Fusaric acid is a virulence factor of Fusarium oxysporum f. sp. cubense on banana plantlets
Fusaric acid (FA) is highly phytotoxic and causes severe Fusarium oxysporum -induced vascular wilt. However, the molecular mechanisms of FA production in Fusarium oxysporum f. sp. cubense (FOC) are largely unknown. In this study, FoFUB4 , a F. verticillioides FUB4 homolog was cloned from banana path...
Gespeichert in:
| Veröffentlicht in: | Tropical Plant Pathology 2018-08, Vol.43 (4), p.297-305 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 305 |
|---|---|
| container_issue | 4 |
| container_start_page | 297 |
| container_title | Tropical Plant Pathology |
| container_volume | 43 |
| creator | Ding, Zhaojian Yang, Laying Wang, Guofen Guo, Lijia Liu, Lei Wang, Jun Huang, Junsheng |
| description | Fusaric acid (FA) is highly phytotoxic and causes severe
Fusarium oxysporum
-induced vascular wilt. However, the molecular mechanisms of FA production in
Fusarium oxysporum
f. sp.
cubense
(FOC) are largely unknown. In this study,
FoFUB4
, a
F. verticillioides FUB4
homolog was cloned from banana pathogenic fungus FOC and its deletion mutants were generated using homologous recombination. Our null mutation results showed that
FoFUB4
did not affect fungal development and conidiation, but was essential for virulence on banana plantlets compared with wild type (WT) strain. Biochemical analysis showed that production of FA was not detected in mutant Δ
FoFUB4
and FA production by WT was significantly reduced in Czapek Dox medium containing 6 compared with 120 mM NaNO
3
. Moreover, transcriptional analysis indicated that the expression levels of
FoFUB4
were reduced in WT in Czapek Dox medium containing 6 compared with 120 mM NaNO
3
. Taken together, these results provide direct evidence for the vital roles of FA in virulence, and that nitrogen source regulated FA biosynthesis in FOC. |
| doi_str_mv | 10.1007/s40858-018-0230-4 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_journals_2088015803</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A707407231</galeid><sourcerecordid>A707407231</sourcerecordid><originalsourceid>FETCH-LOGICAL-c398t-7652764c28011c087d23a88097655f4dc8bf029be8a9fc3841b5982ecd47b8473</originalsourceid><addsrcrecordid>eNp1kEFLxDAQhYMouK7-AG8Bz62TNG3S47K4Kix40ZOHkKbJ0qWb1KQV99-bpYJeZAgZkve9GR5CtwRyAsDvIwNRigxIOrSAjJ2hBalFkVEo6fmf_hJdxbgHqGhdiQV630xRhU5jpbsWdxEr_NmFqTdOG2yVHn3A3uJZNR2w_zrGwYfU2RzHIcd6aoyLBnuHG-VS4aFXbuzNGK_RhVV9NDc_9xK9bR5e10_Z9uXxeb3aZrqoxZjxqqS8YpoKIESD4C0tlBBQp4_SslaLxgKtGyNUbXUhGGnKWlCjW8YbwXixRHez7xD8x2TiKPd-Ci6NlBSSESkFFEmVz6qd6o3snPVjUDpVaw6d9s7YLr2vOHAGnBYkAWQGdPAxBmPlELqDCkdJQJ5Cl3PoMoUuT6FLlhg6MzFp3c6E31X-h74BjWqCzw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2088015803</pqid></control><display><type>article</type><title>Fusaric acid is a virulence factor of Fusarium oxysporum f. sp. cubense on banana plantlets</title><source>SpringerLink Journals - AutoHoldings</source><creator>Ding, Zhaojian ; Yang, Laying ; Wang, Guofen ; Guo, Lijia ; Liu, Lei ; Wang, Jun ; Huang, Junsheng</creator><creatorcontrib>Ding, Zhaojian ; Yang, Laying ; Wang, Guofen ; Guo, Lijia ; Liu, Lei ; Wang, Jun ; Huang, Junsheng</creatorcontrib><description>Fusaric acid (FA) is highly phytotoxic and causes severe
Fusarium oxysporum
-induced vascular wilt. However, the molecular mechanisms of FA production in
Fusarium oxysporum
f. sp.
