Development and validation of HPLC method for determination of sugars in palm sap, palm syrup, sugarcane jaggery and palm jaggery
A simple and reproducible method for the qualitative and quantitative analysis of free mono(fructose, glucose) and disaccharides (sucrose) using a high performance liquid chromatography (HPLC) with refractive index (RI) detector and a NH2 column was developed for matrices rich in monosaccharides (ho...
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| Veröffentlicht in: | International food research journal 2018-04, Vol.25 (2), p.649-654 |
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| container_title | International food research journal |
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| creator | Veena, K S Sameena, M T Padmakumari, A K P Srinivasa, G T K Nishanth, K S Reshma, M V |
| description | A simple and reproducible method for the qualitative and quantitative analysis of free mono(fructose, glucose) and disaccharides (sucrose) using a high performance liquid chromatography (HPLC) with refractive index (RI) detector and a NH2 column was developed for matrices rich in monosaccharides (honey) and disaccharides (palm sap, palm syrup, sugarcane jaggery, palm jaggery). The developed HPLC method was validated in terms of their linearity, limit of detection and quantification, precision and accuracy. Method validation studies showed that 85:15 (v/v) acetonitrile/water (CH3CN:H2O) was suitable for matrix containing monosaccharides and 65:35 (v/v) (CH3CN: H2O) was suitable for matrix containing disaccharides. The method showed good linearity with determination coefficients exceeding 0.99. Recovery studies indicated that 65% (CH3CN:H2O) system was suitable for matrix rich in sucrose (e.g. jaggery, syrup, etc.). |
| format | Article |
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The developed HPLC method was validated in terms of their linearity, limit of detection and quantification, precision and accuracy. Method validation studies showed that 85:15 (v/v) acetonitrile/water (CH3CN:H2O) was suitable for matrix containing monosaccharides and 65:35 (v/v) (CH3CN: H2O) was suitable for matrix containing disaccharides. The method showed good linearity with determination coefficients exceeding 0.99. Recovery studies indicated that 65% (CH3CN:H2O) system was suitable for matrix rich in sucrose (e.g. jaggery, syrup, etc.).</description><identifier>ISSN: 1985-4668</identifier><identifier>EISSN: 2231-7546</identifier><language>eng</language><publisher>Selangor: Universiti Putra Malaysia, Faculty of Food Science & Technology</publisher><subject>Carbohydrates ; Chromatography ; Food science ; Glucose ; High-performance liquid chromatography ; Honey ; Liquid chromatography ; Palm ; Sucrose ; Sugar ; Sugarcane ; Syrup</subject><ispartof>International food research journal, 2018-04, Vol.25 (2), p.649-654</ispartof><rights>Copyright Universiti Putra Malaysia, Faculty of Food Science & Technology 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Veena, K S</creatorcontrib><creatorcontrib>Sameena, M T</creatorcontrib><creatorcontrib>Padmakumari, A K P</creatorcontrib><creatorcontrib>Srinivasa, G T K</creatorcontrib><creatorcontrib>Nishanth, K S</creatorcontrib><creatorcontrib>Reshma, M V</creatorcontrib><title>Development and validation of HPLC method for determination of sugars in palm sap, palm syrup, sugarcane jaggery and palm jaggery</title><title>International food research journal</title><description>A simple and reproducible method for the qualitative and quantitative analysis of free mono(fructose, glucose) and disaccharides (sucrose) using a high performance liquid chromatography (HPLC) with refractive index (RI) detector and a NH2 column was developed for matrices rich in monosaccharides (honey) and disaccharides (palm sap, palm syrup, sugarcane jaggery, palm jaggery). The developed HPLC method was validated in terms of their linearity, limit of detection and quantification, precision and accuracy. Method validation studies showed that 85:15 (v/v) acetonitrile/water (CH3CN:H2O) was suitable for matrix containing monosaccharides and 65:35 (v/v) (CH3CN: H2O) was suitable for matrix containing disaccharides. The method showed good linearity with determination coefficients exceeding 0.99. Recovery studies indicated that 65% (CH3CN:H2O) system was suitable for matrix rich in sucrose (e.g. jaggery, syrup, etc.).</description><subject>Carbohydrates</subject><subject>Chromatography</subject><subject>Food science</subject><subject>Glucose</subject><subject>High-performance liquid chromatography</subject><subject>Honey</subject><subject>Liquid chromatography</subject><subject>Palm</subject><subject>Sucrose</subject><subject>Sugar</subject><subject>Sugarcane</subject><subject>Syrup</subject><issn>1985-4668</issn><issn>2231-7546</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNo9jU1LxDAYhIMoWNb9DwGvFtIkbZKj1I8VCnrQ85Ju3tSWNqlJu7BH_7llt3iah5lh5gollLIsFTkvrlGSKZmnvCjkLdrG2BFCMsZZQUWCfp_gCL0fB3AT1s7go-5bo6fWO-wt3n1UJR5g-vYGWx-wgQnC0Lr_QpwbHSJuHR51P-Cox4eVTmFe-JwftAPc6aaBcDqfnBurcYdurO4jbFfdoK-X589yl1bvr2_lY5WOmWRTKrWiICxwMMSqmivCiKTM1CQTteS5XUxFRU1BcwLcyvogTWa0yFlhSE7ZBt1fdsfgf2aI077zc3DL5Z6SXBFBlBLsD6gsXqg</recordid><startdate>20180401</startdate><enddate>20180401</enddate><creator>Veena, K S</creator><creator>Sameena, M T</creator><creator>Padmakumari, A K P</creator><creator>Srinivasa, G T K</creator><creator>Nishanth, K S</creator><creator>Reshma, M V</creator><general>Universiti Putra Malaysia, Faculty of Food Science & Technology</general><scope>7RQ</scope><scope>7XB</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BVBZV</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope><scope>Q9U</scope></search><sort><creationdate>20180401</creationdate><title>Development and validation of HPLC method for determination of sugars in palm sap, palm syrup, sugarcane jaggery and palm jaggery</title><author>Veena, K S ; 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The developed HPLC method was validated in terms of their linearity, limit of detection and quantification, precision and accuracy. Method validation studies showed that 85:15 (v/v) acetonitrile/water (CH3CN:H2O) was suitable for matrix containing monosaccharides and 65:35 (v/v) (CH3CN: H2O) was suitable for matrix containing disaccharides. The method showed good linearity with determination coefficients exceeding 0.99. Recovery studies indicated that 65% (CH3CN:H2O) system was suitable for matrix rich in sucrose (e.g. jaggery, syrup, etc.).</abstract><cop>Selangor</cop><pub>Universiti Putra Malaysia, Faculty of Food Science & Technology</pub><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 1985-4668 |
| ispartof | International food research journal, 2018-04, Vol.25 (2), p.649-654 |
| issn | 1985-4668 2231-7546 |
| language | eng |
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| source | EZB-FREE-00999 freely available EZB journals |
| subjects | Carbohydrates Chromatography Food science Glucose High-performance liquid chromatography Honey Liquid chromatography Palm Sucrose Sugar Sugarcane Syrup |
| title | Development and validation of HPLC method for determination of sugars in palm sap, palm syrup, sugarcane jaggery and palm jaggery |
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