Mitochondrial CO1 genetic marker-based species diversity of cuttlefish (Cephalopod; Mollusk) in Manado Bay and Lembeh Strait, North Sulawesi, Indonesia
This study determines cuttlefish species from Manado Bay and Lembeh Strait, North Sulawesi, based upon the mitochondrial CO1 genetic marker. Sample collection used SCUBA gear and fishermen catch. Seven cuttlefish specimens were collected in this study. DNA genomes of the samples were extracted, and...
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| Veröffentlicht in: | Aquaculture, Aquarium, Conservation & Legislation Aquarium, Conservation & Legislation, 2016-12, Vol.9 (6), p.1345-1354 |
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| creator | Pratasik, Silvester B Marsoedi Arfiati, Diana Setyohadi, Daduk |
| description | This study determines cuttlefish species from Manado Bay and Lembeh Strait, North Sulawesi, based upon the mitochondrial CO1 genetic marker. Sample collection used SCUBA gear and fishermen catch. Seven cuttlefish specimens were collected in this study. DNA genomes of the samples were extracted, and CO1 gene fragments were amplified through PCR and sequenced using Big Dye© terminator chemistry (Perkin Elmer). Species identification was conducted with BLAST method. Results showed that there were four species of cuttlefish in Manado Bay and Lembeh Strait, Sepia latimanus, S. pharaonis, Metasepia g, and Sepia ramani. S. latimanus was represented by 4 individuals, and the rests by only one individual. Phylogenetic analysis revealed that all samples were separated into 4 species groups, SP1, SP3. SP4 and SP10 (group1), SP5 (group2), SP6 (group3) and SP11 (group4). All species belong to important fisheries resources, but they are in insufficient data status, both fishing rate and population level. |
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Sample collection used SCUBA gear and fishermen catch. Seven cuttlefish specimens were collected in this study. DNA genomes of the samples were extracted, and CO1 gene fragments were amplified through PCR and sequenced using Big Dye© terminator chemistry (Perkin Elmer). Species identification was conducted with BLAST method. Results showed that there were four species of cuttlefish in Manado Bay and Lembeh Strait, Sepia latimanus, S. pharaonis, Metasepia g, and Sepia ramani. S. latimanus was represented by 4 individuals, and the rests by only one individual. Phylogenetic analysis revealed that all samples were separated into 4 species groups, SP1, SP3. SP4 and SP10 (group1), SP5 (group2), SP6 (group3) and SP11 (group4). All species belong to important fisheries resources, but they are in insufficient data status, both fishing rate and population level.</description><identifier>ISSN: 1844-8143</identifier><identifier>EISSN: 1844-9166</identifier><language>eng</language><publisher>Cluj-Napoca: Bioflux SRL</publisher><subject>Animal behavior ; Biodiversity ; Cephalopod fisheries ; Cephalopoda ; Cytochrome ; Deoxyribonucleic acid ; DNA ; Dyes ; Endangered & extinct species ; Fisheries ; Fishers ; Fishery resources ; Fishing ; Genetic diversity ; Genetic markers ; Genomes ; Identification ; Idiosepiidae ; Idiosepius pygmaeus ; Marine biology ; Marine molluscs ; Mitochondria ; Morphology ; Nucleotide sequence ; Organic chemistry ; PCR ; Phylogenetics ; Phylogeny ; Scuba diving ; Sepia ; Sepia latimanus ; Sepia orbignyana ; Sepia pharaonis ; Sepiidae ; Sp1 protein ; Species diversity ; Species identification ; Studies ; Taxonomy</subject><ispartof>Aquaculture, Aquarium, Conservation & Legislation, 2016-12, Vol.9 (6), p.1345-1354</ispartof><rights>Copyright Bioflux SRL Dec 2016</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Pratasik, Silvester B</creatorcontrib><creatorcontrib>Marsoedi</creatorcontrib><creatorcontrib>Arfiati, Diana</creatorcontrib><creatorcontrib>Setyohadi, Daduk</creatorcontrib><title>Mitochondrial CO1 genetic marker-based species diversity of cuttlefish (Cephalopod; Mollusk) in Manado Bay and Lembeh Strait, North Sulawesi, Indonesia</title><title>Aquaculture, Aquarium, Conservation & Legislation</title><description>This study determines cuttlefish species from Manado Bay and Lembeh Strait, North Sulawesi, based upon the mitochondrial CO1 genetic marker. 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All species belong to important fisheries resources, but they are in insufficient data status, both fishing rate and population level.