Molecular Detection of Northern Leatherside Chub (Lepidomeda copei) DNA in Environmental Samples
The northern leatherside chub (Lepidomeda copei) is a cyprinid fish native to the Snake River, Green River, and Bonneville basins of the western United States. Population declines prompted the development of a multistate conservation agreement and strategy, which emphasized the need to reliably deli...
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| Veröffentlicht in: | Western North American naturalist 2018-04, Vol.78 (1), p.92-99 |
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| creator | Dysthe, Joseph C. Carim, Kellie J. Franklin, Thomas W. Kikkert, Dave Young, Michael K. McKelvey, Kevin S. Schwartz, Michael K. |
| description | The northern leatherside chub (Lepidomeda copei) is a cyprinid fish native to the Snake River, Green River, and Bonneville basins of the western United States. Population declines prompted the development of a multistate conservation agreement and strategy, which emphasized the need to reliably delineate its current distribution and monitor its status. To facilitate species monitoring, we developed a quantitative PCR assay to detect northern leatherside chub DNA in environmental samples. The assay consistently detected northern leatherside chub DNA in concentrations as low as 2 copies per reaction and did not amplify DNA of potentially sympatric fish species. The assay amplified a synthetic DNA template representing 3 congeneric species: White River spinedace (L.albivallis), Virgin spinedace, (L.mollispinis mollispinis), and Big Spring spinedace, (L.m. pratensis); however, none of these are sympatric with northern leatherside chub. Field tests of the assay accurately reproduced expected patterns of species occupancy. |
| doi_str_mv | 10.3398/064.078.0109 |
| format | Article |
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Population declines prompted the development of a multistate conservation agreement and strategy, which emphasized the need to reliably delineate its current distribution and monitor its status. To facilitate species monitoring, we developed a quantitative PCR assay to detect northern leatherside chub DNA in environmental samples. The assay consistently detected northern leatherside chub DNA in concentrations as low as 2 copies per reaction and did not amplify DNA of potentially sympatric fish species. The assay amplified a synthetic DNA template representing 3 congeneric species: White River spinedace (L.albivallis), Virgin spinedace, (L.mollispinis mollispinis), and Big Spring spinedace, (L.m. pratensis); however, none of these are sympatric with northern leatherside chub. Field tests of the assay accurately reproduced expected patterns of species occupancy.</description><identifier>ISSN: 1527-0904</identifier><identifier>EISSN: 1944-8341</identifier><identifier>DOI: 10.3398/064.078.0109</identifier><language>eng</language><publisher>Provo: Brigham Young University</publisher><subject>Amplification ; Assaying ; Basins ; Biology ; Creeks & streams ; Current distribution ; Cyprinidae ; Cytochrome ; Deoxyribonucleic acid ; DNA ; DNA sequencing ; Ecology ; Endangered & extinct species ; Field tests ; Fish ; Genetic aspects ; Identification and classification ; Lepidomeda albivallis ; Lepidomeda copei ; Lepidomeda mollispinis mollispinis ; Lepidomeda mollispinis pratensis ; Methods ; Molecular chains ; Nucleotide sequencing ; Polymerase chain reaction ; Population decline ; Reproduction (biology) ; Rivers ; Sympatric populations ; Taxonomy ; Wildlife conservation</subject><ispartof>Western North American naturalist, 2018-04, Vol.78 (1), p.92-99</ispartof><rights>2018</rights><rights>COPYRIGHT 2018 Western North American Naturalist</rights><rights>Copyright Western North American Naturalist Apr 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b393t-d74c385e58ac05f393add80acc238327bbc96fce16995c9b594af4ae739e308b3</citedby><orcidid>0000-0002-0191-6112 ; 0000-0002-9622-9146 ; 0000-0002-6790-7841</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26779969$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26779969$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids></links><search><creatorcontrib>Dysthe, Joseph C.