GC/MS analysis and characterization of 2‐Hexadecen‐1‐ol and beta sitosterol from Schimpera arabica extract for its bioactive potential as antioxidant and antimicrobial
Aims We evaluated the in vitro antioxidant and antimicrobial potential of Schimpera arabica extract and fractions (2‐Hexadecen‐1‐ol and beta sitosterol). Methods and Results Free radical scavenging activity was estimated by using ABTS and DPPH methods, reducing power and iron chelation was studied t...
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| Veröffentlicht in: | Journal of applied microbiology 2018-05, Vol.124 (5), p.1082-1091 |
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| creator | Hidayathulla, S. Shahat, A.A. Ahamad, S.R. Al Moqbil, A.A.N. Alsaid, M.S. Divakar, D.D. |
| description | Aims
We evaluated the in vitro antioxidant and antimicrobial potential of Schimpera arabica extract and fractions (2‐Hexadecen‐1‐ol and beta sitosterol).
Methods and Results
Free radical scavenging activity was estimated by using ABTS and DPPH methods, reducing power and iron chelation was studied to investigate antioxidant potential of the extracts. Well‐diffusion method was utilized to evaluate the antimicrobial activity of the extracts. Gas chromatography‐mass spectroscopy analysis of the extract revealed the presence of 27 compounds. Phenolic and flavonoid contents were found in high amount (7·6–18 mg GAE, mg g−1; 3·2–10·8 mg catechin, mg g−1) in dry plant material.
Conclusions
Among all the extracts, 2‐Hexadecen‐1‐ol and beta sitosterol were the major constituents of crude extract and exhibited significant antioxidant potential as well antimicrobial activity; however, all other fractions also exhibited fare degree of antioxidant potential. 93% DPPH was scavenged by 100 μg ml−1 of the ethyl acetate fraction; in ABTS assay chelation was 86·2 and 88% in ferric chloride reducing power assay.
Significance and Impact of the Study
Schimpera arabica is naturally present and used in food preparation in most Arabian nations. The characterization of S. arabica would provide an insight into its medicinal use as home remedial for bacterial infection and as antioxidant. Significant and comparable antimicrobial activity was demonstrated by both 2‐Hexadecen‐1‐ol and beta sitosterol fractions. The results suggest that S. arabica contain a good amount of natural antioxidants of medicinal importance. However, further investigations are needed to characterize the further activity of 2‐Hexadecen‐1‐ol and beta sitosterol in vivo. |
| doi_str_mv | 10.1111/jam.13704 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2025313088</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2025313088</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3534-2c35f4c070482ebd2bf71c635d75d795b07cec6347d71d8b40ed98d8e9d7a9883</originalsourceid><addsrcrecordid>eNp1kUtOHDEQhq0IFAiw4ALIEqssmvGju929RCMeiUBZkKxbflQLj6bHE9uTzGTFEbhILsVJqOmB7GLZqirr8-9S_YSccnbBcU1merjgUrHyAznksq4KUSuxN-ZlUTElDsinlGaMccmq-iM5EK2saiGbQ_L3Zjq5f6B6oeeb5BMmjtpHHbXNEP0fnX1Y0NBT8fL0fAtr7cDCAnOOJ8xH3EDWNPkcEj7Buz6GgT7YRz8sIWqKWsZbTWGdt6q0D5H6nKjxAUv_C-gyZFhkr1Fu2wB-ufYO46i-rQdvYzAIHJP9Xs8TnLzFI_Lj-ur79La4-3bzZXp5V1hZybIQGPrSMpxII8A4YXrFbS0rp3C3lWHKAtalcoq7xpQMXNu4BlqndNs08oic73SXMfxcQcrdLKwizih1golK4hxH6vOOwu5SitB3y-gHHTcdZ93WmA6N6UZjkD17U1yZAdw_8t0JBCY74Lefw-b_St3Xy_ud5CuR4p0D</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2025313088</pqid></control><display><type>article</type><title>GC/MS analysis and characterization of 2‐Hexadecen‐1‐ol and beta sitosterol from Schimpera arabica extract for its bioactive potential as antioxidant and antimicrobial</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Hidayathulla, S. ; Shahat, A.A. ; Ahamad, S.R. ; Al Moqbil, A.A.N. ; Alsaid, M.S. ; Divakar, D.D.</creator><creatorcontrib>Hidayathulla, S. ; Shahat, A.A. ; Ahamad, S.R. ; Al Moqbil, A.A.N. ; Alsaid, M.S. ; Divakar, D.D.</creatorcontrib><description>Aims
We evaluated the in vitro antioxidant and antimicrobial potential of Schimpera arabica extract and fractions (2‐Hexadecen‐1‐ol and beta sitosterol).
