A FRET-based ratiometric fluorescent probe for hydrazine and its application in living cells
[Display omitted] •A ratiometric fluorescent probe (CFAc) for hydrazine was developed based on FRET mechanism for the first time. This probe was based on a coumarin–dichlorofluorescein platform.•CFAc showed clear dual-emission signal changes in blue and green channels upon addition of hydrazine unde...
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| Veröffentlicht in: | Journal of photochemistry and photobiology. A, Chemistry. Chemistry., 2018-05, Vol.358, p.10-16 |
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| container_title | Journal of photochemistry and photobiology. A, Chemistry. |
| container_volume | 358 |
| creator | Zhang, Zhanming Zhuang, Zhiyuan Song, LuLu Lin, Xi'e Zhang, Shuo Zheng, Gengxiu Zhan, Fuxu |
| description | [Display omitted]
•A ratiometric fluorescent probe (CFAc) for hydrazine was developed based on FRET mechanism for the first time. This probe was based on a coumarin–dichlorofluorescein platform.•CFAc showed clear dual-emission signal changes in blue and green channels upon addition of hydrazine under a single wavelength excitation. It exhibits high sensitivity, excellent selectivity, desirable performance to hydrazine at physiological pH in vitro.•Further, it showed low cytotoxicity and was successfully applied to dual-channel imaging of hydrazine in living cells.
A ratiometric fluorescent probe (CFAc) for hydrazine was developed based on a FRET mechanism for the first time. This probe was constructed on a new coumarin–dichlorofluorescein platform. It showed clear dual-emission signal changes in blue and green channels upon addition of hydrazine under a single wavelength excitation. Probe CFAc exhibited high sensitivity, excellent selectivity and desirable performance to hydrazine at physiological pH in vitro. Further, it showed low cytotoxicity and was successfully applied to dual-channel imaging of different hydrazine concentrations in living cells. |
| doi_str_mv | 10.1016/j.jphotochem.2018.02.005 |
| format | Article |
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•A ratiometric fluorescent probe (CFAc) for hydrazine was developed based on FRET mechanism for the first time. This probe was based on a coumarin–dichlorofluorescein platform.•CFAc showed clear dual-emission signal changes in blue and green channels upon addition of hydrazine under a single wavelength excitation. It exhibits high sensitivity, excellent selectivity, desirable performance to hydrazine at physiological pH in vitro.•Further, it showed low cytotoxicity and was successfully applied to dual-channel imaging of hydrazine in living cells.
A ratiometric fluorescent probe (CFAc) for hydrazine was developed based on a FRET mechanism for the first time. This probe was constructed on a new coumarin–dichlorofluorescein platform. It showed clear dual-emission signal changes in blue and green channels upon addition of hydrazine under a single wavelength excitation. Probe CFAc exhibited high sensitivity, excellent selectivity and desirable performance to hydrazine at physiological pH in vitro. Further, it showed low cytotoxicity and was successfully applied to dual-channel imaging of different hydrazine concentrations in living cells.</description><identifier>ISSN: 1010-6030</identifier><identifier>EISSN: 1873-2666</identifier><identifier>DOI: 10.1016/j.jphotochem.2018.02.005</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Cells (biology) ; Corrosion ; Coumarin ; Cytotoxicity ; Fluorescence ; Fluorescence resonance energy transfer ; Fluorescent indicators ; Fluorescent probe ; FRET ; Hydrazine ; Hydrazines ; Photobiology ; Photochemistry ; Ratiometric ; Toxicity ; Wave division multiplexing</subject><ispartof>Journal of photochemistry and photobiology. A, Chemistry., 2018-05, Vol.358, p.10-16</ispartof><rights>2018</rights><rights>Copyright Elsevier BV May 1, 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-a834557d9fdc765f9a62125efd86b7b30c82e15a60e6ce372b8fc0c5affde9df3</citedby><cites>FETCH-LOGICAL-c412t-a834557d9fdc765f9a62125efd86b7b30c82e15a60e6ce372b8fc0c5affde9df3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jphotochem.2018.02.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids></links><search><creatorcontrib>Zhang, Zhanming</creatorcontrib><creatorcontrib>Zhuang, Zhiyuan</creatorcontrib><creatorcontrib>Song, LuLu</creatorcontrib><creatorcontrib>Lin, Xi'e</creatorcontrib><creatorcontrib>Zhang, Shuo</creatorcontrib><creatorcontrib>Zheng, Gengxiu</creatorcontrib><creatorcontrib>Zhan, Fuxu</creatorcontrib><title>A FRET-based ratiometric fluorescent probe for hydrazine and its application in living cells</title><title>Journal of photochemistry and photobiology. A, Chemistry.</title><description>[Display omitted]
•A ratiometric fluorescent probe (CFAc) for hydrazine was developed based on FRET mechanism for the first time. This probe was based on a coumarin–dichlorofluorescein platform.•CFAc showed clear dual-emission signal changes in blue and green channels upon addition of hydrazine under a single wavelength excitation. It exhibits high sensitivity, excellent selectivity, desirable performance to hydrazine at physiological pH in vitro.•Further, it showed low cytotoxicity and was successfully applied to dual-channel imaging of hydrazine in living cells.
