How Does Experimental Design Modify the Result of Daphnia magna Heartbeat Rate Test? ─ Analyses of Factors Affecting the Sensitivity of the Test System
Development of an unconventional test method involves usually the comparison of biological responses under a variety of test conditions. The quality of these biological methods relies on an appropriate experimental design. The Daphnia magna heartbeat rate as a physiological endpoint for assessing aq...
Gespeichert in:
| Veröffentlicht in: | Periodica polytechnica. Chemical engineering. 2018-01, Vol.62 (3), p.257-264 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 264 |
|---|---|
| container_issue | 3 |
| container_start_page | 257 |
| container_title | Periodica polytechnica. Chemical engineering. |
| container_volume | 62 |
| creator | Fekete-Kertész, Ildikó Stirling, Tamás Ullmann, Orsolya Farkas, Éva Kirchkeszner, Csaba Feigl, Viktória Molnár, Mónika |
| description | Development of an unconventional test method involves usually the comparison of biological responses under a variety of test conditions. The quality of these biological methods relies on an appropriate experimental design. The Daphnia magna heartbeat rate as a physiological endpoint for assessing aquatic pollution has been of minor interest so far; nonetheless, this could be an early and sensitive indicator of the harmful effect of micropollutants. Our aim was to set up the optimal experimental design of the heartbeat rate test. The studied factors were the composition of the test medium, the age of the test organism, and the exposure time, at triclosan concentrations between 0.2–2000 μg/L. According to the evaluation of test results the optimal test condition for the heartbeat rate test assumes tap water as test medium, 10-day-old test organisms and 48 h exposure time. |
| doi_str_mv | 10.3311/PPch.10841 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2048838010</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2048838010</sourcerecordid><originalsourceid>FETCH-LOGICAL-c295t-ec3d6f2ed2989bffe52f1874cf4e05953de86dc4fe4dc43d687e29d0c438a1f73</originalsourceid><addsrcrecordid>eNotkE1OwzAQhS0EElXphhNYYoeU4p84cVao6g9FKqJqyzpyk3FrlCYldoHsOAQbrsdJcFo2M6PRe29GH0LXlPQ5p_RuPs-2fUpkSM9QhwoZBzyOxDnqEM7CQEjBL1HPWrMmJGJSRHHcQT_T6gOPKrB4_LmH2uygdKrAI7BmU-KnKje6wW4LeAH2UDhcaTxS-21pFN6pTanwFFTt1qAcXigHeAXW3ePf7y88KFXRWB_sLROVuaq2eKA1ZM6Um2PkEkprnHk3rmlF7aq142VjHeyu0IVWhYXef--il8l4NZwGs-eHx-FgFmQsES6AjOeRZpCzRCZrny-YpjIOMx0CEYngOcgoz0INoa9eK2NgSU78LBXVMe-im1Puvq7eDv5--lodav-8TRkJpeSSUOJVtydVVlfW1qDTvYel6ialJG3ppy399Eif_wGbFXpi</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2048838010</pqid></control><display><type>article</type><title>How Does Experimental Design Modify the Result of Daphnia magna Heartbeat Rate Test? ─ Analyses of Factors Affecting the Sensitivity of the Test System</title><source>DOAJ Directory of Open Access Journals</source><creator>Fekete-Kertész, Ildikó ; Stirling, Tamás ; Ullmann, Orsolya ; Farkas, Éva ; Kirchkeszner, Csaba ; Feigl, Viktória ; Molnár, Mónika</creator><creatorcontrib>Fekete-Kertész, Ildikó ; Stirling, Tamás ; Ullmann, Orsolya ; Farkas, Éva ; Kirchkeszner, Csaba ; Feigl, Viktória ; Molnár, Mónika</creatorcontrib><description>Development of an unconventional test method involves usually the comparison of biological responses under a variety of test conditions. The quality of these biological methods relies on an appropriate experimental design. The Daphnia magna heartbeat rate as a physiological endpoint for assessing aquatic pollution has been of minor interest so far; nonetheless, this could be an early and sensitive indicator of the harmful effect of micropollutants. Our aim was to set up the optimal experimental design of the heartbeat rate test. The studied factors were the composition of the test medium, the age of the test organism, and the exposure time, at triclosan concentrations between 0.2–2000 μg/L. According to the evaluation of test results the optimal test condition for the heartbeat rate test assumes tap water as test medium, 10-day-old test organisms and 48 h exposure time.