Monitoring UV‐induced signalling pathways in an ex vivo skin organ culture model using phospho‐antibody array
We investigated UV‐induced signalling in an ex vivo skin organ culture model using phospho‐antibody array. Phosphorylation modulations were analysed in time‐course experiments following exposure to solar‐simulated UV and validated by Western blot analyses. We found that UV induced P‐p38 and its subs...
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| Veröffentlicht in: | Experimental dermatology 2018-05, Vol.27 (5), p.470-472 |
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| container_title | Experimental dermatology |
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| creator | Lenain, Christelle Gamboa, Bastien Perrin, Agnes Séraïdaris, Alexia Bertino, Béatrice Rival, Yves Bernardi, Mathieu Piwnica, David Méhul, Bruno |
| description | We investigated UV‐induced signalling in an ex vivo skin organ culture model using phospho‐antibody array. Phosphorylation modulations were analysed in time‐course experiments following exposure to solar‐simulated UV and validated by Western blot analyses. We found that UV induced P‐p38 and its substrates, P‐ERK1/2 and P‐AKT, which were previously shown to be upregulated by UV in cultured keratinocytes and in vivo human skin. This indicates that phospho‐antibody array applied to ex vivo skin organ culture is a relevant experimental system to investigate signalling events following perturbations. As the identified proteins are components of pathways implicated in skin tumorigenesis, UV‐exposed skin organ culture model could be used to investigate the effect on these pathways of NMSC cancer drug candidates. In addition, we found that phospho‐HCK is induced upon UV exposure, producing a new candidate for future studies investigating its role in the skin response to UV and UV‐induced carcinogenesis. |
| doi_str_mv | 10.1111/exd.13440 |
| format | Article |
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Phosphorylation modulations were analysed in time‐course experiments following exposure to solar‐simulated UV and validated by Western blot analyses. We found that UV induced P‐p38 and its substrates, P‐ERK1/2 and P‐AKT, which were previously shown to be upregulated by UV in cultured keratinocytes and in vivo human skin. This indicates that phospho‐antibody array applied to ex vivo skin organ culture is a relevant experimental system to investigate signalling events following perturbations. As the identified proteins are components of pathways implicated in skin tumorigenesis, UV‐exposed skin organ culture model could be used to investigate the effect on these pathways of NMSC cancer drug candidates. In addition, we found that phospho‐HCK is induced upon UV exposure, producing a new candidate for future studies investigating its role in the skin response to UV and UV‐induced carcinogenesis.</description><identifier>ISSN: 0906-6705</identifier><identifier>EISSN: 1600-0625</identifier><identifier>DOI: 10.1111/exd.13440</identifier><identifier>PMID: 28887818</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Adult ; Aged ; AKT protein ; Antibodies, Phospho-Specific - analysis ; antibody phospho‐array ; Cancer ; Carcinogenesis ; Drug development ; ex vivo skin ; Female ; Humans ; Keratinocytes ; Male ; Middle Aged ; Models, Biological ; Organ culture ; Organ Culture Techniques ; Phosphorylation ; Protein Array Analysis ; Signal transduction ; Signal Transduction - radiation effects ; signalling ; Skin ; Skin - radiation effects ; skin tumorigenesis ; Tumorigenesis ; Ultraviolet radiation ; Ultraviolet Rays</subject><ispartof>Experimental dermatology, 2018-05, Vol.27 (5), p.470-472</ispartof><rights>2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>2018 John Wiley & Sons A/S. 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Phosphorylation modulations were analysed in time‐course experiments following exposure to solar‐simulated UV and validated by Western blot analyses. We found that UV induced P‐p38 and its substrates, P‐ERK1/2 and P‐AKT, which were previously shown to be upregulated by UV in cultured keratinocytes and in vivo human skin. This indicates that phospho‐antibody array applied to ex vivo skin organ culture is a relevant experimental system to investigate signalling events following perturbations. As the identified proteins are components of pathways implicated in skin tumorigenesis, UV‐exposed skin organ culture model could be used to investigate the effect on these pathways of NMSC cancer drug candidates. In addition, we found that phospho‐HCK is induced upon UV exposure, producing a new candidate for future studies investigating its role in the skin response to UV and UV‐induced carcinogenesis.