Carbon Monoxide Reversibly Alters Iron Homeostasis and Respiratory Epithelial Cell Function

The dissociation of iron from heme is a major factor in iron metabolism and the cellular concentrations of the metal correlate with heme degradation. We tested the hypotheses that (1) exposure to a product of heme catabolism, carbon monoxide (CO), alters iron homeostasis in the lung and in cultured...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:American journal of respiratory cell and molecular biology 2008-06, Vol.38 (6), p.715-723
Hauptverfasser: Ghio, Andrew J, Stonehuerner, Jacqueline G, Dailey, Lisa A, Richards, Judy H, Madden, Michael D, Deng, Zhongping, Nguyen, N.-B, Callaghan, Kimberly D, Yang, Funmei, Piantadosi, Claude A
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 723
container_issue 6
container_start_page 715
container_title American journal of respiratory cell and molecular biology
container_volume 38
creator Ghio, Andrew J
Stonehuerner, Jacqueline G
Dailey, Lisa A
Richards, Judy H
Madden, Michael D
Deng, Zhongping
Nguyen, N.-B
Callaghan, Kimberly D
Yang, Funmei
Piantadosi, Claude A
description The dissociation of iron from heme is a major factor in iron metabolism and the cellular concentrations of the metal correlate with heme degradation. We tested the hypotheses that (1) exposure to a product of heme catabolism, carbon monoxide (CO), alters iron homeostasis in the lung and in cultured respiratory epithelial cells; (2) this response includes both decreased uptake and increased release of cell metal; and (3) the effects of CO on cell function track changes in metal homeostasis. In rats exposed to 50 ppm CO for 24 hours, non-heme iron concentrations decreased in the lung and increased in the liver. In respiratory epithelial cells cultured at air-liquid interface, CO exposure decreased cell non-heme iron and ferritin concentrations within 2 hours and the effect was fully reversible. CO significantly depressed iron uptake by epithelial cells, despite increased expression of divalent metal transporter-1, while iron release was elevated. The loss of non-heme iron after CO reduced cellular oxidative stress, blocked the release of the proinflammatory mediator (interleukin-8), and interfered with cell cycle protein expression. We conclude that CO reduces the iron content of the lung through both the metal uptake and release mechanisms. This loss of cellular iron after CO is in line with certain biological effects of the gas that have been implicated in the protection of cell viability.
doi_str_mv 10.1165/rcmb.2007-0179OC
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_207621529</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1484185011</sourcerecordid><originalsourceid>FETCH-LOGICAL-c357t-538c4dd6157981fbbc50069dc028a2d8417c9c437f4ecdce55feb6789d8f62543</originalsourceid><addsrcrecordid>eNpFkU1LAzEQhoMoWj_unmTx5GVrkk02ybEsfhQUQfTkIWSTrE3JbmqyVfvvTWnB0wzMM-_AMwBcIjhFqKa3UfftFEPISoiYeGkOwATRipZEcHGYe0hIiSgRJ-A0pSWECHOEjsEJ4hhWgpEJ-GhUbMNQPIch_Dpji1f7bWNyrd8UMz_mtpjHPH8MvQ1pVMmlQg0mY2nlohpD3BR3KzcurHfKF431vrhfD3p0YTgHR53yyV7s6xl4v797ax7Lp5eHeTN7KnVF2VjSimtiTI0oExx1basphLUwGmKusOEEMS00qVhHrDbaUtrZtmZcGN7VmJLqDFzvclcxfK1tGuUyrOOQT0oMWY0RxSJDcAfpGFKKtpOr6HoVNxJBuZUptzLlVqbcycwrV_vcddtb87-wt5eBmx2wcJ-LHxetTL3yPuNIquU2r-Kyliz_5A8FyH9g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>207621529</pqid></control><display><type>article</type><title>Carbon Monoxide Reversibly Alters Iron Homeostasis and Respiratory Epithelial Cell Function</title><source>MEDLINE</source><source>Journals@Ovid Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Ghio, Andrew J ; Stonehuerner, Jacqueline G ; Dailey, Lisa A ; Richards, Judy H ; Madden, Michael D ; Deng, Zhongping ; Nguyen, N.-B ; Callaghan, Kimberly D ; Yang, Funmei ; Piantadosi, Claude A</creator><creatorcontrib>Ghio, Andrew J ; Stonehuerner, Jacqueline G ; Dailey, Lisa A ; Richards, Judy H ; Madden, Michael D ; Deng, Zhongping ; Nguyen, N.