Transcriptome profiling of the peripheral blood mononuclear cells following PRRSV vaccination in Pietrain pig
Porcine reproductive and respiratory syndrome (PRRS) is a devastating viral disease affecting swine production, health and welfare throughout the world. Vaccination has been considered as one of the most economic tools for PRRS control. A synergistic action of the innate and the adaptive immune syst...
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description | Porcine reproductive and respiratory syndrome (PRRS) is a devastating viral disease affecting swine production, health and welfare throughout the world. Vaccination has been considered as one of the most economic tools for PRRS control. A synergistic action of the innate and the adaptive immune system of host is essential for developing a durable protective immunity to vaccine antigen. The peripheral blood mononuclear cells (PBMCs) play central role in immune system and are able to display gene expression patterns characteristics for certain infection. Therefore, the current study aimed to investigate the global transcriptome profiles of PBMCs to characterize the innate and the adaptive immune response to PRRS Virus (PRRSV) vaccine in Pietrain pigs. We employed nine Afryrnetrix gene chip porcine gene 1.0 ST array for the transcriptome profiling of PBMCs collected from three female piglets at immediately before (D0), at one (D1) and 28 d (D28) post PRRSV vaccination given at 4 wk (D0) of their age. Two pairwise contrasts were tested to characterize transcriptome alterations associated with the innate immune response (D1 vs. D0) and the adaptive immune response (D28 vs. D0). Normalization and statistical analysis of microarray data was performed with the 'oligo' and 'limmna' R/Bioconductor package. With FDR < 0.05 and log2 fold change ± 1.5 as cut off criteria, 83 and 53 transcripts were found to be differentially expressed in PBMCs during innate and adaptive response, respectively. The microarray expression results were technically validated by qRT-PCR. The gene ontology (GO) terms such as viral life cycle, regulation of lymphocyte activation, cytokine activity and inflammatory response were enriched during the innate immune response. The GO terms enriched during adaptive response includes cytolysis, T cell mediated cytotoxicity, immunoglobulin production. Significant enrichment of cytokine-cytokine receptor interaction, signaling by interleukins, viral mRNA translation. IFN-γ pathway and AP-1 transcription factor network pathways was indicating the involvement of altered genes in the antiviral defense. Network analysis has detected four module were functionally involved with functional network of innate immune transcriptional response and five modules were detected for adaptive immune responses. The innate immune transcriptional network found to be regulated by LCK, STAT3, ATP5B, UBB and RSP17. While TGFβ, IL7R, RAD21, SP1 and GZMB are responsible for coor |
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fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_journals_2038228124</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2038228124</sourcerecordid><originalsourceid>FETCH-proquest_journals_20382281243</originalsourceid><addsrcrecordid>eNqNTksKwjAUDKJg_dzhgetCklqpa1FciopbiTHVyGteTVK9vhE8gKsZ5sNMj2WilGVeiEXRZxnnUuRVJeSQjUJ4cC5kuSwz1hy9ckF720ZqDLSeaovW3YBqiPckmGTdjVcIFyS6QkOOXKfRKA_aIAaoCZHe385uvz-c4KW0tk5FSw6sg5010atEWnubsEGtMJjpD8dstlkfV9s87T47E-L5QZ13yTpLXlRSpsPz4r_UB5BgS5U</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2038228124</pqid></control><display><type>article</type><title>Transcriptome profiling of the peripheral blood mononuclear cells following PRRSV vaccination in Pietrain pig</title><source>AUTh Library subscriptions: Oxford University Press</source><creator>Islam, A ; Neuhoff, C ; Große-Brinkhaus, C ; Pröll, M J ; Uddin, M J ; Rony, S Aqter ; Tesfaye, D ; Tholen, E ; Hölker, M ; Schellander, K</creator><creatorcontrib>Islam, A ; Neuhoff, C ; Große-Brinkhaus, C ; Pröll, M J ; Uddin, M J ; Rony, S Aqter ; Tesfaye, D ; Tholen, E ; Hölker, M ; Schellander, K</creatorcontrib><description>Porcine reproductive and respiratory syndrome (PRRS) is a devastating viral disease affecting swine production, health and welfare throughout the world. Vaccination has been considered as one of the most economic tools for PRRS control. A synergistic action of the innate and the adaptive immune system of host is essential for developing a durable protective immunity to vaccine antigen. The peripheral blood mononuclear cells (PBMCs) play central role in immune system and are able to display gene expression patterns characteristics for certain infection. Therefore, the current study aimed to investigate the global transcriptome profiles of PBMCs to characterize the innate and the adaptive immune response to PRRS Virus (PRRSV) vaccine in Pietrain pigs. We employed nine Afryrnetrix gene chip