Spectrophotometric determination of ligand–protein ratio in B2M3–DTPA monoclonal conjugates based on yttrium–methylthymol blue complex
Monoclonal conjugates using radionuclides are widely used in the diagnosis or therapy of cancer. A number of ligand molecules per protein molecule (L/P ratio) are a crucial factor affecting the monoclonal conjugate efficacy and safety. For proper and accurate dosing of monoclonal conjugates, it is n...
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| Veröffentlicht in: | Monatshefte für Chemie 2018-09, Vol.149 (9), p.1653-1657 |
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| creator | Nesměrák, Karel Třmínková-Němcová, Pavlína Drož, Ladislav Goliáš, Jaroslav Jedelský, Petr |
| description | Monoclonal conjugates using radionuclides are widely used in the diagnosis or therapy of cancer. A number of ligand molecules per protein molecule (L/P ratio) are a crucial factor affecting the monoclonal conjugate efficacy and safety. For proper and accurate dosing of monoclonal conjugates, it is necessary to establish the L/P ratio before they are applied. The aim of this work was the development of a simple, efficient, non-radioactive, and low-cost spectrophotometric method for L/P ratio measurement. The method is based on transchelation reaction between the monoclonal conjugate and yttrium(III)–methylthymol blue complex followed at wavelength of 590 nm. The method operates in the L/P ratio range of 0.32–8.00 and the results are satisfactorily comparable to the conventional mass spectrometric method.
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| doi_str_mv | 10.1007/s00706-018-2248-1 |
| format | Article |
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| subjects | Analytical Chemistry Chemistry Chemistry and Materials Science Chemistry/Food Science Conjugates Inorganic Chemistry Ligands Monoclonal antibodies Organic Chemistry Original Paper Physical Chemistry Proteins Radioisotopes Spectrometry Spectrophotometry Theoretical and Computational Chemistry Yttrium |
| title | Spectrophotometric determination of ligand–protein ratio in B2M3–DTPA monoclonal conjugates based on yttrium–methylthymol blue complex |
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