Methods and Results
Free radical scavenging activity was estimated by using ABTS and DPPH methods, reducing power and iron chelation was studied to investigate antioxidant potential of the extracts. Well‐diffusion method was utilized to evaluate the antimicrobial activity of the extracts. Gas chromatography‐mass spectroscopy analysis of the extract revealed the presence of 27 compounds. Phenolic and flavonoid contents were found in high amount (7·6–18 mg GAE, mg g−1; 3·2–10·8 mg catechin, mg g−1) in dry plant material.
Conclusions
Among all the extracts, 2‐Hexadecen‐1‐ol and beta sitosterol were the major constituents of crude extract and exhibited significant antioxidant potential as well antimicrobial activity; however, all other fractions also exhibited fare degree of antioxidant potential. 93% DPPH was scavenged by 100 μg ml−1 of the ethyl acetate fraction; in ABTS assay chelation was 86·2 and 88% in ferric chloride reducing power assay.
Significance and Impact of the Study
Schimpera arabica is naturally present and used in food preparation in most Arabian nations. The characterization of S. arabica would provide an insight into its medicinal use as home remedial for bacterial infection and as antioxidant. Significant and comparable antimicrobial activity was demonstrated by both 2‐Hexadecen‐1‐ol and beta sitosterol fractions. The results suggest that S. arabica contain a good amount of natural antioxidants of medicinal importance. However, further investigations are needed to characterize the further activity of 2‐Hexadecen‐1‐ol and beta sitosterol in vivo.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.13704</identifier><identifier>PMID: 29356238</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>2‐Hexadecen‐1‐ol ; ABTS ; Acetic acid ; Antiinfectives and antibacterials ; antimicrobial ; Antimicrobial activity ; Antimicrobial agents ; antioxidant ; Antioxidants ; beta sitosterol ; Catechin ; Chelation ; DPPH ; Ethyl acetate ; Ferric chloride ; Free radicals ; Gas chromatography ; GC/MS ; Iron chlorides ; Iron constituents ; Mass spectroscopy ; Phenolic compounds ; Phenols ; Schimpera arabica ; Sepia arabica ; total phenols</subject><ispartof>Journal of applied microbiology, 2018-05, Vol.124 (5), p.1082-1091</ispartof><rights>2018 The Society for Applied Microbiology</rights><rights>This article is protected by copyright. All rights reserved.</rights><rights>Copyright © 2018 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3534-2c35f4c070482ebd2bf71c635d75d795b07cec6347d71d8b40ed98d8e9d7a9883</citedby><cites>FETCH-LOGICAL-c3534-2c35f4c070482ebd2bf71c635d75d795b07cec6347d71d8b40ed98d8e9d7a9883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.13704$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.13704$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29356238$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hidayathulla, S.</creatorcontrib><creatorcontrib>Shahat, A.A.</creatorcontrib><creatorcontrib>Ahamad, S.R.</creatorcontrib><creatorcontrib>Al Moqbil, A.A.N.</creatorcontrib><creatorcontrib>Alsaid, M.S.</creatorcontrib><creatorcontrib>Divakar, D.D.</creatorcontrib><title>GC/MS analysis and characterization of 2‐Hexadecen‐1‐ol and beta sitosterol from Schimpera arabica extract for its bioactive potential as antioxidant and antimicrobial</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
We evaluated the in vitro antioxidant and antimicrobial potential of Schimpera arabica extract and fractions (2‐Hexadecen‐1‐ol and beta sitosterol).
Methods and Results
Free radical scavenging activity was estimated by using ABTS and DPPH methods, reducing power and iron chelation was studied to investigate antioxidant potential of the extracts. Well‐diffusion method was utilized to evaluate the antimicrobial activity of the extracts. Gas chromatography‐mass spectroscopy analysis of the extract revealed the presence of 27 compounds. Phenolic and flavonoid contents were found in high amount (7·6–18 mg GAE, mg g−1; 3·2–10·8 mg catechin, mg g−1) in dry plant material.
