Preparation and Characterization of Alphitobius diaperinus Melanin
Melanin with a high antioxidant and sorption activity comparable to that of synthetic dioxyphenylalanine (DOPA)-melanin was isolated from the biomass of the darkling beetle Alphitobius diaperinus . The pigment was extracted with a solution of potassium hydroxide, followed by precipitation with conce...
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| Veröffentlicht in: | Applied biochemistry and microbiology 2018-07, Vol.54 (4), p.425-431 |
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| container_title | Applied biochemistry and microbiology |
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| creator | Ushakova, N. A. Dontsov, A. E. Sakina, N. L. Karagodin, V. P. Bastrakov, A. I. |
| description | Melanin with a high antioxidant and sorption activity comparable to that of synthetic dioxyphenylalanine (DOPA)-melanin was isolated from the biomass of the darkling beetle
Alphitobius diaperinus
. The pigment was extracted with a solution of potassium hydroxide, followed by precipitation with concentrated hydrochloric acid and hydrolysis of the resulting precipitate with the same acid. The electron paramagnetic resonance (EPR) signal of melanin was characteristic of eumelanins with a spin concentration of 4.9 × 10
17
spin per 1 g of dry weight. The melanin concentration that induced 50% inhibition of peroxidation was 9.2 μg/mL (the analogous concentration of DOPA-melanin was 8.0 μg/mL). The maximum of methylene-blue binding to the beetle melanin was 700 mg of dye per 1 g of dry weight of the preparation. The lipid-free melanin preparation exhibited antiradical activity. |
| doi_str_mv | 10.1134/S0003683818040142 |
| format | Article |
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Alphitobius diaperinus
. The pigment was extracted with a solution of potassium hydroxide, followed by precipitation with concentrated hydrochloric acid and hydrolysis of the resulting precipitate with the same acid. The electron paramagnetic resonance (EPR) signal of melanin was characteristic of eumelanins with a spin concentration of 4.9 × 10
17
spin per 1 g of dry weight. The melanin concentration that induced 50% inhibition of peroxidation was 9.2 μg/mL (the analogous concentration of DOPA-melanin was 8.0 μg/mL). The maximum of methylene-blue binding to the beetle melanin was 700 mg of dye per 1 g of dry weight of the preparation. The lipid-free melanin preparation exhibited antiradical activity.</description><identifier>ISSN: 0003-6838</identifier><identifier>EISSN: 1608-3024</identifier><identifier>DOI: 10.1134/S0003683818040142</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Alphitobius diaperinus ; Antioxidants ; Biochemistry ; Biomedical and Life Sciences ; Chemical precipitation ; Dihydroxyphenylalanine ; Electron paramagnetic resonance ; Hydrochloric acid ; Life Sciences ; Medical Microbiology ; Melanin ; Microbiology ; Peroxidation ; Potassium hydroxide ; Potassium hydroxides</subject><ispartof>Applied biochemistry and microbiology, 2018-07, Vol.54 (4), p.425-431</ispartof><rights>Pleiades Publishing, Inc. 2018</rights><rights>Applied Biochemistry and Microbiology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-ed709fb7a99439d14fd713dd701dd9723fdad11ce6c7674ee3af96c0b753c6c73</citedby><cites>FETCH-LOGICAL-c316t-ed709fb7a99439d14fd713dd701dd9723fdad11ce6c7674ee3af96c0b753c6c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0003683818040142$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0003683818040142$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids></links><search><creatorcontrib>Ushakova, N. A.</creatorcontrib><creatorcontrib>Dontsov, A. E.</creatorcontrib><creatorcontrib>Sakina, N. L.</creatorcontrib><creatorcontrib>Karagodin, V. P.</creatorcontrib><creatorcontrib>Bastrakov, A. I.</creatorcontrib><title>Preparation and Characterization of Alphitobius diaperinus Melanin</title><title>Applied biochemistry and microbiology</title><addtitle>Appl Biochem Microbiol</addtitle><description>Melanin with a high antioxidant and sorption activity comparable to that of synthetic dioxyphenylalanine (DOPA)-melanin was isolated from the biomass of the darkling beetle
Alphitobius diaperinus
. The pigment was extracted with a solution of potassium hydroxide, followed by precipitation with concentrated hydrochloric acid and hydrolysis of the resulting precipitate with the same acid. The electron paramagnetic resonance (EPR) signal of melanin was characteristic of eumelanins with a spin concentration of 4.9 × 10