</creatorcontrib><creatorcontrib>Carim, Kellie J.</creatorcontrib><creatorcontrib>Franklin, Thomas W.</creatorcontrib><creatorcontrib>Kikkert, Dave</creatorcontrib><creatorcontrib>Young, Michael K.</creatorcontrib><creatorcontrib>McKelvey, Kevin S.</creatorcontrib><creatorcontrib>Schwartz, Michael K.</creatorcontrib><title>Molecular Detection of Northern Leatherside Chub (Lepidomeda copei) DNA in Environmental Samples</title><title>Western North American naturalist</title><description>The northern leatherside chub (Lepidomeda copei) is a cyprinid fish native to the Snake River, Green River, and Bonneville basins of the western United States. Population declines prompted the development of a multistate conservation agreement and strategy, which emphasized the need to reliably delineate its current distribution and monitor its status. To facilitate species monitoring, we developed a quantitative PCR assay to detect northern leatherside chub DNA in environmental samples. The assay consistently detected northern leatherside chub DNA in concentrations as low as 2 copies per reaction and did not amplify DNA of potentially sympatric fish species. The assay amplified a synthetic DNA template representing 3 congeneric species: White River spinedace (L.albivallis), Virgin spinedace, (L.mollispinis mollispinis), and Big Spring spinedace, (L.m. pratensis); however, none of these are sympatric with northern leatherside chub. Field tests of the assay accurately reproduced expected patterns of species occupancy.</description><subject>Amplification</subject><subject>Assaying</subject><subject>Basins</subject><subject>Biology</subject><subject>Creeks & streams</subject><subject>Current distribution</subject><subject>Cyprinidae</subject><subject>Cytochrome</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA sequencing</subject><subject>Ecology</subject><subject>Endangered & extinct species</subject><subject>Field tests</subject><subject>Fish</subject><subject>Genetic aspects</subject><subject>Identification and classification</subject><subject>Lepidomeda albivallis</subject><subject>Lepidomeda copei</subject><subject>Lepidomeda mollispinis mollispinis</subject><subject>Lepidomeda mollispinis pratensis</subject><subject>Methods</subject><subject>Molecular chains</subject><subject>Nucleotide sequencing</subject><subject>Polymerase chain reaction</subject><subject>Population decline</subject><subject>Reproduction (biology)</subject><subject>Rivers</subject><subject>Sympatric populations</subject><subject>Taxonomy</subject><subject>Wildlife conservation</subject><issn>1527-0904</issn><issn>1944-8341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kU1v1DAQhi0EEmXhxhXJEheKyOLE38fVtkClpUgUzsZxJq1XSZzaDoJ_j1dBSL2gOcxo5pkPzYvQy5psKdXqPRFsS6TakproR-is1oxVirL6cYl5IyuiCXuKnqV0JIQLytQZ-vE5DOCWwUZ8ARlc9mHCocfXIeY7iBM-gD0FyXeA93dLi98cYPZdGKGz2IUZ_Dm-uN5hP-HL6aePYRphynbAN3acB0jP0ZPeDgle_PUb9P3D5bf9p-rw5ePVfneoWqpprjrJHFUcuLKO8L7kbNcpYp1rqKKNbFunRe-gFlpzp1uume2ZBUk1UKJaukGv17lzDPcLpGyOYYlTWWkawqjgTDBeqO1K3doBjJ_6kKN1xToYvQsT9L7kd1w0DZWy_GiDzh80FCbDr3xrl5TM1c3Xh-y7lXUxpBShN3P0o42_TU3MSSBTBDJFIHMSqOCvVvyYcoj_2EZIqbU41d-u9daHctn_h_0BspCXsg</recordid><startdate>20180401</startdate><enddate>20180401</enddate><creator>Dysthe, Joseph C.</creator><creator>Carim, Kellie J.</creator><creator>Franklin, Thomas W.</creator><creator>Kikkert, Dave</creator><creator>Young, Michael K.</creator><creator>McKelvey, Kevin S.</creator><creator>Schwartz, Michael K.</creator><general>Brigham Young University</general><general>Monte L. 