porcine gene 1.0 ST array for the transcriptome profiling of PBMCs collected from three female piglets at immediately before (D0), at one (D1) and 28 d (D28) post PRRSV vaccination given at 4 wk (D0) of their age. Two pairwise contrasts were tested to characterize transcriptome alterations associated with the innate immune response (D1 vs. D0) and the adaptive immune response (D28 vs. D0). Normalization and statistical analysis of microarray data was performed with the 'oligo' and 'limmna' R/Bioconductor package. With FDR < 0.05 and log2 fold change ± 1.5 as cut off criteria, 83 and 53 transcripts were found to be differentially expressed in PBMCs during innate and adaptive response, respectively. The microarray expression results were technically validated by qRT-PCR. The gene ontology (GO) terms such as viral life cycle, regulation of lymphocyte activation, cytokine activity and inflammatory response were enriched during the innate immune response. The GO terms enriched during adaptive response includes cytolysis, T cell mediated cytotoxicity, immunoglobulin production. Significant enrichment of cytokine-cytokine receptor interaction, signaling by interleukins, viral mRNA translation. IFN-γ pathway and AP-1 transcription factor network pathways was indicating the involvement of altered genes in the antiviral defense. Network analysis has detected four module were functionally involved with functional network of innate immune transcriptional response and five modules were detected for adaptive immune responses. The innate immune transcriptional network found to be regulated by LCK, STAT3, ATP5B, UBB and RSP17. While TGFβ, IL7R, RAD21, SP1 and GZMB are responsible for coordinating the adaptive immune transcriptional response to PRRSV vaccine in PBMCs. Further work is required to determine whether polymorphisms linked to these genes affect the immune response to PRRSV vaccine in pigs.</description><identifier>ISSN: 0021-8812</identifier><identifier>EISSN: 1525-3163</identifier><language>eng</language><publisher>Champaign: Oxford University Press</publisher><subject>Activator protein 1 ; Adaptive control ; Adaptive immunity ; Animal diseases ; Cell activation ; Cytolysis ; Cytotoxicity ; Data processing ; DNA microarrays ; Enrichment ; Gene expression ; Genes ; Hogs ; Immune response ; Immune system ; Immunity ; Immunization ; Inflammation ; Inflammatory response ; Innate immunity ; Interleukin 7 receptors ; Lck protein ; Leukocytes (mononuclear) ; Life cycle engineering ; Life cycles ; Lymphocytes ; Lymphocytes T ; Network analysis ; Peripheral blood mononuclear cells ; Sp1 protein ; Stat3 protein ; Statistical analysis ; Suidae ; Swine ; Swine production ; Toxicity ; Transcription factors ; Vaccination ; Vaccines ; Viral diseases ; Viruses ; γ-Interferon</subject><ispartof>Journal of animal science, 2016-09, Vol.94, p.153-154</ispartof><rights>Copyright Oxford University Press, UK Sep 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Islam, A</creatorcontrib><creatorcontrib>Neuhoff, C</creatorcontrib><creatorcontrib>Große-Brinkhaus, C</creatorcontrib><creatorcontrib>Pröll, M J</creatorcontrib><creatorcontrib>Uddin, M J</creatorcontrib><creatorcontrib>Rony, S Aqter</creatorcontrib><creatorcontrib>Tesfaye, D</creatorcontrib><creatorcontrib>Tholen, E</creatorcontrib><creatorcontrib>Hölker, M</creatorcontrib><creatorcontrib>Schellander, K</creatorcontrib><title>Transcriptome profiling of the peripheral blood mononuclear cells following PRRSV vaccination in Pietrain pig</title><title>Journal of animal science</title><description>Porcine reproductive and respiratory syndrome (PRRS) is a devastating viral disease affecting swine production, health and welfare throughout the world. Vaccination has been considered as one of the most economic tools for PRRS control. A synergistic action of the innate and the adaptive immune system of host is essential for developing a durable protective immunity to vaccine antigen. The peripheral blood mononuclear cells (PBMCs) play central role in immune system and are able to display gene expression patterns characteristics for certain infection. Therefore, the current study aimed to investigate the global transcriptome profiles of PBMCs to characterize the innate and the adaptive immune response to PRRS Virus (PRRSV) vaccine in Pietrain pigs. We employed nine Afryrnetrix gene chip porcine gene 1.0 ST array for the transcriptome profiling of PBMCs collected from three female piglets at immediately before (D0), at one (D1) and 28 d (D28) post PRRSV vaccination given at 4 wk (D0) of their age. Two pairwise contrasts were tested to characterize transcriptome alterations associated with the innate immune response (D1 vs. D0) and the adaptive immune response (D28 vs. D0). Normalization and statistical analysis of microarray data was performed with the 'oligo' and 'limmna' R/Bioconductor package. With FDR < 0.05 and log2 fold change ± 1.5 as cut off criteria, 