Exposure to the insecticides permethrin and malathion induces leukemia and lymphoma-associated gene aberrations in vitro
Epidemiological studies have associated the exposure to permethrin and malathion with increased risk of leukemia and lymphoma. The aim of this study was to evaluate whether in vitro exposure to permethrin and malathion induces aberrations in genes involved in the etiology of these hematological mali...
Gespeichert in:
| Veröffentlicht in: | Toxicology in vitro 2017-10, Vol.44, p.17-26 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 26 |
|---|---|
| container_issue | |
| container_start_page | 17 |
| container_title | Toxicology in vitro |
| container_volume | 44 |
| creator | Navarrete-Meneses, M.P. Salas-Labadía, C. Sanabrais-Jiménez, M. Santana-Hernández, J. Serrano-Cuevas, A. Juárez-Velázquez, R. Olaya-Vargas, A. Pérez-Vera, P. |
| description | Epidemiological studies have associated the exposure to permethrin and malathion with increased risk of leukemia and lymphoma. The aim of this study was to evaluate whether in vitro exposure to permethrin and malathion induces aberrations in genes involved in the etiology of these hematological malignancies. Genetic abnormalities in the IGH, KMT2A (MLL), ETV6 and RUNX1 genes, and aneuploidy induced by the in vitro exposure to permethrin and malathion (200μM, 24h), were analyzed by FISH in peripheral blood mononuclear cells (PBMCs). The gene fusions IGH-BCL2, KMT2A-AFF1 and ETV6-RUNX1 were further analyzed with nested RT-PCR in PBMCs, and in K562 cells exposed to acute and chronic treatments (0.1μM, 24h or every third day for two weeks) of insecticides. FISH analysis revealed that permethrin induces aneuploidy and structural alterations in IGH and KMT2A genes, and malathion induces breaks in KMT2A. RT-PCR detected ETV6-RUNX1 fusion in PBMCs acutely exposed to permethrin. Permethrin also induced ETV6-RUNX1 and IGH-BCL2 fusions in K562 cells, and malathion induced KMT2A-AFF1 and ETV6-RUNX1 fusions. Overall, we identified that both insecticides induce breaks and fusions in the studied genes, and permethrin induces aneuploidy. This study presents evidence of damage in cancer genes caused by these insecticides.
•Permethrin induces breaks in KMT2A and IGH genes in peripheral blood mononuclear cells (PBMCs) exposed in vitro.•Permethrin induces aneuploidy in PBMCs exposed in vitro.•Malathion induces KMT2A breaks in PBMCs exposed in vitro.•Permethrin and malathion induced oncogenic fusions in PBMCs and K562 cells. |
| doi_str_mv | 10.1016/j.tiv.2017.06.013 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2085135918</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0887233317301625</els_id><sourcerecordid>2085135918</sourcerecordid><originalsourceid>FETCH-LOGICAL-c381t-bb86d7d584b8d1f7318c7caa4fd991f71ea2ed6102933fdf9464ca54c7c185a13</originalsourceid><addsrcrecordid>eNp9kE1P3DAQhq2Kqrt8_IBekCXOST1xEjviVCEKSEi9tGfLsSest5s42M4K_n29LPTYkzXy876jeQj5CqwEBu23bZncvqwYiJK1JQP-iaxBiq7gIMQJWTMpRVFxzlfkNMYtY6yRFftCVpVsq7oW9Zq83L7MPi4BafI0bZC6KaJJzjiLkc4YRkyb4CaqJ0tHvdNp4_yUKbuYDOxw-YOj02_fu9dx3vhRFzpGb5xOaOkTTkh1jyHolIMxJ-nepeDPyedB7yJevL9n5PeP218398Xjz7uHm--PheESUtH3srXCNrLupYVBcJBGGK3rwXZdngF1hbYFVnWcD3bo6rY2uqkzBLLRwM_I1bF3Dv55wZjU1i9hyitVxWQDvOlAZgqOlAk-xoCDmoMbdXhVwNTBtdqq7FodXCvWquw6Zy7fm5d-RPsv8SE3A9dHAPN9e4dBReNwMmhdyI6V9e4_9X8B6GOSDg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2085135918</pqid></control><display><type>article</type><title>Exposure to the insecticides permethrin and malathion induces leukemia and lymphoma-associated gene aberrations in vitro</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Navarrete-Meneses, M.P. ; Salas-Labadía, C. ; Sanabrais-Jiménez, M. ; Santana-Hernández, J. ; Serrano-Cuevas, A. ; Juárez-Velázquez, R. ; Olaya-Vargas, A. ; Pérez-Vera, P.