Immunostimulatory effect of pepsin enzymatic extract from Porphyra yezoensis on murine splenocytes
BACKGROUND Porphyra yezoensis is a red alga consumed mainly in Korea, Japan, and China for food. This study researches the immunological effect of pepsin extract of P. yezoensis (PPEE) on murine splenocytes. RESULTS PPEE was not toxic on murine splenocytes and dramatically increased the proliferatio...
Gespeichert in:
| Veröffentlicht in: | Journal of the science of food and agriculture 2018-07, Vol.98 (9), p.3400-3408 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3408 |
|---|---|
| container_issue | 9 |
| container_start_page | 3400 |
| container_title | Journal of the science of food and agriculture |
| container_volume | 98 |
| creator | Herath, Khin Madushani Lee, Jeong Ha Cho, Jinhee Kim, Areum Shin, Su Min Kim, Bohyung Jeon, You‐Jin Jee, Youngheun |
| description | BACKGROUND
Porphyra yezoensis is a red alga consumed mainly in Korea, Japan, and China for food. This study researches the immunological effect of pepsin extract of P. yezoensis (PPEE) on murine splenocytes.
RESULTS
PPEE was not toxic on murine splenocytes and dramatically increased the proliferation of splenocytes compared with untreated control. Flow cytometry assay performed to sum up the effect of PPEE (31.3 and 62.5 µg mL−1) on major immune cells revealed that PPEE had no effect on the function of CD3e+CD4+ T‐helper cells, CD3e+CD8+ T‐cytotoxic cells, or CD44+CD62L‐ effector T cells in splenocytes compared with untreated control. More importantly, CD45+CD11b+ macrophage and dendritic cell populations and Ly‐6C+Ly‐6G+ macrophages/monocytes in splenocytes were activated by PPEE treatment compared with untreated control. Further experiments showed that PPEE treatment increased the secretion of macrophage‐derived cytokines such as interleukin‐1β, tumor necrosis factor‐α, and interleukin‐12, and macrophage‐activating cytokines interferon‐γ and interleukin‐10 compared with untreated control.
CONCLUSION
Taken together, these results suggest that PPEE has an immune stimulatory effect on macrophages, dendritic cells, and memory T cells. This property signifies the potential medicinal value of PPEE in clinical implications for immune‐compromised diseases. © 2017 Society of Chemical Industry |
| doi_str_mv | 10.1002/jsfa.8851 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2050516739</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2050516739</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3531-3f357c79fcf76831579a336a480e7b71dc7fc2d532b005beec2b2f24495f94d73</originalsourceid><addsrcrecordid>eNp1kEFLxDAQRoMouq4e_AMS8OShOkmapjmKuLoiKKjnkmYn2GXb1KRFu7_erqvePM3he7yBR8gJgwsGwC-X0ZmLPJdsh0wYaJUAMNglk3HjiWQpPyCHMS4BQOss2ycHXPMcWCompJzXdd_42FV1vzKdDwNF59B21DvaYhurhmKzHmrTVZbiZxfMuLnga_rkQ_s2BEMHXHtsYhWpb2jdh6pBGtsVNt4OHcYjsufMKuLxz52S19nNy_Vd8vB4O7--ekiskIIlwgmprNLOOpXlgkmljRCZSXNAVSq2sMpZvpCClwCyRLS85I6nqZZOpwslpuRs622Df-8xdsXS96EZXxYcJEiWKaFH6nxL2eBjDOiKNlS1CUPBoNjULDY1i03NkT39MfZljYs_8jffCFxugY9qhcP_puL-eXb1rfwCUTKAwA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2050516739</pqid></control><display><type>article</type><title>Immunostimulatory effect of pepsin enzymatic extract from Porphyra yezoensis on murine splenocytes</title><source>Wiley Online Library Journals Frontfile Complete</source><creator>Herath, Khin Madushani ; Lee, Jeong Ha ; Cho, Jinhee ; Kim, Areum ; Shin, Su Min ; Kim, Bohyung ; Jeon, You‐Jin ; Jee, Youngheun</creator><creatorcontrib>Herath, Khin Madushani ; Lee, Jeong Ha ; Cho, Jinhee ; Kim, Areum ; Shin, Su Min ; Kim, Bohyung ; Jeon, You‐Jin ; Jee, Youngheun</creatorcontrib><description>BACKGROUND
Porphyra yezoensis is a red alga consumed mainly in Korea, Japan, and China for food. This study researches the immunological effect of pepsin extract of P. yezoensis (PPEE) on murine splenocytes.
