Testing a Nonpathogenic Surrogate Microorganism for Validating Desiccation-Adapted Salmonella Inactivation in Physically Heat-Treated Broiler Litter
Thermal resistance of desiccation-adapted Salmonella Senftenberg 775W was compared with that of Enterococcus faecium NRRL B-2354 in aged broiler litter. Aged broiler litter with 20, 30, and 40% moisture contents was inoculated separately with desiccation-adapted Salmonella Senftenberg 775W and E. fa...
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| Veröffentlicht in: | Journal of food protection 2018-09, Vol.81 (9), p.1418-1424 |
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| creator | Wang, Hongye Chen, Zhao Li, Mengzhe Greene, Annel K Jiang, Xiuping Wang, Jingxue |
| description | Thermal resistance of desiccation-adapted Salmonella Senftenberg 775W was compared with that of Enterococcus faecium NRRL B-2354 in aged broiler litter. Aged broiler litter with 20, 30, and 40% moisture contents was inoculated separately with desiccation-adapted Salmonella Senftenberg 775W and E. faecium NRRL B-2354 at ca. 5 to 6 log CFU/g and then heat treated at 75, 85, and 150°C. At all tested temperatures, desiccation-adapted E. faecium NRRL B-2354 was more heat resistant than desiccation-adapted Salmonella Senftenberg 775W ( P < 0.05). During the treatments at 75 and 85°C, E. faecium NRRL B-2354 in aged broiler litter with all moisture contents was reduced by 2.89 to 4.12 log and was above the detection limit of direct plating (1.30 log CFU/g), whereas Salmonella Senftenberg 775W could not be detected by enrichment (>5-log reduction) during holding time at these temperatures. At 150°C, E. faecium NRRL B-2354 in aged broiler litter with 20 and 30% moisture contents was still detectable by enrichment after heat exposure for up to 15 min, whereas Salmonella Senftenberg 775W in aged broiler litter with all moisture contents could not be detected throughout the entire treatment. Our results revealed that E. faecium NRRL B-2354 can be used as a surrogate for Salmonella to validate the thermal processing of poultry litter by providing a sufficient safety margin. This study provides a practical tool for poultry litter processors to evaluate the effectiveness of their thermal processing. |
| doi_str_mv | 10.4315/0362-028X.JFP-18-141 |
| format | Article |
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Aged broiler litter with 20, 30, and 40% moisture contents was inoculated separately with desiccation-adapted Salmonella Senftenberg 775W and E. faecium NRRL B-2354 at ca. 5 to 6 log CFU/g and then heat treated at 75, 85, and 150°C. At all tested temperatures, desiccation-adapted E. faecium NRRL B-2354 was more heat resistant than desiccation-adapted Salmonella Senftenberg 775W ( P < 0.05). During the treatments at 75 and 85°C, E. faecium NRRL B-2354 in aged broiler litter with all moisture contents was reduced by 2.89 to 4.12 log and was above the detection limit of direct plating (1.30 log CFU/g), whereas Salmonella Senftenberg 775W could not be detected by enrichment (>5-log reduction) during holding time at these temperatures. At 150°C, E. faecium NRRL B-2354 in aged broiler litter with 20 and 30% moisture contents was still detectable by enrichment after heat exposure for up to 15 min, whereas Salmonella Senftenberg 775W in aged broiler litter with all moisture contents could not be detected throughout the entire treatment. Our results revealed that E. faecium NRRL B-2354 can be used as a surrogate for Salmonella to validate the thermal processing of poultry litter by providing a sufficient safety margin. This study provides a practical tool for poultry litter processors to evaluate the effectiveness of their thermal processing.