A ratiometric fluorescent probe (CFAc) for hydrazine was developed based on a FRET mechanism for the first time. This probe was constructed on a new coumarin–dichlorofluorescein platform. It showed clear dual-emission signal changes in blue and green channels upon addition of hydrazine under a single wavelength excitation. Probe CFAc exhibited high sensitivity, excellent selectivity and desirable performance to hydrazine at physiological pH in vitro. Further, it showed low cytotoxicity and was successfully applied to dual-channel imaging of different hydrazine concentrations in living cells.</description><subject>Cells (biology)</subject><subject>Corrosion</subject><subject>Coumarin</subject><subject>Cytotoxicity</subject><subject>Fluorescence</subject><subject>Fluorescence resonance energy transfer</subject><subject>Fluorescent indicators</subject><subject>Fluorescent probe</subject><subject>FRET</subject><subject>Hydrazine</subject><subject>Hydrazines</subject><subject>Photobiology</subject><subject>Photochemistry</subject><subject>Ratiometric</subject><subject>Toxicity</subject><subject>Wave division multiplexing</subject><issn>1010-6030</issn><issn>1873-2666</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNqFkF9LwzAUxYsoOP98h4DPrTdpm7aPc2wqDATRNyGkyY1L6ZqaZIP56e2Y4KNP9z6cc-49vyQhFDIKlN93WTduXHRqg9uMAa0zYBlAeZbMaF3lKeOcn087UEg55HCZXIXQAUBRFHSWfMzJ6nX5lrYyoCZeRuu2GL1VxPQ75zEoHCIZvWuRGOfJ5qC9_LYDEjloYmMgchx7q47GgdiB9HZvh0-isO_DTXJhZB_w9ndeJ--r5dviKV2_PD4v5utUFZTFVNZ5UZaVboxWFS9NIzmjrESja95WbQ6qZkhLyQG5wrxibW0UqFIao7HRJr9O7k65059fOwxRdG7nh-mkYFBBw1hNm0lVn1TKuxA8GjF6u5X-ICiII0vRiT-W4shSABMTy8n6cLLi1GJv0YugLA4KtfWootDO_h_yA_5Kg98</recordid><startdate>20180501</startdate><enddate>20180501</enddate><creator>Zhang, Zhanming</creator><creator>Zhuang, Zhiyuan</creator><creator>Song, LuLu</creator><creator>Lin, Xi'e</creator><creator>Zhang, Shuo</creator><creator>Zheng, Gengxiu</creator><creator>Zhan, Fuxu</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20180501</creationdate><title>A FRET-based ratiometric fluorescent probe for hydrazine and its application in living cells</title><author>Zhang, Zhanming ; Zhuang, Zhiyuan ; Song, LuLu ; Lin, Xi'e ; Zhang, Shuo ; Zheng, Gengxiu ; Zhan, Fuxu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-a834557d9fdc765f9a62125efd86b7b30c82e15a60e6ce372b8fc0c5affde9df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Cells (biology)</topic><topic>Corrosion</topic><topic>Coumarin</topic><topic>Cytotoxicity</topic><topic>Fluorescence</topic><topic>Fluorescence resonance energy transfer</topic><topic>Fluorescent indicators</topic><topic>Fluorescent probe</topic><topic>FRET</topic><topic>Hydrazine</topic><topic>Hydrazines</topic><topic>Photobiology</topic><topic>Photochemistry</topic><topic>Ratiometric</topic><topic>Toxicity</topic><topic>Wave division multiplexing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Zhanming</creatorcontrib><creatorcontrib>Zhuang, Zhiyuan</creatorcontrib><creatorcontrib>Song, LuLu</creatorcontrib><creatorcontrib>Lin, Xi'e</creatorcontrib><creatorcontrib>Zhang, Shuo</creatorcontrib><creatorcontrib>Zheng, Gengxiu</creatorcontrib><creatorcontrib>Zhan, Fuxu</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of photochemistry and photobiology. A, Chemistry.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Zhanming</au><au>Zhuang, Zhiyuan</au><au>Song, LuLu</au><au>Lin, Xi'e</au><au>Zhang, Shuo</au><au>Zheng, Gengxiu</au><au>Zhan, Fuxu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A FRET-based ratiometric fluorescent probe for hydrazine and its application in living cells</atitle><jtitle>Journal of photochemistry and photobiology. A, Chemistry.</jtitle><date>2018-05-01</date><risdate>2018</risdate><volume>358</volume><spage>10</spage><epage>16</epage><pages>10-16</pages><issn>1010-6030</issn><eissn>1873-2666</eissn><abstract>[Display omitted]
•A ratiometric fluorescent probe (CFAc) for hydrazine was developed based on FRET mechanism for the first time. This probe was based on a coumarin–dichlorofluorescein platform.•CFAc showed clear dual-emission signal changes in blue and green channels upon addition of hydrazine under a single wavelength excitation. It exhibits high sensitivity, excellent selectivity, desirable performance to hydrazine at physiological pH in vitro.•Further, it showed low cytotoxicity and was successfully applied to dual-channel imaging of hydrazine in living cells.
A ratiometric fluorescent probe (CFAc) for hydrazine was developed based on a FRET mechanism for the first time. This probe was constructed on a new coumarin–dichlorofluorescein platform. It showed clear dual-emission signal changes in blue and green channels upon addition of hydrazine under a single wavelength excitation. Probe CFAc exhibited high sensitivity, excellent selectivity and desirable performance to hydrazine at physiological pH in vitro. Further, it showed low cytotoxicity and was successfully applied to dual-channel imaging of different hydrazine concentrations in living cells.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><doi>10.1016/j.jphotochem.2018.02.005</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of photochemistry and photobiology. A, Chemistry., 2018-05, Vol.358, p.10-16 |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Cells (biology) Corrosion Coumarin Cytotoxicity Fluorescence Fluorescence resonance energy transfer Fluorescent indicators Fluorescent probe FRET Hydrazine Hydrazines Photobiology Photochemistry Ratiometric Toxicity Wave division multiplexing |
| title | A FRET-based ratiometric fluorescent probe for hydrazine and its application in living cells |
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