Pseudomonas diversity in western Algeria: role in the stimulation of bean germination and common bean blight biocontrol
The aim of this work was to determine the functional diversity of soil bacteria belonging to the Pseudomonas genus, to study their effects on bean ( Phaseollus vulgaris L) seed germination and their biocontrol potential of common bean blight. Bacteria were isolated and identified based on physiologi...
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| Veröffentlicht in: | European journal of plant pathology 2019-02, Vol.153 (2), p.397-415 |
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| creator | Mokrani, Slimane Rai, Abdelwahab Belabid, Lakhder Cherif, Ameur Cherif, Hanane Mahjoubi, Mouna Nabti, Elhafid |
| description | The aim of this work was to determine the functional diversity of soil bacteria belonging to the
Pseudomonas
genus, to study their effects on bean (
Phaseollus vulgaris
L) seed germination and their biocontrol potential of common bean blight. Bacteria were isolated and identified based on physiological and biochemical characters and BOX-PCR. Followed by qualitative and/or quantitative analysis of their secondary metabolites. 50 soil bacteria were affected to the two groups of fluorescent (72%) and non-fluorescent Pseudomonads (28%). The UPGMA (Unweighted Pair Group Method with Arithmetic Mean) showed five phenons of carbon sources assimilation; at the time that BOX-PCR profiling resulted in five clusters characterized by 29 different haplotypes. (66%) isolates induced phosphate solubilization; (24%) were HCN producers, (21%) showed IAA production and all isolates had produced siderophores. In vitro antibacterial activity against Xapf showed 26.67 and 24 mm of inhibition zone using the two isolates
P. grimontii
P25 and
P. cepatia
P7
,
respectively. Similarly
,
the same isolates significantly reduced Xapf-bean common blight intensity, while their co-inoculation was less effective. The isolate
P. cepatia
P7 was highly effective on seed germination and root growth properties, then
P. grimontii
P25. Thus, the selected isolates could play a crucial bean growth promotion and bean common blight biocontrol as alternative to chemicals for crop yield enhancement. |
| doi_str_mv | 10.1007/s10658-018-1566-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2089010249</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2089010249</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-7eb41de492914198080ad393baeda99d66c583a1208e4bc44a163a436c7197c83</originalsourceid><addsrcrecordid>eNp1kE1LAzEQhoMoWD9-gLeA59XMJs0m3krxCwp60HPI7qZtym5Sk6yl_94sK3jyNDDzPu_MvAjdALkDQqr7CITPRUFAFDDnvJAnaAbzihaCcXmKZkSWsgBR0XN0EeOOZEbKcoYO79EMre-90xG39tuEaNMRW4cPJiYTHF50GxOsfsDBd2YcpK3BMdl-6HSy3mG_xrXRDmdZb93U067Fje-z7TSrO7vZJlxb33iXstMVOlvrLprr33qJPp8eP5Yvxert-XW5WBUNBZ6KytQMWsPy9cBACiKIbqmktTatlrLlvJkLqqEkwrC6YUwDp5pR3lQgq0bQS3Q7-e6D_xryS2rnh-DySpUZSYCUTGYVTKom-BiDWat9sL0ORwVEjfmqKV-V81VjvmpkyomJWevy83_O_0M_6h5--w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2089010249</pqid></control><display><type>article</type><title>Pseudomonas diversity in western Algeria: role in the stimulation of bean germination and common bean blight biocontrol</title><source>SpringerLink Journals</source><creator>Mokrani, Slimane ; Rai, Abdelwahab ; Belabid, Lakhder ; Cherif, Ameur ; Cherif, Hanane ; Mahjoubi, Mouna ; Nabti, Elhafid</creator><creatorcontrib>Mokrani, Slimane ; Rai, Abdelwahab ; Belabid, Lakhder ; Cherif, Ameur ; Cherif, Hanane ; Mahjoubi, Mouna ; Nabti, Elhafid</creatorcontrib><description>The aim of this work was to determine the functional diversity of soil bacteria belonging to the
Pseudomonas
genus, to study their effects on bean (
Phaseollus vulgaris
