Root-end Filling Materials Alter Fibroblast Differentiation
Root-end filling materials are commonly used following endodontic surgical procedures; however, their effect on adjacent soft tissues is poorly understood. We predict that, due to the differences in their chemical composition, these materials will have profoundly different effects on the survival an...
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| Veröffentlicht in: | Journal of dental research 2004-05, Vol.83 (5), p.408-413 |
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| creator | Bonson, S. Jeansonne, B.G. Lallier, T.E. |
| description | Root-end filling materials are commonly used following endodontic surgical procedures; however, their effect on adjacent soft tissues is poorly understood. We predict that, due to the differences in their chemical composition, these materials will have profoundly different effects on the survival and differentiation of fibroblasts. Many of the root-end filling materials examined were initially cytotoxic to both PDL and gingival fibroblasts in co-culture experiments; however, this was reduced after the materials were washed in either mineral trioxide aggregate (MTA) or hybrid ionomere composite resin (HICR) for 2 wks. Additionally, PDL fibroblasts displayed enhanced proliferation on MTA and survival on amalgam when compared with gingival fibroblasts. MTA preferentially induced alkaline phosphatase expression and activity in both PDL and gingival fibroblasts. In contrast, HICR inhibited alkaline phosphatase expression and activity. In addition, MTA and HICR repressed pleiotrophin in PDL fibroblasts, while HICR repressed periostin in both fibroblasts. Thus, root-end filling materials differentially affect periodontal fibroblast differentiation. Abbreviations: mineral trioxide aggregate (MTA), zinc-oxide eugenol cement (ZOEC), hybrid ionomer composite resin (HICR), reverse-transcriptase polymerase chain-reaction (RT-PCR). |
| doi_str_mv | 10.1177/154405910408300511 |
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We predict that, due to the differences in their chemical composition, these materials will have profoundly different effects on the survival and differentiation of fibroblasts. Many of the root-end filling materials examined were initially cytotoxic to both PDL and gingival fibroblasts in co-culture experiments; however, this was reduced after the materials were washed in either mineral trioxide aggregate (MTA) or hybrid ionomere composite resin (HICR) for 2 wks. Additionally, PDL fibroblasts displayed enhanced proliferation on MTA and survival on amalgam when compared with gingival fibroblasts. MTA preferentially induced alkaline phosphatase expression and activity in both PDL and gingival fibroblasts. In contrast, HICR inhibited alkaline phosphatase expression and activity. In addition, MTA and HICR repressed pleiotrophin in PDL fibroblasts, while HICR repressed periostin in both fibroblasts. Thus, root-end filling materials differentially affect periodontal fibroblast differentiation. Abbreviations: mineral trioxide aggregate (MTA), zinc-oxide eugenol cement (ZOEC), hybrid ionomer composite resin (HICR), reverse-transcriptase polymerase chain-reaction (RT-PCR).</description><identifier>ISSN: 0022-0345</identifier><identifier>EISSN: 1544-0591</identifier><identifier>DOI: 10.1177/154405910408300511</identifier><identifier>PMID: 15111634</identifier><identifier>CODEN: JDREAF</identifier><language>eng</language><publisher>United States: SAGE Publications</publisher><subject>Alkaline Phosphatase - analysis ; Aluminum Compounds - toxicity ; Biomarkers - analysis ; Calcium Compounds - toxicity ; Carrier Proteins - analysis ; Cell Adhesion Molecules - analysis ; Cell Differentiation - drug effects ; Cell Division - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; Cytokines - analysis ; Dental Alloys - chemistry ; Dental Amalgam - chemistry ; Dentin-Bonding Agents - chemistry ; Drug Combinations ; Fibroblasts - drug effects ; Gingiva - cytology ; Gingiva - drug effects ; Glass Ionomer Cements - toxicity ; Growth Substances - analysis ; Humans ; Oxides - toxicity ; Periodontal Ligament - cytology ; Periodontal Ligament - drug effects ; Resins, Synthetic - toxicity ; Retrograde Obturation ; Root Canal Filling Materials - toxicity ; Silicates - toxicity ; Zinc Oxide-Eugenol Cement - toxicity</subject><ispartof>Journal of dental research, 2004-05, Vol.83 (5), p.408-413</ispartof><rights>International and American Associations for Dental Research</rights><rights>Copyright American Association for Dental Research/American Academy of Implant Dentistry May 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-246bbeab0ddbdc1e4e5f5801573aebdd5ad59f7a84c8d8c49a19aa6134fd723f3</citedby><cites>FETCH-LOGICAL-c461t-246bbeab0ddbdc1e4e5f5801573aebdd5ad59f7a84c8d8c49a19aa6134fd723f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/154405910408300511$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/154405910408300511$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15111634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bonson, S.