83 and 53 transcripts were found to be differentially expressed in PBMCs during innate and adaptive response, respectively. The microarray expression results were technically validated by qRT-PCR. The gene ontology (GO) terms such as viral life cycle, regulation of lymphocyte activation, cytokine activity and inflammatory response were enriched during the innate immune response. The GO terms enriched during adaptive response includes cytolysis, T cell mediated cytotoxicity, immunoglobulin production. Significant enrichment of cytokine-cytokine receptor interaction, signaling by interleukins, viral mRNA translation. IFN-γ pathway and AP-1 transcription factor network pathways was indicating the involvement of altered genes in the antiviral defense. Network analysis has detected four module were functionally involved with functional network of innate immune transcriptional response and five modules were detected for adaptive immune responses. The innate immune transcriptional network found to be regulated by LCK, STAT3, ATP5B, UBB and RSP17. While TGFβ, IL7R, RAD21, SP1 and GZMB are responsible for coordinating the adaptive immune transcriptional response to PRRSV vaccine in PBMCs. Further work is required to determine whether polymorphisms linked to these genes affect the immune response to PRRSV vaccine in pigs.</description><subject>Activator protein 1</subject><subject>Adaptive control</subject><subject>Adaptive immunity</subject><subject>Animal diseases</subject><subject>Cell activation</subject><subject>Cytolysis</subject><subject>Cytotoxicity</subject><subject>Data processing</subject><subject>DNA microarrays</subject><subject>Enrichment</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Hogs</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunity</subject><subject>Immunization</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Innate immunity</subject><subject>Interleukin 7 receptors</subject><subject>Lck protein</subject><subject>Leukocytes (mononuclear)</subject><subject>Life cycle engineering</subject><subject>Life cycles</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Network analysis</subject><subject>Peripheral blood mononuclear cells</subject><subject>Sp1 protein</subject><subject>Stat3 protein</subject><subject>Statistical analysis</subject><subject>Suidae</subject><subject>Swine</subject><subject>Swine production</subject><subject>Toxicity</subject><subject>Transcription factors</subject><subject>Vaccination</subject><subject>Vaccines</subject><subject>Viral diseases</subject><subject>Viruses</subject><subject>γ-Interferon</subject><issn>0021-8812</issn><issn>1525-3163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNTksKwjAUDKJg_dzhgetCklqpa1FciopbiTHVyGteTVK9vhE8gKsZ5sNMj2WilGVeiEXRZxnnUuRVJeSQjUJ4cC5kuSwz1hy9ckF720ZqDLSeaovW3YBqiPckmGTdjVcIFyS6QkOOXKfRKA_aIAaoCZHe385uvz-c4KW0tk5FSw6sg5010atEWnubsEGtMJjpD8dstlkfV9s87T47E-L5QZ13yTpLXlRSpsPz4r_UB5BgS5U</recordid><startdate>20160901</startdate><enddate>20160901</enddate><creator>Islam, A</creator><creator>Neuhoff, C</creator><creator>Große-Brinkhaus, C</creator><creator>Pröll, M J</creator><creator>Uddin, M J</creator><creator>Rony, S Aqter</creator><creator>Tesfaye, D</creator><creator>Tholen, E</creator><creator>Hölker, M</creator><creator>Schellander, K</creator><general>Oxford University 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profiling of the peripheral blood mononuclear cells following PRRSV vaccination in Pietrain pig</title><author>Islam, A ; Neuhoff, C ; Große-Brinkhaus, C ; Pröll, M J ; Uddin, M J ; Rony, S Aqter ; Tesfaye, D ; Tholen, E ; Hölker, M ; Schellander, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_20382281243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Activator protein 1</topic><topic>Adaptive control</topic><topic>Adaptive immunity</topic><topic>Animal diseases</topic><topic>Cell activation</topic><topic>Cytolysis</topic><topic>Cytotoxicity</topic><topic>Data processing</topic><topic>DNA microarrays</topic><topic>Enrichment</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Hogs</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immunity</topic><topic>Immunization</topic><topic>Inflammation</topic><topic>Inflammatory response</topic><topic>Innate immunity</topic><topic>Interleukin 7 receptors</topic><topic>Lck protein</topic><topic>Leukocytes (mononuclear)</topic><topic>Life cycle engineering</topic><topic>Life cycles</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Network analysis</topic><topic>Peripheral blood mononuclear cells</topic><topic>Sp1 protein</topic><topic>Stat3 protein</topic><topic>Statistical analysis</topic><topic>Suidae</topic><topic>Swine</topic><topic>Swine production</topic><topic>Toxicity</topic><topic>Transcription factors</topic><topic>Vaccination</topic><topic>Vaccines</topic><topic>Viral diseases</topic><topic>Viruses</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Islam, A</creatorcontrib><creatorcontrib>Neuhoff, C</creatorcontrib><creatorcontrib>Große-Brinkhaus, C</creatorcontrib><creatorcontrib>Pröll, M