The influence of lulo (Solanum quitoense Lam) fruit maturity stage on polygalacturonase and pectate lyase secretion by Colletotrichum acutatum
The influence of the lulo fruit maturity stage on the secretion of polygalacturonase (PG) and pectate lyase (PL) enzymes by Colletotrichum acutatum was determined. The study was performed using in vitro assays in the presence of exocarp and cell walls (CW) of the fruit at three different ripening st...
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| Veröffentlicht in: | Tropical Plant Pathology 2018-06, Vol.43 (3), p.218-229 |
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| container_title | Tropical Plant Pathology |
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| creator | Martínez-González, Ana Patricia Higuera-Mancipe, Blanca Ligia Martínez-Peralta, Sixta Tulia |
| description | The influence of the lulo fruit maturity stage on the secretion of polygalacturonase (PG) and pectate lyase (PL) enzymes by
Colletotrichum acutatum
was determined. The study was performed using
in vitro
assays in the presence of exocarp and cell walls (CW) of the fruit at three different ripening stages (unripe, semi-ripe and ripe). The results showed that PG behaves as a constitutive enzyme and is only induced by semi-ripe CW, while PL is induced with the exocarp of lulo fruits at the three maturation stages, although its activity was highest with semi-ripe fruit. This enzymatic behavior might be related to the quiescent stage of the pathogen in the fruit, as
C. acutatum
remains quiescent until maturation begins. The profiles of PL induction were different in the presence of the CW and the exocarp inducers, whereas PG levels barely varied, indicating that the process of obtaining the CWs may eliminate a compound that contributes to the induction of PL secretion. |
| doi_str_mv | 10.1007/s40858-017-0209-6 |
| format | Article |
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Colletotrichum acutatum
was determined. The study was performed using
in vitro
assays in the presence of exocarp and cell walls (CW) of the fruit at three different ripening stages (unripe, semi-ripe and ripe). The results showed that PG behaves as a constitutive enzyme and is only induced by semi-ripe CW, while PL is induced with the exocarp of lulo fruits at the three maturation stages, although its activity was highest with semi-ripe fruit. This enzymatic behavior might be related to the quiescent stage of the pathogen in the fruit, as
C. acutatum
remains quiescent until maturation begins. The profiles of PL induction were different in the presence of the CW and the exocarp inducers, whereas PG levels barely varied, indicating that the process of obtaining the CWs may eliminate a compound that contributes to the induction of PL secretion.</description><identifier>ISSN: 1983-2052</identifier><identifier>ISSN: 1982-5676</identifier><identifier>EISSN: 1983-2052</identifier><identifier>DOI: 10.1007/s40858-017-0209-6</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Biomedical and Life Sciences ; Cell walls ; Colletotrichum acutatum ; Developmental biology ; Enzymes ; Ethylenediaminetetraacetic acid ; Fruit ; Fruits ; Life Sciences ; Maturation ; Original Article ; Pectate lyase ; Plant Pathology ; Polygalacturonase ; Ripening ; Secretion</subject><ispartof>Tropical Plant Pathology, 2018-06, Vol.43 (3), p.218-229</ispartof><rights>Sociedade Brasileira de Fitopatologia 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Copyright Springer Science & Business Media 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-62ac7d35475ab8977c2e1576ea34793a19e2167a511edbd22fee98bad67d8ec73</citedby><cites>FETCH-LOGICAL-c398t-62ac7d35475ab8977c2e1576ea34793a19e2167a511edbd22fee98bad67d8ec73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s40858-017-0209-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s40858-017-0209-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27913,27914,41477,42546,51308</link.rule.ids></links><search><creatorcontrib>Martínez-González, Ana Patricia</creatorcontrib><creatorcontrib>Higuera-Mancipe, Blanca Ligia</creatorcontrib><creatorcontrib>Martínez-Peralta, Sixta Tulia</creatorcontrib><title>The influence of lulo (Solanum quitoense Lam) fruit maturity stage on polygalacturonase and pectate lyase secretion by Colletotrichum acutatum</title><title>Tropical Plant Pathology</title><addtitle>Trop. plant pathol</addtitle><description>The influence of the lulo fruit maturity stage on the secretion of polygalacturonase (PG) and pectate lyase (PL) enzymes by
Colletotrichum acutatum
was determined. The study was performed using
in vitro
assays in the presence of exocarp and cell walls (CW) of the fruit at three different ripening stages (unripe, semi-ripe and ripe). The results showed that PG behaves as a constitutive enzyme and is only induced by semi-ripe CW, while PL is induced with the exocarp of lulo fruits at the three maturation stages, although its activity was highest with semi-ripe fruit. This enzymatic behavior might be related to the quiescent stage of the pathogen in the fruit, as
C. acutatum
