Transcriptome changes in foxtail millet genotypes at high salinity: Identification and characterization of a PHGPX gene specifically up-regulated by NaCl in a salt-tolerant line
Using a macro array filter with 711 cDNA inserts representing 620 unigenes selected from a barley EST collection, we identified transcripts differentially expressed in salt (NaCl)-treated tolerant (cv. Prasad) and sensitive (cv. Lepakshi) seedlings of foxtail millet ( Setaria italica L.). Transcript...
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Veröffentlicht in: | Journal of plant physiology 2004, Vol.161 (4), p.467-477 |
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creator | Sreenivasulu, Nese Miranda, Manoela Prakash, Harischandra Sripathy Wobus, Ulrich Weschke, Winfriede |
description | Using a macro array filter with 711 cDNA inserts representing 620 unigenes selected from a barley EST collection, we identified transcripts differentially expressed in salt (NaCl)-treated tolerant (cv. Prasad) and sensitive (cv. Lepakshi) seedlings of foxtail millet (
Setaria italica L.). Transcripts of hydrogen peroxide scavenging enzymes such as phospholipid hydroperoxide glutathione peroxidase (PHGPX), ascorbate peroxidase (APX) and catalase 1 (CAT1) in addition to some genes of cellular metabolism were found to be especially up-regulated at high salinity in the tolerant line. To analyse this process at the protein level we examined protein expression patterns under various stress conditions. A 25 kD protein with a pI of 4.8 was found to be induced prominently under high salt concentrations (250 mmol/L). This salt-induced 25 kD protein has been purified and identified by peptide sequencing as PHGPX protein. The increase of the PHGPX protein level under salt stress in the tolerant line parallels the PHGPX mRNA results of array analysis but was more pronounced. We cloned and characterized the foxtail millet PHGPX cDNA, which shows 85 percnt; and 95 percnt; homology at the DNA and protein level, respectively, to one stress-induced member of the small barley PHGPX gene family encoding non-selenium glutathione peroxidases. As shown by Southern blot analysis, a small family of PHGPX genes exists in foxtail millet, too. The specific expression pattern of the PHGPX gene in salt-induced tolerant millet seedlings suggests that its product plays an important role in the defense reaction against salt-induced oxidative damage and that the characterized glutathione peroxidase is one of the components conferring resistance against salt to the tolerant foxtail millet cultivar. |
doi_str_mv | 10.1078/0176-1617-01112 |
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Setaria italica L.). Transcripts of hydrogen peroxide scavenging enzymes such as phospholipid hydroperoxide glutathione peroxidase (PHGPX), ascorbate peroxidase (APX) and catalase 1 (CAT1) in addition to some genes of cellular metabolism were found to be especially up-regulated at high salinity in the tolerant line. To analyse this process at the protein level we examined protein expression patterns under various stress conditions. A 25 kD protein with a pI of 4.8 was found to be induced prominently under high salt concentrations (250 mmol/L). This salt-induced 25 kD protein has been purified and identified by peptide sequencing as PHGPX protein. The increase of the PHGPX protein level under salt stress in the tolerant line parallels the PHGPX mRNA results of array analysis but was more pronounced. We cloned and characterized the foxtail millet PHGPX cDNA, which shows 85 percnt; and 95 percnt; homology at the DNA and protein level, respectively, to one stress-induced member of the small barley PHGPX gene family encoding non-selenium glutathione peroxidases. As shown by Southern blot analysis, a small family of PHGPX genes exists in foxtail millet, too. The specific expression pattern of the PHGPX gene in salt-induced tolerant millet seedlings suggests that its product plays an important role in the defense reaction against salt-induced oxidative damage and that the characterized glutathione peroxidase is one of the components conferring resistance against salt to the tolerant foxtail millet cultivar.</description><identifier>ISSN: 0176-1617</identifier><identifier>EISSN: 1618-1328</identifier><identifier>DOI: 10.1078/0176-1617-01112</identifier><identifier>CODEN: JPPHEY</identifier><language>eng</language><publisher>Jena: Elsevier GmbH</publisher><subject>amino acid sequences ; Biological and medical sciences ; cDNA array analysis ; Enzymes ; Foxtail millet ( Setaria italica L.) ; Fundamental and applied biological sciences. Psychology ; gene expression regulation ; genotype ; grain crops ; hydrogen peroxide ; lipid peroxidation ; malondialdehyde ; messenger RNA ; Metabolism ; microarray technology ; millets ; molecular sequence data ; peroxidases ; phospholipid hydroperoxide glutathione peroxidase (PHGPX) ; phospholipid-hydroperoxide glutathione peroxidase ; Plant physiology and development ; plant proteins ; salinity ; salt tolerance ; salt-induced oxidative stress ; Setaria italica ; sodium chloride ; transcriptome</subject><ispartof>Journal of plant physiology, 2004, Vol.161 (4), p.467-477</ispartof><rights>2004 Urban & Fischer Verlag</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Urban & Fischer Verlag Apr 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-7df65aff02cdfaa96104603c4186560bb3a862a58d5126f5c57fb4ad16bf06843</citedby><cites>FETCH-LOGICAL-c370t-7df65aff02cdfaa96104603c4186560bb3a862a58d5126f5c57fb4ad16bf06843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/206869834?