High biobutanol production integrated with in situ extraction in the presence of Tween 80 by Clostridium acetobutylicum
[Display omitted] •The butanol titer was enhanced to 18.65 g/L in the presence of 15% Tween 80.•Tween 80 could significantly improve butanol tolerance up to 18 g/L.•Tween 80 could improve butanol dehydrogenase activities.•In situ extraction using biodiesel gave a high butanol production of 33.90 g/L...
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| Veröffentlicht in: | Process biochemistry (1991) 2018-04, Vol.67, p.113-117 |
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| container_title | Process biochemistry (1991) |
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| creator | Xin, Fengxue Liu, Jie He, Mingxiong Wu, Bo Ni, Yufan Dong, Weiliang Zhang, Wenming Hu, Guoquan Jiang, Min |
| description | [Display omitted]
•The butanol titer was enhanced to 18.65 g/L in the presence of 15% Tween 80.•Tween 80 could significantly improve butanol tolerance up to 18 g/L.•Tween 80 could improve butanol dehydrogenase activities.•In situ extraction using biodiesel gave a high butanol production of 33.90 g/L.
This study investigates the improvement of butanol production in the presence – Tween 80 by Clostridium acetobutylicum NJ4. With supplementation of 15% Tween 80 (v/v), the final butanol titer could be significantly enhanced to 18.65 g/L from 70 g/L glucose, which is 38% higher than that in the control batch (13.47 g/L). With further in situ extraction using biodiesel, the butanol titer was finally improved to 33.90 g/L in the fed batch mode. The supplementation of Tween 80 could improve butanol tolerance of strain NJ4 up to 18 g/L compared to 10 g/L of control. Meanwhile, butanol dehydrogenase activity was improved to 3.89 U/mg, which is 80.93% higher than that of the control. These factors may attribute to the high butanol production. The finding of this study thus offers fundamental knowledge for the future development of alternative ABE production strategies. |
| doi_str_mv | 10.1016/j.procbio.2018.01.013 |
| format | Article |
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•The butanol titer was enhanced to 18.65 g/L in the presence of 15% Tween 80.•Tween 80 could significantly improve butanol tolerance up to 18 g/L.•Tween 80 could improve butanol dehydrogenase activities.•In situ extraction using biodiesel gave a high butanol production of 33.90 g/L.
This study investigates the improvement of butanol production in the presence – Tween 80 by Clostridium acetobutylicum NJ4. With supplementation of 15% Tween 80 (v/v), the final butanol titer could be significantly enhanced to 18.65 g/L from 70 g/L glucose, which is 38% higher than that in the control batch (13.47 g/L). With further in situ extraction using biodiesel, the butanol titer was finally improved to 33.90 g/L in the fed batch mode. The supplementation of Tween 80 could improve butanol tolerance of strain NJ4 up to 18 g/L compared to 10 g/L of control. Meanwhile, butanol dehydrogenase activity was improved to 3.89 U/mg, which is 80.93% higher than that of the control. These factors may attribute to the high butanol production. The finding of this study thus offers fundamental knowledge for the future development of alternative ABE production strategies.