A solid-state Sb/Sb2O3 biosensor for the in situ measurement of extracellular acidification associated with the multidrug resistance phenotype in breast cancer cells
Metal oxide-based pH sensors have received considerable attention owing to their advantages and potential applications in biomedicine, materials science, environmental science, and analytical science. The occurrence of multidrug resistance (MDR) hinders the efficiency of chemotherapy. In addition, a...
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| Veröffentlicht in: | Analytical methods 2018-09, Vol.10 (36), p.4445-4453 |
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| creator | Li, Zhen Li, Zong Liu, Hanmeng Yao, Zhixia Sun, Yujing Zhuang, Li |
| description | Metal oxide-based pH sensors have received considerable attention owing to their advantages and potential applications in biomedicine, materials science, environmental science, and analytical science. The occurrence of multidrug resistance (MDR) hinders the efficiency of chemotherapy. In addition, a low extracellular pH (pHe), which is a common feature of the tumor microenvironment, contributes to resistance to chemotherapy. Therefore, in this work we demonstrate the facile fabrication of a solid-state Sb/Sb2O3 (SSO) electrode for high-performance pH sensing to monitor the pHe of two cell lines (MCF-7/ADR Adriamycin-resistant breast cancer cells and their MCF-7 parent cells). To achieve this aim, the electrode was prepared by melting antimony (Sb) in a U-type glass tube and subsequent oxidation via the molten sodium nitrate method. The fabricated SSO electrode was characterized by scanning electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. It was found that the SSO electrode-based pH sensor exhibited high performance with a sensitivity of −57.30 ± 0.60 mV pH−1 and a correlation coefficient (R2) of greater than 0.9966 in aqueous buffer solutions (pH = 2–12). In addition, the fabricated pH sensor displayed not only a short response time (about 2 s) but also long-term stability (pH deviation of |
| doi_str_mv | 10.1039/c8ay01458b |
| format | Article |
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The occurrence of multidrug resistance (MDR) hinders the efficiency of chemotherapy. In addition, a low extracellular pH (pHe), which is a common feature of the tumor microenvironment, contributes to resistance to chemotherapy. Therefore, in this work we demonstrate the facile fabrication of a solid-state Sb/Sb2O3 (SSO) electrode for high-performance pH sensing to monitor the pHe of two cell lines (MCF-7/ADR Adriamycin-resistant breast cancer cells and their MCF-7 parent cells). To achieve this aim, the electrode was prepared by melting antimony (Sb) in a U-type glass tube and subsequent oxidation via the molten sodium nitrate method. The fabricated SSO electrode was characterized by scanning electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. It was found that the SSO electrode-based pH sensor exhibited high performance with a sensitivity of −57.30 ± 0.60 mV pH−1 and a correlation coefficient (R2) of greater than 0.9966 in aqueous buffer solutions (pH = 2–12). In addition, the fabricated pH sensor displayed not only a short response time (about 2 s) but also long-term stability (pH deviation of <0.15 after three months). Furthermore, the differences in pHe between MCF-7/ADR and MCF-7 cells were determined by the fabricated SSO electrode, and MCF-7/ADR cells and MCF-7 cells maintained pHe values of about 6.83 and 7.02, respectively, after 12 h. The results showed that the fabricated SSO electrode could be used to measure differences in pHe and also used to monitor the metabolic activity of cells.</description><identifier>ISSN: 1759-9660</identifier><identifier>EISSN: 1759-9679</identifier><identifier>DOI: 10.1039/c8ay01458b</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Acidification ; Antimony ; Antimony trioxide ; Biosensors ; Breast cancer ; Buffer solutions ; Cancer ; Chemotherapy ; Correlation coefficient ; Correlation coefficients ; Electrodes ; Environmental science ; Fabrication ; In situ measurement ; Materials science ; Mathematical analysis ; Multidrug resistance ; Oxidation ; pH effects ; pH sensors ; Phenotypes ; Photoelectron spectroscopy ; Response time ; Scanning electron microscopy ; Sodium ; Sodium nitrate ; Solid state ; X-ray diffraction</subject><ispartof>Analytical methods, 2018-09, Vol.10 (36), p.4445-4453</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Li, Zhen</creatorcontrib><creatorcontrib>Li, Zong</creatorcontrib><creatorcontrib>Liu, Hanmeng</creatorcontrib><creatorcontrib>Yao, Zhixia</creatorcontrib><creatorcontrib>Sun, Yujing</creatorcontrib><creatorcontrib>Zhuang, Li</creatorcontrib><title>A solid-state Sb/Sb2O3 biosensor for the in situ measurement of extracellular acidification associated with the multidrug resistance phenotype in breast cancer cells</title><title>Analytical methods</title><description>Metal oxide-based pH sensors have received considerable attention owing to their advantages and potential applications in biomedicine, materials science, environmental science, and analytical science. The occurrence of multidrug resistance (MDR) hinders the efficiency of chemotherapy. In addition, a low extracellular pH (pHe), which is a common feature of the tumor microenvironment, contributes to resistance to chemotherapy. Therefore, in this work we demonstrate the facile fabrication of a solid-state Sb/Sb2O3 (SSO) electrode for high-performance pH sensing to monitor the pHe of two cell lines (MCF-7/ADR Adriamycin-resistant breast cancer cells and their MCF-7 parent cells). To achieve this aim, the electrode was prepared by melting antimony (Sb) in a U-type glass tube and subsequent oxidation via the molten sodium nitrate method. The fabricated SSO electrode was characterized by scanning electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. It was found that the SSO electrode-based pH sensor exhibited high performance with a sensitivity of −57.30 ± 0.60 mV pH−1 and a correlation coefficient (R2) of greater than 0.9966 in aqueous buffer solutions (pH = 2–12). In addition, the fabricated pH sensor displayed not only a short response time (about 2 s) but also long-term stability (pH deviation of <0.15 after three months). Furthermore, the differences in pHe between MCF-7/ADR and MCF-7 cells were determined by the fabricated SSO electrode, and MCF-7/ADR cells and MCF-7 cells maintained pHe values of about 6.83 and 7.02, respectively, after 12 h. The results showed that the fabricated SSO electrode could be used to measure differences in pHe and also used to monitor the metabolic activity of cells.</description><subject>Acidification</subject><subject>Antimony</subject><subject>Antimony trioxide</subject><subject>Biosensors</subject><subject>Breast cancer</subject><subject>Buffer solutions</subject><subject>Cancer</subject><subject>Chemotherapy</subject><subject>Correlation coefficient</subject><subject>Correlation coefficients</subject><subject>Electrodes</subject><subject>Environmental science</subject><subject>Fabrication</subject><subject>In situ measurement</subject><subject>Materials science</subject><subject>Mathematical analysis</subject><subject>Multidrug resistance</subject><subject>Oxidation</subject><subject>pH effects</subject><subject>pH sensors</subject><subject>Phenotypes</subject><subject>Photoelectron spectroscopy</subject><subject>Response time</subject><subject>Scanning electron microscopy</subject><subject>Sodium</subject><subject>Sodium nitrate</subject><subject>Solid state</subject><subject>X-ray diffraction</subject><issn>1759-9660</issn><issn>1759-9679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNo9j81KAzEUhYMoWKsbn-CC67H5m8lkWYpaodBFdV2STNKmTCc1yaB9IN_TaRUXl3PgwPdxEbon-JFgJiemVkdMeFnrCzQiopSFrIS8_O8VvkY3Ke0wriSryAh9TyGF1jdFyipbWOnJStMlA-1Dsl0KEdxweWvBd5B87mFvVeqj3dsuQ3Bgv3JUxrZt36oIyvjGO29U9qEDlVIwfuA28Onz9ozZ9232Tew3EG3yg7UzFg5b24V8PJwtOg6GDOa0RDih0y26cqpN9u4vx-j9-eltNi8Wy5fX2XRRbCjFefiPOMZNRal0xCpaMs5rrnWN67IR3HHmODUlFrIUtTWVo9pwJytDVImdFmyMHn65hxg-epvyehf62A3KNSUEU8EFqdkPrddwQA</recordid><startdate>20180928</startdate><enddate>20180928</enddate><creator>Li, Zhen</creator><creator>Li, Zong</creator><creator>Liu, Hanmeng</creator><creator>Yao, Zhixia</creator><creator>Sun, Yujing</creator><creator>Zhuang, Li</creator><general>Royal Society of Chemistry</general><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope></search><sort><creationdate>20180928</creationdate><title>A solid-state Sb/Sb2O3 biosensor for the in situ measurement of extracellular acidification associated with the multidrug resistance phenotype in breast cancer cells</title><author>Li, Zhen ; Li, Zong ; Liu, Hanmeng ; Yao, Zhixia ; Sun, Yujing ; Zhuang, Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g220t-961f34c6229f1ea2534484bb8085d74f43f42c5079578ec6f2bc4f96c1a50fb73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acidification</topic><topic>Antimony</topic><topic>Antimony