</creator><creatorcontrib>Navarrete-Meneses, M.P. ; Salas-Labadía, C. ; Sanabrais-Jiménez, M. ; Santana-Hernández, J. ; Serrano-Cuevas, A. ; Juárez-Velázquez, R. ; Olaya-Vargas, A. ; Pérez-Vera, P.</creatorcontrib><description>Epidemiological studies have associated the exposure to permethrin and malathion with increased risk of leukemia and lymphoma. The aim of this study was to evaluate whether in vitro exposure to permethrin and malathion induces aberrations in genes involved in the etiology of these hematological malignancies. Genetic abnormalities in the IGH, KMT2A (MLL), ETV6 and RUNX1 genes, and aneuploidy induced by the in vitro exposure to permethrin and malathion (200μM, 24h), were analyzed by FISH in peripheral blood mononuclear cells (PBMCs). The gene fusions IGH-BCL2, KMT2A-AFF1 and ETV6-RUNX1 were further analyzed with nested RT-PCR in PBMCs, and in K562 cells exposed to acute and chronic treatments (0.1μM, 24h or every third day for two weeks) of insecticides. FISH analysis revealed that permethrin induces aneuploidy and structural alterations in IGH and KMT2A genes, and malathion induces breaks in KMT2A. RT-PCR detected ETV6-RUNX1 fusion in PBMCs acutely exposed to permethrin. Permethrin also induced ETV6-RUNX1 and IGH-BCL2 fusions in K562 cells, and malathion induced KMT2A-AFF1 and ETV6-RUNX1 fusions. Overall, we identified that both insecticides induce breaks and fusions in the studied genes, and permethrin induces aneuploidy. This study presents evidence of damage in cancer genes caused by these insecticides.
•Permethrin induces breaks in KMT2A and IGH genes in peripheral blood mononuclear cells (PBMCs) exposed in vitro.•Permethrin induces aneuploidy in PBMCs exposed in vitro.•Malathion induces KMT2A breaks in PBMCs exposed in vitro.•Permethrin and malathion induced oncogenic fusions in PBMCs and K562 cells.</description><identifier>ISSN: 0887-2333</identifier><identifier>EISSN: 1879-3177</identifier><identifier>DOI: 10.1016/j.tiv.2017.06.013</identifier><identifier>PMID: 28624474</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Abnormalities ; Aneuploidy ; Cancer ; Cell Survival - drug effects ; Cells, Cultured ; Epidemiology ; Etiology ; Exposure ; Gene Fusion ; Genes ; Genetic abnormalities ; Heavy chains ; Hematology ; Humans ; IGH ; Immunoglobulins ; Insecticides ; Insecticides - toxicity ; K562 Cells ; KMT2A ; Leukemia ; Leukemia - genetics ; Leukocytes (mononuclear) ; Leukocytes, Mononuclear - drug effects ; Leukocytes, Mononuclear - metabolism ; Lymphoma ; Lymphoma - genetics ; Malathion ; Malathion - toxicity ; Male ; MLL ; Oncogene Proteins, Fusion - genetics ; Peripheral blood mononuclear cells ; Permethrin ; Permethrin - toxicity ; Polymerase chain reaction ; Runx1 protein</subject><ispartof>Toxicology in vitro, 2017-10, Vol.44, p.17-26</ispartof><rights>2017 Elsevier Ltd</rights><rights>Copyright © 2017 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. Oct 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-bb86d7d584b8d1f7318c7caa4fd991f71ea2ed6102933fdf9464ca54c7c185a13</citedby><cites>FETCH-LOGICAL-c381t-bb86d7d584b8d1f7318c7caa4fd991f71ea2ed6102933fdf9464ca54c7c185a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.tiv.2017.06.