Detection of Pantoea ananatis in corn seeds on semi-selective medium
Maize white spot, caused by the bacterium Pantoea ananatis , is one of the main leaf diseases of maize in Brazil. However, until now, its presence in corn seeds has not been detected. In this study, P. ananatis was detected in corn seeds using a semi-selective culture medium. A bacterial suspension...
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| Veröffentlicht in: | Tropical Plant Pathology 2018-06, Vol.43 (3), p.254-256 |
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| creator | Mamede, Morgana C. Tebaldi, Nilvanira D. Mota, Lara C. B. M. Martins, Olinda M. Coelho, Lísias |
| description | Maize white spot, caused by the bacterium
Pantoea ananatis
, is one of the main leaf diseases of maize in Brazil. However, until now, its presence in corn seeds has not been detected. In this study,
P. ananatis
was detected in corn seeds using a semi-selective culture medium. A bacterial suspension of
P. ananatis
was serially diluted ten-fold and plated on 523, YDC or semi-selective PA20 media, with or without the addition of methyl thiophanate. The detection of bacteria in seeds was evaluated in three seed lot samples, collected from field maize plants with white spot symptoms. Seed washings were prepared, ten-fold serially diluted and plated on the same three culture media. Addition of methyl thiophanate to 532, YDC and PA20 culture media did not inhibit bacterial growth.
Pantoea ananatis
was detected in two corn seed samples on YDC and PA20 media, which was confirmed by biochemical, physiological and molecular characterization as well as by pathogenicity tests. This is the first report of
P. ananatis
detection in corn seeds. |
| doi_str_mv | 10.1007/s40858-017-0203-z |
| format | Article |
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Pantoea ananatis
, is one of the main leaf diseases of maize in Brazil. However, until now, its presence in corn seeds has not been detected. In this study,
P. ananatis
was detected in corn seeds using a semi-selective culture medium. A bacterial suspension of
P. ananatis
was serially diluted ten-fold and plated on 523, YDC or semi-selective PA20 media, with or without the addition of methyl thiophanate. The detection of bacteria in seeds was evaluated in three seed lot samples, collected from field maize plants with white spot symptoms. Seed washings were prepared, ten-fold serially diluted and plated on the same three culture media. Addition of methyl thiophanate to 532, YDC and PA20 culture media did not inhibit bacterial growth.
Pantoea ananatis
was detected in two corn seed samples on YDC and PA20 media, which was confirmed by biochemical, physiological and molecular characterization as well as by pathogenicity tests. This is the first report of
P. ananatis
detection in corn seeds.</description><identifier>ISSN: 1983-2052</identifier><identifier>ISSN: 1982-5676</identifier><identifier>EISSN: 1983-2052</identifier><identifier>DOI: 10.1007/s40858-017-0203-z</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Bacteria ; Biomedical and Life Sciences ; Corn ; Culture media ; Dilution ; Life Sciences ; Molecular chains ; Pantoea ; Pathogenicity ; Pathogens ; Plant Pathology ; Seeds ; Selective media ; Short Communication ; Thiophanate</subject><ispartof>Tropical Plant Pathology, 2018-06, Vol.43 (3), p.254-256</ispartof><rights>Sociedade Brasileira de Fitopatologia 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Copyright Springer Science & Business Media 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-ac37b09a81fc0159624a79dde823b4b1e6ede9672b8098ae1946e1bb56b527c13</citedby><cites>FETCH-LOGICAL-c398t-ac37b09a81fc0159624a79dde823b4b1e6ede9672b8098ae1946e1bb56b527c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s40858-017-0203-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s40858-017-0203-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Mamede, Morgana C.</creatorcontrib><creatorcontrib>Tebaldi, Nilvanira D.</creatorcontrib><creatorcontrib>Mota, Lara C. B. M.</creatorcontrib><creatorcontrib>Martins, Olinda M.</creatorcontrib><creatorcontrib>Coelho, Lísias</creatorcontrib><title>Detection of Pantoea ananatis in corn seeds on semi-selective medium</title><title>Tropical Plant Pathology</title><addtitle>Trop. plant pathol</addtitle><description>Maize white spot, caused by the bacterium
Pantoea ananatis
, is one of the main leaf diseases of maize in Brazil. However, until now, its presence in corn seeds has not been detected. In this study,
P. ananatis
was detected in corn seeds using a semi-selective culture medium. A bacterial suspension of
P. ananatis
was serially diluted ten-fold and plated on 523, YDC or semi-selective PA20 media, with or without the addition of methyl thiophanate. The detection of bacteria in seeds was evaluated in three seed lot samples, collected from field maize plants with white spot symptoms. Seed washings were prepared, ten-fold serially diluted and plated on the same three culture media. Addition of methyl thiophanate to 532, YDC and PA20 culture media did not inhibit bacterial growth.