trioxide</topic><topic>Biosensors</topic><topic>Breast cancer</topic><topic>Buffer solutions</topic><topic>Cancer</topic><topic>Chemotherapy</topic><topic>Correlation coefficient</topic><topic>Correlation coefficients</topic><topic>Electrodes</topic><topic>Environmental science</topic><topic>Fabrication</topic><topic>In situ measurement</topic><topic>Materials science</topic><topic>Mathematical analysis</topic><topic>Multidrug resistance</topic><topic>Oxidation</topic><topic>pH effects</topic><topic>pH sensors</topic><topic>Phenotypes</topic><topic>Photoelectron spectroscopy</topic><topic>Response time</topic><topic>Scanning electron microscopy</topic><topic>Sodium</topic><topic>Sodium nitrate</topic><topic>Solid state</topic><topic>X-ray diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Zhen</creatorcontrib><creatorcontrib>Li, Zong</creatorcontrib><creatorcontrib>Liu, Hanmeng</creatorcontrib><creatorcontrib>Yao, Zhixia</creatorcontrib><creatorcontrib>Sun, Yujing</creatorcontrib><creatorcontrib>Zhuang, Li</creatorcontrib><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Analytical methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Zhen</au><au>Li, Zong</au><au>Liu, Hanmeng</au><au>Yao, Zhixia</au><au>Sun, Yujing</au><au>Zhuang, Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A solid-state Sb/Sb2O3 biosensor for the in situ measurement of extracellular acidification associated with the multidrug resistance phenotype in breast cancer cells</atitle><jtitle>Analytical methods</jtitle><date>2018-09-28</date><risdate>2018</risdate><volume>10</volume><issue>36</issue><spage>4445</spage><epage>4453</epage><pages>4445-4453</pages><issn>1759-9660</issn><eissn>1759-9679</eissn><abstract>Metal oxide-based pH sensors have received considerable attention owing to their advantages and potential applications in biomedicine, materials science, environmental science, and analytical science. The occurrence of multidrug resistance (MDR) hinders the efficiency of chemotherapy. In addition, a low extracellular pH (pHe), which is a common feature of the tumor microenvironment, contributes to resistance to chemotherapy. Therefore, in this work we demonstrate the facile fabrication of a solid-state Sb/Sb2O3 (SSO) electrode for high-performance pH sensing to monitor the pHe of two cell lines (MCF-7/ADR Adriamycin-resistant breast cancer cells and their MCF-7 parent cells). To achieve this aim, the electrode was prepared by melting antimony (Sb) in a U-type glass tube and subsequent oxidation via the molten sodium nitrate method. The fabricated SSO electrode was characterized by scanning electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. It was found that the SSO electrode-based pH sensor exhibited high performance with a sensitivity of −57.30 ± 0.60 mV pH−1 and a correlation coefficient (R2) of greater than 0.9966 in aqueous buffer solutions (pH = 2–12). In addition, the fabricated pH sensor displayed not only a short response time (about 2 s) but also long-term stability (pH deviation of <0.15 after three months). Furthermore, the differences in pHe between MCF-7/ADR and MCF-7 cells were determined by the fabricated SSO electrode, and MCF-7/ADR cells and MCF-7 cells maintained pHe values of about 6.83 and 7.02, respectively, after 12 h. The results showed that the fabricated SSO electrode could be used to measure differences in pHe and also used to monitor the metabolic activity of cells.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/c8ay01458b</doi><tpages>9</tpages></addata></record> |
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| source | Royal Society Of Chemistry Journals 2008- |
| subjects | Acidification Antimony Antimony trioxide Biosensors Breast cancer Buffer solutions Cancer Chemotherapy Correlation coefficient Correlation coefficients Electrodes Environmental science Fabrication In situ measurement Materials science Mathematical analysis Multidrug resistance Oxidation pH effects pH sensors Phenotypes Photoelectron spectroscopy Response time Scanning electron microscopy Sodium Sodium nitrate Solid state X-ray diffraction |
| title | A solid-state Sb/Sb2O3 biosensor for the in situ measurement of extracellular acidification associated with the multidrug resistance phenotype in breast cancer cells |
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