The cotton [Gossypium hirsutum] GhNHX1 gene encoding a novel putative tonoplast Na+/H+ antiporter plays and important role in salt stress
A cDNA clone was isolated from cotton (Gossypium hirsutum) cDNA library and characterized with regard to its sequence, regulation in response to salt stress and functions in yeast mutants and transgenic tobacco plants. The clone, designated as GhNHX1, contains 2,485 nucleotides with an open reading...
Gespeichert in:
| Veröffentlicht in: | Plant and cell physiology 2004-05, Vol.45 (5), p.600-607 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 607 |
|---|---|
| container_issue | 5 |
| container_start_page | 600 |
| container_title | Plant and cell physiology |
| container_volume | 45 |
| creator | Wu, C.A. (Shandong Agricultural Univ. (China)) Yang, G.D Meng, Q.W Zheng, C.C |
| description | A cDNA clone was isolated from cotton (Gossypium hirsutum) cDNA library and characterized with regard to its sequence, regulation in response to salt stress and functions in yeast mutants and transgenic tobacco plants. The clone, designated as GhNHX1, contains 2,485 nucleotides with an open reading frame of 1,629 nucleotides, and the deduced amino acid sequence showed high identities with other plant vacuolar-type Na+/H+ antiporters. Northern blot analysis indicated that the mRNA accumulation of GhNHX1 was strongly induced by salt stress and abscisic acid in cotton seedlings. The expression of GhNHX1 in yeast Na+/H+ antiporter mutant showed function complementation. The transgenic tobacco plants overexpressing GhNHX1 also had higher salt tolerance than the wild-type plants. The salt-induced mRNA level of GhNHX1 was 3 and 7 times higher in the salt-tolerant cotton cultivar ZM3 than those in the salt-sensitive cotton cultivars ZMS17 and ZMS12, respectively. Together, these results suggest that the products of the novel gene, GhNHX1, function as a tonoplast Na+/H+ antiporter and play an important role in salt tolerance of cotton. |
| doi_str_mv | 10.1093/pcp/pch071 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_201573200</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>649022001</sourcerecordid><originalsourceid>FETCH-LOGICAL-c485t-aa6f1f534b5de79658578eedda5d9134b738d85170dc597dcf0f9da1b24c49503</originalsourceid><addsrcrecordid>eNpdkFFv0zAUhS0EYmXwwjtg8cgUdh3HcfyIKmiZpoJEJ01DyHJjp_VI4mA7E_0J-9dzlWpIPFjXOve791wdhF4T-EhA0POhHtLbASdP0IwUnGQCGH2KZgA0z4BX5AS9COEWIP0pPEcnhJFSiCKfofv1zuDaxeh6_HPhQtgPduzwzvowxrH7hRe71fKa4K3pDTZ97bTtt1jh3t2ZFg9jVNHeGZzG3dCqEPFKnZ0vz7Dqox2cj8bjpO9DEjS23UFKLexda7DtcVBtxCF6E8JL9KxRbTCvjvUUXX35vJ4vs8tvi6_zT5dZXVQsZkqVDWkYLTZMGy5KVjFeGaO1YlqQJHNa6YoRDrpmguu6gUZoRTZ5UReCAT1F76e9g3d_RhOivHWj75OlzIEwTnM4QB8mqPYpE28aOXjbKb-XBOQhdJlCl1PoCX573DhuOqP_oceUE5BNgA3R_H3sK_9blpxyJpfXN3JFbkCs52s5T_y7iW-Uk2rrbZBXP9JxFJIzE_nB8s3_xMX3dDgDIEWZ0weoCqCc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>201573200</pqid></control><display><type>article</type><title>The cotton [Gossypium hirsutum] GhNHX1 gene encoding a novel putative tonoplast Na+/H+ antiporter plays and important role in salt stress</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Wu, C.A. (Shandong Agricultural Univ. (China)) ; Yang, G.D ; Meng, Q.W ; Zheng, C.C</creator><creatorcontrib>Wu, C.A. (Shandong Agricultural Univ. (China)) ; Yang, G.D ; Meng, Q.W ; Zheng, C.C</creatorcontrib><description>A cDNA clone was isolated from cotton (Gossypium hirsutum) cDNA library and characterized with regard to its sequence, regulation in response to salt stress and functions in yeast mutants and transgenic tobacco plants. The clone, designated as GhNHX1, contains 