</description><identifier>ISSN: 0362-028X</identifier><identifier>EISSN: 1944-9097</identifier><identifier>DOI: 10.4315/0362-028X.JFP-18-141</identifier><identifier>PMID: 30059252</identifier><language>eng</language><publisher>United States: Elsevier Limited</publisher><subject>Adaptation ; Adaptation, Physiological ; Agricultural wastes ; Agriculture ; Aluminum ; Animals ; Chickens - microbiology ; Composting ; Deactivation ; Desiccation ; E coli ; Food ; Food Microbiology ; Food safety ; Heat ; Heat treating ; Heat treatment ; Hot Temperature ; Inactivation ; Litter ; Manure - microbiology ; Microorganisms ; Modernization ; Moisture ; Moisture content ; Pathogens ; Performance evaluation ; Potassium ; Poultry ; Safety margins ; Salmonella ; Salmonella - growth & development ; Salmonella - physiology ; Studies ; Temperature ; Thermal resistance</subject><ispartof>Journal of food protection, 2018-09, Vol.81 (9), p.1418-1424</ispartof><rights>Copyright Allen Press Publishing Services Sep 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-3452d40761bfe2a43c0ce0fe6969895761ed78484e7b6bdb308153f68c2b634e3</citedby><cites>FETCH-LOGICAL-c381t-3452d40761bfe2a43c0ce0fe6969895761ed78484e7b6bdb308153f68c2b634e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2084409010?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,64385,64389,72469</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30059252$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Hongye</creatorcontrib><creatorcontrib>Chen, Zhao</creatorcontrib><creatorcontrib>Li, Mengzhe</creatorcontrib><creatorcontrib>Greene, Annel K</creatorcontrib><creatorcontrib>Jiang, Xiuping</creatorcontrib><creatorcontrib>Wang, Jingxue</creatorcontrib><title>Testing a Nonpathogenic Surrogate Microorganism for Validating Desiccation-Adapted Salmonella Inactivation in Physically Heat-Treated Broiler Litter</title><title>Journal of food protection</title><addtitle>J Food Prot</addtitle><description>Thermal resistance of desiccation-adapted Salmonella Senftenberg 775W was compared with that of Enterococcus faecium NRRL B-2354 in aged broiler litter. Aged broiler litter with 20, 30, and 40% moisture contents was inoculated separately with desiccation-adapted Salmonella Senftenberg 775W and E. faecium NRRL B-2354 at ca. 5 to 6 log CFU/g and then heat treated at 75, 85, and 150°C. At all tested temperatures, desiccation-adapted E. faecium NRRL B-2354 was more heat resistant than desiccation-adapted Salmonella Senftenberg 775W ( P < 0.05). During the treatments at 75 and 85°C, E. faecium NRRL B-2354 in aged broiler litter with all moisture contents was reduced by 2.89 to 4.12 log and was above the detection limit of direct plating (1.30 log CFU/g), whereas Salmonella Senftenberg 775W could not be detected by enrichment (>5-log reduction) during holding time at these temperatures. At 150°C, E. faecium NRRL B-2354 in aged broiler litter with 20 and 30% moisture contents was still detectable by enrichment after heat exposure for up to 15 min, whereas Salmonella Senftenberg 775W in aged broiler litter with all moisture contents could not be detected throughout the entire treatment. Our results revealed that E. faecium NRRL B-2354 can be used as a surrogate for Salmonella to validate the thermal processing of poultry litter by providing a sufficient safety margin. This study provides a practical tool for poultry litter processors to evaluate the effectiveness of their thermal processing.</description><subject>Adaptation</subject><subject>Adaptation, Physiological</subject><subject>Agricultural wastes</subject><subject>Agriculture</subject><subject>Aluminum</subject><subject>Animals</subject><subject>Chickens - microbiology</subject><subject>Composting</subject><subject>Deactivation</subject><subject>Desiccation</subject><subject>E coli</subject><subject>Food</subject><subject>Food Microbiology</subject><subject>Food safety</subject><subject>Heat</subject><subject>Heat treating</subject><subject>Heat treatment</subject><subject>Hot Temperature</subject><subject>Inactivation</subject><subject>Litter</subject><subject>Manure - microbiology</subject><subject>Microorganisms</subject><subject>Modernization</subject><subject>Moisture</subject><subject>Moisture content</subject><subject>Pathogens</subject><subject>Performance evaluation</subject><subject>Potassium</subject><subject>Poultry</subject><subject>Safety margins</subject><subject>Salmonella</subject><subject>Salmonella - growth & development</subject><subject>Salmonella - physiology</subject><subject>Studies</subject><subject>Temperature</subject><subject>Thermal