</description><subject>Adult</subject><subject>Aged</subject><subject>AKT protein</subject><subject>Antibodies, Phospho-Specific - analysis</subject><subject>antibody phospho‐array</subject><subject>Cancer</subject><subject>Carcinogenesis</subject><subject>Drug development</subject><subject>ex vivo skin</subject><subject>Female</subject><subject>Humans</subject><subject>Keratinocytes</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Models, Biological</subject><subject>Organ culture</subject><subject>Organ Culture Techniques</subject><subject>Phosphorylation</subject><subject>Protein Array Analysis</subject><subject>Signal transduction</subject><subject>Signal Transduction - radiation effects</subject><subject>signalling</subject><subject>Skin</subject><subject>Skin - radiation effects</subject><subject>skin tumorigenesis</subject><subject>Tumorigenesis</subject><subject>Ultraviolet radiation</subject><subject>Ultraviolet Rays</subject><issn>0906-6705</issn><issn>1600-0625</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kN1OwyAYhonR6Pw58AYMiUcedINSCj00On-SGU_UeNZAoRuzKxPabT3zErxGr0S06plfQr7w5uFJeAE4xmiIw4z0Rg0xSRK0BQY4RShCaUy3wQBlKI1Shuge2Pd-jhBmhNFdsBdzzhnHfABe72xtGutMPYWPTx9v76ZWbaEV9GZai6r6ypeima1F56Gpoaih3sCVWVnoX8LdummIirZqWqfhwipdwdZ_v5pZH05Qirox0qoOCudEdwh2SlF5ffSzD8Dj1fjh4iaa3F_fXpxPooJwjiKqNSEiyViMEkkSSQVLMZdSaspUIXAieSl0QmMdZ5mknCmW0pSyLCspTlRGDsBp7106-9pq3-Rz27rwJ5_HiOKUkpiTQJ31VOGs906X-dKZhXBdjlH-VW4eys2_yw3syY-xlQut_sjfNgMw6oG1qXT3vykfP1_2yk_7LIZr</recordid><startdate>201805</startdate><enddate>201805</enddate><creator>Lenain, Christelle</creator><creator>Gamboa, Bastien</creator><creator>Perrin, Agnes</creator><creator>Séraïdaris, Alexia</creator><creator>Bertino, Béatrice</creator><creator>Rival, Yves</creator><creator>Bernardi, Mathieu</creator><creator>Piwnica, David</creator><creator>Méhul, Bruno</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><orcidid>https://orcid.org/0000-0002-3521-6413</orcidid></search><sort><creationdate>201805</creationdate><title>Monitoring UV‐induced signalling pathways in an ex vivo skin organ culture model using phospho‐antibody array</title><author>Lenain, Christelle ; Gamboa, Bastien ; Perrin, Agnes ; Séraïdaris, Alexia ; Bertino, Béatrice ; Rival, Yves ; Bernardi, Mathieu ; Piwnica, David ; Méhul, Bruno</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3880-5ee33a497204b34b5a7618bbbe57dca14b8fae452e299b587d76565799f514d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adult</topic><topic>Aged</topic><topic>AKT protein</topic><topic>Antibodies, Phospho-Specific - analysis</topic><topic>antibody phospho‐array</topic><topic>Cancer</topic><topic>Carcinogenesis</topic><topic>Drug development</topic><topic>ex vivo skin</topic><topic>Female</topic><topic>Humans</topic><topic>Keratinocytes</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Models, Biological</topic><topic>Organ culture</topic><topic>Organ Culture Techniques</topic><topic>Phosphorylation</topic><topic>Protein Array Analysis</topic><topic>Signal transduction</topic><topic>Signal Transduction - radiation effects</topic><topic>signalling</topic><topic>Skin</topic><topic>Skin - radiation effects</topic><topic>skin tumorigenesis</topic><topic>Tumorigenesis</topic><topic>Ultraviolet radiation</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lenain, Christelle</creatorcontrib><creatorcontrib>Gamboa, Bastien</creatorcontrib><creatorcontrib>Perrin, Agnes</creatorcontrib><creatorcontrib>Séraïdaris, Alexia</creatorcontrib><creatorcontrib>Bertino, Béatrice</creatorcontrib><creatorcontrib>Rival, Yves</creatorcontrib><creatorcontrib>Bernardi, Mathieu</creatorcontrib><creatorcontrib>Piwnica, David</creatorcontrib><creatorcontrib>Méhul, Bruno</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Experimental dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lenain, Christelle</au><au>Gamboa, Bastien</au><au>Perrin, Agnes</au><au>Séraïdaris, Alexia</au><au>Bertino, Béatrice</au><au>Rival, Yves</au><au>Bernardi, Mathieu</au><au>Piwnica, David</au><au>Méhul, Bruno</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monitoring UV‐induced signalling pathways in an ex vivo skin organ culture model using phospho‐antibody array</atitle><jtitle>Experimental dermatology</jtitle><addtitle>Exp Dermatol</addtitle><date>2018-05</date><risdate>2018</risdate><volume>27</volume><issue>5</issue><spage>470</spage><epage>472</epage><pages>470-472</pages><issn>0906-6705</issn><eissn>1600-0625</eissn><abstract>We investigated UV‐induced signalling in an ex vivo skin organ culture model using phospho‐antibody array. Phosphorylation modulations were analysed in time‐course experiments following exposure to solar‐simulated UV and validated by Western blot analyses. We found that UV induced P‐p38 and its substrates, P‐ERK1/2 and P‐AKT, which were previously shown to be upregulated by UV in cultured keratinocytes and in vivo human skin. This indicates that phospho‐antibody array applied to ex vivo skin organ culture is a relevant experimental system to investigate signalling events following perturbations. As the identified proteins are components of pathways implicated in skin tumorigenesis, UV‐exposed skin organ culture model could be used to investigate the effect on these pathways of NMSC cancer drug candidates. 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| subjects | Adult Aged AKT protein Antibodies, Phospho-Specific - analysis antibody phospho‐array Cancer Carcinogenesis Drug development ex vivo skin Female Humans Keratinocytes Male Middle Aged Models, Biological Organ culture Organ Culture Techniques Phosphorylation Protein Array Analysis Signal transduction Signal Transduction - radiation effects signalling Skin Skin - radiation effects skin tumorigenesis Tumorigenesis Ultraviolet radiation Ultraviolet Rays |
| title | Monitoring UV‐induced signalling pathways in an ex vivo skin organ culture model using phospho‐antibody array |
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