2,485 nucleotides with an open reading frame of 1,629 nucleotides, and the deduced amino acid sequence showed high identities with other plant vacuolar-type Na+/H+ antiporters. Northern blot analysis indicated that the mRNA accumulation of GhNHX1 was strongly induced by salt stress and abscisic acid in cotton seedlings. The expression of GhNHX1 in yeast Na+/H+ antiporter mutant showed function complementation. The transgenic tobacco plants overexpressing GhNHX1 also had higher salt tolerance than the wild-type plants. The salt-induced mRNA level of GhNHX1 was 3 and 7 times higher in the salt-tolerant cotton cultivar ZM3 than those in the salt-sensitive cotton cultivars ZMS17 and ZMS12, respectively. Together, these results suggest that the products of the novel gene, GhNHX1, function as a tonoplast Na+/H+ antiporter and play an important role in salt tolerance of cotton.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/pch071</identifier><identifier>PMID: 15169942</identifier><language>eng</language><publisher>Japan: Oxford University Press</publisher><subject>ABA ; abscisic acid ; Abscisic Acid - pharmacology ; Amino Acid Sequence - genetics ; amino acid sequences ; antiporters ; bakers yeast ; Base Sequence - genetics ; complementary DNA ; cotton ; cultivars ; DNA libraries ; DNA, Complementary - analysis ; DNA, Complementary - genetics ; fiber crops ; gene expression regulation ; gene overexpression ; genetic complementation ; Gossypium - drug effects ; Gossypium - genetics ; Gossypium - metabolism ; GOSSYPIUM HIRSUTUM ; Keywords: ABA — Cotton (Gossypium hirsutum) — cDNA library — Salt stress — Transgenic tobacco — Yeast Na+/H+ antiporter ; messenger RNA ; MOLECULAR CLONING ; Molecular Sequence Data ; Mutation - drug effects ; Mutation - genetics ; NICOTIANA TABACUM ; NUCLEOTIDE SEQUENCE ; OSMOTIC STRESS ; plant proteins ; Plant Proteins - genetics ; Plant Proteins - isolation & purification ; Plants, Genetically Modified - drug effects ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; RNA, Messenger - drug effects ; RNA, Messenger - metabolism ; Saccharomyces cerevisiae ; salicylic acid ; salt stress ; Seedlings - drug effects ; Seedlings - genetics ; Seedlings - metabolism ; sequence alignment ; Sequence Homology, Amino Acid ; Sodium Chloride - toxicity ; sodium-hydrogen antiporter ; Sodium-Hydrogen Exchangers - genetics ; Sodium-Hydrogen Exchangers - isolation & purification ; tobacco ; tonoplast ; TRANSGENIC PLANTS ; transmembrane</subject><ispartof>Plant and cell physiology, 2004-05, Vol.45 (5), p.600-607</ispartof><rights>Copyright Oxford University Press(England) May 15, 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-aa6f1f534b5de79658578eedda5d9134b738d85170dc597dcf0f9da1b24c49503</citedby><cites>FETCH-LOGICAL-c485t-aa6f1f534b5de79658578eedda5d9134b738d85170dc597dcf0f9da1b24c49503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15169942$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, C.A. (Shandong Agricultural Univ. (China))</creatorcontrib><creatorcontrib>Yang, G.D</creatorcontrib><creatorcontrib>Meng, Q.W</creatorcontrib><creatorcontrib>Zheng, C.C</creatorcontrib><title>The cotton [Gossypium hirsutum] GhNHX1 gene encoding a novel putative tonoplast Na+/H+ antiporter plays and important role in salt stress</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>A cDNA clone was isolated from cotton (Gossypium hirsutum) cDNA library and characterized with regard to its sequence, regulation in response to salt stress and functions in yeast mutants and transgenic tobacco plants. The clone, designated as GhNHX1, contains 2,485 