Gold nanorod-based fluorometric ELISA for the sensitive detection of a cancer biomarker

The combinatorial use of an enzyme-linked immunosorbent assay (ELISA) and nanoparticles ( i.e. nanoELISA) facilitates the improved detection of biomarkers. In the present study, we have developed a gold nanorod-based fluorometric immunoassay (nanoELIFA) for the non-invasive detection of CA125 (an ov...

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Veröffentlicht in:	New journal of chemistry 2018, Vol.42 (19), p.15852-15859
Hauptverfasser:	Alex, Sruthi Ann, Chandrasekaran, N., Mukherjee, Amitava
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Sprache:	eng
Schlagworte:	Biomarkers Blood plasma Cancer Combinatorial analysis Fluorescence Gold Immunoassay Nanoparticles Nanorods Reproducibility Sensitivity analysis Substrates
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creator	Alex, Sruthi Ann Chandrasekaran, N. Mukherjee, Amitava
description	The combinatorial use of an enzyme-linked immunosorbent assay (ELISA) and nanoparticles ( i.e. nanoELISA) facilitates the improved detection of biomarkers. In the present study, we have developed a gold nanorod-based fluorometric immunoassay (nanoELIFA) for the non-invasive detection of CA125 (an ovarian cancer biomarker) using a fluorogenic substrate, 3- p -hydroxyphenylpropionic acid (HPPA). NanoELIFA showed improved sensitivity (detection limit 0.066 U mL −1 ) over classical ELIFA (detection limit: 0.23 U mL −1 ) due to the integration of gold nanorods, which act as a signal enhancer with an additional advantage of fluorescence-based detection for improved sensitivity. The sensitivity of the analyses was tested in the range of 0–400 U mL −1 . Furthermore, the reproducibility and selectivity of nanoELIFA were analyzed and indicative of the good performance of the system. This method was initially tested using spiked simulated blood plasma and cell culture media, and finally, was analyzed in plasma (as such and spiked) and supernatant media from in vitro cell samples. The good recovery percentages indicated in all these cases highlighted the applicability of the developed nanoELIFA for real sample analysis.
doi_str_mv	10.1039/C8NJ03467B
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source	Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection
subjects	Biomarkers Blood plasma Cancer Combinatorial analysis Fluorescence Gold Immunoassay Nanoparticles Nanorods Reproducibility Sensitivity analysis Substrates
title	Gold nanorod-based fluorometric ELISA for the sensitive detection of a cancer biomarker
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