Involvement of Pyruvate Oxidase Activity and Acetate Production in the Survival of Lactobacillus plantarum during the Stationary Phase of Aerobic Growth
In addition to the previously characterized pyruvate oxidase PoxB, the Lactobacillus plantarum genome encodes four predicted pyruvate oxidases (PoxC, PoxD, PoxE, and PoxF). Each pyruvate oxidase gene was individually inactivated, and only the knockout of poxF resulted in a decrease in pyruvate oxida...
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| Veröffentlicht in: | Applied and Environmental Microbiology 2006-12, Vol.72 (12), p.7933-7940 |
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| creator | Goffin, Philippe Muscariello, Lidia Lorquet, Frederique Stukkens, Aline Prozzi, Deborah Sacco, Margherita Kleerebezem, Michiel Hols, Pascal |
| description | In addition to the previously characterized pyruvate oxidase PoxB, the Lactobacillus plantarum genome encodes four predicted pyruvate oxidases (PoxC, PoxD, PoxE, and PoxF). Each pyruvate oxidase gene was individually inactivated, and only the knockout of poxF resulted in a decrease in pyruvate oxidase activity under the tested conditions. We show here that L. plantarum has two major pyruvate oxidases: PoxB and PoxF. Both are involved in lactate-to-acetate conversion in the early stationary phase of aerobic growth and are regulated by carbon catabolite repression. A strain devoid of pyruvate oxidase activity was constructed by knocking out the poxB and poxF genes. In this mutant, acetate production was strongly affected, with lactate remaining the major end product of either glucose or maltose fermentation. Notably, survival during the stationary phase appeared to be dramatically improved in the poxB poxF double mutant. |
| doi_str_mv | 10.1128/AEM.00659-06 |
| format | Article |
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Each pyruvate oxidase gene was individually inactivated, and only the knockout of poxF resulted in a decrease in pyruvate oxidase activity under the tested conditions. We show here that L. plantarum has two major pyruvate oxidases: PoxB and PoxF. Both are involved in lactate-to-acetate conversion in the early stationary phase of aerobic growth and are regulated by carbon catabolite repression. A strain devoid of pyruvate oxidase activity was constructed by knocking out the poxB and poxF genes. In this mutant, acetate production was strongly affected, with lactate remaining the major end product of either glucose or maltose fermentation. Notably, survival during the stationary phase appeared to be dramatically improved in the poxB poxF double mutant.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.00659-06</identifier><identifier>PMID: 17012588</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Acetates - metabolism ; Aerobiosis ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Base Sequence ; Biological and medical sciences ; Biotechnology - methods ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; Genes ; Genomics ; Glucose ; Lactobacillus plantarum ; Lactobacillus plantarum - enzymology ; Lactobacillus plantarum - genetics ; Lactobacillus plantarum - growth & development ; Lactobacillus plantarum - metabolism ; Microbiology ; Molecular Sequence Data ; Mutation ; Physiology and Biotechnology ; Pyruvate Oxidase - genetics ; Pyruvate Oxidase - metabolism</subject><ispartof>Applied and Environmental Microbiology, 2006-12, Vol.72 (12), p.7933-7940</ispartof><rights>2007 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Dec 2006</rights><rights>Copyright © 2006, American Society for Microbiology 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-736dcae77e50215f3415c6fc4ef6e42949e13003a689f74c94125419898618883</citedby><cites>FETCH-LOGICAL-c521t-736dcae77e50215f3415c6fc4ef6e42949e13003a689f74c94125419898618883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1694206/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1694206/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18352694$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17012588$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goffin, Philippe</creatorcontrib><creatorcontrib>Muscariello, Lidia</creatorcontrib><creatorcontrib>Lorquet, Frederique</creatorcontrib><creatorcontrib>Stukkens, Aline</creatorcontrib><creatorcontrib>Prozzi, Deborah</creatorcontrib><creatorcontrib>Sacco, Margherita</creatorcontrib><creatorcontrib>Kleerebezem, Michiel</creatorcontrib><creatorcontrib>Hols, Pascal</creatorcontrib><title>Involvement of Pyruvate Oxidase Activity and Acetate Production in the Survival of Lactobacillus plantarum during the Stationary Phase of Aerobic Growth</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>In addition to the previously characterized pyruvate oxidase PoxB, the Lactobacillus plantarum genome encodes four predicted pyruvate oxidases (PoxC, PoxD, PoxE, and PoxF). Each pyruvate oxidase gene was individually inactivated, and only the knockout of poxF resulted in a decrease in pyruvate oxidase activity under the tested conditions. We show here that L. plantarum has two major pyruvate oxidases: PoxB and PoxF. Both are involved in lactate-to-acetate conversion in the early stationary phase of aerobic growth and are regulated by carbon catabolite repression. A strain devoid of pyruvate oxidase activity was constructed by knocking out the poxB and poxF genes. In this mutant, acetate production was strongly affected, with lactate remaining the major end product of either glucose or maltose fermentation. 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Psychology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes</topic><topic>Genomics</topic><topic>Glucose</topic><topic>Lactobacillus plantarum</topic><topic>Lactobacillus plantarum - enzymology</topic><topic>Lactobacillus plantarum - genetics</topic><topic>Lactobacillus plantarum - growth & development</topic><topic>Lactobacillus plantarum - metabolism</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Physiology and Biotechnology</topic><topic>Pyruvate Oxidase - genetics</topic><topic>Pyruvate Oxidase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goffin, Philippe</creatorcontrib><creatorcontrib>Muscariello, Lidia</creatorcontrib><creatorcontrib>Lorquet, Frederique</creatorcontrib><creatorcontrib>Stukkens, Aline</creatorcontrib><creatorcontrib>Prozzi, Deborah</creatorcontrib><creatorcontrib>Sacco, Margherita</creatorcontrib><creatorcontrib>Kleerebezem, Michiel</creatorcontrib><creatorcontrib>Hols, Pascal</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goffin, Philippe</au><au>Muscariello, Lidia</au><au>Lorquet, Frederique</au><au>Stukkens, Aline</au><au>Prozzi, Deborah</au><au>Sacco, Margherita</au><au>Kleerebezem, Michiel</au><au>Hols, Pascal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involvement of Pyruvate Oxidase Activity and Acetate Production in the Survival of Lactobacillus plantarum during the Stationary Phase of Aerobic Growth</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2006-12-01</date><risdate>2006</risdate><volume>72</volume><issue>12</issue><spage>7933</spage><epage>7940</epage><pages>7933-7940</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>In addition to the previously characterized pyruvate oxidase PoxB, the Lactobacillus plantarum genome encodes four predicted pyruvate oxidases (PoxC, PoxD, PoxE, and PoxF). 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| subjects | Acetates - metabolism Aerobiosis Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Biological and medical sciences Biotechnology - methods Fermentation Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Genes Genomics Glucose Lactobacillus plantarum Lactobacillus plantarum - enzymology Lactobacillus plantarum - genetics Lactobacillus plantarum - growth & development Lactobacillus plantarum - metabolism Microbiology Molecular Sequence Data Mutation Physiology and Biotechnology Pyruvate Oxidase - genetics Pyruvate Oxidase - metabolism |
| title | Involvement of Pyruvate Oxidase Activity and Acetate Production in the Survival of Lactobacillus plantarum during the Stationary Phase of Aerobic Growth |
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