A coumarin-based fluorescent probe for monitoring labile ferrous iron in living systems
Labile Fe 2+ has been considered to be a metabolically active and regulatory form of cellular iron. Monitoring the dynamic level of labile Fe 2+ in biological systems is vital for evaluating the iron related biological processes and diseases as well as dissecting the exact physiological and pathophy...
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| Veröffentlicht in: | Analyst (London) 2018-05, Vol.143 (11), p.2555-2562 |
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| creator | Long, Lingliang Wang, Ning Han, Yuanyuan Huang, Meiyu Yuan, Xiangqi Cao, Siyu Gong, Aihua Wang, Kun |
| description | Labile Fe
2+
has been considered to be a metabolically active and regulatory form of cellular iron. Monitoring the dynamic level of labile Fe
2+
in biological systems is vital for evaluating the iron related biological processes and diseases as well as dissecting the exact physiological and pathophysiological functions of the labile Fe
2+
. Herein, we rationally constructed a coumarin-based fluorescent probe for sensing labile Fe
2+
in living systems based on a novel Fe
2+
meditated cyclization reaction strategy. The probe showed a highly selective and sensitive response to Fe
2+
, and the detection limit was determined to be 45 nM. Significantly, the probe displayed fast response to Fe
2+
, with the sensing reaction completed in 2 min, which is beneficial for real time sensing. The application of the probe for sensing different concentrations of labile Fe
2+
in living cells has been conducted. In addition, the basal and endogenous levels of labile Fe
2+
in living systems were also successfully monitored.
A fluorescent probe for sensing labile Fe
2+
levels has been rationally constructed based on a novel Fe
2+
mediated cyclization reaction. |
| doi_str_mv | 10.1039/c8an00556g |
| format | Article |
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2+
has been considered to be a metabolically active and regulatory form of cellular iron. Monitoring the dynamic level of labile Fe
2+
in biological systems is vital for evaluating the iron related biological processes and diseases as well as dissecting the exact physiological and pathophysiological functions of the labile Fe
2+
. Herein, we rationally constructed a coumarin-based fluorescent probe for sensing labile Fe
2+
in living systems based on a novel Fe
2+
meditated cyclization reaction strategy. The probe showed a highly selective and sensitive response to Fe
2+
, and the detection limit was determined to be 45 nM. Significantly, the probe displayed fast response to Fe
2+
, with the sensing reaction completed in 2 min, which is beneficial for real time sensing. The application of the probe for sensing different concentrations of labile Fe
2+
in living cells has been conducted. In addition, the basal and endogenous levels of labile Fe
2+
in living systems were also successfully monitored.
A fluorescent probe for sensing labile Fe
2+
levels has been rationally constructed based on a novel Fe
2+
mediated cyclization reaction.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/c8an00556g</identifier><identifier>PMID: 29721571</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Biological activity ; Coumarin ; Coumarins - chemistry ; Detection ; Fluorescent Dyes - chemistry ; Fluorescent indicators ; Hep G2 Cells ; Humans ; Iron ; Iron - analysis ; Limit of Detection ; Monitoring ; Systems analysis</subject><ispartof>Analyst (London), 2018-05, Vol.143 (11), p.2555-2562</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-d1057b39f4ec2a7504ca3c6ca64f28e4ae2aa5e41e0e8e444d57a0b33c9213e23</citedby><cites>FETCH-LOGICAL-c337t-d1057b39f4ec2a7504ca3c6ca64f28e4ae2aa5e41e0e8e444d57a0b33c9213e23</cites><orcidid>0000-0002-3589-2075</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2818,2819,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29721571$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Long, Lingliang</creatorcontrib><creatorcontrib>Wang, Ning</creatorcontrib><creatorcontrib>Han, Yuanyuan</creatorcontrib><creatorcontrib>Huang, Meiyu</creatorcontrib><creatorcontrib>Yuan, Xiangqi</creatorcontrib><creatorcontrib>Cao, Siyu</creatorcontrib><creatorcontrib>Gong, Aihua</creatorcontrib><creatorcontrib>Wang, Kun</creatorcontrib><title>A coumarin-based fluorescent probe for monitoring labile ferrous iron in living systems</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>Labile Fe
2+
has been considered to be a metabolically active and regulatory form of cellular iron. Monitoring the dynamic level of labile Fe
2+
in biological systems is vital for evaluating the iron related biological processes and diseases as well as dissecting the exact physiological and pathophysiological functions of the labile Fe
2+
. Herein, we rationally constructed a coumarin-based fluorescent probe for sensing labile Fe
2+
in living systems based on a novel Fe
2+
meditated cyclization reaction strategy. The probe showed a highly selective and sensitive response to Fe
2+
, and the detection limit was determined to be 45 nM. Significantly, the probe displayed fast response to Fe
2+
, with the sensing reaction completed in 2 min, which is beneficial for real time sensing. The application of the probe for sensing different concentrations of labile Fe
2+
in living cells has been conducted. In addition, the basal and endogenous levels of labile Fe
2+
in living systems were also successfully monitored.