17
spin per 1 g of dry weight. The melanin concentration that induced 50% inhibition of peroxidation was 9.2 μg/mL (the analogous concentration of DOPA-melanin was 8.0 μg/mL). The maximum of methylene-blue binding to the beetle melanin was 700 mg of dye per 1 g of dry weight of the preparation. The lipid-free melanin preparation exhibited antiradical activity.</description><subject>Alphitobius diaperinus</subject><subject>Antioxidants</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Chemical precipitation</subject><subject>Dihydroxyphenylalanine</subject><subject>Electron paramagnetic resonance</subject><subject>Hydrochloric acid</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Melanin</subject><subject>Microbiology</subject><subject>Peroxidation</subject><subject>Potassium hydroxide</subject><subject>Potassium hydroxides</subject><issn>0003-6838</issn><issn>1608-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1UEtLxDAQDqJgXf0B3gqeq5kmJs1xLb5gRUE9lzQPN0tNa9Ie9NebUsGDeJqZ7zXwIXQK-ByA0ItnjDFhFamgwhQDLfdQBgxXBcEl3UfZTBczf4iOYtylU7BKZOjqKZhBBjm63ufS67zepkuNJrivBextvu6GrRv71k0x104OifRpfTCd9M4fowMru2hOfuYKvd5cv9R3xebx9r5ebwpFgI2F0RwL23IpBCVCA7WaA9EJBa0FL4nVUgMowxRnnBpDpBVM4ZZfEpUwskJnS-4Q-o_JxLHZ9VPw6WVTYiYIlELMKlhUKvQxBmObIbh3GT4bwM1cVfOnquQpF09MWv9mwm_y_6ZvZ6tq5w</recordid><startdate>20180701</startdate><enddate>20180701</enddate><creator>Ushakova, N. 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A. ; Dontsov, A. E. ; Sakina, N. L. ; Karagodin, V. P. ; Bastrakov, A. I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-ed709fb7a99439d14fd713dd701dd9723fdad11ce6c7674ee3af96c0b753c6c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Alphitobius diaperinus</topic><topic>Antioxidants</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Chemical precipitation</topic><topic>Dihydroxyphenylalanine</topic><topic>Electron paramagnetic resonance</topic><topic>Hydrochloric acid</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Melanin</topic><topic>Microbiology</topic><topic>Peroxidation</topic><topic>Potassium hydroxide</topic><topic>Potassium hydroxides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ushakova, N. A.</creatorcontrib><creatorcontrib>Dontsov, A. E.</creatorcontrib><creatorcontrib>Sakina, N. L.</creatorcontrib><creatorcontrib>Karagodin, V. P.</creatorcontrib><creatorcontrib>Bastrakov, A. 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A.</au><au>Dontsov, A. E.</au><au>Sakina, N. L.</au><au>Karagodin, V. P.</au><au>Bastrakov, A. I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and Characterization of Alphitobius diaperinus Melanin</atitle><jtitle>Applied biochemistry and microbiology</jtitle><stitle>Appl Biochem Microbiol</stitle><date>2018-07-01</date><risdate>2018</risdate><volume>54</volume><issue>4</issue><spage>425</spage><epage>431</epage><pages>425-431</pages><issn>0003-6838</issn><eissn>1608-3024</eissn><abstract>Melanin with a high antioxidant and sorption activity comparable to that of synthetic dioxyphenylalanine (DOPA)-melanin was isolated from the biomass of the darkling beetle
Alphitobius diaperinus
. The pigment was extracted with a solution of potassium hydroxide, followed by precipitation with concentrated hydrochloric acid and hydrolysis of the resulting precipitate with the same acid. The electron paramagnetic resonance (EPR) signal of melanin was characteristic of eumelanins with a spin concentration of 4.9 × 10
17
spin per 1 g of dry weight. The melanin concentration that induced 50% inhibition of peroxidation was 9.2 μg/mL (the analogous concentration of DOPA-melanin was 8.0 μg/mL). The maximum of methylene-blue binding to the beetle melanin was 700 mg of dye per 1 g of dry weight of the preparation. The lipid-free melanin preparation exhibited antiradical activity.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0003683818040142</doi><tpages>7</tpages></addata></record> |
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| subjects | Alphitobius diaperinus Antioxidants Biochemistry Biomedical and Life Sciences Chemical precipitation Dihydroxyphenylalanine Electron paramagnetic resonance Hydrochloric acid Life Sciences Medical Microbiology Melanin Microbiology Peroxidation Potassium hydroxide Potassium hydroxides |
| title | Preparation and Characterization of Alphitobius diaperinus Melanin |
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