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995,64385,64389,72469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15624152$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Sreenivasulu, Nese</creatorcontrib><creatorcontrib>Miranda, Manoela</creatorcontrib><creatorcontrib>Prakash, Harischandra Sripathy</creatorcontrib><creatorcontrib>Wobus, Ulrich</creatorcontrib><creatorcontrib>Weschke, Winfriede</creatorcontrib><title>Transcriptome changes in foxtail millet genotypes at high salinity: Identification and characterization of a PHGPX gene specifically up-regulated by NaCl in a salt-tolerant line</title><title>Journal of plant physiology</title><description>Using a macro array filter with 711 cDNA inserts representing 620 unigenes selected from a barley EST collection, we identified transcripts differentially expressed in salt (NaCl)-treated tolerant (cv. Prasad) and sensitive (cv. Lepakshi) seedlings of foxtail millet (
Setaria italica L.). Transcripts of hydrogen peroxide scavenging enzymes such as phospholipid hydroperoxide glutathione peroxidase (PHGPX), ascorbate peroxidase (APX) and catalase 1 (CAT1) in addition to some genes of cellular metabolism were found to be especially up-regulated at high salinity in the tolerant line. To analyse this process at the protein level we examined protein expression patterns under various stress conditions. A 25 kD protein with a pI of 4.8 was found to be induced prominently under high salt concentrations (250 mmol/L). This salt-induced 25 kD protein has been purified and identified by peptide sequencing as PHGPX protein. The increase of the PHGPX protein level under salt stress in the tolerant line parallels the PHGPX mRNA results of array analysis but was more pronounced. We cloned and characterized the foxtail millet PHGPX cDNA, which shows 85 percnt; and 95 percnt; homology at the DNA and protein level, respectively, to one stress-induced member of the small barley PHGPX gene family encoding non-selenium glutathione peroxidases. As shown by Southern blot analysis, a small family of PHGPX genes exists in foxtail millet, too. The specific expression pattern of the PHGPX gene in salt-induced tolerant millet seedlings suggests that its product plays an important role in the defense reaction against salt-induced oxidative damage and that the characterized glutathione peroxidase is one of the components conferring resistance against salt to the tolerant foxtail millet cultivar.</description><subject>amino acid sequences</subject><subject>Biological and medical sciences</subject><subject>cDNA array analysis</subject><subject>Enzymes</subject><subject>Foxtail millet ( Setaria italica L.)</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression regulation</subject><subject>genotype</subject><subject>grain crops</subject><subject>hydrogen peroxide</subject><subject>lipid peroxidation</subject><subject>malondialdehyde</subject><subject>messenger RNA</subject><subject>Metabolism</subject><subject>microarray technology</subject><subject>millets</subject><subject>molecular sequence data</subject><subject>peroxidases</subject><subject>phospholipid hydroperoxide glutathione peroxidase (PHGPX)</subject><subject>phospholipid-hydroperoxide glutathione peroxidase</subject><subject>Plant physiology and development</subject><subject>plant proteins</subject><subject>salinity</subject><subject>salt tolerance</subject><subject>salt-induced oxidative stress</subject><subject>Setaria italica</subject><subject>sodium chloride</subject><subject>transcriptome</subject><issn>0176-1617</issn><issn>1618-1328</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kU1v1DAQhiMEEkvhzBELiWOonQ_Hyw2tSlupgkq0Ejdr4oyzrrxxsL2I8K_6D2s3FZw4WeN55n3noyjeMvqR0U6cUtbxknHWlZQxVj0rNikQJasr8bzY_M2-LF6FcEdT3Ip6U9zfeJiC8maO7oBE7WEaMRAzEe1-RzCWHIy1GMmIk4vLnHIQyd6MexLAmsnE5RO5HHCKRhsF0biJwDRkIQ8qojd_1k-nCZDri_PrH1kKSZhRPZZYu5DjXHocjxYiDqRfyFfY2dwDZJNYRmcxtRlJMsTXxQsNNuCbp_ekuP1ydrO7KK--nV_uPl-Vqu5oLLtB8xa0ppUaNMCWM9pwWquGCd5y2vc1CF5BK4aWVVy3qu1038DAeK8pF019UrxfdWfvfh4xRHnnjn5KlrJKAN-KOkOnK6S8C8GjlrM3B_CLZFTms8i8eJkXLx_Pkio-PMlCSMPrNJcy4V9Zy6uGtZl7t3IanITRJ-b2e0VZTem2FlWXie1KYNrCL4NeBmVwUjgYjyrKwZn_dvEAMQ-q7g</recordid><startdate>2004</startdate><enddate>2004</enddate><creator>Sreenivasulu, Nese</creator><creator>Miranda, Manoela</creator><creator>Prakash, Harischandra Sripathy</creator><creator>Wobus, Ulrich</creator><creator>Weschke, Winfriede</creator><general>Elsevier GmbH</general><general>Elsevier</general><general>Elsevier Science Ltd</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7SS</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>S0X</scope></search><sort><creationdate>2004</creationdate><title>Transcriptome changes in foxtail millet genotypes at high salinity: Identification and characterization of a PHGPX gene specifically up-regulated by NaCl in a salt-tolerant line</title><author>Sreenivasulu, Nese ; Miranda, Manoela ; Prakash, Harischandra Sripathy ; Wobus, Ulrich ; Weschke, Winfriede</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-7df65aff02cdfaa96104603c4186560bb3a862a58d5126f5c57fb4ad16bf06843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>amino acid sequences</topic><topic>Biological and medical sciences</topic><topic>cDNA array analysis</topic><topic>Enzymes</topic><topic>Foxtail millet ( Setaria italica L.)