Rapid, Stoichiometric, Site-Specific Modification of Aldehyde-Containing Proteins Using a Tandem Knoevenagel-Intra Michael Addition Reaction

Rapid, Stoichiometric, Site-Specific Modification of Aldehyde-Containing Proteins Using a Tandem Knoevenagel-Intra Michael Addition Reaction

A site-specific modification of aldehyde-containing proteins using a tandem Knoevenagel-intra Michael addition reaction was developed. The reaction featured fast kinetics (50 M–1 s–1) and favorable stoichiometry. Various functionalities could be introduced into the protein with little impact on its...

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Veröffentlicht in:Bioconjugate chemistry 2018-04, Vol.29 (4), p.1016-1020
Hauptverfasser: Yu, Jian, Shen, Da, Zhang, Hanjie, Yin, Zheng
Format: Artikel
Sprache:eng
Schlagworte:
Aldehydes
Binding sites
Cellular biology
Chemical reactions
Kinetics
Michael reaction
Proteins
Reaction kinetics
Stoichiometry
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Zusammenfassung:A site-specific modification of aldehyde-containing proteins using a tandem Knoevenagel-intra Michael addition reaction was developed. The reaction featured fast kinetics (50 M–1 s–1) and favorable stoichiometry. Various functionalities could be introduced into the protein with little impact on its function and conformation. The reaction was successfully applied in the labeling of living cells.
ISSN:1043-1802
1520-4812
DOI:10.1021/acs.bioconjchem.8b00086