Pantoea ananatis
was detected in two corn seed samples on YDC and PA20 media, which was confirmed by biochemical, physiological and molecular characterization as well as by pathogenicity tests. This is the first report of
P. ananatis
detection in corn seeds.</description><subject>Bacteria</subject><subject>Biomedical and Life Sciences</subject><subject>Corn</subject><subject>Culture media</subject><subject>Dilution</subject><subject>Life Sciences</subject><subject>Molecular chains</subject><subject>Pantoea</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Plant Pathology</subject><subject>Seeds</subject><subject>Selective media</subject><subject>Short Communication</subject><subject>Thiophanate</subject><issn>1983-2052</issn><issn>1982-5676</issn><issn>1983-2052</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kE1LxDAQhosouK7-AG8Fz9FJ0jbJcdn1Cxb0oOeQptOlyzZZk67g_npTKuhF5jDD8D7z8WbZNYVbCiDuYgGylASoIMCAk-NJNqNKcsKgZKd_6vPsIsYtQMVUJWfZaoUD2qHzLvdt_mrc4NHkxqUYuph3Lrc-uDwiNjH3Y9F3JOJuZD4x77HpDv1ldtaaXcSrnzzP3h_u35ZPZP3y-LxcrInlSg7EWC5qUEbS1gItVcUKI1TToGS8LmqKFTaoKsFqCUoapKqokNZ1WdUlE5byeXYzzd0H_3HAOOitPwSXVur0Gi84B4Ckup1UG7ND3bnWD8HYFE263XqHbZf6CwGiAMFKlgA6ATb4GAO2eh-63oQvTUGP7urJXZ3c1aO7-pgYNjExad0Gw-8p_0PfnIh8KQ</recordid><startdate>20180601</startdate><enddate>20180601</enddate><creator>Mamede, Morgana C.</creator><creator>Tebaldi, Nilvanira D.</creator><creator>Mota, Lara C. B. M.</creator><creator>Martins, Olinda M.</creator><creator>Coelho, Lísias</creator><general>Springer International Publishing</general><general>Springer</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>IAO</scope></search><sort><creationdate>20180601</creationdate><title>Detection of Pantoea ananatis in corn seeds on semi-selective medium</title><author>Mamede, Morgana C. ; Tebaldi, Nilvanira D. ; Mota, Lara C. B. M. ; Martins, Olinda M. ; Coelho, Lísias</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-ac37b09a81fc0159624a79dde823b4b1e6ede9672b8098ae1946e1bb56b527c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Bacteria</topic><topic>Biomedical and Life Sciences</topic><topic>Corn</topic><topic>Culture media</topic><topic>Dilution</topic><topic>Life Sciences</topic><topic>Molecular chains</topic><topic>Pantoea</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Plant Pathology</topic><topic>Seeds</topic><topic>Selective media</topic><topic>Short Communication</topic><topic>Thiophanate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mamede, Morgana C.</creatorcontrib><creatorcontrib>Tebaldi, Nilvanira D.</creatorcontrib><creatorcontrib>Mota, Lara C. B. M.</creatorcontrib><creatorcontrib>Martins, Olinda M.</creatorcontrib><creatorcontrib>Coelho, Lísias</creatorcontrib><collection>CrossRef</collection><collection>Gale Academic OneFile</collection><jtitle>Tropical Plant Pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mamede, Morgana C.</au><au>Tebaldi, Nilvanira D.</au><au>Mota, Lara C. B. M.</au><au>Martins, Olinda M.</au><au>Coelho, Lísias</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Pantoea ananatis in corn seeds on semi-selective medium</atitle><jtitle>Tropical Plant Pathology</jtitle><stitle>Trop. plant pathol</stitle><date>2018-06-01</date><risdate>2018</risdate><volume>43</volume><issue>3</issue><spage>254</spage><epage>256</epage><pages>254-256</pages><issn>1983-2052</issn><issn>1982-5676</issn><eissn>1983-2052</eissn><abstract>Maize white spot, caused by the bacterium
Pantoea ananatis
, is one of the main leaf diseases of maize in Brazil. However, until now, its presence in corn seeds has not been detected. In this study,
P. ananatis
was detected in corn seeds using a semi-selective culture medium. A bacterial suspension of
P. ananatis
was serially diluted ten-fold and plated on 523, YDC or semi-selective PA20 media, with or without the addition of methyl thiophanate. The detection of bacteria in seeds was evaluated in three seed lot samples, collected from field maize plants with white spot symptoms. Seed washings were prepared, ten-fold serially diluted and plated on the same three culture media. Addition of methyl thiophanate to 532, YDC and PA20 culture media did not inhibit bacterial growth.
Pantoea ananatis
was detected in two corn seed samples on YDC and PA20 media, which was confirmed by biochemical, physiological and molecular characterization as well as by pathogenicity tests. This is the first report of
P. ananatis
detection in corn seeds.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s40858-017-0203-z</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Tropical Plant Pathology, 2018-06, Vol.43 (3), p.254-256 |
| issn | 1983-2052 1982-5676 1983-2052 |
| language | eng |
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| subjects | Bacteria Biomedical and Life Sciences Corn Culture media Dilution Life Sciences Molecular chains Pantoea Pathogenicity Pathogens Plant Pathology Seeds Selective media Short Communication Thiophanate |
| title | Detection of Pantoea ananatis in corn seeds on semi-selective medium |
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