Development and validation of LC/APCI-MS method for the quantification of oat ceramides in skin permeation studies
Ceramides (CERs) are the backbone of the intercellular lipid lamellae of the stratum corneum (SC), the outer layer of the skin. Skin diseases such as atopic dermatitis, psoriasis, and aged skin are characterized by dysfunctional skin barrier and dryness which are associated with reduced levels of CE...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2018-08, Vol.410 (20), p.4775-4785 |
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| creator | Tessema, Efrem N. Gebre-Mariam, Tsige Frolov, Andrej Wohlrab, Johannes Neubert, Reinhard H. H. |
| description | Ceramides (CERs) are the backbone of the intercellular lipid lamellae of the
stratum corneum
(SC), the outer layer of the skin. Skin diseases such as atopic dermatitis, psoriasis, and aged skin are characterized by dysfunctional skin barrier and dryness which are associated with reduced levels of CERs. Replenishing the depleted epidermal CERs with exogenous CERs has been shown to have beneficial effects in improving the skin barrier and hydration. The exogenous CERs such as phyto-derived CERs (PhytoCERs) can be delivered deep into the SC using novel topical formulations. This, however, requires investigating the rate and extent of skin permeation of CERs. In this study, an LC/APCI-MS method to detect and quantify PhytoCERs in different layers of the skin has been developed and validated. The method was used to investigate the skin permeation of PhytoCERs using Franz diffusion cells after applying an amphiphilic cream containing PhytoCERs to the surface of ex vivo human skin. As plant-specific CERs are not commercially available, well-characterized CERs isolated from oat (
Avena abyssinica
) were used as reference standards for the development and validation of the method. The method was linear over the range of 30–1050 ng/mL and sensitive with limit of detection and quantification of 10 and 30 ng/mL, respectively. The method was also selective, accurate, and precise with minimal matrix effect (with mean matrix factor around 100%). Even if more than 85% of oat CERs in the cream remained in the cream after the incubation periods of 30, 100, and 300 min, it was possible to quantify the small quantities of oat CERs distributed across the SC, epidermis, and dermis of the skin indicating the method’s sensitivity. Therefore, the method can be used to investigate the skin permeation of oat CERs from the various pharmaceutical and cosmeceutical products without any interference from the skin constituents such as the epidermal lipids.
Graphical abstract
ᅟ |
| doi_str_mv | 10.1007/s00216-018-1162-z |
| format | Article |
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stratum corneum
(SC), the outer layer of the skin. Skin diseases such as atopic dermatitis, psoriasis, and aged skin are characterized by dysfunctional skin barrier and dryness which are associated with reduced levels of CERs. Replenishing the depleted epidermal CERs with exogenous CERs has been shown to have beneficial effects in improving the skin barrier and hydration. The exogenous CERs such as phyto-derived CERs (PhytoCERs) can be delivered deep into the SC using novel topical formulations. This, however, requires investigating the rate and extent of skin permeation of CERs. In this study, an LC/APCI-MS method to detect and quantify PhytoCERs in different layers of the skin has been developed and validated. The method was used to investigate the skin permeation of PhytoCERs using Franz diffusion cells after applying an amphiphilic cream containing PhytoCERs to the surface of ex vivo human skin. As plant-specific CERs are not commercially available, well-characterized CERs isolated from oat (
Avena abyssinica
) were used as reference standards for the development and validation of the method. The method was linear over the range of 30–1050 ng/mL and sensitive with limit of detection and quantification of 10 and 30 ng/mL, respectively. The method was also selective, accurate, and precise with minimal matrix effect (with mean matrix factor around 100%). Even if more than 85% of oat CERs in the cream remained in the cream after the incubation periods of 30, 100, and 300 min, it was possible to quantify the small quantities of oat CERs distributed across the SC, epidermis, and dermis of the skin indicating the method’s sensitivity. Therefore, the method can be used to investigate the skin permeation of oat CERs from the various pharmaceutical and cosmeceutical products without any interference from the skin constituents such as the epidermal lipids.