</description><identifier>ISSN: 1359-5113</identifier><identifier>EISSN: 1873-3298</identifier><identifier>DOI: 10.1016/j.procbio.2018.01.013</identifier><language>eng</language><publisher>Barking: Elsevier Ltd</publisher><subject>Bacteria ; Biobutanol ; Biodiesel fuels ; Biofuels ; Butanol ; Chemical bonds ; Clostridium ; Dehydrogenases ; Extraction processes ; Fed batch ; Fermentation ; In situ removal ; Non-ionic surfactant ; Surfactants ; Tolerance fed batch fermentation</subject><ispartof>Process biochemistry (1991), 2018-04, Vol.67, p.113-117</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright Elsevier BV Apr 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c374t-1d722d7606ca218328021785aae907752d8c49a7fff3f042ee731d78610f61f23</citedby><cites>FETCH-LOGICAL-c374t-1d722d7606ca218328021785aae907752d8c49a7fff3f042ee731d78610f61f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S135951131731680X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids></links><search><creatorcontrib>Xin, Fengxue</creatorcontrib><creatorcontrib>Liu, Jie</creatorcontrib><creatorcontrib>He, Mingxiong</creatorcontrib><creatorcontrib>Wu, Bo</creatorcontrib><creatorcontrib>Ni, Yufan</creatorcontrib><creatorcontrib>Dong, Weiliang</creatorcontrib><creatorcontrib>Zhang, Wenming</creatorcontrib><creatorcontrib>Hu, Guoquan</creatorcontrib><creatorcontrib>Jiang, Min</creatorcontrib><title>High biobutanol production integrated with in situ extraction in the presence of Tween 80 by Clostridium acetobutylicum</title><title>Process biochemistry (1991)</title><description>[Display omitted]
•The butanol titer was enhanced to 18.65 g/L in the presence of 15% Tween 80.•Tween 80 could significantly improve butanol tolerance up to 18 g/L.•Tween 80 could improve butanol dehydrogenase activities.•In situ extraction using biodiesel gave a high butanol production of 33.90 g/L.
This study investigates the improvement of butanol production in the presence – Tween 80 by Clostridium acetobutylicum NJ4. With supplementation of 15% Tween 80 (v/v), the final butanol titer could be significantly enhanced to 18.65 g/L from 70 g/L glucose, which is 38% higher than that in the control batch (13.47 g/L). With further in situ extraction using biodiesel, the butanol titer was finally improved to 33.90 g/L in the fed batch mode. The supplementation of Tween 80 could improve butanol tolerance of strain NJ4 up to 18 g/L compared to 10 g/L of control. Meanwhile, butanol dehydrogenase activity was improved to 3.89 U/mg, which is 80.93% higher than that of the control. These factors may attribute to the high butanol production. The finding of this study thus offers fundamental knowledge for the future development of alternative ABE production strategies.</description><subject>Bacteria</subject><subject>Biobutanol</subject><subject>Biodiesel fuels</subject><subject>Biofuels</subject><subject>Butanol</subject><subject>Chemical bonds</subject><subject>Clostridium</subject><subject>Dehydrogenases</subject><subject>Extraction processes</subject><subject>Fed batch</subject><subject>Fermentation</subject><subject>In situ removal</subject><subject>Non-ionic surfactant</subject><subject>Surfactants</subject><subject>Tolerance fed batch fermentation</subject><issn>1359-5113</issn><issn>1873-3298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNqFkEFLAzEQhRdRsFZ_ghDwvDWT7G7Sk0hRKxS81HNIs5M2pd3UJGvtvzel9SwMJAPve495RXEPdAQUmsf1aBe8WTg_YhTkiEIeflEMQApecjaWl_nP63FZA_Dr4ibGNaUcAOig2E_dckUyu-iT7vyGZKu2N8n5jrgu4TLohC3Zu7TKO4ku9QR_UtB_EpJWmCGM2Bkk3pL5HrEjkpLFgUw2PqbgWtdviTaYjimHjTP99ra4snoT8e78DovP15f5ZFrOPt7eJ8-z0nBRpRJawVgrGtoYzUByJikDIWutcUyFqFkrTTXWwlrLLa0YouCZkQ1Q24BlfFg8nHzzXV89xqTWvg9djlSMNjmjkVWVVfVJZYKPMaBVu-C2OhwUUHXsWK3VuWN17FhRyMMz93TiMJ_w7TCoaNyxiNYFNEm13v3j8AtdeIjc</recordid><startdate>20180401</startdate><enddate>20180401</enddate><creator>Xin, Fengxue</creator><creator>Liu, Jie</creator><creator>He, Mingxiong</creator><creator>Wu, Bo</creator><creator>Ni, Yufan</creator><creator>Dong, Weiliang</creator><creator>Zhang, Wenming</creator><creator>Hu, Guoquan</creator><creator>Jiang, Min</creator><general>Elsevier Ltd</general><general>Elsevier BV</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20180401</creationdate><title>High