Effects of dietary leucine levels on growth, tissue protein content and relative expression of genes related to protein synthesis in juvenile gibel carp (Carassius auratus gibelio var. CAS III)
An 8‐week feeding trial was conducted to investigate the impacts of dietary leucine on growth, hepatopancreas and muscle protein content and relative expressions of protein synthesis‐related genes in juvenile gibel carp (5.2 g). Triplicate tanks of fish (22 fish per tank) were fed with a low (0.82%)...
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| Veröffentlicht in: | Aquaculture research 2018-06, Vol.49 (6), p.2240-2248 |
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| creator | Zou, Tao Cao, Shen‐Ping Xu, Wen‐Jie Han, Dong Liu, Hao‐Kun Yang, Yun‐Xia Jin, Jun‐Yan Zhu, Xiao‐Ming Xie, Shou‐Qi |
| description | An 8‐week feeding trial was conducted to investigate the impacts of dietary leucine on growth, hepatopancreas and muscle protein content and relative expressions of protein synthesis‐related genes in juvenile gibel carp (5.2 g). Triplicate tanks of fish (22 fish per tank) were fed with a low (0.82%), middle (1.90%) or high (2.99%) leucine diet to apparent satiation three times a day. The results showed that the weight gain (WG) and feed efficiency (FE) of the fish fed with the 1.90% leucine diet had the highest values compared to those of other two groups (p |
| doi_str_mv | 10.1111/are.13682 |
| format | Article |
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CAS III)</title><source>Wiley Online Library All Journals</source><creator>Zou, Tao ; Cao, Shen‐Ping ; Xu, Wen‐Jie ; Han, Dong ; Liu, Hao‐Kun ; Yang, Yun‐Xia ; Jin, Jun‐Yan ; Zhu, Xiao‐Ming ; Xie, Shou‐Qi</creator><creatorcontrib>Zou, Tao ; Cao, Shen‐Ping ; Xu, Wen‐Jie ; Han, Dong ; Liu, Hao‐Kun ; Yang, Yun‐Xia ; Jin, Jun‐Yan ; Zhu, Xiao‐Ming ; Xie, Shou‐Qi</creatorcontrib><description>An 8‐week feeding trial was conducted to investigate the impacts of dietary leucine on growth, hepatopancreas and muscle protein content and relative expressions of protein synthesis‐related genes in juvenile gibel carp (5.2 g). Triplicate tanks of fish (22 fish per tank) were fed with a low (0.82%), middle (1.90%) or high (2.99%) leucine diet to apparent satiation three times a day. The results showed that the weight gain (WG) and feed efficiency (FE) of the fish fed with the 1.90% leucine diet had the highest values compared to those of other two groups (p < .05). Dietary leucine significantly affected the protein and lipid contents of the hepatopancreas (p < .05), but not muscle or the whole body composition of the fish. The amino acid contents (Arginine, Leucine, Methionine, Phenylalanine, Alanine, Aspartic acid, Glutamic acid and Proline) of the dorsal muscles of fish fed with a 1.90% leucine diet were significantly higher than those fed with a 0.82% leucine diet. Moreover, transcriptional levels of target genes of rapamycin (TOR), ribosomal protein S6 kinase 1 (S6K1), eukaryotic translation initiation factor 4E (eIF4E) and leucyl‐tRNA synthetase (LARS) in the hepatopancreas were significantly up‐regulated (p < .05), but eukaryotic translation initiation factor 4E‐binding protein 2 (4E‐BP2) was significantly down‐regulated (p < .05) with the increasing dietary leucine levels. However, dietary leucine had no effects on the gene expressions of 4E‐BP2 and S6K1 in the muscle of gibel carp. The present results indicate that dietary leucine led to a marked tissue‐specific response on protein content in the muscle and hepatopancreas of gibel carp that is related to the TOR signalling pathway.