Graphical abstract
ᅟ</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-018-1162-z</identifier><identifier>PMID: 29947900</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analytical Chemistry ; Atopic dermatitis ; Avena - chemistry ; Biochemistry ; Ceramides ; Ceramides - analysis ; Ceramides - pharmacokinetics ; Characterization and Evaluation of Materials ; Chemical properties ; Chemistry ; Chemistry and Materials Science ; Chromatography, High Pressure Liquid - methods ; Communication ; Cosmetics industry ; Cream ; Dermatitis ; Dermis ; Diffusion cells ; Epidermis ; Food Science ; Formulations ; Glycine max - chemistry ; Humans ; Laboratory Medicine ; Lamellae ; Limit of Detection ; Lipids ; Liquid chromatography ; Mass spectrometry ; Mass Spectrometry - methods ; Measurement ; Methods ; Monitoring/Environmental Analysis ; Nutritional aspects ; Oats ; Penetration ; Psoriasis ; Skin ; Skin - metabolism ; Skin Absorption ; Skin diseases ; Stratum corneum</subject><ispartof>Analytical and bioanalytical chemistry, 2018-08, Vol.410 (20), p.4775-4785</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Analytical and Bioanalytical Chemistry is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-76d87267316b6fd791402b57942ed1cd0a2130e16563870b0fb62421ec1e77533</citedby><cites>FETCH-LOGICAL-c448t-76d87267316b6fd791402b57942ed1cd0a2130e16563870b0fb62421ec1e77533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-018-1162-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-018-1162-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29947900$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tessema, Efrem N.</creatorcontrib><creatorcontrib>Gebre-Mariam, Tsige</creatorcontrib><creatorcontrib>Frolov, Andrej</creatorcontrib><creatorcontrib>Wohlrab, Johannes</creatorcontrib><creatorcontrib>Neubert, Reinhard H. H.</creatorcontrib><title>Development and validation of LC/APCI-MS method for the quantification of oat ceramides in skin permeation studies</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Ceramides (CERs) are the backbone of the intercellular lipid lamellae of the
stratum corneum
(SC), the outer layer of the skin. Skin diseases such as atopic dermatitis, psoriasis, and aged skin are characterized by dysfunctional skin barrier and dryness which are associated with reduced levels of CERs. Replenishing the depleted epidermal CERs with exogenous CERs has been shown to have beneficial effects in improving the skin barrier and hydration. The exogenous CERs such as phyto-derived CERs (PhytoCERs) can be delivered deep into the SC using novel topical formulations. This, however, requires investigating the rate and extent of skin permeation of CERs. In this study, an LC/APCI-MS method to detect and quantify PhytoCERs in different layers of the skin has been developed and validated. The method was used to investigate the skin permeation of PhytoCERs using Franz diffusion cells after applying an amphiphilic cream containing PhytoCERs to the surface of ex vivo human skin. As plant-specific CERs are not commercially available, well-characterized CERs isolated from oat (
Avena abyssinica
) were used as reference standards for the development and validation of the method. The method was linear over the range of 30–1050 ng/mL and sensitive with limit of detection and quantification of 10 and 30 ng/mL, respectively. The method was also selective, accurate, and precise with minimal matrix effect (with mean matrix factor around 100%). Even if more than 85% of oat CERs in the cream remained in the cream after the incubation periods of 30, 100, and 300 min, it was possible to quantify the small quantities of oat CERs distributed across the SC, epidermis, and dermis of the skin indicating the method’s sensitivity. Therefore, the method can be used to investigate the skin permeation of oat CERs from the various pharmaceutical and cosmeceutical products without any interference from the skin constituents such as the epidermal lipids.
Graphical abstract
ᅟ</description><subject>Analytical Chemistry</subject><subject>Atopic dermatitis</subject><subject>Avena - chemistry</subject><subject>Biochemistry</subject><subject>Ceramides</subject><subject>Ceramides - analysis</subject><subject>Ceramides - pharmacokinetics</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemical properties</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Communication</subject><subject>Cosmetics industry</subject><subject>Cream</subject><subject>Dermatitis</subject><subject>Dermis</subject><subject>Diffusion cells</subject><subject>Epidermis</subject><subject>Food Science</subject><subject>Formulations</subject><subject>Glycine max - chemistry</subject><subject>Humans</subject><subject>Laboratory Medicine</subject><subject>Lamellae</subject><subject>Limit of Detection</subject><subject>Lipids</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass Spectrometry - methods</subject><subject>Measurement</subject><subject>Methods</subject><subject>Monitoring/Environmental Analysis</subject><subject>Nutritional aspects</subject><subject>Oats</subject><subject>Penetration</subject><subject>Psoriasis</subject><subject>Skin</subject><subject>Skin - metabolism</subject><subject>Skin Absorption</subject><subject>Skin diseases</subject><subject>Stratum corneum</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kVFvFCEUhSdGY2v1B_hiSHye9l5mBmYeN2vVJms0UZ8JM1xa6g5sgWlif700U9cnQwIX-M7hhlNVbxHOEUBeJACOogbsa0TB64dn1SmKsuOig-fHuuUn1auUbgGw61G8rE74MLRyADit4ge6p304zOQz096we713RmcXPAuW7bYXm2_bq_rLdzZTvgmG2RBZviF2t2ifnXXTkQ06s4minp2hxJxn6VeZDhRnWpmUF-Mova5eWL1P9OZpPat-frz8sf1c775-utpudvXUtn2upTC95EI2KEZhjRywBT52cmg5GZwMaI4NEIpONL2EEewoeMuRJiQpu6Y5q96vvocY7hZKWd2GJfrypOLQlW_pGi4Ldb5S13pPynkbctRTGYZmNwVP1pXzTdeKhhd7UQS4CqYYUopk1SG6WcffCkE9xqLWWFSJRT3Goh6K5t1TK8s4kzkq_uZQAL4CqVz5a4r_ev2_6x-rLZbW</recordid><startdate>20180801</startdate><enddate>20180801</enddate><creator>Tessema, Efrem N.