biobutanol production integrated with in situ extraction in the presence of Tween 80 by Clostridium acetobutylicum</title><author>Xin, Fengxue ; Liu, Jie ; He, Mingxiong ; Wu, Bo ; Ni, Yufan ; Dong, Weiliang ; Zhang, Wenming ; Hu, Guoquan ; Jiang, Min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-1d722d7606ca218328021785aae907752d8c49a7fff3f042ee731d78610f61f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Bacteria</topic><topic>Biobutanol</topic><topic>Biodiesel fuels</topic><topic>Biofuels</topic><topic>Butanol</topic><topic>Chemical bonds</topic><topic>Clostridium</topic><topic>Dehydrogenases</topic><topic>Extraction processes</topic><topic>Fed batch</topic><topic>Fermentation</topic><topic>In situ removal</topic><topic>Non-ionic surfactant</topic><topic>Surfactants</topic><topic>Tolerance fed batch fermentation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xin, Fengxue</creatorcontrib><creatorcontrib>Liu, Jie</creatorcontrib><creatorcontrib>He, Mingxiong</creatorcontrib><creatorcontrib>Wu, Bo</creatorcontrib><creatorcontrib>Ni, Yufan</creatorcontrib><creatorcontrib>Dong, Weiliang</creatorcontrib><creatorcontrib>Zhang, Wenming</creatorcontrib><creatorcontrib>Hu, Guoquan</creatorcontrib><creatorcontrib>Jiang, Min</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Process biochemistry (1991)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xin, Fengxue</au><au>Liu, Jie</au><au>He, Mingxiong</au><au>Wu, Bo</au><au>Ni, Yufan</au><au>Dong, Weiliang</au><au>Zhang, Wenming</au><au>Hu, Guoquan</au><au>Jiang, Min</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High biobutanol production integrated with in situ extraction in the presence of Tween 80 by Clostridium acetobutylicum</atitle><jtitle>Process biochemistry (1991)</jtitle><date>2018-04-01</date><risdate>2018</risdate><volume>67</volume><spage>113</spage><epage>117</epage><pages>113-117</pages><issn>1359-5113</issn><eissn>1873-3298</eissn><abstract>[Display omitted]
•The butanol titer was enhanced to 18.65 g/L in the presence of 15% Tween 80.•Tween 80 could significantly improve butanol tolerance up to 18 g/L.•Tween 80 could improve butanol dehydrogenase activities.•In situ extraction using biodiesel gave a high butanol production of 33.90 g/L.
This study investigates the improvement of butanol production in the presence – Tween 80 by Clostridium acetobutylicum NJ4. With supplementation of 15% Tween 80 (v/v), the final butanol titer could be significantly enhanced to 18.65 g/L from 70 g/L glucose, which is 38% higher than that in the control batch (13.47 g/L). With further in situ extraction using biodiesel, the butanol titer was finally improved to 33.90 g/L in the fed batch mode. The supplementation of Tween 80 could improve butanol tolerance of strain NJ4 up to 18 g/L compared to 10 g/L of control. Meanwhile, butanol dehydrogenase activity was improved to 3.89 U/mg, which is 80.93% higher than that of the control. These factors may attribute to the high butanol production. The finding of this study thus offers fundamental knowledge for the future development of alternative ABE production strategies.</abstract><cop>Barking</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.procbio.2018.01.013</doi><tpages>5</tpages></addata></record> |
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| subjects | Bacteria Biobutanol Biodiesel fuels Biofuels Butanol Chemical bonds Clostridium Dehydrogenases Extraction processes Fed batch Fermentation In situ removal Non-ionic surfactant Surfactants Tolerance fed batch fermentation |
| title | High biobutanol production integrated with in situ extraction in the presence of Tween 80 by Clostridium acetobutylicum |
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