A New Form-Factor Method for the Analysis of Tissue Fluorescence

The aim of this article is to present a developed method that decomposes the autofluorescence spectrum into the spectra of naturally occurring biochemical components of biotissue. It requires knowledge of detailed spectrum behaviour of different endogenous fluorophores. We have studied the main bio-markers in human tissue and proposed a simple modelling algorithm for their spectra shapes. The empirical method was tested theoretically by quantum-mechanical calculations of the spectra in the unharmonic Morse potential approach.