-B ; Callaghan, Kimberly D ; Yang, Funmei ; Piantadosi, Claude A</creatorcontrib><description>The dissociation of iron from heme is a major factor in iron metabolism and the cellular concentrations of the metal correlate with heme degradation. We tested the hypotheses that (1) exposure to a product of heme catabolism, carbon monoxide (CO), alters iron homeostasis in the lung and in cultured respiratory epithelial cells; (2) this response includes both decreased uptake and increased release of cell metal; and (3) the effects of CO on cell function track changes in metal homeostasis. In rats exposed to 50 ppm CO for 24 hours, non-heme iron concentrations decreased in the lung and increased in the liver. In respiratory epithelial cells cultured at air-liquid interface, CO exposure decreased cell non-heme iron and ferritin concentrations within 2 hours and the effect was fully reversible. CO significantly depressed iron uptake by epithelial cells, despite increased expression of divalent metal transporter-1, while iron release was elevated. The loss of non-heme iron after CO reduced cellular oxidative stress, blocked the release of the proinflammatory mediator (interleukin-8), and interfered with cell cycle protein expression. We conclude that CO reduces the iron content of the lung through both the metal uptake and release mechanisms. This loss of cellular iron after CO is in line with certain biological effects of the gas that have been implicated in the protection of cell viability.</description><identifier>ISSN: 1044-1549</identifier><identifier>EISSN: 1535-4989</identifier><identifier>DOI: 10.1165/rcmb.2007-0179OC</identifier><identifier>PMID: 18203974</identifier><identifier>CODEN: AJRBEL</identifier><language>eng</language><publisher>United States: Am Thoracic Soc</publisher><subject>Acetaldehyde - metabolism ; Animals ; Antimetabolites - metabolism ; Antimetabolites - pharmacology ; Antioxidants - metabolism ; Bronchoalveolar Lavage ; Carbon Monoxide - metabolism ; Carbon Monoxide - pharmacology ; Cell Line ; Cell Proliferation ; Child ; Deferoxamine - metabolism ; Epithelial Cells - cytology ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Ferritins - metabolism ; Heme - chemistry ; Heme - metabolism ; Homeostasis ; Humans ; Interleukin-8 - metabolism ; Iron - metabolism ; Male ; Oxidative Stress ; Rats ; Rats, Sprague-Dawley ; Respiratory Mucosa - cytology ; Respiratory Mucosa - drug effects ; Siderophores - metabolism</subject><ispartof>American journal of respiratory cell and molecular biology, 2008-06, Vol.38 (6), p.715-723</ispartof><rights>Copyright American Thoracic Society Jun 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-538c4dd6157981fbbc50069dc028a2d8417c9c437f4ecdce55feb6789d8f62543</citedby><cites>FETCH-LOGICAL-c357t-538c4dd6157981fbbc50069dc028a2d8417c9c437f4ecdce55feb6789d8f62543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18203974$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ghio, Andrew J</creatorcontrib><creatorcontrib>Stonehuerner, Jacqueline G</creatorcontrib><creatorcontrib>Dailey, Lisa A</creatorcontrib><creatorcontrib>Richards, Judy H</creatorcontrib><creatorcontrib>Madden, Michael D</creatorcontrib><creatorcontrib>Deng, Zhongping</creatorcontrib><creatorcontrib>Nguyen, N.-B</creatorcontrib><creatorcontrib>Callaghan, Kimberly D</creatorcontrib><creatorcontrib>Yang, Funmei</creatorcontrib><creatorcontrib>Piantadosi, Claude A</creatorcontrib><title>Carbon Monoxide Reversibly Alters Iron Homeostasis and Respiratory Epithelial Cell Function</title><title>American journal of respiratory cell and molecular biology</title><addtitle>Am J Respir Cell Mol Biol</addtitle><description>The dissociation of iron from heme is a major factor in iron metabolism and the cellular concentrations of the metal correlate with heme degradation. We tested the hypotheses that (1) exposure to a product of heme catabolism, carbon monoxide (CO), alters iron homeostasis in the lung and in cultured respiratory epithelial cells; (2) this response includes both decreased uptake and increased release of cell metal; and (3) the effects of CO on cell function track changes in metal homeostasis. In rats exposed to 50 ppm CO for 24 hours, non-heme iron concentrations decreased in the lung and increased in the liver. In respiratory epithelial cells cultured at air-liquid interface, CO exposure decreased cell non-heme iron and ferritin concentrations within 2 hours and the effect was fully reversible. CO significantly depressed iron uptake by epithelial cells, despite increased expression of divalent metal transporter-1, while iron release was elevated. The loss of non-heme iron after CO reduced cellular oxidative stress, blocked the release of the proinflammatory mediator (interleukin-8), and interfered with cell cycle protein expression. We conclude that CO reduces the iron content of the lung through both the metal uptake and release mechanisms. This loss of cellular iron after CO is in line with certain biological effects of the gas that have been implicated in the protection of cell viability.