nucleotides with an open reading frame of 1,629 nucleotides, and the deduced amino acid sequence showed high identities with other plant vacuolar-type Na+/H+ antiporters. Northern blot analysis indicated that the mRNA accumulation of GhNHX1 was strongly induced by salt stress and abscisic acid in cotton seedlings. The expression of GhNHX1 in yeast Na+/H+ antiporter mutant showed function complementation. The transgenic tobacco plants overexpressing GhNHX1 also had higher salt tolerance than the wild-type plants. The salt-induced mRNA level of GhNHX1 was 3 and 7 times higher in the salt-tolerant cotton cultivar ZM3 than those in the salt-sensitive cotton cultivars ZMS17 and ZMS12, respectively. Together, these results suggest that the products of the novel gene, GhNHX1, function as a tonoplast Na+/H+ antiporter and play an important role in salt tolerance of cotton.</description><subject>ABA</subject><subject>abscisic acid</subject><subject>Abscisic Acid - pharmacology</subject><subject>Amino Acid Sequence - genetics</subject><subject>amino acid sequences</subject><subject>antiporters</subject><subject>bakers yeast</subject><subject>Base Sequence - genetics</subject><subject>complementary DNA</subject><subject>cotton</subject><subject>cultivars</subject><subject>DNA libraries</subject><subject>DNA, Complementary - analysis</subject><subject>DNA, Complementary - genetics</subject><subject>fiber crops</subject><subject>gene expression regulation</subject><subject>gene overexpression</subject><subject>genetic complementation</subject><subject>Gossypium - drug effects</subject><subject>Gossypium - genetics</subject><subject>Gossypium - metabolism</subject><subject>GOSSYPIUM HIRSUTUM</subject><subject>Keywords: ABA — Cotton (Gossypium hirsutum) — cDNA library — Salt stress — Transgenic tobacco — Yeast Na+/H+ antiporter</subject><subject>messenger RNA</subject><subject>MOLECULAR CLONING</subject><subject>Molecular Sequence Data</subject><subject>Mutation - drug effects</subject><subject>Mutation - genetics</subject><subject>NICOTIANA TABACUM</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>OSMOTIC STRESS</subject><subject>plant proteins</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - isolation & purification</subject><subject>Plants, Genetically Modified - drug effects</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>RNA, Messenger - drug effects</subject><subject>RNA, Messenger - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>salicylic acid</subject><subject>salt stress</subject><subject>Seedlings - drug effects</subject><subject>Seedlings - genetics</subject><subject>Seedlings - metabolism</subject><subject>sequence alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sodium Chloride - toxicity</subject><subject>sodium-hydrogen antiporter</subject><subject>Sodium-Hydrogen Exchangers - genetics</subject><subject>Sodium-Hydrogen Exchangers - isolation & purification</subject><subject>tobacco</subject><subject>tonoplast</subject><subject>TRANSGENIC PLANTS</subject><subject>transmembrane</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkFFv0zAUhS0EYmXwwjtg8cgUdh3HcfyIKmiZpoJEJ01DyHJjp_VI4mA7E_0J-9dzlWpIPFjXOve791wdhF4T-EhA0POhHtLbASdP0IwUnGQCGH2KZgA0z4BX5AS9COEWIP0pPEcnhJFSiCKfofv1zuDaxeh6_HPhQtgPduzwzvowxrH7hRe71fKa4K3pDTZ97bTtt1jh3t2ZFg9jVNHeGZzG3dCqEPFKnZ0vz7Dqox2cj8bjpO9DEjS23UFKLexda7DtcVBtxCF6E8JL9KxRbTCvjvUUXX35vJ4vs8tvi6_zT5dZXVQsZkqVDWkYLTZMGy5KVjFeGaO1YlqQJHNa6YoRDrpmguu6gUZoRTZ5UReCAT1F76e9g3d_RhOivHWj75OlzIEwTnM4QB8mqPYpE28aOXjbKb-XBOQhdJlCl1PoCX573DhuOqP_oceUE5BNgA3R_H3sK_9blpxyJpfXN3JFbkCs52s5T_y7iW-Uk2rrbZBXP9JxFJIzE_nB8s3_xMX3dDgDIEWZ0weoCqCc</recordid><startdate>200405</startdate><enddate>200405</enddate><creator>Wu, C.A. (Shandong Agricultural Univ. (China))</creator><creator>Yang, G.D</creator><creator>Meng, Q.W</creator><creator>Zheng, C.C</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200405</creationdate><title>The cotton [Gossypium hirsutum] GhNHX1 gene encoding a novel putative tonoplast Na+/H+ antiporter plays and important role in salt stress</title><author>Wu, C.A. (Shandong Agricultural Univ. (China)) ; Yang, G.D ; Meng, Q.W ; Zheng, C.