Ultrasensitive, label-free detection of T4 ligase and T4 polynucleotide kinase based on target-triggered hyper-branched rolling circle amplification

•A simple but sensitive sensor is established for ligase and polynucleotide kinase (PNK) assay.•Exponential HRCA confers the sensor with high sensitivity and selectivity.•Label-free detection mode greatly simplifies the sensor design and reduces the cost.•The sensor was successfully used for ultrase...

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Veröffentlicht in:	Sensors and actuators. B, Chemical Chemical, 2018-05, Vol.260, p.70-77
Hauptverfasser:	Jiang, Hongxin, Xu, Yaping, Dai, Lihong, Liu, Xiaowei, Kong, Deming
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Biosensors Deoxyribonucleic acid DNA Enzymes Fluorescence Fluorescence biosensor Hyper-branched rolling circle amplification Kinases Label-free Ligase Molecular chains Polynucleotide kinase Sensitivity analysis Studies
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