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28624474$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Navarrete-Meneses, M.P.</creatorcontrib><creatorcontrib>Salas-Labadía, C.</creatorcontrib><creatorcontrib>Sanabrais-Jiménez, M.</creatorcontrib><creatorcontrib>Santana-Hernández, J.</creatorcontrib><creatorcontrib>Serrano-Cuevas, A.</creatorcontrib><creatorcontrib>Juárez-Velázquez, R.</creatorcontrib><creatorcontrib>Olaya-Vargas, A.</creatorcontrib><creatorcontrib>Pérez-Vera, P.</creatorcontrib><title>Exposure to the insecticides permethrin and malathion induces leukemia and lymphoma-associated gene aberrations in vitro</title><title>Toxicology in vitro</title><addtitle>Toxicol In Vitro</addtitle><description>Epidemiological studies have associated the exposure to permethrin and malathion with increased risk of leukemia and lymphoma. The aim of this study was to evaluate whether in vitro exposure to permethrin and malathion induces aberrations in genes involved in the etiology of these hematological malignancies. Genetic abnormalities in the IGH, KMT2A (MLL), ETV6 and RUNX1 genes, and aneuploidy induced by the in vitro exposure to permethrin and malathion (200μM, 24h), were analyzed by FISH in peripheral blood mononuclear cells (PBMCs). The gene fusions IGH-BCL2, KMT2A-AFF1 and ETV6-RUNX1 were further analyzed with nested RT-PCR in PBMCs, and in K562 cells exposed to acute and chronic treatments (0.1μM, 24h or every third day for two weeks) of insecticides. FISH analysis revealed that permethrin induces aneuploidy and structural alterations in IGH and KMT2A genes, and malathion induces breaks in KMT2A. RT-PCR detected ETV6-RUNX1 fusion in PBMCs acutely exposed to permethrin. Permethrin also induced ETV6-RUNX1 and IGH-BCL2 fusions in K562 cells, and malathion induced KMT2A-AFF1 and ETV6-RUNX1 fusions. Overall, we identified that both insecticides induce breaks and fusions in the studied genes, and permethrin induces aneuploidy. This study presents evidence of damage in cancer genes caused by these insecticides.
•Permethrin induces breaks in KMT2A and IGH genes in peripheral blood mononuclear cells (PBMCs) exposed in vitro.•Permethrin induces aneuploidy in PBMCs exposed in vitro.•Malathion induces KMT2A breaks in PBMCs exposed in vitro.•Permethrin and malathion induced oncogenic fusions in PBMCs and K562 cells.</description><subject>Abnormalities</subject><subject>Aneuploidy</subject><subject>Cancer</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Epidemiology</subject><subject>Etiology</subject><subject>Exposure</subject><subject>Gene Fusion</subject><subject>Genes</subject><subject>Genetic abnormalities</subject><subject>Heavy chains</subject><subject>Hematology</subject><subject>Humans</subject><subject>IGH</subject><subject>Immunoglobulins</subject><subject>Insecticides</subject><subject>Insecticides - toxicity</subject><subject>K562 Cells</subject><subject>KMT2A</subject><subject>Leukemia</subject><subject>Leukemia - genetics</subject><subject>Leukocytes (mononuclear)</subject><subject>Leukocytes, Mononuclear - drug effects</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Lymphoma</subject><subject>Lymphoma - genetics</subject><subject>Malathion</subject><subject>Malathion - toxicity</subject><subject>Male</subject><subject>MLL</subject><subject>Oncogene Proteins, Fusion - genetics</subject><subject>Peripheral blood mononuclear cells</subject><subject>Permethrin</subject><subject>Permethrin - toxicity</subject><subject>Polymerase chain reaction</subject><subject>Runx1 