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-aa6f1f534b5de79658578eedda5d9134b738d85170dc597dcf0f9da1b24c49503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>ABA</topic><topic>abscisic acid</topic><topic>Abscisic Acid - pharmacology</topic><topic>Amino Acid Sequence - genetics</topic><topic>amino acid sequences</topic><topic>antiporters</topic><topic>bakers yeast</topic><topic>Base Sequence - genetics</topic><topic>complementary DNA</topic><topic>cotton</topic><topic>cultivars</topic><topic>DNA libraries</topic><topic>DNA, Complementary - analysis</topic><topic>DNA, Complementary - genetics</topic><topic>fiber crops</topic><topic>gene expression regulation</topic><topic>gene overexpression</topic><topic>genetic complementation</topic><topic>Gossypium - drug effects</topic><topic>Gossypium - genetics</topic><topic>Gossypium - metabolism</topic><topic>GOSSYPIUM HIRSUTUM</topic><topic>Keywords: ABA — Cotton (Gossypium hirsutum) — cDNA library — Salt stress — Transgenic tobacco — Yeast Na+/H+ antiporter</topic><topic>messenger RNA</topic><topic>MOLECULAR CLONING</topic><topic>Molecular Sequence Data</topic><topic>Mutation - drug effects</topic><topic>Mutation - genetics</topic><topic>NICOTIANA TABACUM</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>OSMOTIC STRESS</topic><topic>plant proteins</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - isolation & purification</topic><topic>Plants, Genetically Modified - drug effects</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>RNA, Messenger - drug effects</topic><topic>RNA, Messenger - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>salicylic acid</topic><topic>salt stress</topic><topic>Seedlings - drug effects</topic><topic>Seedlings - genetics</topic><topic>Seedlings - metabolism</topic><topic>sequence alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sodium Chloride - toxicity</topic><topic>sodium-hydrogen antiporter</topic><topic>Sodium-Hydrogen Exchangers - genetics</topic><topic>Sodium-Hydrogen Exchangers - isolation & purification</topic><topic>tobacco</topic><topic>tonoplast</topic><topic>TRANSGENIC PLANTS</topic><topic>transmembrane</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, C.A. (Shandong Agricultural Univ. (China))</creatorcontrib><creatorcontrib>Yang, G.D</creatorcontrib><creatorcontrib>Meng, Q.W</creatorcontrib><creatorcontrib>Zheng, C.C</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant and cell physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, C.A. (Shandong Agricultural Univ. (China))</au><au>Yang, G.D</au><au>Meng, Q.W</au><au>Zheng, C.