RESULTS
PPEE was not toxic on murine splenocytes and dramatically increased the proliferation of splenocytes compared with untreated control. Flow cytometry assay performed to sum up the effect of PPEE (31.3 and 62.5 µg mL−1) on major immune cells revealed that PPEE had no effect on the function of CD3e+CD4+ T‐helper cells, CD3e+CD8+ T‐cytotoxic cells, or CD44+CD62L‐ effector T cells in splenocytes compared with untreated control. More importantly, CD45+CD11b+ macrophage and dendritic cell populations and Ly‐6C+Ly‐6G+ macrophages/monocytes in splenocytes were activated by PPEE treatment compared with untreated control. Further experiments showed that PPEE treatment increased the secretion of macrophage‐derived cytokines such as interleukin‐1β, tumor necrosis factor‐α, and interleukin‐12, and macrophage‐activating cytokines interferon‐γ and interleukin‐10 compared with untreated control.
CONCLUSION
Taken together, these results suggest that PPEE has an immune stimulatory effect on macrophages, dendritic cells, and memory T cells. This property signifies the potential medicinal value of PPEE in clinical implications for immune‐compromised diseases. © 2017 Society of Chemical Industry</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.8851</identifier><identifier>PMID: 29280143</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>CD11b antigen ; CD4 antigen ; CD44 antigen ; CD45 antigen ; CD8 antigen ; Cytokines ; Cytotoxicity ; Dendritic cells ; Effector cells ; Flow cytometry ; Helper cells ; Immune system ; Immunological memory ; Immunology ; Immunostimulation ; Interferon ; Interleukins ; L-selectin ; Lymphocytes ; Lymphocytes T ; Macrophages ; macrophage‐activating cytokines ; macrophage‐derived cytokines ; Memory cells ; Monocytes ; Organic chemistry ; Pepsin ; Porphyra ; Porphyra yezoensis ; Splenocytes</subject><ispartof>Journal of the science of food and agriculture, 2018-07, Vol.98 (9), p.3400-3408</ispartof><rights>2017 Society of Chemical Industry</rights><rights>This article is protected by copyright. All rights reserved.</rights><rights>2018 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3531-3f357c79fcf76831579a336a480e7b71dc7fc2d532b005beec2b2f24495f94d73</citedby><cites>FETCH-LOGICAL-c3531-3f357c79fcf76831579a336a480e7b71dc7fc2d532b005beec2b2f24495f94d73</cites><orcidid>0000-0002-6295-0314</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjsfa.8851$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjsfa.8851$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29280143$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Herath, Khin Madushani</creatorcontrib><creatorcontrib>Lee, Jeong Ha</creatorcontrib><creatorcontrib>Cho, Jinhee</creatorcontrib><creatorcontrib>Kim, Areum</creatorcontrib><creatorcontrib>Shin, Su Min</creatorcontrib><creatorcontrib>Kim, Bohyung</creatorcontrib><creatorcontrib>Jeon, You‐Jin</creatorcontrib><creatorcontrib>Jee, Youngheun</creatorcontrib><title>Immunostimulatory effect of pepsin enzymatic extract from Porphyra yezoensis on murine splenocytes</title><title>Journal of the science of food and agriculture</title><addtitle>J Sci Food Agric</addtitle><description>BACKGROUND
Porphyra yezoensis is a red alga consumed mainly in Korea, Japan, and China for food. This study researches the immunological effect of pepsin extract of P. yezoensis (PPEE) on murine splenocytes.
RESULTS
PPEE was not toxic on murine splenocytes and dramatically increased the proliferation of splenocytes compared with untreated control. Flow cytometry assay performed to sum up the effect of PPEE (31.3 and 62.5 µg mL−1) on major immune cells revealed that PPEE had no effect on the function of CD3e+CD4+ T‐helper cells, CD3e+CD8+ T‐cytotoxic cells, or CD44+CD62L‐ effector T cells in splenocytes compared with untreated control. More importantly, CD45+CD11b+ macrophage and dendritic cell populations and Ly‐6C+Ly‐6G+ macrophages/monocytes in splenocytes were activated by PPEE treatment compared with untreated control. Further experiments showed that PPEE treatment increased the secretion of macrophage‐derived cytokines such as interleukin‐1β, tumor necrosis factor‐α, and interleukin‐12, and macrophage‐activating cytokines interferon‐γ and interleukin‐10 compared with untreated control.
