A Sortase A-Immobilized Mesoporous Hollow Carbon Sphere-Based Biosensor for Detection of Gram-Positive Bacteria
A sensor based on mesoporous carbon materials immobilized with sortase A (SrtA) for determination of Staphylococcus aureus ( S. aureus ) is reported. To prepare the biosensor, we first synthesized carboxyl-functionalized mesoporous hollow carbon spheres, then applied them as carriers for immobilizat...
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| Veröffentlicht in: | Journal of electronic materials 2018-07, Vol.47 (7), p.4124-4135 |
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| creator | Wang, Hongsu Luo, Ruiping Chen, Yang Si, Qi Niu, Xiaodi |
| description | A sensor based on mesoporous carbon materials immobilized with sortase A (SrtA) for determination of
Staphylococcus aureus
(
S. aureus
) is reported. To prepare the biosensor, we first synthesized carboxyl-functionalized mesoporous hollow carbon spheres, then applied them as carriers for immobilization of SrtA. Based on the catalytic mechanism of SrtA, a highly sensitive, inexpensive, and rapid method was developed for
S. aureus
detection. The sensor showed a linear response in the bacterial concentration range of 0.125 × 10
2
colony-forming units (CFU) mL
−1
to 2.5 × 10
2
CFU mL
−1
, with detection limit as low as 9.0 CFU mL
−1
. The method was successfully used for quantitative detection of
S. aureus
in whole milk samples, giving results similar to experimental results obtained from the plate counting method. This biosensor could also be used to detect other Gram-positive bacteria that secrete SrtA. |
| doi_str_mv | 10.1007/s11664-018-6308-4 |
| format | Article |
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Staphylococcus aureus
(
S. aureus
) is reported. To prepare the biosensor, we first synthesized carboxyl-functionalized mesoporous hollow carbon spheres, then applied them as carriers for immobilization of SrtA. Based on the catalytic mechanism of SrtA, a highly sensitive, inexpensive, and rapid method was developed for
S. aureus
detection. The sensor showed a linear response in the bacterial concentration range of 0.125 × 10
2
colony-forming units (CFU) mL
−1
to 2.5 × 10
2
CFU mL
−1
, with detection limit as low as 9.0 CFU mL
−1
. The method was successfully used for quantitative detection of
S. aureus
in whole milk samples, giving results similar to experimental results obtained from the plate counting method. This biosensor could also be used to detect other Gram-positive bacteria that secrete SrtA.</description><identifier>ISSN: 0361-5235</identifier><identifier>EISSN: 1543-186X</identifier><identifier>DOI: 10.1007/s11664-018-6308-4</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Bacteria ; Biosensors ; Carbon ; Catalysis ; Characterization and Evaluation of Materials ; Chemistry and Materials Science ; Electronics and Microelectronics ; Gram-positive bacteria ; Instrumentation ; Materials Science ; Optical and Electronic Materials ; Solid State Physics</subject><ispartof>Journal of electronic materials, 2018-07, Vol.47 (7), p.4124-4135</ispartof><rights>The Minerals, Metals & Materials Society 2018</rights><rights>Journal of Electronic Materials is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-2d4b9a1b06dca0cb5ea52e741b7f1a74076c2ea8924da1e4508974b5052110783</citedby><cites>FETCH-LOGICAL-c353t-2d4b9a1b06dca0cb5ea52e741b7f1a74076c2ea8924da1e4508974b5052110783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11664-018-6308-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11664-018-6308-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Wang, Hongsu</creatorcontrib><creatorcontrib>Luo, Ruiping</creatorcontrib><creatorcontrib>Chen, Yang</creatorcontrib><creatorcontrib>Si, Qi</creatorcontrib><creatorcontrib>Niu, Xiaodi</creatorcontrib><title>A Sortase A-Immobilized Mesoporous Hollow Carbon Sphere-Based Biosensor for Detection of Gram-Positive Bacteria</title><title>Journal of electronic materials</title><addtitle>Journal of Elec Materi</addtitle><description>A sensor based on mesoporous carbon materials immobilized with sortase A (SrtA) for determination of
Staphylococcus aureus
(
S. aureus
) is reported. To prepare the biosensor, we first synthesized carboxyl-functionalized mesoporous hollow carbon spheres, then applied them as carriers for immobilization of SrtA. Based on the catalytic mechanism of SrtA, a highly sensitive, inexpensive, and rapid method was developed for
S. aureus
detection. The sensor showed a linear response in the bacterial concentration range of 0.125 × 10
2
colony-forming units (CFU) mL
−1
to 2.5 × 10
2
CFU mL
−1
, with detection limit as low as 9.0 CFU mL
−1
. The method was successfully used for quantitative detection of
S. aureus
