Expression and insecticidal activity of Yersinia pseudotuberculosis and Photorhabdus luminescens toxin complex proteins
Photorhabdus luminescens toxin complex (Tc) has been characterized as a potent three-component insecticidal protein complex. Homologues of genes encoding P. luminescens Tc components have been identified in several other enterobacteria and in Gram-positive bacteria, showing these genes are widesprea...
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| Veröffentlicht in: | Cellular microbiology 2007-10, Vol.9 (10), p.2372-2380 |
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| creator | Pinheiro, Vitor B Ellar, David J |
| description | Photorhabdus luminescens toxin complex (Tc) has been characterized as a potent three-component insecticidal protein complex. Homologues of genes encoding P. luminescens Tc components have been identified in several other enterobacteria and in Gram-positive bacteria, showing these genes are widespread in bacteria. In particular, tc gene homologues have been identified in Yersinia enterocolitica, Yersinia pseudotuberculosis and Yersinia pestis and may have a role in Y. pestis evolution. Y. enterocolitica tc genes have been shown to be active against Manduca sexta larvae. Here, we demonstrate that expression optimization is essential to obtain bioactive P. luminescens Tc proteins and demonstrate that TcaAB and TcdB + TccC are stand-alone toxins against a M. sexta insect model. Moreover, we report that Y. pseudotuberculosis IP32953 Tc proteins are also toxic to M. sexta larvae but do not cross-potentiate as P. luminescens Tc components. |
| doi_str_mv | 10.1111/j.1462-5822.2007.00966.x |
| format | Article |
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Moreover, we report that Y. pseudotuberculosis IP32953 Tc proteins are also toxic to M. sexta larvae but do not cross-potentiate as P. luminescens Tc components.</description><subject>Animals</subject><subject>Bacterial Toxins - biosynthesis</subject><subject>Bacterial Toxins - genetics</subject><subject>Larva - growth & development</subject><subject>Manduca - genetics</subject><subject>Manduca - growth & development</subject><subject>Manduca - metabolism</subject><subject>Photorhabdus - metabolism</subject><subject>Yersinia pseudotuberculosis - metabolism</subject><issn>1462-5814</issn><issn>1462-5822</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtr3DAQx0VoaF79Cqnofd2R5JW9kEtZ8oKUBtocchKyNW60eC1HYyfebx9tdkmv1UUD-j9GP8a4gEyk832ViVzL2byUMpMARQaw0DqbDtjxx8Onj1nkR-yEaAUgdCHEZ3YkinmhFqCP2evl1Eck8qHjtnPcd4T14GvvbMttml78sOGh4Y8YyXfe8p5wdGEYK4z12Aby9G68fwpDiE-2ciPxdlz7DqnGjvgQJt_xOqz7FifexzBgKjljh41tCb_s71P2cHX5Z3kzu_t1fbv8cTerc13omRSqLOdWQTOvKle7KneL9AeHC6WstAoFWBANKmsluLLMC61zRFspEKgbpU7Zt11uKn4ekQazCmPsUqWRoBKFQsgkKneiOgaiiI3po1_buDECzBa4WZktS7PlarbAzTtwMyXr-T5_rNbo_hn3hJPgYid49S1u_jvYLH_epiHZv-7sjQ3G_o2ezMNvCUIBlGl_Uag3ZhubIA</recordid><startdate>200710</startdate><enddate>200710</enddate><creator>Pinheiro, Vitor B</creator><creator>Ellar, David J</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Hindawi Limited</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>200710</creationdate><title>Expression and insecticidal activity of Yersinia pseudotuberculosis and Photorhabdus luminescens toxin complex proteins</title><author>Pinheiro, Vitor B ; Ellar, David J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4676-213885a30f5bbdcdb4d9671de933a2a3e10a01fe3aa20d8847664eeab301e6f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Bacterial Toxins - biosynthesis</topic><topic>Bacterial Toxins - genetics</topic><topic>Larva - growth & development</topic><topic>Manduca - genetics</topic><topic>Manduca - growth & development</topic><topic>Manduca - metabolism</topic><topic>Photorhabdus - metabolism</topic><topic>Yersinia pseudotuberculosis - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pinheiro, Vitor B</creatorcontrib><creatorcontrib>Ellar, David J</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Cellular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pinheiro, Vitor B</au><au>Ellar, David J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and insecticidal activity of Yersinia pseudotuberculosis and Photorhabdus luminescens toxin complex proteins</atitle><jtitle>Cellular microbiology</jtitle><addtitle>Cell Microbiol</addtitle><date>2007-10</date><risdate>2007</risdate><volume>9</volume><issue>10</issue><spage>2372</spage><epage>2380</epage><pages>2372-2380</pages><issn>1462-5814</issn><eissn>1462-5822</eissn><abstract>Photorhabdus luminescens toxin complex (Tc) has been characterized as a potent three-component insecticidal protein complex. Homologues of genes encoding P. luminescens Tc components have been identified in several other enterobacteria and in Gram-positive bacteria, showing these genes are widespread in bacteria. In particular, tc gene homologues have been identified in Yersinia enterocolitica, Yersinia pseudotuberculosis and Yersinia pestis and may have a role in Y. pestis evolution. Y. enterocolitica tc genes have been shown to be active against Manduca sexta larvae. Here, we demonstrate that expression optimization is essential to obtain bioactive P. luminescens Tc proteins and demonstrate that TcaAB and TcdB + TccC are stand-alone toxins against a M. sexta insect model. 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| subjects | Animals Bacterial Toxins - biosynthesis Bacterial Toxins - genetics Larva - growth & development Manduca - genetics Manduca - growth & development Manduca - metabolism Photorhabdus - metabolism Yersinia pseudotuberculosis - metabolism |
| title | Expression and insecticidal activity of Yersinia pseudotuberculosis and Photorhabdus luminescens toxin complex proteins |
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