Effect of Nitrate and Acetylene on nirS, cnorB, and nosZ Expression and Denitrification Activity in Pseudomonas mandelii
Nitrate acts as an electron acceptor in the denitrification process. The effect of nitrate in the range of 0 to 1,000 mg/liter on Pseudomonas mandelii nirS, cnorB, and nosZ gene expression was studied, using quantitative reverse transcription-quantitative PCR. Denitrification activity was measured b...
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| Veröffentlicht in: | Applied and Environmental Microbiology 2009-08, Vol.75 (15), p.5082-5087 |
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| creator | Saleh-Lakha, Saleema Shannon, Kelly E Henderson, Sherri L Zebarth, Bernie J Burton, David L Goyer, Claudia Trevors, Jack T |
| description | Nitrate acts as an electron acceptor in the denitrification process. The effect of nitrate in the range of 0 to 1,000 mg/liter on Pseudomonas mandelii nirS, cnorB, and nosZ gene expression was studied, using quantitative reverse transcription-quantitative PCR. Denitrification activity was measured by using the acetylene blockage method and gas chromatography. The effect of acetylene on gene expression was assessed by comparing denitrification gene expression in P. mandelii culture grown in the presence or absence of acetylene. The higher the amount of NO₃⁻ present, the greater the induction and the longer the denitrification genes remained expressed. nirS gene expression reached a maximum at 2, 4, 4, and 6 h in cultures grown in the presence of 0, 10, 100, and 1,000 mg of KNO₃/liter, respectively, while induction of nirS gene ranged from 12- to 225-fold compared to time zero. cnorB gene expression also followed a similar trend. nosZ gene expression did not respond to NO₃⁻ treatment under the conditions tested. Acetylene decreased nosZ gene expression but did not affect nirS or cnorB gene expression. These results showed that nirS and cnorB responded to nitrate concentrations; however, significant denitrification activity was only observed in culture with 1,000 mg of KNO₃/liter, indicating that there was no relationship between gene expression and denitrification activity under the conditions tested. |
| doi_str_mv | 10.1128/AEM.00777-09 |
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The effect of nitrate in the range of 0 to 1,000 mg/liter on Pseudomonas mandelii nirS, cnorB, and nosZ gene expression was studied, using quantitative reverse transcription-quantitative PCR. Denitrification activity was measured by using the acetylene blockage method and gas chromatography. The effect of acetylene on gene expression was assessed by comparing denitrification gene expression in P. mandelii culture grown in the presence or absence of acetylene. The higher the amount of NO₃⁻ present, the greater the induction and the longer the denitrification genes remained expressed. nirS gene expression reached a maximum at 2, 4, 4, and 6 h in cultures grown in the presence of 0, 10, 100, and 1,000 mg of KNO₃/liter, respectively, while induction of nirS gene ranged from 12- to 225-fold compared to time zero. cnorB gene expression also followed a similar trend. nosZ gene expression did not respond to NO₃⁻ treatment under the conditions tested. Acetylene decreased nosZ gene expression but did not affect nirS or cnorB gene expression. These results showed that nirS and cnorB responded to nitrate concentrations; however, significant denitrification activity was only observed in culture with 1,000 mg of KNO₃/liter, indicating that there was no relationship between gene expression and denitrification activity under the conditions tested.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/AEM.00777-09</identifier><identifier>PMID: 19525277</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Acetylene - metabolism ; Bacteria ; Bacterial Proteins - biosynthesis ; Chromatography ; Denitrification ; Effects ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial ; Gene Expression Regulation, Enzymologic ; Microbiology ; Nitrates ; Nitrates - metabolism ; Physiology ; Pseudomonas ; Pseudomonas - growth & development ; Pseudomonas - metabolism</subject><ispartof>Applied and Environmental Microbiology, 2009-08, Vol.75 (15), p.5082-5087</ispartof><rights>Copyright American Society for Microbiology Aug 2009</rights><rights>Copyright © 2009, American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-9f11418f3d2223c38894645cc4f92b9ffdd44a6c3aa6f4abbe0b988996aad2773</citedby><cites>FETCH-LOGICAL-c491t-9f11418f3d2223c38894645cc4f92b9ffdd44a6c3aa6f4abbe0b988996aad2773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2725510/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2725510/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,3189,3190,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19525277$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saleh-Lakha, Saleema</creatorcontrib><creatorcontrib>Shannon, Kelly E</creatorcontrib><creatorcontrib>Henderson, Sherri L</creatorcontrib><creatorcontrib>Zebarth, Bernie J</creatorcontrib><creatorcontrib>Burton, David L</creatorcontrib><creatorcontrib>Goyer, Claudia</creatorcontrib><creatorcontrib>Trevors, Jack T</creatorcontrib><title>Effect of Nitrate and Acetylene on nirS, cnorB, and nosZ Expression