in whole milk samples, giving results similar to experimental results obtained from the plate counting method. This biosensor could also be used to detect other Gram-positive bacteria that secrete SrtA.</description><subject>Bacteria</subject><subject>Biosensors</subject><subject>Carbon</subject><subject>Catalysis</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry and Materials Science</subject><subject>Electronics and Microelectronics</subject><subject>Gram-positive bacteria</subject><subject>Instrumentation</subject><subject>Materials Science</subject><subject>Optical and Electronic Materials</subject><subject>Solid State Physics</subject><issn>0361-5235</issn><issn>1543-186X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kE1Lw0AQQBdRsFZ_gLcFz6s7-5GPY1u1LVQUquBt2SQTTUmycTdV9NcbieDJwzCX92bgEXIO_BI4j68CQBQpxiFhkeQJUwdkAlpJBkn0fEgmXEbAtJD6mJyEsOMcNCQwIW5Gt873NiCdsXXTuKyqqy8s6B0G1znv9oGuXF27D7qwPnMt3Xav6JHNB6Wg88oFbIPztBzmGnvM-2qAXEmX3jbswYWqr96Rzm3eo6_sKTkqbR3w7HdPydPtzeNixTb3y_VitmG51LJnolBZaiHjUZFbnmcarRYYK8jiEmyseBzlAm2SClVYQKV5ksYq01wLAB4nckouxrudd297DL3Zub1vh5dGcClBpQpgoGCkcu9C8FiazleN9Z8GuPnpasauZuhqfroaNThidMLAti_o_y7_L30DwIx6og</recordid><startdate>20180701</startdate><enddate>20180701</enddate><creator>Wang, Hongsu</creator><creator>Luo, Ruiping</creator><creator>Chen, Yang</creator><creator>Si, Qi</creator><creator>Niu, Xiaodi</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7XB</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>L6V</scope><scope>M2O</scope><scope>M2P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>P5Z</scope><scope>P62</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>S0X</scope></search><sort><creationdate>20180701</creationdate><title>A Sortase A-Immobilized Mesoporous Hollow Carbon Sphere-Based Biosensor for Detection of Gram-Positive Bacteria</title><author>Wang, Hongsu ; Luo, Ruiping ; Chen, Yang ; Si, Qi ; Niu, Xiaodi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-2d4b9a1b06dca0cb5ea52e741b7f1a74076c2ea8924da1e4508974b5052110783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Bacteria</topic><topic>Biosensors</topic><topic>Carbon</topic><topic>Catalysis</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry and Materials Science</topic><topic>Electronics and Microelectronics</topic><topic>Gram-positive bacteria</topic><topic>Instrumentation</topic><topic>Materials Science</topic><topic>Optical and Electronic Materials</topic><topic>Solid State Physics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Hongsu</creatorcontrib><creatorcontrib>Luo, Ruiping</creatorcontrib><creatorcontrib>Chen, Yang</creatorcontrib><creatorcontrib>Si, Qi</creatorcontrib><creatorcontrib>Niu, Xiaodi</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>ProQuest Engineering Collection</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><jtitle>Journal of electronic materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Hongsu</au><au>Luo, Ruiping</au><au>Chen, Yang</au><au>Si, Qi</au><au>Niu, Xiaodi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Sortase A-Immobilized Mesoporous Hollow Carbon Sphere-Based Biosensor for Detection of Gram-Positive Bacteria</atitle><jtitle>Journal of electronic materials</jtitle><stitle>Journal of Elec Materi</stitle><date>2018-07-01</date><risdate>2018</risdate><volume>47</volume><issue>7</issue><spage>4124</spage><epage>4135</epage><pages>4124-4135</pages><issn>0361-5235</issn><eissn>1543-186X</eissn><abstract>A sensor based on mesoporous carbon materials immobilized with sortase A (SrtA) for determination of
Staphylococcus aureus
(
S. aureus
) is reported. To prepare the biosensor, we first synthesized carboxyl-functionalized mesoporous hollow carbon spheres, then applied them as carriers for immobilization of SrtA. Based on the catalytic mechanism of SrtA, a highly sensitive, inexpensive, and rapid method was developed for
S. aureus
detection. The sensor showed a linear response in the bacterial concentration range of 0.125 × 10
2
colony-forming units (CFU) mL
−1
to 2.5 × 10
2
CFU mL
−1
, with detection limit as low as 9.0 CFU mL
−1
. The method was successfully used for quantitative detection of
S. aureus
in whole milk samples, giving results similar to experimental results obtained from the plate counting method. This biosensor could also be used to detect other Gram-positive bacteria that secrete SrtA.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s11664-018-6308-4</doi><tpages>12</tpages></addata></record> |
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| subjects | Bacteria Biosensors Carbon Catalysis Characterization and Evaluation of Materials Chemistry and Materials Science Electronics and Microelectronics Gram-positive bacteria Instrumentation Materials Science Optical and Electronic Materials Solid State Physics |
| title | A Sortase A-Immobilized Mesoporous Hollow Carbon Sphere-Based Biosensor for Detection of Gram-Positive Bacteria |
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