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Detection of Northern Leatherside Chub (Lepidomeda copei) DNA in Environmental Samples</title><author>Dysthe, Joseph C. ; Carim, Kellie J. ; Franklin, Thomas W. ; Kikkert, Dave ; Young, Michael K. ; McKelvey, Kevin S. ; Schwartz, Michael K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b393t-d74c385e58ac05f393add80acc238327bbc96fce16995c9b594af4ae739e308b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Amplification</topic><topic>Assaying</topic><topic>Basins</topic><topic>Biology</topic><topic>Creeks & streams</topic><topic>Current distribution</topic><topic>Cyprinidae</topic><topic>Cytochrome</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA sequencing</topic><topic>Ecology</topic><topic>Endangered & extinct species</topic><topic>Field tests</topic><topic>Fish</topic><topic>Genetic aspects</topic><topic>Identification and classification</topic><topic>Lepidomeda albivallis</topic><topic>Lepidomeda copei</topic><topic>Lepidomeda mollispinis mollispinis</topic><topic>Lepidomeda mollispinis pratensis</topic><topic>Methods</topic><topic>Molecular chains</topic><topic>Nucleotide sequencing</topic><topic>Polymerase chain reaction</topic><topic>Population decline</topic><topic>Reproduction (biology)</topic><topic>Rivers</topic><topic>Sympatric populations</topic><topic>Taxonomy</topic><topic>Wildlife conservation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dysthe, Joseph C.</creatorcontrib><creatorcontrib>Carim, Kellie J.</creatorcontrib><creatorcontrib>Franklin, Thomas W.</creatorcontrib><creatorcontrib>Kikkert, Dave</creatorcontrib><creatorcontrib>Young, Michael K.</creatorcontrib><creatorcontrib>McKelvey, Kevin S.</creatorcontrib><creatorcontrib>Schwartz, Michael K.</creatorcontrib><collection>CrossRef</collection><collection>Gale In Context: 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Samples</atitle><jtitle>Western North American naturalist</jtitle><date>2018-04-01</date><risdate>2018</risdate><volume>78</volume><issue>1</issue><spage>92</spage><epage>99</epage><pages>92-99</pages><issn>1527-0904</issn><eissn>1944-8341</eissn><abstract>The northern leatherside chub (Lepidomeda copei) is a cyprinid fish native to the Snake River, Green River, and Bonneville basins of the western United States. Population declines prompted the development of a multistate conservation agreement and strategy, which emphasized the need to reliably delineate its current distribution and monitor its status. To facilitate species monitoring, we developed a quantitative PCR assay to detect northern leatherside chub DNA in environmental samples. The assay consistently detected northern leatherside chub DNA in concentrations as low as 2 copies per reaction and did not amplify DNA of potentially sympatric fish species. The assay amplified a synthetic DNA template representing 3 congeneric species: White River spinedace (L.albivallis), Virgin spinedace, (L.mollispinis mollispinis), and Big Spring spinedace, (L.m. pratensis); however, none of these are sympatric with northern leatherside chub. Field tests of the assay accurately reproduced expected patterns of species occupancy.</abstract><cop>Provo</cop><pub>Brigham Young University</pub><doi>10.3398/064.078.0109</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-0191-6112</orcidid><orcidid>https://orcid.org/0000-0002-9622-9146</orcidid><orcidid>https://orcid.org/0000-0002-6790-7841</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; JSTOR Archive Collection A-Z Listing; Alma/SFX Local Collection |
| subjects | Amplification Assaying Basins Biology Creeks & streams Current distribution Cyprinidae Cytochrome Deoxyribonucleic acid DNA DNA sequencing Ecology Endangered & extinct species Field tests Fish Genetic aspects Identification and classification Lepidomeda albivallis Lepidomeda copei Lepidomeda mollispinis mollispinis Lepidomeda mollispinis pratensis Methods Molecular chains Nucleotide sequencing Polymerase chain reaction Population decline Reproduction (biology) Rivers Sympatric populations Taxonomy Wildlife conservation |
| title | Molecular Detection of Northern Leatherside Chub (Lepidomeda copei) DNA in Environmental Samples |
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