Conclusions
Among all the extracts, 2‐Hexadecen‐1‐ol and beta sitosterol were the major constituents of crude extract and exhibited significant antioxidant potential as well antimicrobial activity; however, all other fractions also exhibited fare degree of antioxidant potential. 93% DPPH was scavenged by 100 μg ml−1 of the ethyl acetate fraction; in ABTS assay chelation was 86·2 and 88% in ferric chloride reducing power assay.
Significance and Impact of the Study
Schimpera arabica is naturally present and used in food preparation in most Arabian nations. The characterization of S. arabica would provide an insight into its medicinal use as home remedial for bacterial infection and as antioxidant. Significant and comparable antimicrobial activity was demonstrated by both 2‐Hexadecen‐1‐ol and beta sitosterol fractions. The results suggest that S. arabica contain a good amount of natural antioxidants of medicinal importance. However, further investigations are needed to characterize the further activity of 2‐Hexadecen‐1‐ol and beta sitosterol in vivo.</description><subject>2‐Hexadecen‐1‐ol</subject><subject>ABTS</subject><subject>Acetic acid</subject><subject>Antiinfectives and antibacterials</subject><subject>antimicrobial</subject><subject>Antimicrobial activity</subject><subject>Antimicrobial agents</subject><subject>antioxidant</subject><subject>Antioxidants</subject><subject>beta sitosterol</subject><subject>Catechin</subject><subject>Chelation</subject><subject>DPPH</subject><subject>Ethyl acetate</subject><subject>Ferric chloride</subject><subject>Free radicals</subject><subject>Gas chromatography</subject><subject>GC/MS</subject><subject>Iron chlorides</subject><subject>Iron constituents</subject><subject>Mass spectroscopy</subject><subject>Phenolic compounds</subject><subject>Phenols</subject><subject>Schimpera arabica</subject><subject>Sepia arabica</subject><subject>total phenols</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kUtOHDEQhq0IFAiw4ALIEqssmvGju929RCMeiUBZkKxbflQLj6bHE9uTzGTFEbhILsVJqOmB7GLZqirr8-9S_YSccnbBcU1merjgUrHyAznksq4KUSuxN-ZlUTElDsinlGaMccmq-iM5EK2saiGbQ_L3Zjq5f6B6oeeb5BMmjtpHHbXNEP0fnX1Y0NBT8fL0fAtr7cDCAnOOJ8xH3EDWNPkcEj7Buz6GgT7YRz8sIWqKWsZbTWGdt6q0D5H6nKjxAUv_C-gyZFhkr1Fu2wB-ufYO46i-rQdvYzAIHJP9Xs8TnLzFI_Lj-ur79La4-3bzZXp5V1hZybIQGPrSMpxII8A4YXrFbS0rp3C3lWHKAtalcoq7xpQMXNu4BlqndNs08oic73SXMfxcQcrdLKwizih1golK4hxH6vOOwu5SitB3y-gHHTcdZ93WmA6N6UZjkD17U1yZAdw_8t0JBCY74Lefw-b_St3Xy_ud5CuR4p0D</recordid><startdate>201805</startdate><enddate>201805</enddate><creator>Hidayathulla, S.</creator><creator>Shahat, A.A.</creator><creator>Ahamad, S.R.</creator><creator>Al Moqbil, A.A.N.</creator><creator>Alsaid, M.S.</creator><creator>Divakar, D.D.</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201805</creationdate><title>GC/MS analysis and characterization of 2‐Hexadecen‐1‐ol and beta sitosterol from Schimpera arabica extract for its bioactive potential as antioxidant and antimicrobial</title><author>Hidayathulla, S. ; Shahat, A.A. ; Ahamad, S.R. ; Al Moqbil, A.A.N. ; Alsaid, M.S. ; Divakar, D.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3534-2c35f4c070482ebd2bf71c635d75d795b07cec6347d71d8b40ed98d8e9d7a9883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>2‐Hexadecen‐1‐ol</topic><topic>ABTS</topic><topic>Acetic acid</topic><topic>Antiinfectives and antibacterials</topic><topic>antimicrobial</topic><topic>Antimicrobial activity</topic><topic>Antimicrobial agents</topic><topic>antioxidant</topic><topic>Antioxidants</topic><topic>beta sitosterol</topic><topic>Catechin</topic><topic>Chelation</topic><topic>DPPH</topic><topic>Ethyl acetate</topic><topic>Ferric chloride</topic><topic>Free radicals</topic><topic>Gas chromatography</topic><topic>GC/MS</topic><topic>Iron chlorides</topic><topic>Iron constituents</topic><topic>Mass spectroscopy</topic><topic>Phenolic compounds</topic><topic>Phenols</topic><topic>Schimpera arabica</topic><topic>Sepia arabica</topic><topic>total phenols</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hidayathulla, S.