cubense
(FOC) are largely unknown. In this study,
FoFUB4
, a
F. verticillioides FUB4
homolog was cloned from banana pathogenic fungus FOC and its deletion mutants were generated using homologous recombination. Our null mutation results showed that
FoFUB4
did not affect fungal development and conidiation, but was essential for virulence on banana plantlets compared with wild type (WT) strain. Biochemical analysis showed that production of FA was not detected in mutant Δ
FoFUB4
and FA production by WT was significantly reduced in Czapek Dox medium containing 6 compared with 120 mM NaNO
3
. Moreover, transcriptional analysis indicated that the expression levels of
FoFUB4
were reduced in WT in Czapek Dox medium containing 6 compared with 120 mM NaNO
3
. Taken together, these results provide direct evidence for the vital roles of FA in virulence, and that nitrogen source regulated FA biosynthesis in FOC.</description><identifier>ISSN: 1983-2052</identifier><identifier>ISSN: 1982-5676</identifier><identifier>EISSN: 1983-2052</identifier><identifier>DOI: 10.1007/s40858-018-0230-4</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Biochemical analysis ; Biomedical and Life Sciences ; Biosynthesis ; Clonal deletion ; Deletion mutant ; Fungi ; Fusaric acid ; Fusarium ; Fusarium oxysporum ; Gene deletion ; Homologous recombination ; Homology ; Life Sciences ; Molecular modelling ; Musaceae ; Mutants ; Original Article ; Plant Pathology ; Plantlets ; Transcription ; Virulence ; Virulence factors ; Wilt</subject><ispartof>Tropical Plant Pathology, 2018-08, Vol.43 (4), p.297-305</ispartof><rights>Sociedade Brasileira de Fitopatologia 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Copyright Springer Nature B.V. 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-7652764c28011c087d23a88097655f4dc8bf029be8a9fc3841b5982ecd47b8473</citedby><cites>FETCH-LOGICAL-c398t-7652764c28011c087d23a88097655f4dc8bf029be8a9fc3841b5982ecd47b8473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s40858-018-0230-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s40858-018-0230-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27915,27916,41479,42548,51310</link.rule.ids></links><search><creatorcontrib>Ding, Zhaojian</creatorcontrib><creatorcontrib>Yang, Laying</creatorcontrib><creatorcontrib>Wang, Guofen</creatorcontrib><creatorcontrib>Guo, Lijia</creatorcontrib><creatorcontrib>Liu, Lei</creatorcontrib><creatorcontrib>Wang, Jun</creatorcontrib><creatorcontrib>Huang, Junsheng</creatorcontrib><title>Fusaric acid is a virulence factor of Fusarium oxysporum f. sp. cubense on banana plantlets</title><title>Tropical Plant Pathology</title><addtitle>Trop. plant pathol</addtitle><description>Fusaric acid (FA) is highly phytotoxic and causes severe
Fusarium oxysporum
-induced vascular wilt. However, the molecular mechanisms of FA production in
Fusarium oxysporum
f. sp.
cubense
(FOC) are largely unknown. In this study,
FoFUB4
, a
F. verticillioides FUB4
homolog was cloned from banana pathogenic fungus FOC and its deletion mutants were generated using homologous recombination. Our null mutation results showed that
FoFUB4
did not affect fungal development and conidiation, but was essential for virulence on banana plantlets compared with wild type (WT) strain. Biochemical analysis showed that production of FA was not detected in mutant Δ
FoFUB4
and FA production by WT was significantly reduced in Czapek Dox medium containing 6 compared with 120 mM NaNO
3
. Moreover, transcriptional analysis indicated that the expression levels of
FoFUB4
were reduced in WT in Czapek Dox medium containing 6 compared with 120 mM NaNO
3
. Taken together, these results provide direct evidence for the vital roles of FA in virulence, and that nitrogen source regulated FA biosynthesis in FOC.</description><subject>Biochemical analysis</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Clonal deletion</subject><subject>Deletion mutant</subject><subject>Fungi</subject><subject>Fusaric acid</subject><subject>Fusarium</subject><subject>Fusarium oxysporum</subject><subject>Gene deletion</subject><subject>Homologous recombination</subject><subject>Homology</subject><subject>Life Sciences</subject><subject>Molecular modelling</subject><subject>Musaceae</subject><subject>Mutants</subject><subject>Original Article</subject><subject>Plant Pathology</subject><subject>Plantlets</subject><subject>Transcription</subject><subject>Virulence</subject><subject>Virulence factors</subject><subject>Wilt</subject><issn>1983-2052</issn><issn>1982-5676</issn><issn>1983-2052</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kEFLxDAQhYMouK7-AG8Bz62TNG3S47K4Kix40ZOHkKbJ0qWb1KQV99-bpYJeZAgZkve9GR5CtwRyAsDvIwNRigxIOrSAjJ2hBalFkVEo6fmf_hJdxbgHqGhdiQV630xRhU5jpbsWdxEr_NmFqTdOG2yVHn3A3uJZNR2w_zrGwYfU2RzHIcd6aoyLBnuHG-VS4aFXbuzNGK_RhVV9NDc_9xK9bR5e10_Z9uXxeb3aZrqoxZjxqqS8YpoKIESD4C0tlBBQp4_SslaLxgKtGyNUbXUhGGnKWlCjW8YbwXixRHez7xD8x2TiKPd-Ci6NlBSSESkFFEmVz6qd6o3snPVjUDpVaw6d9s7YLr2vOHAGnBYkAWQGdPAxBmPlELqDCkdJQJ5Cl3PoMoUuT6FLlhg6MzFp3c6E31X-h74BjWqCzw</recordid><startdate>20180801</startdate><enddate>20180801</enddate><creator>Ding, Zhaojian</creator><creator>Yang, Laying</creator><creator>Wang, Guofen</creator><creator>Guo, Lijia</creator><creator>Liu, Lei</creator><creator>Wang, Jun</creator><creator>Huang, Junsheng</creator><general>Springer