The Dose Rate of UVA Treatment Influences the Cellular Response of HaCaT Keratinocytes
The contribution of UV exposure to the etiology of skin cancer and photoaging is undisputed. However, the effect of altering the intensity or dose rate of UV, which varies considerably with geographical location, the time of day or year, and the use of sunscreens, is not understood. In this study, t...
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| Veröffentlicht in: | Journal of investigative dermatology 2008-03, Vol.128 (3), p.685-693 |
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| creator | Shorrocks, Julie Paul, Nigel D. McMillan, Trevor J. |
| description | The contribution of UV exposure to the etiology of skin cancer and photoaging is undisputed. However, the effect of altering the intensity or dose rate of UV, which varies considerably with geographical location, the time of day or year, and the use of sunscreens, is not understood. In this study, the effect of altering the dose rate of UVA was investigated in the immortalized human keratinocyte cell line, HaCaT. Lowering the dose rate of UVA resulted in increased cytotoxicity, which correlated with increases in both lipid peroxidation and DNA damage. Furthermore, exposure at low dose rate did not appear to reduce the ability of UVA to induce the phenomenon of persistent genomic instability. Pretreatment with the antioxidant vitamin E significantly protected against UVA dose-rate effects observed with respect to lipid peroxidation and survival. Additionally, cell populations irradiated at low dose rate exhibited a shift towards a more pro-oxidant state. Taken together, these observations suggest an oxidative stress mechanism is underlying the UVA dose-rate effect. This study demonstrates that dose rates must be included as a key factor when evaluating the biological effects of UVA, especially considering the concerns, which exist regarding the efficacy and photostability of sunscreens to UVA. |
| doi_str_mv | 10.1038/sj.jid.5701037 |
| format | Article |
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However, the effect of altering the intensity or dose rate of UV, which varies considerably with geographical location, the time of day or year, and the use of sunscreens, is not understood. In this study, the effect of altering the dose rate of UVA was investigated in the immortalized human keratinocyte cell line, HaCaT. Lowering the dose rate of UVA resulted in increased cytotoxicity, which correlated with increases in both lipid peroxidation and DNA damage. Furthermore, exposure at low dose rate did not appear to reduce the ability of UVA to induce the phenomenon of persistent genomic instability. Pretreatment with the antioxidant vitamin E significantly protected against UVA dose-rate effects observed with respect to lipid peroxidation and survival. Additionally, cell populations irradiated at low dose rate exhibited a shift towards a more pro-oxidant state. Taken together, these observations suggest an oxidative stress mechanism is underlying the UVA dose-rate effect. 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However, the effect of altering the intensity or dose rate of UV, which varies considerably with geographical location, the time of day or year, and the use of sunscreens, is not understood. In this study, the effect of altering the dose rate of UVA was investigated in the immortalized human keratinocyte cell line, HaCaT. Lowering the dose rate of UVA resulted in increased cytotoxicity, which correlated with increases in both lipid peroxidation and DNA damage. Furthermore, exposure at low dose rate did not appear to reduce the ability of UVA to induce the phenomenon of persistent genomic instability. Pretreatment with the antioxidant vitamin E significantly protected against UVA dose-rate effects observed with respect to lipid peroxidation and survival. Additionally, cell populations irradiated at low dose rate exhibited a shift towards a more pro-oxidant state. Taken together, these observations suggest an oxidative stress mechanism is underlying the UVA dose-rate effect. 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| subjects | Antioxidants - pharmacology Biological and medical sciences Cell Line, Transformed Cell Survival - drug effects Cell Survival - radiation effects Dermatology Dose-Response Relationship, Radiation Genomic Instability - radiation effects Humans Keratinocytes - cytology Keratinocytes - metabolism Keratinocytes - radiation effects Lipid Peroxidation - drug effects Lipid Peroxidation - radiation effects Medical sciences Micronucleus Tests Oxidative Stress - drug effects Oxidative Stress - radiation effects Reactive Oxygen Species - metabolism Ultraviolet Rays Vitamin E - pharmacology |
| title | The Dose Rate of UVA Treatment Influences the Cellular Response of HaCaT Keratinocytes |
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