resistance</subject><issn>0362-028X</issn><issn>1944-9097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNo9kd1uEzEQhS0EoqHwBghZ4tpl_LMb-7IUSluFtlID4s7yemdTVxs79TpIeQ8eGKd_N7Y1PueM9B1CPnI4UpI3X0C2goHQf44uTq8Z14wr_orMuFGKGTDz12T2Ijkg76bpDgCEEe1bciABGiMaMSP_ljiVEFfU0csUN67cphXG4OnNNue0cgXpz-BzSnnlYpjWdEiZ_nZj6N2D7RtOwfv6TpEd925TsKc3blyniOPo6Hl0voS_D_80RHp9u6t6N447eoausGWuZ7V8zSmMmOkilIL5PXkzuHHCD0_3Ifl1-n15csYWVz_OT44XzEvNC5OqEb2Cecu7AYVT0oNHGLA1rdGmqXPs51pphfOu7fpOguaNHFrtRddKhfKQfH7M3eR0v60g7F3a5lhXWgFaKTDAoarUo6pimKaMg93ksHZ5ZznYfRV2z9nuOdtaheXa1iqq7dNT-LZbY_9iemYv_wPiVodN</recordid><startdate>201809</startdate><enddate>201809</enddate><creator>Wang, Hongye</creator><creator>Chen, Zhao</creator><creator>Li, Mengzhe</creator><creator>Greene, Annel K</creator><creator>Jiang, Xiuping</creator><creator>Wang, Jingxue</creator><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RQ</scope><scope>7WY</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>883</scope><scope>88E</scope><scope>88I</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>M0F</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PATMY</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope></search><sort><creationdate>201809</creationdate><title>Testing a Nonpathogenic Surrogate Microorganism for Validating Desiccation-Adapted Salmonella Inactivation in Physically Heat-Treated Broiler Litter</title><author>Wang, Hongye ; Chen, Zhao ; Li, Mengzhe ; Greene, Annel K ; Jiang, Xiuping ; Wang, Jingxue</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-3452d40761bfe2a43c0ce0fe6969895761ed78484e7b6bdb308153f68c2b634e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adaptation</topic><topic>Adaptation, Physiological</topic><topic>Agricultural wastes</topic><topic>Agriculture</topic><topic>Aluminum</topic><topic>Animals</topic><topic>Chickens - 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Aged broiler litter with 20, 30, and 40% moisture contents was inoculated separately with desiccation-adapted Salmonella Senftenberg 775W and E. faecium NRRL B-2354 at ca. 5 to 6 log CFU/g and then heat treated at 75, 85, and 150°C. At all tested temperatures, desiccation-adapted E. faecium NRRL B-2354 was more heat resistant than desiccation-adapted Salmonella Senftenberg 775W ( P < 0.05). During the treatments at 75 and 85°C, E. faecium NRRL B-2354 in aged broiler litter with all moisture contents was reduced by 2.89 to 4.12 log and was above the detection limit of direct plating (1.30 log CFU/g), whereas Salmonella Senftenberg 775W could not be detected by enrichment (>5-log reduction) during holding time at these temperatures. At 150°C, E. faecium NRRL B-2354 in aged broiler litter with 20 and 30% moisture contents was still detectable by enrichment after heat exposure for up to 15 min, whereas Salmonella Senftenberg 775W in aged broiler litter with all moisture contents could not be detected throughout the entire treatment. Our results revealed that E. faecium NRRL B-2354 can be used as a surrogate for Salmonella to validate the thermal processing of poultry litter by providing a sufficient safety margin. This study provides a practical tool for poultry litter processors to evaluate the effectiveness of their thermal processing.</abstract><cop>United States</cop><pub>Elsevier Limited</pub><pmid>30059252</pmid><doi>10.4315/0362-028X.JFP-18-141</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adaptation Adaptation, Physiological Agricultural wastes Agriculture Aluminum Animals Chickens - microbiology Composting Deactivation Desiccation E coli Food Food Microbiology Food safety Heat Heat treating Heat treatment Hot Temperature Inactivation Litter Manure - microbiology Microorganisms Modernization Moisture Moisture content Pathogens Performance evaluation Potassium Poultry Safety margins Salmonella Salmonella - growth & development Salmonella - physiology Studies Temperature Thermal resistance |
| title | Testing a Nonpathogenic Surrogate Microorganism for Validating Desiccation-Adapted Salmonella Inactivation in Physically Heat-Treated Broiler Litter |
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