Gas chromatography-mass spectrometric determination of components of leaves of Aegle marmelos and Psidium guajava and seeds of Nigella sativa and correlation with In vitro antioxidant activity
Objective: The aim of this study is to analyze the volatile components of the leaves of Aegle marmelos, Psidium guajava, and seeds of Nigella sativa, using gas chromatography-mass spectrometric (GC-MS) and correlate with their in vitro antioxidant activity. Materials and Methods: Plant material was...
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| Veröffentlicht in: | Pharmacognosy research 2018-04, Vol.10 (2), p.230-235 |
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| creator | Jayachandran Nair, C Ahamad, Sayeed Khan, Washim Anjum, Varisha Mathur, Rajani |
| description | Objective: The aim of this study is to analyze the volatile components of the leaves of Aegle marmelos, Psidium guajava, and seeds of Nigella sativa, using gas chromatography-mass spectrometric (GC-MS) and correlate with their in vitro antioxidant activity. Materials and Methods: Plant material was analyzed for heavy metal content (HMC), pesticide residue (PR), and the presence of microbes/fungi. Following hydrodistillation, the volatile components (AM, PG, and NS) were subjected to GC-MS analysis and in vitro antioxidant assay (2,2-diphenyl-1-picrylhydrazyl). Results: The plant material passed the test for HMC, PR, microbial, and fungal contamination. Using GC-MS, the number of compounds identified in AM, PG, and NS were 62, 46, and 58, respectively. The antioxidant activity of AM, PG, and NS was 98.538%, 98.955%, and 97.755%, respectively. Conclusion: GC-MS-based methods can be successfully utilized for phytochemical profiling and standardization of plant material.
Abbreviations Used: AM: Volatile oil of Aegle marmelos Correa.; GC-MS: Gas chromatography-mass spectrometry; HMC: Heavy metal content; NS: Volatile oil of Nigella sativa Linn.; PG: Volatile oil of Psidium guajava Linn.; PR: Pesticide residue. |
| doi_str_mv | 10.4103/pr.pr_93_17 |
| format | Article |
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Abbreviations Used: AM: Volatile oil of Aegle marmelos Correa.; GC-MS: Gas chromatography-mass spectrometry; HMC: Heavy metal content; NS: Volatile oil of Nigella sativa Linn.; PG: Volatile oil of Psidium guajava Linn.; PR: Pesticide residue.</description><identifier>ISSN: 0974-8490</identifier><identifier>ISSN: 0976-4836</identifier><identifier>EISSN: 0974-8490</identifier><identifier>DOI: 10.4103/pr.pr_93_17</identifier><language>eng</language><publisher>Bangalore: Wolters Kluwer India Pvt. Ltd</publisher><subject>Analysis ; Antioxidants ; Antioxidants (Nutrients) ; Arsenic ; Biphenyl (Compound) ; Chromatography ; Flavonoids ; Gas chromatography ; Heavy metal content ; Herbal medicine ; Laboratories ; Mass spectrometry ; Natural products ; Pesticides ; Pharmaceutical sciences ; Pharmacology ; Pharmacy ; Potassium ; Scientific imaging ; Seeds ; Standardization</subject><ispartof>Pharmacognosy research, 2018-04, Vol.10 (2), p.230-235</ispartof><rights>COPYRIGHT 2018 Medknow Publications and Media Pvt. Ltd.</rights><rights>Copyright Medknow Publications & Media Pvt. Ltd. Apr/Jun 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386o-92d7be085b70b78a1edfcc4fb5d967899175fb29996aae342a712c0ffe622e323</citedby><cites>FETCH-LOGICAL-c386o-92d7be085b70b78a1edfcc4fb5d967899175fb29996aae342a712c0ffe622e323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Jayachandran Nair, C</creatorcontrib><creatorcontrib>Ahamad, Sayeed</creatorcontrib><creatorcontrib>Khan, Washim</creatorcontrib><creatorcontrib>Anjum, Varisha</creatorcontrib><creatorcontrib>Mathur, Rajani</creatorcontrib><title>Gas chromatography-mass spectrometric determination of components of leaves of Aegle marmelos and Psidium guajava and seeds of Nigella sativa and correlation with In vitro antioxidant activity</title><title>Pharmacognosy research</title><description>Objective: The aim of this study is to analyze the volatile components of the leaves of Aegle marmelos, Psidium guajava, and seeds of Nigella sativa, using gas chromatography-mass spectrometric (GC-MS) and correlate with their in vitro antioxidant activity. Materials and Methods: Plant material was analyzed for heavy metal content (HMC), pesticide residue (PR), and the presence of microbes/fungi. Following hydrodistillation, the volatile components (AM, PG, and NS) were subjected to GC-MS analysis and in vitro antioxidant assay (2,2-diphenyl-1-picrylhydrazyl). Results: The plant material passed the test for HMC, PR, microbial, and fungal contamination. Using GC-MS, the number of compounds identified in AM, PG, and NS were 62, 46, and 58, respectively. The antioxidant activity of AM, PG, and NS was 98.538%, 98.955%, and 97.755%, respectively. Conclusion: GC-MS-based methods can be successfully utilized for phytochemical profiling and standardization of plant material.