</description><identifier>ISSN: 1355-557X</identifier><identifier>EISSN: 1365-2109</identifier><identifier>DOI: 10.1111/are.13682</identifier><language>eng</language><publisher>Oxford: Hindawi Limited</publisher><subject>Alanine ; Amino acid composition ; Amino acids ; Arginine ; Aspartic acid ; Body composition ; Body weight gain ; Carp ; Diet ; Feed conversion efficiency ; Feed efficiency ; Feeding experiments ; Fish ; Freshwater fishes ; Gene expression ; Genes ; gibel carp (Carassius auratus gibelio) ; Glutamic acid ; growth performance ; Hepatopancreas ; Initiation factor eIF-4E ; Leucine ; Lipids ; Methionine ; Muscles ; Phenylalanine ; Proline ; Protein biosynthesis ; Protein synthesis ; Proteins ; Rapamycin ; Ribosomal protein S6 ; Satiety ; Signal transduction ; Tanks ; Tissue ; TOR pathway ; Transcription ; tRNA</subject><ispartof>Aquaculture research, 2018-06, Vol.49 (6), p.2240-2248</ispartof><rights>2018 John Wiley & Sons Ltd</rights><rights>Copyright © 2018 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2972-76670f7ca5272fc17fcc111e48188c8bbb0cc7c58681a5db16b38ea2ad95e1db3</citedby><cites>FETCH-LOGICAL-c2972-76670f7ca5272fc17fcc111e48188c8bbb0cc7c58681a5db16b38ea2ad95e1db3</cites><orcidid>0000-0002-8411-3736 ; 0000-0002-7139-687X ; 0000-0002-3242-7362 ; 0000-0002-3212-9824</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fare.13682$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fare.13682$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids></links><search><creatorcontrib>Zou, Tao</creatorcontrib><creatorcontrib>Cao, Shen‐Ping</creatorcontrib><creatorcontrib>Xu, Wen‐Jie</creatorcontrib><creatorcontrib>Han, Dong</creatorcontrib><creatorcontrib>Liu, Hao‐Kun</creatorcontrib><creatorcontrib>Yang, Yun‐Xia</creatorcontrib><creatorcontrib>Jin, Jun‐Yan</creatorcontrib><creatorcontrib>Zhu, Xiao‐Ming</creatorcontrib><creatorcontrib>Xie, Shou‐Qi</creatorcontrib><title>Effects of dietary leucine levels on growth, tissue protein content and relative expression of genes related to protein synthesis in juvenile gibel carp (Carassius auratus gibelio var. CAS III)</title><title>Aquaculture research</title><description>An 8‐week feeding trial was conducted to investigate the impacts of dietary leucine on growth, hepatopancreas and muscle protein content and relative expressions of protein synthesis‐related genes in juvenile gibel carp (5.2 g). Triplicate tanks of fish (22 fish per tank) were fed with a low (0.82%), middle (1.90%) or high (2.99%) leucine diet to apparent satiation three times a day. The results showed that the weight gain (WG) and feed efficiency (FE) of the fish fed with the 1.90% leucine diet had the highest values compared to those of other two groups (p < .05). Dietary leucine significantly affected the protein and lipid contents of the hepatopancreas (p < .05), but not muscle or the whole body composition of the fish. The amino acid contents (Arginine, Leucine, Methionine, Phenylalanine, Alanine, Aspartic acid, Glutamic acid and Proline) of the dorsal muscles of fish fed with a 1.90% leucine diet were significantly higher than those fed with a 0.82% leucine diet. Moreover, transcriptional levels of target genes of rapamycin (TOR), ribosomal protein S6 kinase 1 (S6K1), eukaryotic translation initiation factor 4E (eIF4E) and leucyl‐tRNA synthetase (LARS) in the hepatopancreas were significantly up‐regulated (p < .05), but eukaryotic translation initiation factor 4E‐binding protein 2 (4E‐BP2) was significantly down‐regulated (p < .05) with the increasing dietary leucine levels. However, dietary leucine had no effects on the gene expressions of 4E‐BP2 and S6K1 in the muscle of gibel carp. The present results indicate that dietary leucine led to a marked tissue‐specific response on protein content in the muscle and hepatopancreas of gibel carp that is related to the TOR signalling pathway.