protein</subject><issn>0887-2333</issn><issn>1879-3177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1P3DAQhq2Kqrt8_IBekCXOST1xEjviVCEKSEi9tGfLsSest5s42M4K_n29LPTYkzXy876jeQj5CqwEBu23bZncvqwYiJK1JQP-iaxBiq7gIMQJWTMpRVFxzlfkNMYtY6yRFftCVpVsq7oW9Zq83L7MPi4BafI0bZC6KaJJzjiLkc4YRkyb4CaqJ0tHvdNp4_yUKbuYDOxw-YOj02_fu9dx3vhRFzpGb5xOaOkTTkh1jyHolIMxJ-nepeDPyedB7yJevL9n5PeP218398Xjz7uHm--PheESUtH3srXCNrLupYVBcJBGGK3rwXZdngF1hbYFVnWcD3bo6rY2uqkzBLLRwM_I1bF3Dv55wZjU1i9hyitVxWQDvOlAZgqOlAk-xoCDmoMbdXhVwNTBtdqq7FodXCvWquw6Zy7fm5d-RPsv8SE3A9dHAPN9e4dBReNwMmhdyI6V9e4_9X8B6GOSDg</recordid><startdate>201710</startdate><enddate>201710</enddate><creator>Navarrete-Meneses, M.P.</creator><creator>Salas-Labadía, C.</creator><creator>Sanabrais-Jiménez, M.</creator><creator>Santana-Hernández, J.</creator><creator>Serrano-Cuevas, A.</creator><creator>Juárez-Velázquez, R.</creator><creator>Olaya-Vargas, A.</creator><creator>Pérez-Vera, P.</creator><general>Elsevier Ltd</general><general>Elsevier Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>201710</creationdate><title>Exposure to the insecticides permethrin and malathion induces leukemia and lymphoma-associated gene aberrations in vitro</title><author>Navarrete-Meneses, M.P. ; Salas-Labadía, C. ; Sanabrais-Jiménez, M. ; Santana-Hernández, J. ; Serrano-Cuevas, A. ; Juárez-Velázquez, R. ; Olaya-Vargas, A. ; Pérez-Vera, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-bb86d7d584b8d1f7318c7caa4fd991f71ea2ed6102933fdf9464ca54c7c185a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Abnormalities</topic><topic>Aneuploidy</topic><topic>Cancer</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Epidemiology</topic><topic>Etiology</topic><topic>Exposure</topic><topic>Gene Fusion</topic><topic>Genes</topic><topic>Genetic abnormalities</topic><topic>Heavy chains</topic><topic>Hematology</topic><topic>Humans</topic><topic>IGH</topic><topic>Immunoglobulins</topic><topic>Insecticides</topic><topic>Insecticides - toxicity</topic><topic>K562 Cells</topic><topic>KMT2A</topic><topic>Leukemia</topic><topic>Leukemia - genetics</topic><topic>Leukocytes (mononuclear)</topic><topic>Leukocytes, Mononuclear - drug effects</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Lymphoma</topic><topic>Lymphoma - genetics</topic><topic>Malathion</topic><topic>Malathion - toxicity</topic><topic>Male</topic><topic>MLL</topic><topic>Oncogene Proteins, Fusion - genetics</topic><topic>Peripheral blood mononuclear cells</topic><topic>Permethrin</topic><topic>Permethrin - toxicity</topic><topic>Polymerase chain reaction</topic><topic>Runx1 protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Navarrete-Meneses, M.P.</creatorcontrib><creatorcontrib>Salas-Labadía, C.</creatorcontrib><creatorcontrib>Sanabrais-Jiménez, M.</creatorcontrib><creatorcontrib>Santana-Hernández, J.</creatorcontrib><creatorcontrib>Serrano-Cuevas, A.</creatorcontrib><creatorcontrib>Juárez-Velázquez, R.</creatorcontrib><creatorcontrib>Olaya-Vargas, A.</creatorcontrib><creatorcontrib>Pérez-Vera, P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology in vitro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Navarrete-Meneses, M.P.</au><au>Salas-Labadía, C.</au><au>Sanabrais-Jiménez, M.</au><au>Santana-Hernández, J.</au><au>Serrano-Cuevas, A.</au><au>Juárez-Velázquez, R.</au><au>Olaya-Vargas, A.