A fluorescent probe for sensing labile Fe
2+
levels has been rationally constructed based on a novel Fe
2+
mediated cyclization reaction.</description><subject>Biological activity</subject><subject>Coumarin</subject><subject>Coumarins - chemistry</subject><subject>Detection</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent indicators</subject><subject>Hep G2 Cells</subject><subject>Humans</subject><subject>Iron</subject><subject>Iron - analysis</subject><subject>Limit of Detection</subject><subject>Monitoring</subject><subject>Systems analysis</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1LAzEQhoMotlYv3pWAFxFW87kfx1K0CkUvisclm50tKbtJTXaF_ntTWxU8DTPzMDx5g9A5JbeU8OJO58oSImW6PEBjylORSMnyQzQmhPCEpVKM0EkIq9hSIskxGrEiY1RmdIzep1i7oVPe2KRSAWrctIPzEDTYHq-9qwA3zuPOWdO7SC1xqyrTxil474aAjXcWG4tb87ndhk3ooQun6KhRbYCzfZ2gt4f719ljsniZP82mi0RznvVJHYWyiheNAM1UJonQiutUq1Q0LAehgCklQVAgEFshapkpUnGuC0Y5MD5B17u7UfVjgNCXnYnubassRLuSES5YEV_NI3r1D125wdtoFykhizxN8zRSNztKexeCh6ZcexPz2ZSUlNu4y1k-ff6Oex7hy_3Joeqg_kV_8o3AxQ7wQf9u__6LfwGFVYRf</recordid><startdate>20180529</startdate><enddate>20180529</enddate><creator>Long, Lingliang</creator><creator>Wang, Ning</creator><creator>Han, Yuanyuan</creator><creator>Huang, Meiyu</creator><creator>Yuan, Xiangqi</creator><creator>Cao, Siyu</creator><creator>Gong, Aihua</creator><creator>Wang, Kun</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3589-2075</orcidid></search><sort><creationdate>20180529</creationdate><title>A coumarin-based fluorescent probe for monitoring labile ferrous iron in living systems</title><author>Long, Lingliang ; Wang, Ning ; Han, Yuanyuan ; Huang, Meiyu ; Yuan, Xiangqi ; Cao, Siyu ; Gong, Aihua ; Wang, Kun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-d1057b39f4ec2a7504ca3c6ca64f28e4ae2aa5e41e0e8e444d57a0b33c9213e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Biological activity</topic><topic>Coumarin</topic><topic>Coumarins - chemistry</topic><topic>Detection</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorescent indicators</topic><topic>Hep G2 Cells</topic><topic>Humans</topic><topic>Iron</topic><topic>Iron - analysis</topic><topic>Limit of Detection</topic><topic>Monitoring</topic><topic>Systems analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Long, Lingliang</creatorcontrib><creatorcontrib>Wang, Ning</creatorcontrib><creatorcontrib>Han, Yuanyuan</creatorcontrib><creatorcontrib>Huang, Meiyu</creatorcontrib><creatorcontrib>Yuan, Xiangqi</creatorcontrib><creatorcontrib>Cao, Siyu</creatorcontrib><creatorcontrib>Gong, Aihua</creatorcontrib><creatorcontrib>Wang, Kun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Long, Lingliang</au><au>Wang, Ning</au><au>Han, Yuanyuan</au><au>Huang, Meiyu</au><au>Yuan, Xiangqi</au><au>Cao, Siyu</au><au>Gong, Aihua</au><au>Wang, Kun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A coumarin-based fluorescent probe for monitoring labile ferrous iron in living systems</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2018-05-29</date><risdate>2018</risdate><volume>143</volume><issue>11</issue><spage>2555</spage><epage>2562</epage><pages>2555-2562</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>Labile Fe
2+
has been considered to be a metabolically active and regulatory form of cellular iron. Monitoring the dynamic level of labile Fe
2+
in biological systems is vital for evaluating the iron related biological processes and diseases as well as dissecting the exact physiological and pathophysiological functions of the labile Fe
2+
. Herein, we rationally constructed a coumarin-based fluorescent probe for sensing labile Fe
2+
in living systems based on a novel Fe
2+
meditated cyclization reaction strategy. The probe showed a highly selective and sensitive response to Fe
2+
, and the detection limit was determined to be 45 nM. Significantly, the probe displayed fast response to Fe
2+
, with the sensing reaction completed in 2 min, which is beneficial for real time sensing. The application of the probe for sensing different concentrations of labile Fe
2+
in living cells has been conducted. In addition, the basal and endogenous levels of labile Fe
2+
in living systems were also successfully monitored.
A fluorescent probe for sensing labile Fe
2+
levels has been rationally constructed based on a novel Fe
2+
mediated cyclization reaction.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>29721571</pmid><doi>10.1039/c8an00556g</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-3589-2075</orcidid></addata></record> |
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| ispartof | Analyst (London), 2018-05, Vol.143 (11), p.2555-2562 |
| issn | 0003-2654 1364-5528 |
| language | eng |
| recordid | cdi_proquest_journals_2045986686 |
| source | Royal Society of Chemistry Journals Archive (1841-2007); MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Biological activity Coumarin Coumarins - chemistry Detection Fluorescent Dyes - chemistry Fluorescent indicators Hep G2 Cells Humans Iron Iron - analysis Limit of Detection Monitoring Systems analysis |
| title | A coumarin-based fluorescent probe for monitoring labile ferrous iron in living systems |
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