</creatorcontrib><creatorcontrib>Shahat, A.A.</creatorcontrib><creatorcontrib>Ahamad, S.R.</creatorcontrib><creatorcontrib>Al Moqbil, A.A.N.</creatorcontrib><creatorcontrib>Alsaid, M.S.</creatorcontrib><creatorcontrib>Divakar, D.D.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hidayathulla, S.</au><au>Shahat, A.A.</au><au>Ahamad, S.R.</au><au>Al Moqbil, A.A.N.</au><au>Alsaid, M.S.</au><au>Divakar, D.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GC/MS analysis and characterization of 2‐Hexadecen‐1‐ol and beta sitosterol from Schimpera arabica extract for its bioactive potential as antioxidant and antimicrobial</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2018-05</date><risdate>2018</risdate><volume>124</volume><issue>5</issue><spage>1082</spage><epage>1091</epage><pages>1082-1091</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
We evaluated the in vitro antioxidant and antimicrobial potential of Schimpera arabica extract and fractions (2‐Hexadecen‐1‐ol and beta sitosterol).
Methods and Results
Free radical scavenging activity was estimated by using ABTS and DPPH methods, reducing power and iron chelation was studied to investigate antioxidant potential of the extracts. Well‐diffusion method was utilized to evaluate the antimicrobial activity of the extracts. Gas chromatography‐mass spectroscopy analysis of the extract revealed the presence of 27 compounds. Phenolic and flavonoid contents were found in high amount (7·6–18 mg GAE, mg g−1; 3·2–10·8 mg catechin, mg g−1) in dry plant material.
Conclusions
Among all the extracts, 2‐Hexadecen‐1‐ol and beta sitosterol were the major constituents of crude extract and exhibited significant antioxidant potential as well antimicrobial activity; however, all other fractions also exhibited fare degree of antioxidant potential. 93% DPPH was scavenged by 100 μg ml−1 of the ethyl acetate fraction; in ABTS assay chelation was 86·2 and 88% in ferric chloride reducing power assay.
Significance and Impact of the Study
Schimpera arabica is naturally present and used in food preparation in most Arabian nations. The characterization of S. arabica would provide an insight into its medicinal use as home remedial for bacterial infection and as antioxidant. Significant and comparable antimicrobial activity was demonstrated by both 2‐Hexadecen‐1‐ol and beta sitosterol fractions. The results suggest that S. arabica contain a good amount of natural antioxidants of medicinal importance. However, further investigations are needed to characterize the further activity of 2‐Hexadecen‐1‐ol and beta sitosterol in vivo.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>29356238</pmid><doi>10.1111/jam.13704</doi><tpages>10</tpages></addata></record> |
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| ispartof | Journal of applied microbiology, 2018-05, Vol.124 (5), p.1082-1091 |
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| source | Oxford University Press Journals All Titles (1996-Current); Wiley Online Library Journals Frontfile Complete |
| subjects | 2‐Hexadecen‐1‐ol ABTS Acetic acid Antiinfectives and antibacterials antimicrobial Antimicrobial activity Antimicrobial agents antioxidant Antioxidants beta sitosterol Catechin Chelation DPPH Ethyl acetate Ferric chloride Free radicals Gas chromatography GC/MS Iron chlorides Iron constituents Mass spectroscopy Phenolic compounds Phenols Schimpera arabica Sepia arabica total phenols |
| title | GC/MS analysis and characterization of 2‐Hexadecen‐1‐ol and beta sitosterol from Schimpera arabica extract for its bioactive potential as antioxidant and antimicrobial |
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