CONCLUSION
Taken together, these results suggest that PPEE has an immune stimulatory effect on macrophages, dendritic cells, and memory T cells. This property signifies the potential medicinal value of PPEE in clinical implications for immune‐compromised diseases. © 2017 Society of Chemical Industry</description><subject>CD11b antigen</subject><subject>CD4 antigen</subject><subject>CD44 antigen</subject><subject>CD45 antigen</subject><subject>CD8 antigen</subject><subject>Cytokines</subject><subject>Cytotoxicity</subject><subject>Dendritic cells</subject><subject>Effector cells</subject><subject>Flow cytometry</subject><subject>Helper cells</subject><subject>Immune system</subject><subject>Immunological memory</subject><subject>Immunology</subject><subject>Immunostimulation</subject><subject>Interferon</subject><subject>Interleukins</subject><subject>L-selectin</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Macrophages</subject><subject>macrophage‐activating cytokines</subject><subject>macrophage‐derived cytokines</subject><subject>Memory cells</subject><subject>Monocytes</subject><subject>Organic chemistry</subject><subject>Pepsin</subject><subject>Porphyra</subject><subject>Porphyra yezoensis</subject><subject>Splenocytes</subject><issn>0022-5142</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kEFLxDAQRoMouq4e_AMS8OShOkmapjmKuLoiKKjnkmYn2GXb1KRFu7_erqvePM3he7yBR8gJgwsGwC-X0ZmLPJdsh0wYaJUAMNglk3HjiWQpPyCHMS4BQOss2ycHXPMcWCompJzXdd_42FV1vzKdDwNF59B21DvaYhurhmKzHmrTVZbiZxfMuLnga_rkQ_s2BEMHXHtsYhWpb2jdh6pBGtsVNt4OHcYjsufMKuLxz52S19nNy_Vd8vB4O7--ekiskIIlwgmprNLOOpXlgkmljRCZSXNAVSq2sMpZvpCClwCyRLS85I6nqZZOpwslpuRs622Df-8xdsXS96EZXxYcJEiWKaFH6nxL2eBjDOiKNlS1CUPBoNjULDY1i03NkT39MfZljYs_8jffCFxugY9qhcP_puL-eXb1rfwCUTKAwA</recordid><startdate>201807</startdate><enddate>201807</enddate><creator>Herath, Khin Madushani</creator><creator>Lee, Jeong Ha</creator><creator>Cho, Jinhee</creator><creator>Kim, Areum</creator><creator>Shin, Su Min</creator><creator>Kim, Bohyung</creator><creator>Jeon, You‐Jin</creator><creator>Jee, Youngheun</creator><general>John Wiley & Sons, Ltd</general><general>John Wiley and Sons, Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QL</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7ST</scope><scope>7T5</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0002-6295-0314</orcidid></search><sort><creationdate>201807</creationdate><title>Immunostimulatory effect of pepsin enzymatic extract from Porphyra yezoensis on murine splenocytes</title><author>Herath, Khin Madushani ; Lee, Jeong Ha ; Cho, Jinhee ; Kim, Areum ; Shin, Su Min ; Kim, Bohyung ; Jeon, You‐Jin ; Jee, Youngheun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3531-3f357c79fcf76831579a336a480e7b71dc7fc2d532b005beec2b2f24495f94d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>CD11b antigen</topic><topic>CD4 antigen</topic><topic>CD44 antigen</topic><topic>CD45 antigen</topic><topic>CD8 antigen</topic><topic>Cytokines</topic><topic>Cytotoxicity</topic><topic>Dendritic cells</topic><topic>Effector cells</topic><topic>Flow cytometry</topic><topic>Helper cells</topic><topic>Immune system</topic><topic>Immunological memory</topic><topic>Immunology</topic><topic>Immunostimulation</topic><topic>Interferon</topic><topic>Interleukins</topic><topic>L-selectin</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Macrophages</topic><topic>macrophage‐activating cytokines</topic><topic>macrophage‐derived cytokines</topic><topic>Memory cells</topic><topic>Monocytes</topic><topic>Organic chemistry</topic><topic>Pepsin</topic><topic>Porphyra</topic><topic>Porphyra yezoensis</topic><topic>Splenocytes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Herath, Khin Madushani</creatorcontrib><creatorcontrib>Lee, Jeong Ha</creatorcontrib><creatorcontrib>Cho, Jinhee</creatorcontrib><creatorcontrib>Kim, Areum</creatorcontrib><creatorcontrib>Shin, Su