J</creatorcontrib><creatorcontrib>Uddin, M J</creatorcontrib><creatorcontrib>Rony, S Aqter</creatorcontrib><creatorcontrib>Tesfaye, D</creatorcontrib><creatorcontrib>Tholen, E</creatorcontrib><creatorcontrib>Hölker, M</creatorcontrib><creatorcontrib>Schellander, K</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Career & Technical Education Database</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital 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Collection</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><jtitle>Journal of animal science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Islam, A</au><au>Neuhoff, C</au><au>Große-Brinkhaus, C</au><au>Pröll, M J</au><au>Uddin, M J</au><au>Rony, S Aqter</au><au>Tesfaye, D</au><au>Tholen, E</au><au>Hölker, M</au><au>Schellander, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptome profiling of the peripheral blood mononuclear cells following PRRSV vaccination in Pietrain pig</atitle><jtitle>Journal of animal science</jtitle><date>2016-09-01</date><risdate>2016</risdate><volume>94</volume><spage>153</spage><epage>154</epage><pages>153-154</pages><issn>0021-8812</issn><eissn>1525-3163</eissn><abstract>Porcine reproductive and respiratory syndrome (PRRS) is a devastating viral disease affecting swine production, health and welfare throughout the world. Vaccination has been considered as one of the most economic tools for PRRS control. A synergistic action of the innate and the adaptive immune system of host is essential for developing a durable protective immunity to vaccine antigen. The peripheral blood mononuclear cells (PBMCs) play central role in immune system and are able to display gene expression patterns characteristics for certain infection. Therefore, the current study aimed to investigate the global transcriptome profiles of PBMCs to characterize the innate and the adaptive immune response to PRRS Virus (PRRSV) vaccine in Pietrain pigs. We employed nine Afryrnetrix gene chip porcine gene 1.0 ST array for the transcriptome profiling of PBMCs collected from three female piglets at immediately before (D0), at one (D1) and 28 d (D28) post PRRSV vaccination given at 4 wk (D0) of their age. Two pairwise contrasts were tested to characterize transcriptome alterations associated with the innate immune response (D1 vs. D0) and the adaptive immune response (D28 vs. D0). Normalization and statistical analysis of microarray data was performed with the 'oligo' and 'limmna' R/Bioconductor package. With FDR < 0.05 and log2 fold change ± 1.5 as cut off criteria, 83 and 53 transcripts were found to be differentially expressed in PBMCs during innate and adaptive response, respectively. The microarray expression results were technically validated by qRT-PCR. The gene ontology (GO) terms such as viral life cycle, regulation of lymphocyte activation, cytokine activity and inflammatory response were enriched during the innate immune response. The GO terms enriched during adaptive response includes cytolysis, T cell mediated cytotoxicity, immunoglobulin production. Significant enrichment of cytokine-cytokine receptor interaction, signaling by interleukins, viral mRNA translation. IFN-γ pathway and AP-1 transcription factor network pathways was indicating the involvement of altered genes in the antiviral defense. Network analysis has detected four module were functionally involved with functional network of innate immune transcriptional response and five modules were detected for adaptive immune responses. The innate immune transcriptional network found to be regulated by LCK, STAT3, ATP5B, UBB and RSP17. While TGFβ, IL7R, RAD21, SP1 and GZMB are responsible for coordinating the adaptive immune transcriptional response to PRRSV vaccine in PBMCs. Further work is required to determine whether polymorphisms linked to these genes affect the immune response to PRRSV vaccine in pigs.</abstract><cop>Champaign</cop><pub>Oxford University Press</pub></addata></record> |
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subjects | Activator protein 1 Adaptive control Adaptive immunity Animal diseases Cell activation Cytolysis Cytotoxicity Data processing DNA microarrays Enrichment Gene expression Genes Hogs Immune response Immune system Immunity Immunization Inflammation Inflammatory response Innate immunity Interleukin 7 receptors Lck protein Leukocytes (mononuclear) Life cycle engineering Life cycles Lymphocytes Lymphocytes T Network analysis Peripheral blood mononuclear cells Sp1 protein Stat3 protein Statistical analysis Suidae Swine Swine production Toxicity Transcription factors Vaccination Vaccines Viral diseases Viruses γ-Interferon |
title | Transcriptome profiling of the peripheral blood mononuclear cells following PRRSV vaccination in Pietrain pig |
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