</creator><creator>Gebre-Mariam, Tsige</creator><creator>Frolov, Andrej</creator><creator>Wohlrab, Johannes</creator><creator>Neubert, Reinhard H. 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H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-76d87267316b6fd791402b57942ed1cd0a2130e16563870b0fb62421ec1e77533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Analytical Chemistry</topic><topic>Atopic dermatitis</topic><topic>Avena - chemistry</topic><topic>Biochemistry</topic><topic>Ceramides</topic><topic>Ceramides - analysis</topic><topic>Ceramides - pharmacokinetics</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemical properties</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Communication</topic><topic>Cosmetics industry</topic><topic>Cream</topic><topic>Dermatitis</topic><topic>Dermis</topic><topic>Diffusion cells</topic><topic>Epidermis</topic><topic>Food Science</topic><topic>Formulations</topic><topic>Glycine max - chemistry</topic><topic>Humans</topic><topic>Laboratory Medicine</topic><topic>Lamellae</topic><topic>Limit of Detection</topic><topic>Lipids</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Mass Spectrometry - methods</topic><topic>Measurement</topic><topic>Methods</topic><topic>Monitoring/Environmental Analysis</topic><topic>Nutritional aspects</topic><topic>Oats</topic><topic>Penetration</topic><topic>Psoriasis</topic><topic>Skin</topic><topic>Skin - metabolism</topic><topic>Skin Absorption</topic><topic>Skin diseases</topic><topic>Stratum corneum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tessema, Efrem N.</creatorcontrib><creatorcontrib>Gebre-Mariam, Tsige</creatorcontrib><creatorcontrib>Frolov, Andrej</creatorcontrib><creatorcontrib>Wohlrab, Johannes</creatorcontrib><creatorcontrib>Neubert, Reinhard H. 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H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and validation of LC/APCI-MS method for the quantification of oat ceramides in skin permeation studies</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2018-08-01</date><risdate>2018</risdate><volume>410</volume><issue>20</issue><spage>4775</spage><epage>4785</epage><pages>4775-4785</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Ceramides (CERs) are the backbone of the intercellular lipid lamellae of the
stratum corneum
(SC), the outer layer of the skin. Skin diseases such as atopic dermatitis, psoriasis, and aged skin are characterized by dysfunctional skin barrier and dryness which are associated with reduced levels of CERs. Replenishing the depleted epidermal CERs with exogenous CERs has been shown to have beneficial effects in improving the skin barrier and hydration. The exogenous CERs such as phyto-derived CERs (PhytoCERs) can be delivered deep into the SC using novel topical formulations. This, however, requires investigating the rate and extent of skin permeation of CERs. In this study, an LC/APCI-MS method to detect and quantify PhytoCERs in different layers of the skin has been developed and validated. The method was used to investigate the skin permeation of PhytoCERs using Franz diffusion cells after applying an amphiphilic cream containing PhytoCERs to the surface of ex vivo human skin. As plant-specific CERs are not commercially available, well-characterized CERs isolated from oat (
Avena abyssinica
) were used as reference standards for the development and validation of the method. The method was linear over the range of 30–1050 ng/mL and sensitive with limit of detection and quantification of 10 and 30 ng/mL, respectively. The method was also selective, accurate, and precise with minimal matrix effect (with mean matrix factor around 100%). Even if more than 85% of oat CERs in the cream remained in the cream after the incubation periods of 30, 100, and 300 min, it was possible to quantify the small quantities of oat CERs distributed across the SC, epidermis, and dermis of the skin indicating the method’s sensitivity. Therefore, the method can be used to investigate the skin permeation of oat CERs from the various pharmaceutical and cosmeceutical products without any interference from the skin constituents such as the epidermal lipids.
Graphical abstract
ᅟ</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29947900</pmid><doi>10.1007/s00216-018-1162-z</doi><tpages>11</tpages></addata></record> |
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| subjects | Analytical Chemistry Atopic dermatitis Avena - chemistry Biochemistry Ceramides Ceramides - analysis Ceramides - pharmacokinetics Characterization and Evaluation of Materials Chemical properties Chemistry Chemistry and Materials Science Chromatography, High Pressure Liquid - methods Communication Cosmetics industry Cream Dermatitis Dermis Diffusion cells Epidermis Food Science Formulations Glycine max - chemistry Humans Laboratory Medicine Lamellae Limit of Detection Lipids Liquid chromatography Mass spectrometry Mass Spectrometry - methods Measurement Methods Monitoring/Environmental Analysis Nutritional aspects Oats Penetration Psoriasis Skin Skin - metabolism Skin Absorption Skin diseases Stratum corneum |
| title | Development and validation of LC/APCI-MS method for the quantification of oat ceramides in skin permeation studies |
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