</creatorcontrib><creatorcontrib>Jeansonne, B.G.</creatorcontrib><creatorcontrib>Lallier, T.E.</creatorcontrib><title>Root-end Filling Materials Alter Fibroblast Differentiation</title><title>Journal of dental research</title><addtitle>J Dent Res</addtitle><description>Root-end filling materials are commonly used following endodontic surgical procedures; however, their effect on adjacent soft tissues is poorly understood. We predict that, due to the differences in their chemical composition, these materials will have profoundly different effects on the survival and differentiation of fibroblasts. Many of the root-end filling materials examined were initially cytotoxic to both PDL and gingival fibroblasts in co-culture experiments; however, this was reduced after the materials were washed in either mineral trioxide aggregate (MTA) or hybrid ionomere composite resin (HICR) for 2 wks. Additionally, PDL fibroblasts displayed enhanced proliferation on MTA and survival on amalgam when compared with gingival fibroblasts. MTA preferentially induced alkaline phosphatase expression and activity in both PDL and gingival fibroblasts. In contrast, HICR inhibited alkaline phosphatase expression and activity. In addition, MTA and HICR repressed pleiotrophin in PDL fibroblasts, while HICR repressed periostin in both fibroblasts. Thus, root-end filling materials differentially affect periodontal fibroblast differentiation. Abbreviations: mineral trioxide aggregate (MTA), zinc-oxide eugenol cement (ZOEC), hybrid ionomer composite resin (HICR), reverse-transcriptase polymerase chain-reaction (RT-PCR).</description><subject>Alkaline Phosphatase - analysis</subject><subject>Aluminum Compounds - toxicity</subject><subject>Biomarkers - analysis</subject><subject>Calcium Compounds - toxicity</subject><subject>Carrier Proteins - analysis</subject><subject>Cell Adhesion Molecules - analysis</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Cytokines - analysis</subject><subject>Dental Alloys - chemistry</subject><subject>Dental Amalgam - chemistry</subject><subject>Dentin-Bonding Agents - chemistry</subject><subject>Drug Combinations</subject><subject>Fibroblasts - drug effects</subject><subject>Gingiva - cytology</subject><subject>Gingiva - drug effects</subject><subject>Glass Ionomer Cements - toxicity</subject><subject>Growth Substances - analysis</subject><subject>Humans</subject><subject>Oxides - toxicity</subject><subject>Periodontal Ligament - cytology</subject><subject>Periodontal Ligament - drug effects</subject><subject>Resins, Synthetic - toxicity</subject><subject>Retrograde Obturation</subject><subject>Root Canal Filling Materials - toxicity</subject><subject>Silicates - toxicity</subject><subject>Zinc Oxide-Eugenol Cement - toxicity</subject><issn>0022-0345</issn><issn>1544-0591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kEtLAzEUhYMotlb_gAsZ3I-9N4954KqoVaEiiK6HZJKUlOlMTdKF_96UFroQXN3L4TvnwCHkGuEOsSynKDgHUSNwqBiAQDwh452Y79RTMgagNAfGxYhchLACwJpW7JyMMLFYMD4m9x_DEHPT62zuus71y-xNRuOd7EI269KXdOUH1ckQs0dnrfGmj05GN_SX5Mwmzlwd7oR8zZ8-H17yxfvz68Nskbe8wJhTXihlpAKtlW7RcCOsqABFyaRRWgupRW1LWfG20lXLa4m1lAUybnVJmWUTcrvP3fjhe2tCbFbD1vepsqFQc440wRNC91DrhxC8sc3Gu7X0Pw1Cs5ur-TtXMt0ckrdqbfTRctgnAdM9EOTSHGv_ifwFMeRx8A</recordid><startdate>20040501</startdate><enddate>20040501</enddate><creator>Bonson, S.</creator><creator>Jeansonne, B.G.</creator><creator>Lallier, T.E.</creator><general>SAGE Publications</general><general>SAGE PUBLICATIONS, INC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RQ</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>U9A</scope></search><sort><creationdate>20040501</creationdate><title>Root-end Filling Materials Alter Fibroblast Differentiation</title><author>Bonson, S. ; Jeansonne, B.G. ; Lallier, T.