Efficient in planta gene targeting in tomato using geminiviral replicons and the CRISPR/Cas9 system

Summary Current breeding relies mostly on random mutagenesis and recombination to generate novel genetic variation. However, targeted genome editing is becoming an increasingly important tool for precise plant breeding. Using the CRISPR‐Cas system combined with the bean yellow dwarf virus rolling ci...

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Veröffentlicht in:	The Plant journal : for cell and molecular biology 2018-07, Vol.95 (1), p.5-16
Hauptverfasser:	Dahan‐Meir, Tal, Filler‐Hayut, Shdema, Melamed‐Bessudo, Cathy, Bocobza, Samuel, Czosnek, Henryk, Aharoni, Asaph, Levy, Avraham A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Amplification Biosynthesis Carotenoids Change detection CRISPR CRISPR-Associated Protein 9 CRISPR-Cas Systems CRISPR‐Cas9 Deoxyribonucleic acid DNA DNA Breaks, Double-Stranded DNA damage Double-strand break repair geminiviral replicons Geminiviridae - genetics Gene Editing - methods gene replacement Gene targeting Gene Targeting - methods Genes, Plant - genetics Genetic diversity Genome editing Genomes High-Throughput Nucleotide Sequencing Homologous recombination Homology Lycopersicon esculentum - genetics Modular construction Mutagenesis Phenotypes Phytoene synthase Plant breeding Plants, Genetically Modified - genetics Random mutagenesis Replicon - genetics Site-directed mutagenesis Tomatoes Viruses
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