and Denitrification Activity in Pseudomonas mandelii</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Nitrate acts as an electron acceptor in the denitrification process. The effect of nitrate in the range of 0 to 1,000 mg/liter on Pseudomonas mandelii nirS, cnorB, and nosZ gene expression was studied, using quantitative reverse transcription-quantitative PCR. Denitrification activity was measured by using the acetylene blockage method and gas chromatography. The effect of acetylene on gene expression was assessed by comparing denitrification gene expression in P. mandelii culture grown in the presence or absence of acetylene. The higher the amount of NO₃⁻ present, the greater the induction and the longer the denitrification genes remained expressed. nirS gene expression reached a maximum at 2, 4, 4, and 6 h in cultures grown in the presence of 0, 10, 100, and 1,000 mg of KNO₃/liter, respectively, while induction of nirS gene ranged from 12- to 225-fold compared to time zero. cnorB gene expression also followed a similar trend. nosZ gene expression did not respond to NO₃⁻ treatment under the conditions tested. Acetylene decreased nosZ gene expression but did not affect nirS or cnorB gene expression. These results showed that nirS and cnorB responded to nitrate concentrations; however, significant denitrification activity was only observed in culture with 1,000 mg of KNO₃/liter, indicating that there was no relationship between gene expression and denitrification activity under the conditions tested.</description><subject>Acetylene - metabolism</subject><subject>Bacteria</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Chromatography</subject><subject>Denitrification</subject><subject>Effects</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Microbiology</subject><subject>Nitrates</subject><subject>Nitrates - metabolism</subject><subject>Physiology</subject><subject>Pseudomonas</subject><subject>Pseudomonas - growth & development</subject><subject>Pseudomonas - metabolism</subject><issn>0099-2240</issn><issn>1098-5336</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhSMEokNhxxosFqwm5fqVxBukoR0eUnlIpRs2lsexZ1wl9mAnbeff43RGFNggLyzd-_nce3yK4jmGE4xJ82ax_HwCUNd1CeJBMcMgmpJTWj0sZgBClIQwOCqepHQFAAyq5nFxhAUnnNT1rLhdWmv0gIJFX9wQ1WCQ8i1aaDPsOuMNCh55Fy_mSPsQ383vuj6kH2h5u40mJZeBqXZmfH7vrNNqmGoLPbhrN-yQ8-hbMmMb-uBVQn2GTefc0-KRVV0yzw73cXH5fvn99GN5_vXDp9PFeamZwEMpLMYMN5a2hBCqadMIVjGuNbOCrIS1bcuYqjRVqrJMrVYGViJDolKqzQ7pcfF2r7sdV71ptfHZZSe30fUq7mRQTv7d8W4j1-FakppwjiELvD4IxPBzNGmQvUvadJ3yJoxJVjUnVAD5L0gwa3gjppVe_QNehTH6_AuSABf5EJGh-R7SMaQUjf29MgY5BS9z8PIueAkT_uJPm_fwIen7oRu33ty4aKRKvVSmlzWXmEsOzWTh5R6yKki1ji7JywsCmAKueMMqQX8BAZi-bQ</recordid><startdate>20090801</startdate><enddate>20090801</enddate><creator>Saleh-Lakha, Saleema</creator><creator>Shannon, Kelly E</creator><creator>Henderson, Sherri L</creator><creator>Zebarth, Bernie J</creator><creator>Burton, David L</creator><creator>Goyer, Claudia</creator><creator>Trevors, Jack T</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090801</creationdate><title>Effect of Nitrate and Acetylene on nirS, cnorB, and nosZ Expression and Denitrification Activity in Pseudomonas mandelii</title><author>Saleh-Lakha, Saleema ; 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The effect of nitrate in the range of 0 to 1,000 mg/liter on Pseudomonas mandelii nirS, cnorB, and nosZ gene expression was studied, using quantitative reverse transcription-quantitative PCR. Denitrification activity was measured by using the acetylene blockage method and gas chromatography. The effect of acetylene on gene expression was assessed by comparing denitrification gene expression in P. mandelii culture grown in the presence or absence of acetylene. The higher the amount of NO₃⁻ present, the greater the induction and the longer the denitrification genes remained expressed. nirS gene expression reached a maximum at 2, 4, 4, and 6 h in cultures grown in the presence of 0, 10, 100, and 1,000 mg of KNO₃/liter, respectively, while induction of nirS gene ranged from 12- to 225-fold compared to time zero. cnorB gene expression also followed a similar trend. nosZ gene expression did not respond to NO₃⁻ treatment under the conditions tested. Acetylene decreased nosZ gene expression but did not affect nirS or cnorB gene expression. These results showed that nirS and cnorB responded to nitrate concentrations; however, significant denitrification activity was only observed in culture with 1,000 mg of KNO₃/liter, indicating that there was no relationship between gene expression and denitrification activity under the conditions tested.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>19525277</pmid><doi>10.1128/AEM.00777-09</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetylene - metabolism Bacteria Bacterial Proteins - biosynthesis Chromatography Denitrification Effects Gene expression Gene Expression Profiling Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Microbiology Nitrates Nitrates - metabolism Physiology Pseudomonas Pseudomonas - growth & development Pseudomonas - metabolism |
| title | Effect of Nitrate and Acetylene on nirS, cnorB, and nosZ Expression and Denitrification Activity in Pseudomonas mandelii |
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