Abbreviations Used: AM: Volatile oil of Aegle marmelos Correa.; GC-MS: Gas chromatography-mass spectrometry; HMC: Heavy metal content; NS: Volatile oil of Nigella sativa Linn.; PG: Volatile oil of Psidium guajava Linn.; PR: Pesticide residue.</description><subject>Analysis</subject><subject>Antioxidants</subject><subject>Antioxidants (Nutrients)</subject><subject>Arsenic</subject><subject>Biphenyl (Compound)</subject><subject>Chromatography</subject><subject>Flavonoids</subject><subject>Gas chromatography</subject><subject>Heavy metal content</subject><subject>Herbal medicine</subject><subject>Laboratories</subject><subject>Mass spectrometry</subject><subject>Natural products</subject><subject>Pesticides</subject><subject>Pharmaceutical sciences</subject><subject>Pharmacology</subject><subject>Pharmacy</subject><subject>Potassium</subject><subject>Scientific imaging</subject><subject>Seeds</subject><subject>Standardization</subject><issn>0974-8490</issn><issn>0976-4836</issn><issn>0974-8490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNptUk1v1DAQjRBIVKUn_oAljiiLP7JJfFxVUCpVwAHOlmOPs97acbCdXfbf8dNwd1sBUu3DzLx5b8aacVW9JXjVEMw-zHE1R8GZIN2L6gLzrqn7huOX__ivq6uUdrgcxilvyEX1-0YmpLYxeJnDGOW8PdZepoTSDCoXGHK0CmnIEL2dZLZhQsEgFfwcJphyeogcyD2cvA2MDpCX0YMLCclJo2_Jart4NC5yJ_fyhCUAfeJ_sSM4J1EqlR9zKsQI7tzpYPMW3U5ob8tbSraAv6wuFklVBDYf31SvjHQJrh7tZfXj08fv15_ru683t9ebu1qxvg01p7obAPfrocND10sC2ijVmGGtedv1nJNubQbKOW-lBNZQ2RGqsDHQUgqMssvq3bnuHMPPBVIWu7DEqbQUFDNMCC41_rJG6UDYyYQcpfI2KbFZsxa3LcZtYa2eYZWrwVtVxmpswf8TvD8LVAwpRTBijrYM-SgIFg_LL7F4Wn5hb87sQ3BlbeneLQeIwoO-n8LhOYmgDIvyE8TTT2B_ABAQviQ</recordid><startdate>20180401</startdate><enddate>20180401</enddate><creator>Jayachandran Nair, C</creator><creator>Ahamad, Sayeed</creator><creator>Khan, Washim</creator><creator>Anjum, Varisha</creator><creator>Mathur, Rajani</creator><general>Wolters Kluwer India Pvt. 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Materials and Methods: Plant material was analyzed for heavy metal content (HMC), pesticide residue (PR), and the presence of microbes/fungi. Following hydrodistillation, the volatile components (AM, PG, and NS) were subjected to GC-MS analysis and in vitro antioxidant assay (2,2-diphenyl-1-picrylhydrazyl). Results: The plant material passed the test for HMC, PR, microbial, and fungal contamination. Using GC-MS, the number of compounds identified in AM, PG, and NS were 62, 46, and 58, respectively. The antioxidant activity of AM, PG, and NS was 98.538%, 98.955%, and 97.755%, respectively. Conclusion: GC-MS-based methods can be successfully utilized for phytochemical profiling and standardization of plant material.
Abbreviations Used: AM: Volatile oil of Aegle marmelos Correa.; GC-MS: Gas chromatography-mass spectrometry; HMC: Heavy metal content; NS: Volatile oil of Nigella sativa Linn.; PG: Volatile oil of Psidium guajava Linn.; PR: Pesticide residue.</abstract><cop>Bangalore</cop><pub>Wolters Kluwer India Pvt. Ltd</pub><doi>10.4103/pr.pr_93_17</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Analysis Antioxidants Antioxidants (Nutrients) Arsenic Biphenyl (Compound) Chromatography Flavonoids Gas chromatography Heavy metal content Herbal medicine Laboratories Mass spectrometry Natural products Pesticides Pharmaceutical sciences Pharmacology Pharmacy Potassium Scientific imaging Seeds Standardization |
| title | Gas chromatography-mass spectrometric determination of components of leaves of Aegle marmelos and Psidium guajava and seeds of Nigella sativa and correlation with In vitro antioxidant activity |
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