International Publishing</general><general>Springer</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>IAO</scope></search><sort><creationdate>20180801</creationdate><title>Fusaric acid is a virulence factor of Fusarium oxysporum f. sp. cubense on banana plantlets</title><author>Ding, Zhaojian ; Yang, Laying ; Wang, Guofen ; Guo, Lijia ; Liu, Lei ; Wang, Jun ; Huang, Junsheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-7652764c28011c087d23a88097655f4dc8bf029be8a9fc3841b5982ecd47b8473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Biochemical analysis</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Clonal deletion</topic><topic>Deletion mutant</topic><topic>Fungi</topic><topic>Fusaric acid</topic><topic>Fusarium</topic><topic>Fusarium oxysporum</topic><topic>Gene deletion</topic><topic>Homologous recombination</topic><topic>Homology</topic><topic>Life Sciences</topic><topic>Molecular modelling</topic><topic>Musaceae</topic><topic>Mutants</topic><topic>Original Article</topic><topic>Plant Pathology</topic><topic>Plantlets</topic><topic>Transcription</topic><topic>Virulence</topic><topic>Virulence factors</topic><topic>Wilt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ding, Zhaojian</creatorcontrib><creatorcontrib>Yang, Laying</creatorcontrib><creatorcontrib>Wang, Guofen</creatorcontrib><creatorcontrib>Guo, Lijia</creatorcontrib><creatorcontrib>Liu, Lei</creatorcontrib><creatorcontrib>Wang, Jun</creatorcontrib><creatorcontrib>Huang, Junsheng</creatorcontrib><collection>CrossRef</collection><collection>Gale Academic OneFile</collection><jtitle>Tropical Plant Pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ding, Zhaojian</au><au>Yang, Laying</au><au>Wang, Guofen</au><au>Guo, Lijia</au><au>Liu, Lei</au><au>Wang, Jun</au><au>Huang, Junsheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fusaric acid is a virulence factor of Fusarium oxysporum f. sp. cubense on banana plantlets</atitle><jtitle>Tropical Plant Pathology</jtitle><stitle>Trop. plant pathol</stitle><date>2018-08-01</date><risdate>2018</risdate><volume>43</volume><issue>4</issue><spage>297</spage><epage>305</epage><pages>297-305</pages><issn>1983-2052</issn><issn>1982-5676</issn><eissn>1983-2052</eissn><abstract>Fusaric acid (FA) is highly phytotoxic and causes severe
Fusarium oxysporum
-induced vascular wilt. However, the molecular mechanisms of FA production in
Fusarium oxysporum
f. sp.
cubense
(FOC) are largely unknown. In this study,
FoFUB4
, a
F. verticillioides FUB4
homolog was cloned from banana pathogenic fungus FOC and its deletion mutants were generated using homologous recombination. Our null mutation results showed that
FoFUB4
did not affect fungal development and conidiation, but was essential for virulence on banana plantlets compared with wild type (WT) strain. Biochemical analysis showed that production of FA was not detected in mutant Δ
FoFUB4
and FA production by WT was significantly reduced in Czapek Dox medium containing 6 compared with 120 mM NaNO
3
. Moreover, transcriptional analysis indicated that the expression levels of
FoFUB4
were reduced in WT in Czapek Dox medium containing 6 compared with 120 mM NaNO
3
. Taken together, these results provide direct evidence for the vital roles of FA in virulence, and that nitrogen source regulated FA biosynthesis in FOC.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s40858-018-0230-4</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1983-2052 |
| ispartof | Tropical Plant Pathology, 2018-08, Vol.43 (4), p.297-305 |
| issn | 1983-2052 1982-5676 1983-2052 |
| language | eng |
| recordid | cdi_proquest_journals_2088015803 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Biochemical analysis Biomedical and Life Sciences Biosynthesis Clonal deletion Deletion mutant Fungi Fusaric acid Fusarium Fusarium oxysporum Gene deletion Homologous recombination Homology Life Sciences Molecular modelling Musaceae Mutants Original Article Plant Pathology Plantlets Transcription Virulence Virulence factors Wilt |
| title | Fusaric acid is a virulence factor of Fusarium oxysporum f. sp. cubense on banana plantlets |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T00%3A25%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Fusaric%20acid%20is%20a%20virulence%20factor%20of%20Fusarium%20oxysporum%20f.%20sp.%20cubense%20on%20banana%20plantlets&rft.jtitle=Tropical%20Plant%20Pathology&rft.au=Ding,%20Zhaojian&rft.date=2018-08-01&rft.volume=43&rft.issue=4&rft.spage=297&rft.epage=305&rft.pages=297-305&rft.issn=1983-2052&rft.eissn=1983-2052&rft_id=info:doi/10.1007/s40858-018-0230-4&rft_dat=%3Cgale_proqu%3EA707407231%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2088015803&rft_id=info:pmid/&rft_galeid=A707407231&rfr_iscdi=true |