L) seed germination and their biocontrol potential of common bean blight. Bacteria were isolated and identified based on physiological and biochemical characters and BOX-PCR. Followed by qualitative and/or quantitative analysis of their secondary metabolites. 50 soil bacteria were affected to the two groups of fluorescent (72%) and non-fluorescent Pseudomonads (28%). The UPGMA (Unweighted Pair Group Method with Arithmetic Mean) showed five phenons of carbon sources assimilation; at the time that BOX-PCR profiling resulted in five clusters characterized by 29 different haplotypes. (66%) isolates induced phosphate solubilization; (24%) were HCN producers, (21%) showed IAA production and all isolates had produced siderophores. In vitro antibacterial activity against Xapf showed 26.67 and 24 mm of inhibition zone using the two isolates
P. grimontii
P25 and
P. cepatia
P7
,
respectively. Similarly
,
the same isolates significantly reduced Xapf-bean common blight intensity, while their co-inoculation was less effective. The isolate
P. cepatia
P7 was highly effective on seed germination and root growth properties, then
P. grimontii
P25. Thus, the selected isolates could play a crucial bean growth promotion and bean common blight biocontrol as alternative to chemicals for crop yield enhancement.</description><identifier>ISSN: 0929-1873</identifier><identifier>EISSN: 1573-8469</identifier><identifier>DOI: 10.1007/s10658-018-1566-9</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agriculture ; Antibacterial activity ; Bacteria ; Biological control ; Biomedical and Life Sciences ; Blight ; Carbon sources ; Crop yield ; Ecology ; Fluorescence ; Germination ; Haplotypes ; Inoculation ; Life Sciences ; Metabolites ; Organic chemistry ; Phaseolus vulgaris ; Plant growth ; Plant Pathology ; Plant Sciences ; Pseudomonas ; Qualitative analysis ; Quantitative analysis ; Secondary metabolites ; Seed germination ; Siderophores ; Soil bacteria ; Soil microorganisms ; Solubilization</subject><ispartof>European journal of plant pathology, 2019-02, Vol.153 (2), p.397-415</ispartof><rights>Koninklijke Nederlandse Planteziektenkundige Vereniging 2018</rights><rights>European Journal of Plant Pathology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-7eb41de492914198080ad393baeda99d66c583a1208e4bc44a163a436c7197c83</citedby><cites>FETCH-LOGICAL-c316t-7eb41de492914198080ad393baeda99d66c583a1208e4bc44a163a436c7197c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10658-018-1566-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10658-018-1566-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Mokrani, Slimane</creatorcontrib><creatorcontrib>Rai, Abdelwahab</creatorcontrib><creatorcontrib>Belabid, Lakhder</creatorcontrib><creatorcontrib>Cherif, Ameur</creatorcontrib><creatorcontrib>Cherif, Hanane</creatorcontrib><creatorcontrib>Mahjoubi, Mouna</creatorcontrib><creatorcontrib>Nabti, Elhafid</creatorcontrib><title>Pseudomonas diversity in western Algeria: role in the stimulation of bean germination and common bean blight biocontrol</title><title>European journal of plant pathology</title><addtitle>Eur J Plant Pathol</addtitle><description>The aim of this work was to determine the functional diversity of soil bacteria belonging to the
Pseudomonas
genus, to study their effects on bean (
Phaseollus vulgaris
L) seed germination and their biocontrol potential of common bean blight. Bacteria were isolated and identified based on physiological and biochemical characters and BOX-PCR. Followed by qualitative and/or quantitative analysis of their secondary metabolites. 50 soil bacteria were affected to the two groups of fluorescent (72%) and non-fluorescent Pseudomonads (28%). The UPGMA (Unweighted Pair Group Method with Arithmetic Mean) showed five phenons of carbon sources assimilation; at the time that BOX-PCR profiling resulted in five clusters characterized by 29 different haplotypes. (66%) isolates induced phosphate solubilization; (24%) were HCN producers, (21%) showed IAA production and all isolates had produced siderophores. In vitro antibacterial activity against Xapf showed 26.67 and 24 mm of inhibition zone using the two isolates