</description><subject>Acetaldehyde - metabolism</subject><subject>Animals</subject><subject>Antimetabolites - metabolism</subject><subject>Antimetabolites - pharmacology</subject><subject>Antioxidants - metabolism</subject><subject>Bronchoalveolar Lavage</subject><subject>Carbon Monoxide - metabolism</subject><subject>Carbon Monoxide - pharmacology</subject><subject>Cell Line</subject><subject>Cell Proliferation</subject><subject>Child</subject><subject>Deferoxamine - metabolism</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>Ferritins - metabolism</subject><subject>Heme - chemistry</subject><subject>Heme - metabolism</subject><subject>Homeostasis</subject><subject>Humans</subject><subject>Interleukin-8 - metabolism</subject><subject>Iron - metabolism</subject><subject>Male</subject><subject>Oxidative Stress</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Respiratory Mucosa - cytology</subject><subject>Respiratory Mucosa - drug effects</subject><subject>Siderophores - metabolism</subject><issn>1044-1549</issn><issn>1535-4989</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpFkU1LAzEQhoMoWj_unmTx5GVrkk02ybEsfhQUQfTkIWSTrE3JbmqyVfvvTWnB0wzMM-_AMwBcIjhFqKa3UfftFEPISoiYeGkOwATRipZEcHGYe0hIiSgRJ-A0pSWECHOEjsEJ4hhWgpEJ-GhUbMNQPIch_Dpji1f7bWNyrd8UMz_mtpjHPH8MvQ1pVMmlQg0mY2nlohpD3BR3KzcurHfKF431vrhfD3p0YTgHR53yyV7s6xl4v797ax7Lp5eHeTN7KnVF2VjSimtiTI0oExx1basphLUwGmKusOEEMS00qVhHrDbaUtrZtmZcGN7VmJLqDFzvclcxfK1tGuUyrOOQT0oMWY0RxSJDcAfpGFKKtpOr6HoVNxJBuZUptzLlVqbcycwrV_vcddtb87-wt5eBmx2wcJ-LHxetTL3yPuNIquU2r-Kyliz_5A8FyH9g</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Ghio, Andrew J</creator><creator>Stonehuerner, Jacqueline G</creator><creator>Dailey, Lisa A</creator><creator>Richards, Judy H</creator><creator>Madden, Michael D</creator><creator>Deng, Zhongping</creator><creator>Nguyen, N.-B</creator><creator>Callaghan, Kimberly D</creator><creator>Yang, Funmei</creator><creator>Piantadosi, Claude A</creator><general>Am Thoracic Soc</general><general>American Thoracic Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope></search><sort><creationdate>20080601</creationdate><title>Carbon Monoxide Reversibly Alters Iron Homeostasis and Respiratory Epithelial Cell Function</title><author>Ghio, Andrew J ; Stonehuerner, Jacqueline G ; Dailey, Lisa A ; Richards, Judy H ; Madden, Michael D ; Deng, Zhongping ; Nguyen, N.-B ; Callaghan, Kimberly D ; Yang, Funmei ; Piantadosi, Claude A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-538c4dd6157981fbbc50069dc028a2d8417c9c437f4ecdce55feb6789d8f62543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acetaldehyde - metabolism</topic><topic>Animals</topic><topic>Antimetabolites - metabolism</topic><topic>Antimetabolites - pharmacology</topic><topic>Antioxidants - metabolism</topic><topic>Bronchoalveolar Lavage</topic><topic>Carbon Monoxide - metabolism</topic><topic>Carbon Monoxide - pharmacology</topic><topic>Cell Line</topic><topic>Cell Proliferation</topic><topic>Child</topic><topic>Deferoxamine - metabolism</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>Ferritins - metabolism</topic><topic>Heme - chemistry</topic><topic>Heme - metabolism</topic><topic>Homeostasis</topic><topic>Humans</topic><topic>Interleukin-8 - metabolism</topic><topic>Iron - metabolism</topic><topic>Male</topic><topic>Oxidative Stress</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Respiratory Mucosa - cytology</topic><topic>Respiratory Mucosa - drug effects</topic><topic>Siderophores - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ghio, Andrew J</creatorcontrib><creatorcontrib>Stonehuerner, Jacqueline G</creatorcontrib><creatorcontrib>Dailey, Lisa A</creatorcontrib><creatorcontrib>Richards, Judy H</creatorcontrib><creatorcontrib>Madden, Michael D</creatorcontrib><creatorcontrib>Deng, Zhongping</creatorcontrib><creatorcontrib>Nguyen, N.