</description><identifier>ISSN: 0324-5853</identifier><identifier>EISSN: 1587-3765</identifier><identifier>DOI: 10.3311/PPch.10841</identifier><language>eng</language><publisher>Budapest: Periodica Polytechnica, Budapest University of Technology and Economics</publisher><subject>Design modifications ; Drinking water ; Sensitivity analysis ; Test procedures ; Water pollution</subject><ispartof>Periodica polytechnica. Chemical engineering., 2018-01, Vol.62 (3), p.257-264</ispartof><rights>Copyright Periodica Polytechnica, Budapest University of Technology and Economics 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c295t-ec3d6f2ed2989bffe52f1874cf4e05953de86dc4fe4dc43d687e29d0c438a1f73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27903,27904</link.rule.ids></links><search><creatorcontrib>Fekete-Kertész, Ildikó</creatorcontrib><creatorcontrib>Stirling, Tamás</creatorcontrib><creatorcontrib>Ullmann, Orsolya</creatorcontrib><creatorcontrib>Farkas, Éva</creatorcontrib><creatorcontrib>Kirchkeszner, Csaba</creatorcontrib><creatorcontrib>Feigl, Viktória</creatorcontrib><creatorcontrib>Molnár, Mónika</creatorcontrib><title>How Does Experimental Design Modify the Result of Daphnia magna Heartbeat Rate Test? ─ Analyses of Factors Affecting the Sensitivity of the Test System</title><title>Periodica polytechnica. Chemical engineering.</title><description>Development of an unconventional test method involves usually the comparison of biological responses under a variety of test conditions. The quality of these biological methods relies on an appropriate experimental design. The Daphnia magna heartbeat rate as a physiological endpoint for assessing aquatic pollution has been of minor interest so far; nonetheless, this could be an early and sensitive indicator of the harmful effect of micropollutants. Our aim was to set up the optimal experimental design of the heartbeat rate test. The studied factors were the composition of the test medium, the age of the test organism, and the exposure time, at triclosan concentrations between 0.2–2000 μg/L. According to the evaluation of test results the optimal test condition for the heartbeat rate test assumes tap water as test medium, 10-day-old test organisms and 48 h exposure time.</description><subject>Design modifications</subject><subject>Drinking water</subject><subject>Sensitivity analysis</subject><subject>Test procedures</subject><subject>Water pollution</subject><issn>0324-5853</issn><issn>1587-3765</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNotkE1OwzAQhS0EElXphhNYYoeU4p84cVao6g9FKqJqyzpyk3FrlCYldoHsOAQbrsdJcFo2M6PRe29GH0LXlPQ5p_RuPs-2fUpkSM9QhwoZBzyOxDnqEM7CQEjBL1HPWrMmJGJSRHHcQT_T6gOPKrB4_LmH2uygdKrAI7BmU-KnKje6wW4LeAH2UDhcaTxS-21pFN6pTanwFFTt1qAcXigHeAXW3ePf7y88KFXRWB_sLROVuaq2eKA1ZM6Um2PkEkprnHk3rmlF7aq142VjHeyu0IVWhYXef--il8l4NZwGs-eHx-FgFmQsES6AjOeRZpCzRCZrny-YpjIOMx0CEYngOcgoz0INoa9eK2NgSU78LBXVMe-im1Puvq7eDv5--lodav-8TRkJpeSSUOJVtydVVlfW1qDTvYel6ialJG3ppy399Eif_wGbFXpi</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Fekete-Kertész, Ildikó</creator><creator>Stirling, Tamás</creator><creator>Ullmann, Orsolya</creator><creator>Farkas, Éva</creator><creator>Kirchkeszner, Csaba</creator><creator>Feigl, Viktória</creator><creator>Molnár, Mónika</creator><general>Periodica Polytechnica, Budapest University of Technology and Economics</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>L6V</scope><scope>L7M</scope><scope>M7S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope></search><sort><creationdate>20180101</creationdate><title>How Does Experimental Design Modify the Result of Daphnia magna Heartbeat Rate Test? ─ Analyses of Factors Affecting the Sensitivity of the Test System</title><author>Fekete-Kertész, Ildikó ; Stirling, Tamás ; Ullmann, Orsolya ; Farkas, Éva ; Kirchkeszner, Csaba ; Feigl, Viktória ; Molnár, Mónika</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c295t-ec3d6f2ed2989bffe52f1874cf4e05953de86dc4fe4dc43d687e29d0c438a1f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Design modifications</topic><topic>Drinking water</topic><topic>Sensitivity analysis</topic><topic>Test procedures</topic><topic>Water pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fekete-Kertész, Ildikó</creatorcontrib><creatorcontrib>Stirling, Tamás</creatorcontrib><creatorcontrib>Ullmann, Orsolya</creatorcontrib><creatorcontrib>Farkas, Éva</creatorcontrib><creatorcontrib>Kirchkeszner, Csaba</creatorcontrib><creatorcontrib>Feigl, Viktória</creatorcontrib><creatorcontrib>Molnár, Mónika</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>East Europe, Central Europe Database</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Engineering Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><jtitle>Periodica polytechnica. Chemical engineering.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fekete-Kertész, Ildikó</au><au>Stirling, Tamás</au><au>Ullmann, Orsolya</au><au>Farkas, Éva</au><au>Kirchkeszner, Csaba</au><au>Feigl, Viktória</au><au>Molnár, Mónika</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>How Does Experimental Design Modify the Result of Daphnia magna Heartbeat Rate Test? ─ Analyses of Factors Affecting the Sensitivity of the Test System</atitle><jtitle>Periodica polytechnica. Chemical engineering.</jtitle><date>2018-01-01</date><risdate>2018</risdate><volume>62</volume><issue>3</issue><spage>257</spage><epage>264</epage><pages>257-264</pages><issn>0324-5853</issn><eissn>1587-3765</eissn><abstract>Development of an unconventional test method involves usually the comparison of biological responses under a variety of test conditions. The quality of these biological methods relies on an appropriate experimental design. The Daphnia magna heartbeat rate as a physiological endpoint for assessing aquatic pollution has been of minor interest so far; nonetheless, this could be an early and sensitive indicator of the harmful effect of micropollutants. Our aim was to set up the optimal experimental design of the heartbeat rate test. The studied factors were the composition of the test medium, the age of the test organism, and the exposure time, at triclosan concentrations between 0.2–2000 μg/L. According to the evaluation of test results the optimal test condition for the heartbeat rate test assumes tap water as test medium, 10-day-old test organisms and 48 h exposure time.</abstract><cop>Budapest</cop><pub>Periodica Polytechnica, Budapest University of Technology and Economics</pub><doi>10.3311/PPch.10841</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0324-5853 |
| ispartof | Periodica polytechnica. Chemical engineering., 2018-01, Vol.62 (3), p.257-264 |
| issn | 0324-5853 1587-3765 |
| language | eng |
| recordid | cdi_proquest_journals_2048838010 |
| source | DOAJ Directory of Open Access Journals |
| subjects | Design modifications Drinking water Sensitivity analysis Test procedures Water pollution |
| title | How Does Experimental Design Modify the Result of Daphnia magna Heartbeat Rate Test? ─ Analyses of Factors Affecting the Sensitivity of the Test System |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T22%3A57%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=How%20Does%20Experimental%20Design%20Modify%20the%20Result%20of%20Daphnia%20magna%20Heartbeat%20Rate%20Test?%20%E2%94%80%20Analyses%20of%20Factors%20Affecting%20the%20Sensitivity%20of%20the%20Test%20System&rft.jtitle=Periodica%20polytechnica.%20Chemical%20engineering.&rft.au=Fekete-Kert%C3%A9sz,%20Ildik%C3%B3&rft.date=2018-01-01&rft.volume=62&rft.issue=3&rft.spage=257&rft.epage=264&rft.pages=257-264&rft.issn=0324-5853&rft.eissn=1587-3765&rft_id=info:doi/10.3311/PPch.10841&rft_dat=%3Cproquest_cross%3E2048838010%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2048838010&rft_id=info:pmid/&rfr_iscdi=true |