</description><subject>Alanine</subject><subject>Amino acid composition</subject><subject>Amino acids</subject><subject>Arginine</subject><subject>Aspartic acid</subject><subject>Body composition</subject><subject>Body weight gain</subject><subject>Carp</subject><subject>Diet</subject><subject>Feed conversion efficiency</subject><subject>Feed efficiency</subject><subject>Feeding experiments</subject><subject>Fish</subject><subject>Freshwater fishes</subject><subject>Gene expression</subject><subject>Genes</subject><subject>gibel carp (Carassius auratus gibelio)</subject><subject>Glutamic acid</subject><subject>growth performance</subject><subject>Hepatopancreas</subject><subject>Initiation factor eIF-4E</subject><subject>Leucine</subject><subject>Lipids</subject><subject>Methionine</subject><subject>Muscles</subject><subject>Phenylalanine</subject><subject>Proline</subject><subject>Protein biosynthesis</subject><subject>Protein synthesis</subject><subject>Proteins</subject><subject>Rapamycin</subject><subject>Ribosomal protein S6</subject><subject>Satiety</subject><subject>Signal transduction</subject><subject>Tanks</subject><subject>Tissue</subject><subject>TOR pathway</subject><subject>Transcription</subject><subject>tRNA</subject><issn>1355-557X</issn><issn>1365-2109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1UVtLwzAULqLgvDz4Dw744sDOJF3a7nGMqQNB8AK-lTQ93TJqOpN0up_nP_NoxTfz8iV8l3DOF0VnnI04nSvlcMSTNBd70YBQxoKzyf73XcpYyuzlMDryfs0YH7OED6LPeV2jDh7aGiqDQbkdNNhpY5Fwiw0xFpaufQ-rSwjG-w5h49qAxoJubUAbQNkKHDYqmC0Cfmwcem_IRplLtOh7EisI7Z_X72xYoTce6LHutmhNg7A0JTagldvAxUw5RTmdB9U5FQh_WNPCVrkRzKaPsFgshifRQa0aj6e_eBw9X8-fZrfx3f3NYja9i7WYZCLO0jRjdaaVFJmoNc9qrWlhOM55nuu8LEumdaZlnuZcyarkaZnkqISqJhJ5VSbH0XmfSxO8dehDsW47Z-nLQrBECskmIiXVsFdp13rvsC42zrzSVgvOiu-GCmqo-GmItFe99p1G3_0vLKYP897xBcS2lmE</recordid><startdate>201806</startdate><enddate>201806</enddate><creator>Zou, Tao</creator><creator>Cao, Shen‐Ping</creator><creator>Xu, Wen‐Jie</creator><creator>Han, Dong</creator><creator>Liu, Hao‐Kun</creator><creator>Yang, Yun‐Xia</creator><creator>Jin, Jun‐Yan</creator><creator>Zhu, Xiao‐Ming</creator><creator>Xie, Shou‐Qi</creator><general>Hindawi Limited</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0002-8411-3736</orcidid><orcidid>https://orcid.org/0000-0002-7139-687X</orcidid><orcidid>https://orcid.org/0000-0002-3242-7362</orcidid><orcidid>https://orcid.org/0000-0002-3212-9824</orcidid></search><sort><creationdate>201806</creationdate><title>Effects of dietary leucine levels on growth, tissue protein content and relative expression of genes related to protein synthesis in juvenile gibel carp (Carassius auratus gibelio var. CAS III)</title><author>Zou, Tao ; Cao, Shen‐Ping ; Xu, Wen‐Jie ; Han, Dong ; Liu, Hao‐Kun ; Yang, Yun‐Xia ; Jin, Jun‐Yan ; Zhu, Xiao‐Ming ; Xie, Shou‐Qi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2972-76670f7ca5272fc17fcc111e48188c8bbb0cc7c58681a5db16b38ea2ad95e1db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Alanine</topic><topic>Amino acid composition</topic><topic>Amino acids</topic><topic>Arginine</topic><topic>Aspartic acid</topic><topic>Body composition</topic><topic>Body weight gain</topic><topic>Carp</topic><topic>Diet</topic><topic>Feed conversion efficiency</topic><topic>Feed efficiency</topic><topic>Feeding experiments</topic><topic>Fish</topic><topic>Freshwater fishes</topic><topic>Gene expression</topic><topic>Genes</topic><topic>gibel carp (Carassius auratus gibelio)</topic><topic>Glutamic acid</topic><topic>growth performance</topic><topic>Hepatopancreas</topic><topic>Initiation factor eIF-4E</topic><topic>Leucine</topic><topic>Lipids</topic><topic>Methionine</topic><topic>Muscles</topic><topic>Phenylalanine</topic><topic>Proline</topic><topic>Protein biosynthesis</topic><topic>Protein synthesis</topic><topic>Proteins</topic><topic>Rapamycin</topic><topic>Ribosomal protein S6</topic><topic>Satiety</topic><topic>Signal transduction</topic><topic>Tanks</topic><topic>Tissue</topic><topic>TOR