-B</creatorcontrib><creatorcontrib>Callaghan, Kimberly D</creatorcontrib><creatorcontrib>Yang, Funmei</creatorcontrib><creatorcontrib>Piantadosi, Claude A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><jtitle>American journal of respiratory cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ghio, Andrew J</au><au>Stonehuerner, Jacqueline G</au><au>Dailey, Lisa A</au><au>Richards, Judy H</au><au>Madden, Michael D</au><au>Deng, Zhongping</au><au>Nguyen, N.-B</au><au>Callaghan, Kimberly D</au><au>Yang, Funmei</au><au>Piantadosi, Claude A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carbon Monoxide Reversibly Alters Iron Homeostasis and Respiratory Epithelial Cell Function</atitle><jtitle>American journal of respiratory cell and molecular biology</jtitle><addtitle>Am J Respir Cell Mol Biol</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>38</volume><issue>6</issue><spage>715</spage><epage>723</epage><pages>715-723</pages><issn>1044-1549</issn><eissn>1535-4989</eissn><coden>AJRBEL</coden><abstract>The dissociation of iron from heme is a major factor in iron metabolism and the cellular concentrations of the metal correlate with heme degradation. We tested the hypotheses that (1) exposure to a product of heme catabolism, carbon monoxide (CO), alters iron homeostasis in the lung and in cultured respiratory epithelial cells; (2) this response includes both decreased uptake and increased release of cell metal; and (3) the effects of CO on cell function track changes in metal homeostasis. In rats exposed to 50 ppm CO for 24 hours, non-heme iron concentrations decreased in the lung and increased in the liver. In respiratory epithelial cells cultured at air-liquid interface, CO exposure decreased cell non-heme iron and ferritin concentrations within 2 hours and the effect was fully reversible. CO significantly depressed iron uptake by epithelial cells, despite increased expression of divalent metal transporter-1, while iron release was elevated. The loss of non-heme iron after CO reduced cellular oxidative stress, blocked the release of the proinflammatory mediator (interleukin-8), and interfered with cell cycle protein expression. We conclude that CO reduces the iron content of the lung through both the metal uptake and release mechanisms. This loss of cellular iron after CO is in line with certain biological effects of the gas that have been implicated in the protection of cell viability.</abstract><cop>United States</cop><pub>Am Thoracic Soc</pub><pmid>18203974</pmid><doi>10.1165/rcmb.2007-0179OC</doi><tpages>9</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1044-1549
ispartof American journal of respiratory cell and molecular biology, 2008-06, Vol.38 (6), p.715-723
issn 1044-1549
1535-4989
language eng
recordid cdi_proquest_journals_207621529
source MEDLINE; Journals@Ovid Complete; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Acetaldehyde - metabolism
Animals
Antimetabolites - metabolism
Antimetabolites - pharmacology
Antioxidants - metabolism
Bronchoalveolar Lavage
Carbon Monoxide - metabolism
Carbon Monoxide - pharmacology
Cell Line
Cell Proliferation
Child
Deferoxamine - metabolism
Epithelial Cells - cytology
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Ferritins - metabolism
Heme - chemistry
Heme - metabolism
Homeostasis
Humans
Interleukin-8 - metabolism
Iron - metabolism
Male
Oxidative Stress
Rats
Rats, Sprague-Dawley
Respiratory Mucosa - cytology
Respiratory Mucosa - drug effects
Siderophores - metabolism
title Carbon Monoxide Reversibly Alters Iron Homeostasis and Respiratory Epithelial Cell Function
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T22%3A49%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Carbon%20Monoxide%20Reversibly%20Alters%20Iron%20Homeostasis%20and%20Respiratory%20Epithelial%20Cell%20Function&rft.jtitle=American%20journal%20of%20respiratory%20cell%20and%20molecular%20biology&rft.au=Ghio,%20Andrew%20J&rft.date=2008-06-01&rft.volume=38&rft.issue=6&rft.spage=715&rft.epage=723&rft.pages=715-723&rft.issn=1044-1549&rft.eissn=1535-4989&rft.coden=AJRBEL&rft_id=info:doi/10.1165/rcmb.2007-0179OC&rft_dat=%3Cproquest_cross%3E1484185011%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=207621529&rft_id=info:pmid/18203974&rfr_iscdi=true