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-246bbeab0ddbdc1e4e5f5801573aebdd5ad59f7a84c8d8c49a19aa6134fd723f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Alkaline Phosphatase - analysis</topic><topic>Aluminum Compounds - toxicity</topic><topic>Biomarkers - analysis</topic><topic>Calcium Compounds - toxicity</topic><topic>Carrier Proteins - analysis</topic><topic>Cell Adhesion Molecules - analysis</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Division - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Cytokines - analysis</topic><topic>Dental Alloys - chemistry</topic><topic>Dental Amalgam - chemistry</topic><topic>Dentin-Bonding Agents - chemistry</topic><topic>Drug Combinations</topic><topic>Fibroblasts - drug effects</topic><topic>Gingiva - cytology</topic><topic>Gingiva - drug effects</topic><topic>Glass Ionomer Cements - toxicity</topic><topic>Growth Substances - analysis</topic><topic>Humans</topic><topic>Oxides - toxicity</topic><topic>Periodontal Ligament - cytology</topic><topic>Periodontal Ligament - drug effects</topic><topic>Resins, Synthetic - toxicity</topic><topic>Retrograde Obturation</topic><topic>Root Canal Filling Materials - toxicity</topic><topic>Silicates - toxicity</topic><topic>Zinc Oxide-Eugenol Cement - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bonson, S.</creatorcontrib><creatorcontrib>Jeansonne, B.G.</creatorcontrib><creatorcontrib>Lallier, T.E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Career & Technical Education Database</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><jtitle>Journal of dental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bonson, S.</au><au>Jeansonne, B.G.</au><au>Lallier, T.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Root-end Filling Materials Alter Fibroblast Differentiation</atitle><jtitle>Journal of dental research</jtitle><addtitle>J Dent Res</addtitle><date>2004-05-01</date><risdate>2004</risdate><volume>83</volume><issue>5</issue><spage>408</spage><epage>413</epage><pages>408-413</pages><issn>0022-0345</issn><eissn>1544-0591</eissn><coden>JDREAF</coden><abstract>Root-end filling materials are commonly used following endodontic surgical procedures; however, their effect on adjacent soft tissues is poorly understood. We predict that, due to the differences in their chemical composition, these materials will have profoundly different effects on the survival and differentiation of fibroblasts. Many of the root-end filling materials examined were initially cytotoxic to both PDL and gingival fibroblasts in co-culture experiments; however, this was reduced after the materials were washed in either mineral trioxide aggregate (MTA) or hybrid ionomere composite resin (HICR) for 2 wks. Additionally, PDL fibroblasts displayed enhanced proliferation on MTA and survival on amalgam when compared with gingival fibroblasts. MTA preferentially induced alkaline phosphatase expression and activity in both PDL and gingival fibroblasts. In contrast, HICR inhibited alkaline phosphatase expression and activity. In addition, MTA and HICR repressed pleiotrophin in PDL fibroblasts, while HICR repressed periostin in both fibroblasts. Thus, root-end filling materials differentially affect periodontal fibroblast differentiation. Abbreviations: mineral trioxide aggregate (MTA), zinc-oxide eugenol cement (ZOEC), hybrid ionomer composite resin (HICR), reverse-transcriptase polymerase chain-reaction (RT-PCR).</abstract><cop>United States</cop><pub>SAGE Publications</pub><pmid>15111634</pmid><doi>10.1177/154405910408300511</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0022-0345 |
| ispartof | Journal of dental research, 2004-05, Vol.83 (5), p.408-413 |
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| language | eng |
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| subjects | Alkaline Phosphatase - analysis Aluminum Compounds - toxicity Biomarkers - analysis Calcium Compounds - toxicity Carrier Proteins - analysis Cell Adhesion Molecules - analysis Cell Differentiation - drug effects Cell Division - drug effects Cell Survival - drug effects Cells, Cultured Cytokines - analysis Dental Alloys - chemistry Dental Amalgam - chemistry Dentin-Bonding Agents - chemistry Drug Combinations Fibroblasts - drug effects Gingiva - cytology Gingiva - drug effects Glass Ionomer Cements - toxicity Growth Substances - analysis Humans Oxides - toxicity Periodontal Ligament - cytology Periodontal Ligament - drug effects Resins, Synthetic - toxicity Retrograde Obturation Root Canal Filling Materials - toxicity Silicates - toxicity Zinc Oxide-Eugenol Cement - toxicity |
| title | Root-end Filling Materials Alter Fibroblast Differentiation |
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