Min</creatorcontrib><creatorcontrib>Kim, Bohyung</creatorcontrib><creatorcontrib>Jeon, You‐Jin</creatorcontrib><creatorcontrib>Jee, Youngheun</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Herath, Khin Madushani</au><au>Lee, Jeong Ha</au><au>Cho, Jinhee</au><au>Kim, Areum</au><au>Shin, Su Min</au><au>Kim, Bohyung</au><au>Jeon, You‐Jin</au><au>Jee, Youngheun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunostimulatory effect of pepsin enzymatic extract from Porphyra yezoensis on murine splenocytes</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J Sci Food Agric</addtitle><date>2018-07</date><risdate>2018</risdate><volume>98</volume><issue>9</issue><spage>3400</spage><epage>3408</epage><pages>3400-3408</pages><issn>0022-5142</issn><eissn>1097-0010</eissn><abstract>BACKGROUND
Porphyra yezoensis is a red alga consumed mainly in Korea, Japan, and China for food. This study researches the immunological effect of pepsin extract of P. yezoensis (PPEE) on murine splenocytes.
RESULTS
PPEE was not toxic on murine splenocytes and dramatically increased the proliferation of splenocytes compared with untreated control. Flow cytometry assay performed to sum up the effect of PPEE (31.3 and 62.5 µg mL−1) on major immune cells revealed that PPEE had no effect on the function of CD3e+CD4+ T‐helper cells, CD3e+CD8+ T‐cytotoxic cells, or CD44+CD62L‐ effector T cells in splenocytes compared with untreated control. More importantly, CD45+CD11b+ macrophage and dendritic cell populations and Ly‐6C+Ly‐6G+ macrophages/monocytes in splenocytes were activated by PPEE treatment compared with untreated control. Further experiments showed that PPEE treatment increased the secretion of macrophage‐derived cytokines such as interleukin‐1β, tumor necrosis factor‐α, and interleukin‐12, and macrophage‐activating cytokines interferon‐γ and interleukin‐10 compared with untreated control.
CONCLUSION
Taken together, these results suggest that PPEE has an immune stimulatory effect on macrophages, dendritic cells, and memory T cells. This property signifies the potential medicinal value of PPEE in clinical implications for immune‐compromised diseases. © 2017 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>29280143</pmid><doi>10.1002/jsfa.8851</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-6295-0314</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-5142 |
| ispartof | Journal of the science of food and agriculture, 2018-07, Vol.98 (9), p.3400-3408 |
| issn | 0022-5142 1097-0010 |
| language | eng |
| recordid | cdi_proquest_journals_2050516739 |
| source | Wiley Online Library Journals Frontfile Complete |
| subjects | CD11b antigen CD4 antigen CD44 antigen CD45 antigen CD8 antigen Cytokines Cytotoxicity Dendritic cells Effector cells Flow cytometry Helper cells Immune system Immunological memory Immunology Immunostimulation Interferon Interleukins L-selectin Lymphocytes Lymphocytes T Macrophages macrophage‐activating cytokines macrophage‐derived cytokines Memory cells Monocytes Organic chemistry Pepsin Porphyra Porphyra yezoensis Splenocytes |
| title | Immunostimulatory effect of pepsin enzymatic extract from Porphyra yezoensis on murine splenocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T14%3A25%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immunostimulatory%20effect%20of%20pepsin%20enzymatic%20extract%20from%20Porphyra%20yezoensis%20on%20murine%20splenocytes&rft.jtitle=Journal%20of%20the%20science%20of%20food%20and%20agriculture&rft.au=Herath,%20Khin%20Madushani&rft.date=2018-07&rft.volume=98&rft.issue=9&rft.spage=3400&rft.epage=3408&rft.pages=3400-3408&rft.issn=0022-5142&rft.eissn=1097-0010&rft_id=info:doi/10.1002/jsfa.8851&rft_dat=%3Cproquest_cross%3E2050516739%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2050516739&rft_id=info:pmid/29280143&rfr_iscdi=true |