Carbon paste electrode based on functional GOx/silica-lignin system to prepare an amperometric glucose biosensor

[Display omitted] •Biosensor with promising electrochemical parameters for glucose determination.•Immobilization of GOx and the development of stable glucose biosensors.•Carbon paste electrode based on functional GOx/silica/lignin system is prepare. In this paper, a novel procedure for receiving enzyme biosensor based on the cheap and functional silica/lignin (SiO2/Lig) hybrid material is presented. In this study, a functional biohybrid SiO2/Lig was selected to conduct the immobilization of glucose oxidase (GOx) by adsorption on its surface. The immobilized amount of GOx at SiO2/Lig was 25.28mgg−1, twice as much compared to its amount at non-functionalized SiO2. The system of GOx-SiO2/Lig was combined with single-walled carbon nanotubes/platinum nanoparticles support to prepare a I generation glucose biosensor. Moreover, the GOx-SiO2/Lig based carbon paste electrode with ferrocene redox mediator was evaluated as an active material in II generation glucose biosensor. The GOx-SiO2/Lig/CPE was subjected to an examination in glucose solution by electrochemical techniques such as cyclic voltammetry (CV) and chronoamperometry. The obtained results suggest that GOx-SiO2/Lig can be a material of choice for preparation of an efficient and low-cost biosensor working in various electrode configurations. The newly obtained glucose biosensor presents promising electrochemical parameters for glucose determination. The glucose-sensing sensitivity amounted 0.78μAmM−1. The biosensor showed a linear response range of 0.5–9mM with a detection limit (LOD) of 145μM.