P. grimontii
P25 and
P. cepatia
P7
,
respectively. Similarly
,
the same isolates significantly reduced Xapf-bean common blight intensity, while their co-inoculation was less effective. The isolate
P. cepatia
P7 was highly effective on seed germination and root growth properties, then
P. grimontii
P25. Thus, the selected isolates could play a crucial bean growth promotion and bean common blight biocontrol as alternative to chemicals for crop yield enhancement.</description><subject>Agriculture</subject><subject>Antibacterial activity</subject><subject>Bacteria</subject><subject>Biological control</subject><subject>Biomedical and Life Sciences</subject><subject>Blight</subject><subject>Carbon sources</subject><subject>Crop yield</subject><subject>Ecology</subject><subject>Fluorescence</subject><subject>Germination</subject><subject>Haplotypes</subject><subject>Inoculation</subject><subject>Life Sciences</subject><subject>Metabolites</subject><subject>Organic chemistry</subject><subject>Phaseolus vulgaris</subject><subject>Plant growth</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Pseudomonas</subject><subject>Qualitative analysis</subject><subject>Quantitative analysis</subject><subject>Secondary metabolites</subject><subject>Seed germination</subject><subject>Siderophores</subject><subject>Soil bacteria</subject><subject>Soil microorganisms</subject><subject>Solubilization</subject><issn>0929-1873</issn><issn>1573-8469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kE1LAzEQhoMoWD9-gLeA59XMJs0m3krxCwp60HPI7qZtym5Sk6yl_94sK3jyNDDzPu_MvAjdALkDQqr7CITPRUFAFDDnvJAnaAbzihaCcXmKZkSWsgBR0XN0EeOOZEbKcoYO79EMre-90xG39tuEaNMRW4cPJiYTHF50GxOsfsDBd2YcpK3BMdl-6HSy3mG_xrXRDmdZb93U067Fje-z7TSrO7vZJlxb33iXstMVOlvrLprr33qJPp8eP5Yvxert-XW5WBUNBZ6KytQMWsPy9cBACiKIbqmktTatlrLlvJkLqqEkwrC6YUwDp5pR3lQgq0bQS3Q7-e6D_xryS2rnh-DySpUZSYCUTGYVTKom-BiDWat9sL0ORwVEjfmqKV-V81VjvmpkyomJWevy83_O_0M_6h5--w</recordid><startdate>20190201</startdate><enddate>20190201</enddate><creator>Mokrani, Slimane</creator><creator>Rai, Abdelwahab</creator><creator>Belabid, Lakhder</creator><creator>Cherif, Ameur</creator><creator>Cherif, Hanane</creator><creator>Mahjoubi, Mouna</creator><creator>Nabti, Elhafid</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X2</scope><scope>88A</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20190201</creationdate><title>Pseudomonas diversity in western Algeria: role in the stimulation of bean germination and common bean blight biocontrol</title><author>Mokrani, Slimane ; Rai, Abdelwahab ; Belabid, Lakhder ; Cherif, Ameur ; Cherif, Hanane ; Mahjoubi, Mouna ; Nabti, Elhafid</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-7eb41de492914198080ad393baeda99d66c583a1208e4bc44a163a436c7197c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Agriculture</topic><topic>Antibacterial activity</topic><topic>Bacteria</topic><topic>Biological control</topic><topic>Biomedical and Life Sciences</topic><topic>Blight</topic><topic>Carbon sources</topic><topic>Crop yield</topic><topic>Ecology</topic><topic>Fluorescence</topic><topic>Germination</topic><topic>Haplotypes</topic><topic>Inoculation</topic><topic>Life Sciences</topic><topic>Metabolites</topic><topic>Organic chemistry</topic><topic>Phaseolus vulgaris</topic><topic>Plant growth</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Pseudomonas</topic><topic>Qualitative analysis</topic><topic>Quantitative