Toxicological effects of PBDEs on carassius aurats

In this study the effects ofpolybrominated diphenyl ether compounds （PBDEs）, BDE-47 and BDE-99 on carassius aurats were discussed. The reproductive capacity viability and proliferation percent decreased with increasing BDE-47and BDE-99 concentrations （p〈0.05）, presenting a dose-dependent manner. Physiological biomarkers such as cholesterol （CHL）, acetylcholinesterase （ACHE）, ethoxyresorufin-0-deethylase （EROD） and vitamin were studied to reveal the toxicological effects of BDE-47 and BDE-99 exposure. CHL levels generally decrease with increasing BDE-47 exposure. However, there were no significant differences between the samples and the control. As to BDE-99, the CHL level decreased as compared with the control level when the BDE-99 concentrations reached 20 tig/L （p〈0.05）. The degree of inhibition of the AChE increased with the increase of DE-47and BDE-99 concentrations. The EROD activity during 72 hours＇ exposure significantly increased to 20 μg/L for BDE-47 （p〈0.05）, however, EROD activity was not significantly different at different concentrations of BDE-99 （p〈0.05）. Carassius aurats hepatocytes reacted to the BDE-47 exposure with increasing EROD activities in the dose dependent manner. Although at lower concentrations, the induced effects of BDE-47 and BDE-99 were not significantly different, though BDE-47 showed strong induced effects on the EROD activity with increasing concentrations in carassius aurats hepatocytes. In contrast, BDE-99 did not influence EROD activity consistently. The VA level of carassius aurats was significantly different from that of the controls for BDE-99 （p〈0.001）, as far as the VA level was significantly lower of carassius aurats than the controls for BDE-47 （p〈0.001）. CHL, ACHE, EROD, and VA levels in carassius aurats were differently affected by BDE-47 and BDE-99 exposures, therefore, they would be good indicators for exposure.