</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression regulation</topic><topic>genotype</topic><topic>grain crops</topic><topic>hydrogen peroxide</topic><topic>lipid peroxidation</topic><topic>malondialdehyde</topic><topic>messenger RNA</topic><topic>Metabolism</topic><topic>microarray technology</topic><topic>millets</topic><topic>molecular sequence data</topic><topic>peroxidases</topic><topic>phospholipid hydroperoxide glutathione peroxidase (PHGPX)</topic><topic>phospholipid-hydroperoxide glutathione peroxidase</topic><topic>Plant physiology and development</topic><topic>plant proteins</topic><topic>salinity</topic><topic>salt tolerance</topic><topic>salt-induced oxidative stress</topic><topic>Setaria italica</topic><topic>sodium chloride</topic><topic>transcriptome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sreenivasulu, Nese</creatorcontrib><creatorcontrib>Miranda, Manoela</creatorcontrib><creatorcontrib>Prakash, Harischandra Sripathy</creatorcontrib><creatorcontrib>Wobus, Ulrich</creatorcontrib><creatorcontrib>Weschke, Winfriede</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>SIRS Editorial</collection><jtitle>Journal of plant physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sreenivasulu, Nese</au><au>Miranda, Manoela</au><au>Prakash, Harischandra Sripathy</au><au>Wobus, Ulrich</au><au>Weschke, Winfriede</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptome changes in foxtail millet genotypes at high salinity: Identification and characterization of a PHGPX gene specifically up-regulated by NaCl in a salt-tolerant line</atitle><jtitle>Journal of plant physiology</jtitle><date>2004</date><risdate>2004</risdate><volume>161</volume><issue>4</issue><spage>467</spage><epage>477</epage><pages>467-477</pages><issn>0176-1617</issn><eissn>1618-1328</eissn><coden>JPPHEY</coden><abstract>Using a macro array filter with 711 cDNA inserts representing 620 unigenes selected from a barley EST collection, we identified transcripts differentially expressed in salt (NaCl)-treated tolerant (cv. Prasad) and sensitive (cv. Lepakshi) seedlings of foxtail millet (
Setaria italica L.). Transcripts of hydrogen peroxide scavenging enzymes such as phospholipid hydroperoxide glutathione peroxidase (PHGPX), ascorbate peroxidase (APX) and catalase 1 (CAT1) in addition to some genes of cellular metabolism were found to be especially up-regulated at high salinity in the tolerant line. To analyse this process at the protein level we examined protein expression patterns under various stress conditions. A 25 kD protein with a pI of 4.8 was found to be induced prominently under high salt concentrations (250 mmol/L). This salt-induced 25 kD protein has been purified and identified by peptide sequencing as PHGPX protein. The increase of the PHGPX protein level under salt stress in the tolerant line parallels the PHGPX mRNA results of array analysis but was more pronounced. We cloned and characterized the foxtail millet PHGPX cDNA, which shows 85 percnt; and 95 percnt; homology at the DNA and protein level, respectively, to one stress-induced member of the small barley PHGPX gene family encoding non-selenium glutathione peroxidases. As shown by Southern blot analysis, a small family of PHGPX genes exists in foxtail millet, too. The specific expression pattern of the PHGPX gene in salt-induced tolerant millet seedlings suggests that its product plays an important role in the defense reaction against salt-induced oxidative damage and that the characterized glutathione peroxidase is one of the components conferring resistance against salt to the tolerant foxtail millet cultivar.</abstract><cop>Jena</cop><pub>Elsevier GmbH</pub><doi>10.1078/0176-1617-01112</doi><tpages>11</tpages></addata></record> |
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subjects | amino acid sequences Biological and medical sciences cDNA array analysis Enzymes Foxtail millet ( Setaria italica L.) Fundamental and applied biological sciences. Psychology gene expression regulation genotype grain crops hydrogen peroxide lipid peroxidation malondialdehyde messenger RNA Metabolism microarray technology millets molecular sequence data peroxidases phospholipid hydroperoxide glutathione peroxidase (PHGPX) phospholipid-hydroperoxide glutathione peroxidase Plant physiology and development plant proteins salinity salt tolerance salt-induced oxidative stress Setaria italica sodium chloride transcriptome |
title | Transcriptome changes in foxtail millet genotypes at high salinity: Identification and characterization of a PHGPX gene specifically up-regulated by NaCl in a salt-tolerant line |
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