remains quiescent until maturation begins. The profiles of PL induction were different in the presence of the CW and the exocarp inducers, whereas PG levels barely varied, indicating that the process of obtaining the CWs may eliminate a compound that contributes to the induction of PL secretion.</description><subject>Biomedical and Life Sciences</subject><subject>Cell walls</subject><subject>Colletotrichum acutatum</subject><subject>Developmental biology</subject><subject>Enzymes</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Fruit</subject><subject>Fruits</subject><subject>Life Sciences</subject><subject>Maturation</subject><subject>Original Article</subject><subject>Pectate lyase</subject><subject>Plant Pathology</subject><subject>Polygalacturonase</subject><subject>Ripening</subject><subject>Secretion</subject><issn>1983-2052</issn><issn>1982-5676</issn><issn>1983-2052</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kbtu3DAQRYUgBuI4_oB0BNIkhRySepAqjUUeBhZIEbsmZqnRWgZFrvko9BP5Zs9CAZLGYEFyeM_McG5VfRT8RnCuvqaW607XXKiaSz7U_ZvqUgy6qSXv5Nv_zu-q9yk9cd7LodeX1Z_7R2Szn1xBb5GFibniAvv8OzjwZWHPZc4BfUK2h-ULmyLd2QK5xDmvLGU4EuTZKbj1CA4sPQQPJAc_shPaDBmZW8-RhDZinkl9WNkuOIc55DjbRyoDtpCyLB-qiwlcwuu_-1X18P3b_e5nvf_14253u69tM-hc9xKsGpuuVR0c9KCUlSg61SM0rRoaEANK0SvohMDxMEo5IQ76AGOvRo1WNVfVpy3vKYbngimbp1Cip5KGhtS0jdRdQ6qbTUVfQ0NTon7B0hpxmW3wOM0Uv1VctVzJriVAbICNIaWIkznFeYG4GsHN2Sez-WTIJ3P2yfTEyI1JpPVHjP9aeR16AYOQmG0</recordid><startdate>20180601</startdate><enddate>20180601</enddate><creator>Martínez-González, Ana Patricia</creator><creator>Higuera-Mancipe, Blanca Ligia</creator><creator>Martínez-Peralta, Sixta Tulia</creator><general>Springer International Publishing</general><general>Springer</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>IAO</scope></search><sort><creationdate>20180601</creationdate><title>The influence of lulo (Solanum quitoense Lam) fruit maturity stage on polygalacturonase and pectate lyase secretion by Colletotrichum acutatum</title><author>Martínez-González, Ana Patricia ; Higuera-Mancipe, Blanca Ligia ; Martínez-Peralta, Sixta Tulia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-62ac7d35475ab8977c2e1576ea34793a19e2167a511edbd22fee98bad67d8ec73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Biomedical and Life Sciences</topic><topic>Cell walls</topic><topic>Colletotrichum acutatum</topic><topic>Developmental biology</topic><topic>Enzymes</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Fruit</topic><topic>Fruits</topic><topic>Life Sciences</topic><topic>Maturation</topic><topic>Original Article</topic><topic>Pectate lyase</topic><topic>Plant Pathology</topic><topic>Polygalacturonase</topic><topic>Ripening</topic><topic>Secretion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martínez-González, Ana Patricia</creatorcontrib><creatorcontrib>Higuera-Mancipe, Blanca Ligia</creatorcontrib><creatorcontrib>Martínez-Peralta, Sixta Tulia</creatorcontrib><collection>CrossRef</collection><collection>Gale Academic OneFile</collection><jtitle>Tropical Plant Pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martínez-González, Ana Patricia</au><au>Higuera-Mancipe, Blanca Ligia</au><au>Martínez-Peralta, Sixta Tulia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The influence of lulo (Solanum quitoense Lam) fruit maturity stage on polygalacturonase and pectate lyase secretion by Colletotrichum acutatum</atitle><jtitle>Tropical Plant Pathology</jtitle><stitle>Trop. plant pathol</stitle><date>2018-06-01</date><risdate>2018</risdate><volume>43</volume><issue>3</issue><spage>218</spage><epage>229</epage><pages>218-229</pages><issn>1983-2052</issn><issn>1982-5676</issn><eissn>1983-2052</eissn><abstract>The influence of the lulo fruit maturity stage on the secretion of polygalacturonase (PG) and pectate lyase (PL) enzymes by
Colletotrichum acutatum
was determined. The study was performed using
in vitro
assays in the presence of exocarp and cell walls (CW) of the fruit at three different ripening stages (unripe, semi-ripe and ripe). The results showed that PG behaves as a constitutive enzyme and is only induced by semi-ripe CW, while PL is induced with the exocarp of lulo fruits at the three maturation stages, although its activity was highest with semi-ripe fruit. This enzymatic behavior might be related to the quiescent stage of the pathogen in the fruit, as
C. acutatum
remains quiescent until maturation begins. The profiles of PL induction were different in the presence of the CW and the exocarp inducers, whereas PG levels barely varied, indicating that the process of obtaining the CWs may eliminate a compound that contributes to the induction of PL secretion.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s40858-017-0209-6</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1983-2052 |
| ispartof | Tropical Plant Pathology, 2018-06, Vol.43 (3), p.218-229 |
| issn | 1983-2052 1982-5676 1983-2052 |
| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Biomedical and Life Sciences Cell walls Colletotrichum acutatum Developmental biology Enzymes Ethylenediaminetetraacetic acid Fruit Fruits Life Sciences Maturation Original Article Pectate lyase Plant Pathology Polygalacturonase Ripening Secretion |
| title | The influence of lulo (Solanum quitoense Lam) fruit maturity stage on polygalacturonase and pectate lyase secretion by Colletotrichum acutatum |
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