</au><au>Pérez-Vera, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exposure to the insecticides permethrin and malathion induces leukemia and lymphoma-associated gene aberrations in vitro</atitle><jtitle>Toxicology in vitro</jtitle><addtitle>Toxicol In Vitro</addtitle><date>2017-10</date><risdate>2017</risdate><volume>44</volume><spage>17</spage><epage>26</epage><pages>17-26</pages><issn>0887-2333</issn><eissn>1879-3177</eissn><abstract>Epidemiological studies have associated the exposure to permethrin and malathion with increased risk of leukemia and lymphoma. The aim of this study was to evaluate whether in vitro exposure to permethrin and malathion induces aberrations in genes involved in the etiology of these hematological malignancies. Genetic abnormalities in the IGH, KMT2A (MLL), ETV6 and RUNX1 genes, and aneuploidy induced by the in vitro exposure to permethrin and malathion (200μM, 24h), were analyzed by FISH in peripheral blood mononuclear cells (PBMCs). The gene fusions IGH-BCL2, KMT2A-AFF1 and ETV6-RUNX1 were further analyzed with nested RT-PCR in PBMCs, and in K562 cells exposed to acute and chronic treatments (0.1μM, 24h or every third day for two weeks) of insecticides. FISH analysis revealed that permethrin induces aneuploidy and structural alterations in IGH and KMT2A genes, and malathion induces breaks in KMT2A. RT-PCR detected ETV6-RUNX1 fusion in PBMCs acutely exposed to permethrin. Permethrin also induced ETV6-RUNX1 and IGH-BCL2 fusions in K562 cells, and malathion induced KMT2A-AFF1 and ETV6-RUNX1 fusions. Overall, we identified that both insecticides induce breaks and fusions in the studied genes, and permethrin induces aneuploidy. This study presents evidence of damage in cancer genes caused by these insecticides.
•Permethrin induces breaks in KMT2A and IGH genes in peripheral blood mononuclear cells (PBMCs) exposed in vitro.•Permethrin induces aneuploidy in PBMCs exposed in vitro.•Malathion induces KMT2A breaks in PBMCs exposed in vitro.•Permethrin and malathion induced oncogenic fusions in PBMCs and K562 cells.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>28624474</pmid><doi>10.1016/j.tiv.2017.06.013</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0887-2333 |
| ispartof | Toxicology in vitro, 2017-10, Vol.44, p.17-26 |
| issn | 0887-2333 1879-3177 |
| language | eng |
| recordid | cdi_proquest_journals_2085135918 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Abnormalities Aneuploidy Cancer Cell Survival - drug effects Cells, Cultured Epidemiology Etiology Exposure Gene Fusion Genes Genetic abnormalities Heavy chains Hematology Humans IGH Immunoglobulins Insecticides Insecticides - toxicity K562 Cells KMT2A Leukemia Leukemia - genetics Leukocytes (mononuclear) Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - metabolism Lymphoma Lymphoma - genetics Malathion Malathion - toxicity Male MLL Oncogene Proteins, Fusion - genetics Peripheral blood mononuclear cells Permethrin Permethrin - toxicity Polymerase chain reaction Runx1 protein |
| title | Exposure to the insecticides permethrin and malathion induces leukemia and lymphoma-associated gene aberrations in vitro |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T22%3A17%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Exposure%20to%20the%20insecticides%20permethrin%20and%20malathion%20induces%20leukemia%20and%20lymphoma-associated%20gene%20aberrations%20in%20vitro&rft.jtitle=Toxicology%20in%20vitro&rft.au=Navarrete-Meneses,%20M.P.&rft.date=2017-10&rft.volume=44&rft.spage=17&rft.epage=26&rft.pages=17-26&rft.issn=0887-2333&rft.eissn=1879-3177&rft_id=info:doi/10.1016/j.tiv.2017.06.013&rft_dat=%3Cproquest_cross%3E2085135918%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2085135918&rft_id=info:pmid/28624474&rft_els_id=S0887233317301625&rfr_iscdi=true |