pathway</topic><topic>Transcription</topic><topic>tRNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zou, Tao</creatorcontrib><creatorcontrib>Cao, Shen‐Ping</creatorcontrib><creatorcontrib>Xu, Wen‐Jie</creatorcontrib><creatorcontrib>Han, Dong</creatorcontrib><creatorcontrib>Liu, Hao‐Kun</creatorcontrib><creatorcontrib>Yang, Yun‐Xia</creatorcontrib><creatorcontrib>Jin, Jun‐Yan</creatorcontrib><creatorcontrib>Zhu, Xiao‐Ming</creatorcontrib><creatorcontrib>Xie, Shou‐Qi</creatorcontrib><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Aquaculture research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zou, Tao</au><au>Cao, Shen‐Ping</au><au>Xu, Wen‐Jie</au><au>Han, Dong</au><au>Liu, Hao‐Kun</au><au>Yang, Yun‐Xia</au><au>Jin, Jun‐Yan</au><au>Zhu, Xiao‐Ming</au><au>Xie, Shou‐Qi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of dietary leucine levels on growth, tissue protein content and relative expression of genes related to protein synthesis in juvenile gibel carp (Carassius auratus gibelio var. CAS III)</atitle><jtitle>Aquaculture research</jtitle><date>2018-06</date><risdate>2018</risdate><volume>49</volume><issue>6</issue><spage>2240</spage><epage>2248</epage><pages>2240-2248</pages><issn>1355-557X</issn><eissn>1365-2109</eissn><abstract>An 8‐week feeding trial was conducted to investigate the impacts of dietary leucine on growth, hepatopancreas and muscle protein content and relative expressions of protein synthesis‐related genes in juvenile gibel carp (5.2 g). Triplicate tanks of fish (22 fish per tank) were fed with a low (0.82%), middle (1.90%) or high (2.99%) leucine diet to apparent satiation three times a day. The results showed that the weight gain (WG) and feed efficiency (FE) of the fish fed with the 1.90% leucine diet had the highest values compared to those of other two groups (p < .05). Dietary leucine significantly affected the protein and lipid contents of the hepatopancreas (p < .05), but not muscle or the whole body composition of the fish. The amino acid contents (Arginine, Leucine, Methionine, Phenylalanine, Alanine, Aspartic acid, Glutamic acid and Proline) of the dorsal muscles of fish fed with a 1.90% leucine diet were significantly higher than those fed with a 0.82% leucine diet. Moreover, transcriptional levels of target genes of rapamycin (TOR), ribosomal protein S6 kinase 1 (S6K1), eukaryotic translation initiation factor 4E (eIF4E) and leucyl‐tRNA synthetase (LARS) in the hepatopancreas were significantly up‐regulated (p < .05), but eukaryotic translation initiation factor 4E‐binding protein 2 (4E‐BP2) was significantly down‐regulated (p < .05) with the increasing dietary leucine levels. However, dietary leucine had no effects on the gene expressions of 4E‐BP2 and S6K1 in the muscle of gibel carp. The present results indicate that dietary leucine led to a marked tissue‐specific response on protein content in the muscle and hepatopancreas of gibel carp that is related to the TOR signalling pathway.</abstract><cop>Oxford</cop><pub>Hindawi Limited</pub><doi>10.1111/are.13682</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-8411-3736</orcidid><orcidid>https://orcid.org/0000-0002-7139-687X</orcidid><orcidid>https://orcid.org/0000-0002-3242-7362</orcidid><orcidid>https://orcid.org/0000-0002-3212-9824</orcidid></addata></record> |
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| subjects | Alanine Amino acid composition Amino acids Arginine Aspartic acid Body composition Body weight gain Carp Diet Feed conversion efficiency Feed efficiency Feeding experiments Fish Freshwater fishes Gene expression Genes gibel carp (Carassius auratus gibelio) Glutamic acid growth performance Hepatopancreas Initiation factor eIF-4E Leucine Lipids Methionine Muscles Phenylalanine Proline Protein biosynthesis Protein synthesis Proteins Rapamycin Ribosomal protein S6 Satiety Signal transduction Tanks Tissue TOR pathway Transcription tRNA |
| title | Effects of dietary leucine levels on growth, tissue protein content and relative expression of genes related to protein synthesis in juvenile gibel carp (Carassius auratus gibelio var. CAS III) |
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