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The cotton [Gossypium hirsutum] GhNHX1 gene encoding a novel putative tonoplast Na+/H+ antiporter plays and important role in salt stress</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2004-05</date><risdate>2004</risdate><volume>45</volume><issue>5</issue><spage>600</spage><epage>607</epage><pages>600-607</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>A cDNA clone was isolated from cotton (Gossypium hirsutum) cDNA library and characterized with regard to its sequence, regulation in response to salt stress and functions in yeast mutants and transgenic tobacco plants. The clone, designated as GhNHX1, contains 2,485 nucleotides with an open reading frame of 1,629 nucleotides, and the deduced amino acid sequence showed high identities with other plant vacuolar-type Na+/H+ antiporters. Northern blot analysis indicated that the mRNA accumulation of GhNHX1 was strongly induced by salt stress and abscisic acid in cotton seedlings. The expression of GhNHX1 in yeast Na+/H+ antiporter mutant showed function complementation. The transgenic tobacco plants overexpressing GhNHX1 also had higher salt tolerance than the wild-type plants. The salt-induced mRNA level of GhNHX1 was 3 and 7 times higher in the salt-tolerant cotton cultivar ZM3 than those in the salt-sensitive cotton cultivars ZMS17 and ZMS12, respectively. Together, these results suggest that the products of the novel gene, GhNHX1, function as a tonoplast Na+/H+ antiporter and play an important role in salt tolerance of cotton.</abstract><cop>Japan</cop><pub>Oxford University Press</pub><pmid>15169942</pmid><doi>10.1093/pcp/pch071</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0781 |
| ispartof | Plant and cell physiology, 2004-05, Vol.45 (5), p.600-607 |
| issn | 0032-0781 1471-9053 |
| language | eng |
| recordid | cdi_proquest_journals_201573200 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | ABA abscisic acid Abscisic Acid - pharmacology Amino Acid Sequence - genetics amino acid sequences antiporters bakers yeast Base Sequence - genetics complementary DNA cotton cultivars DNA libraries DNA, Complementary - analysis DNA, Complementary - genetics fiber crops gene expression regulation gene overexpression genetic complementation Gossypium - drug effects Gossypium - genetics Gossypium - metabolism GOSSYPIUM HIRSUTUM Keywords: ABA — Cotton (Gossypium hirsutum) — cDNA library — Salt stress — Transgenic tobacco — Yeast Na+/H+ antiporter messenger RNA MOLECULAR CLONING Molecular Sequence Data Mutation - drug effects Mutation - genetics NICOTIANA TABACUM NUCLEOTIDE SEQUENCE OSMOTIC STRESS plant proteins Plant Proteins - genetics Plant Proteins - isolation & purification Plants, Genetically Modified - drug effects Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism RNA, Messenger - drug effects RNA, Messenger - metabolism Saccharomyces cerevisiae salicylic acid salt stress Seedlings - drug effects Seedlings - genetics Seedlings - metabolism sequence alignment Sequence Homology, Amino Acid Sodium Chloride - toxicity sodium-hydrogen antiporter Sodium-Hydrogen Exchangers - genetics Sodium-Hydrogen Exchangers - isolation & purification tobacco tonoplast TRANSGENIC PLANTS transmembrane |
| title | The cotton [Gossypium hirsutum] GhNHX1 gene encoding a novel putative tonoplast Na+/H+ antiporter plays and important role in salt stress |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T11%3A06%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20cotton%20%5BGossypium%20hirsutum%5D%20GhNHX1%20gene%20encoding%20a%20novel%20putative%20tonoplast%20Na+/H+%20antiporter%20plays%20and%20important%20role%20in%20salt%20stress&rft.jtitle=Plant%20and%20cell%20physiology&rft.au=Wu,%20C.A.%20(Shandong%20Agricultural%20Univ.%20(China))&rft.date=2004-05&rft.volume=45&rft.issue=5&rft.spage=600&rft.epage=607&rft.pages=600-607&rft.issn=0032-0781&rft.eissn=1471-9053&rft_id=info:doi/10.1093/pcp/pch071&rft_dat=%3Cproquest_cross%3E649022001%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=201573200&rft_id=info:pmid/15169942&rfr_iscdi=true |