><topic>Specific volume</topic><topic>Substrates</topic><topic>Thermal stability</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cipolatti, Eliane Pereira</creatorcontrib><creatorcontrib>Pinto, Martina Costa Cerqueira</creatorcontrib><creatorcontrib>Robert, Julia de Macedo</creatorcontrib><creatorcontrib>da Silva, Tabita Pizoni</creatorcontrib><creatorcontrib>Beralto, Thamires da Costa</creatorcontrib><creatorcontrib>Santos, Jorge G. F.</creatorcontrib><creatorcontrib>de Castro, Rui de Paula Vieira</creatorcontrib><creatorcontrib>Fernandez‐Lafuente, Roberto</creatorcontrib><creatorcontrib>Manoel, Evelin Andrade</creatorcontrib><creatorcontrib>Pinto, José Carlos</creatorcontrib><creatorcontrib>Freire, Denise Maria Guimarães</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Journal of applied polymer science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cipolatti, Eliane Pereira</au><au>Pinto, Martina Costa Cerqueira</au><au>Robert, Julia de Macedo</au><au>da Silva, Tabita Pizoni</au><au>Beralto, Thamires da Costa</au><au>Santos, Jorge G. F.</au><au>de Castro, Rui de Paula Vieira</au><au>Fernandez‐Lafuente, Roberto</au><au>Manoel, Evelin Andrade</au><au>Pinto, José Carlos</au><au>Freire, Denise Maria Guimarães</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pilot‐scale development of core–shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids</atitle><jtitle>Journal of applied polymer science</jtitle><date>2018-10-20</date><risdate>2018</risdate><volume>135</volume><issue>40</issue><epage>n/a</epage><issn>0021-8995</issn><eissn>1097-4628</eissn><abstract>ABSTRACT
A recombinant lipase B from Candida antarctica (LipB) in Pichia pastoris was synthesized through submerged fermentation using crude glycerin as substrate. The immobilization of this enzyme on the core–shell polymeric supports is an effective alternative for its application. The supports with distinct levels of hydrophobicity were produced through combined suspension and emulsion polymerization in pilot scale. Particles with distinct compositions were synthesized (PMMA/PMMA; PMMA‐co‐DVB/PMMA‐co‐DVB; and PS‐co‐DVB/PS‐co‐DVB) and employed on the immobilization of the produced lipase (LipB) and the commercial enzyme (CalB). The morphological properties (specific area, average pore diameter, specific volume of pores, and hydrophobicity level) and the influence of the polymerization conditions on the morphology of the supports were studied. The thermal stability of such biocatalysts was also investigated in the presence of calcium cation (Ca+2), maintained 100% of the activity after 3 h at 50°C when the PMMA‐co‐DVB/PMMA‐co‐DVB was employed. The synthesized enzyme and supports manufactured in pilot scale were employed successfully for production of esters using residual fatty acids as substrates, adding value to these raw materials and increasing the ranges of possible applications.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><doi>10.1002/app.46727</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-3272-712X</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8995 |
| ispartof | Journal of applied polymer science, 2018-10, Vol.135 (40), p.n/a |
| issn | 0021-8995 1097-4628 |
| language | eng |
| recordid | cdi_proquest_journals_2111771461 |
| source | Wiley Online Library Journals Frontfile Complete |
| subjects | biopolymers and renewable polymers Calcium ions Chemical synthesis Emulsion polymerization Enzymes Esters Ethyl esters Fatty acids Fermentation Hydrophobicity Immobilization Lipase Materials science microscopy Morphology Polymerization Polymers Polymethyl methacrylate polystyrene porous materials Raw materials Specific volume Substrates Thermal stability Yeast |
| title | Pilot‐scale development of core–shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T22%3A17%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pilot%E2%80%90scale%20development%20of%20core%E2%80%93shell%20polymer%20supports%20for%20the%20immobilization%20of%20recombinant%20lipase%20B%20from%20Candida%20antarctica%20and%20their%20application%20in%20the%20production%20of%20ethyl%20esters%20from%20residual%20fatty%20acids&rft.jtitle=Journal%20of%20applied%20polymer%20science&rft.au=Cipolatti,%20Eliane%20Pereira&rft.date=2018-10-20&rft.volume=135&rft.issue=40&rft.epage=n/a&rft.issn=0021-8995&rft.eissn=1097-4628&rft_id=info:doi/10.1002/app.46727&rft_dat=%3Cproquest_cross%3E2111771461%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2111771461&rft_id=info:pmid/&rfr_iscdi=true |