analysis</topic><topic>Secondary metabolites</topic><topic>Seed germination</topic><topic>Siderophores</topic><topic>Soil bacteria</topic><topic>Soil microorganisms</topic><topic>Solubilization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mokrani, Slimane</creatorcontrib><creatorcontrib>Rai, Abdelwahab</creatorcontrib><creatorcontrib>Belabid, Lakhder</creatorcontrib><creatorcontrib>Cherif, Ameur</creatorcontrib><creatorcontrib>Cherif, Hanane</creatorcontrib><creatorcontrib>Mahjoubi, Mouna</creatorcontrib><creatorcontrib>Nabti, Elhafid</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>European journal of plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mokrani, Slimane</au><au>Rai, Abdelwahab</au><au>Belabid, Lakhder</au><au>Cherif, Ameur</au><au>Cherif, Hanane</au><au>Mahjoubi, Mouna</au><au>Nabti, Elhafid</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pseudomonas diversity in western Algeria: role in the stimulation of bean germination and common bean blight biocontrol</atitle><jtitle>European journal of plant pathology</jtitle><stitle>Eur J Plant Pathol</stitle><date>2019-02-01</date><risdate>2019</risdate><volume>153</volume><issue>2</issue><spage>397</spage><epage>415</epage><pages>397-415</pages><issn>0929-1873</issn><eissn>1573-8469</eissn><abstract>The aim of this work was to determine the functional diversity of soil bacteria belonging to the
Pseudomonas
genus, to study their effects on bean (
Phaseollus vulgaris
L) seed germination and their biocontrol potential of common bean blight. Bacteria were isolated and identified based on physiological and biochemical characters and BOX-PCR. Followed by qualitative and/or quantitative analysis of their secondary metabolites. 50 soil bacteria were affected to the two groups of fluorescent (72%) and non-fluorescent Pseudomonads (28%). The UPGMA (Unweighted Pair Group Method with Arithmetic Mean) showed five phenons of carbon sources assimilation; at the time that BOX-PCR profiling resulted in five clusters characterized by 29 different haplotypes. (66%) isolates induced phosphate solubilization; (24%) were HCN producers, (21%) showed IAA production and all isolates had produced siderophores. In vitro antibacterial activity against Xapf showed 26.67 and 24 mm of inhibition zone using the two isolates
P. grimontii
P25 and
P. cepatia
P7
,
respectively. Similarly
,
the same isolates significantly reduced Xapf-bean common blight intensity, while their co-inoculation was less effective. The isolate
P. cepatia
P7 was highly effective on seed germination and root growth properties, then
P. grimontii
P25. Thus, the selected isolates could play a crucial bean growth promotion and bean common blight biocontrol as alternative to chemicals for crop yield enhancement.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10658-018-1566-9</doi><tpages>19</tpages></addata></record> |
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| ispartof | European journal of plant pathology, 2019-02, Vol.153 (2), p.397-415 |
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| source | SpringerLink Journals |
| subjects | Agriculture Antibacterial activity Bacteria Biological control Biomedical and Life Sciences Blight Carbon sources Crop yield Ecology Fluorescence Germination Haplotypes Inoculation Life Sciences Metabolites Organic chemistry Phaseolus vulgaris Plant growth Plant Pathology Plant Sciences Pseudomonas Qualitative analysis Quantitative analysis Secondary metabolites Seed germination Siderophores Soil bacteria Soil microorganisms Solubilization |
| title | Pseudomonas diversity in western Algeria: role in the stimulation of bean germination and common bean blight biocontrol |
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