</description><subject>Animal behavior</subject><subject>Biodiversity</subject><subject>Cephalopod fisheries</subject><subject>Cephalopoda</subject><subject>Cytochrome</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Dyes</subject><subject>Endangered & extinct species</subject><subject>Fisheries</subject><subject>Fishers</subject><subject>Fishery resources</subject><subject>Fishing</subject><subject>Genetic diversity</subject><subject>Genetic markers</subject><subject>Genomes</subject><subject>Identification</subject><subject>Idiosepiidae</subject><subject>Idiosepius pygmaeus</subject><subject>Marine biology</subject><subject>Marine molluscs</subject><subject>Mitochondria</subject><subject>Morphology</subject><subject>Nucleotide sequence</subject><subject>Organic chemistry</subject><subject>PCR</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Scuba diving</subject><subject>Sepia</subject><subject>Sepia latimanus</subject><subject>Sepia orbignyana</subject><subject>Sepia pharaonis</subject><subject>Sepiidae</subject><subject>Sp1 protein</subject><subject>Species diversity</subject><subject>Species identification</subject><subject>Studies</subject><subject>Taxonomy</subject><issn>1844-8143</issn><issn>1844-9166</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNotjc1OwzAQhCMEEhX0HVbiAlIjxYnjJOIEFT-VWnoo92pjr4nb1A6xA-qT8LoE0ZnDfHOZOYsmrOQ8rpgQ5ycuGc8uo6n3u-RPxeh8Ev2sTHCycVb1BluYrxl8kKVgJByw31Mf1-hJge9IGvKgzBf13oQjOA1yCKElbXwDt3PqGmxd59Q9rFzbDn5_B8bCCi0qB494BLQKlnSoqYFN6NGEGby5PoxtaPGbvJnBwipnR8Lr6EJj62l6yqto8_z0Pn-Nl-uXxfxhGXdVGeKSa60EyhqTVAidcsbLJNWCV6VklUozRGKFEjUVeZKpAnMuhdQ1T2tJOcuuopv_1a53nwP5sN25obfj4TZN8qLiLOE8-wUWTWXC</recordid><startdate>20161230</startdate><enddate>20161230</enddate><creator>Pratasik, Silvester B</creator><creator>Marsoedi</creator><creator>Arfiati, Diana</creator><creator>Setyohadi, Daduk</creator><general>Bioflux SRL</general><scope>7TN</scope><scope>8FD</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H95</scope><scope>H97</scope><scope>H98</scope><scope>H99</scope><scope>HCIFZ</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope></search><sort><creationdate>20161230</creationdate><title>Mitochondrial CO1 genetic marker-based species diversity of cuttlefish (Cephalopod; 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Mollusk) in Manado Bay and Lembeh Strait, North Sulawesi, Indonesia</atitle><jtitle>Aquaculture, Aquarium, Conservation & Legislation</jtitle><date>2016-12-30</date><risdate>2016</risdate><volume>9</volume><issue>6</issue><spage>1345</spage><epage>1354</epage><pages>1345-1354</pages><issn>1844-8143</issn><eissn>1844-9166</eissn><abstract>This study determines cuttlefish species from Manado Bay and Lembeh Strait, North Sulawesi, based upon the mitochondrial CO1 genetic marker. Sample collection used SCUBA gear and fishermen catch. Seven cuttlefish specimens were collected in this study. DNA genomes of the samples were extracted, and CO1 gene fragments were amplified through PCR and sequenced using Big Dye© terminator chemistry (Perkin Elmer). Species identification was conducted with BLAST method. Results showed that there were four species of cuttlefish in Manado Bay and Lembeh Strait, Sepia latimanus, S. pharaonis, Metasepia g, and Sepia ramani. S. latimanus was represented by 4 individuals, and the rests by only one individual. Phylogenetic analysis revealed that all samples were separated into 4 species groups, SP1, SP3. SP4 and SP10 (group1), SP5 (group2), SP6 (group3) and SP11 (group4). All species belong to important fisheries resources, but they are in insufficient data status, both fishing rate and population level.</abstract><cop>Cluj-Napoca</cop><pub>Bioflux SRL</pub><tpages>10</tpages></addata></record> |
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| subjects | Animal behavior Biodiversity Cephalopod fisheries Cephalopoda Cytochrome Deoxyribonucleic acid DNA Dyes Endangered & extinct species Fisheries Fishers Fishery resources Fishing Genetic diversity Genetic markers Genomes Identification Idiosepiidae Idiosepius pygmaeus Marine biology Marine molluscs Mitochondria Morphology Nucleotide sequence Organic chemistry PCR Phylogenetics Phylogeny Scuba diving Sepia Sepia latimanus Sepia orbignyana Sepia pharaonis Sepiidae Sp1 protein Species diversity Species identification Studies Taxonomy |
| title | Mitochondrial CO1 genetic marker-based species diversity of cuttlefish (Cephalopod; Mollusk) in Manado Bay and Lembeh Strait, North Sulawesi, Indonesia |
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