Morphological and molecular characterization of Diaporthe (anamorph Phomopsis) complex and pathogenicity of Diaporthe aspalathi isolates causing stem canker in soybean
The complex of Diaporthe (asexual morph) species occurring on soybean constitutes an important pathogenic group associated with diseases such as pod and stem blight, seed decay and stem canker. Stem canker, caused by Diaporthe aspalathi, has been reported as the most aggressive form of canker and it...
Gespeichert in:
| Veröffentlicht in: | European journal of plant pathology 2018-08, Vol.151 (4), p.1009-1025 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1025 |
|---|---|
| container_issue | 4 |
| container_start_page | 1009 |
| container_title | European journal of plant pathology |
| container_volume | 151 |
| creator | Brumer, Bruna B. Lopes-Caitar, Valéria S. Chicowski, Aline S. Beloti, Jessica D. Castanho, Fernanda M. Gregório da Silva, Danielle C. de Carvalho, Sandremir Lopes, Ivani O. N. Soares, Rafael M. Seixas, Claudine D. S. Abdelnoor, Ricardo V. Marcelino-Guimarães, Francismar C. |
| description | The complex of
Diaporthe
(asexual morph) species occurring on soybean constitutes an important pathogenic group associated with diseases such as pod and stem blight, seed decay and stem canker. Stem canker, caused by
Diaporthe aspalathi,
has been reported as the most aggressive form of canker and its occurrence has limited soybean crop productivity in the southern United States. The main form of pathogen control is the use of stem canker resistant soybean varieties. In this study, strains of
Diaporthe
and
Phomopsis
were isolated from stem and seeds of soybean in different locations in South America during the years 1989–2014. Genomic DNA from 26 isolates were analyzed by PCR-restriction fragment length polymorphism (RFLP) and Amplified Fragment Length Polymorphism (AFLP) techniques, and sequencing of internal transcribed spacer (ITS) regions of ribosomal DNA. The molecular analysis of ITS sequences by alignment with those of ex-type strains deposited in GenBank and morphological characteristics allowed the identification of
Phomopsis longicolla
,
D
.
phaseolorum
var.
sojae
,
D
.
caulivora
and
D
.
aspalathi
. An analysis of the pathogenicity of 13 isolates of D.
aspalathi
inoculated in soybean genotypes carrying different resistance genes to stem canker (
Rdm
1,
Rdm
2,
Rdm
3,
Rdm
4,
Rdm
5 and
Rdm
?) enabled us to identify the occurrence of at least three races of D.
aspalathi
occurring in Brazil. Among the isolates identified as
D. aspalathi
, both molecular and phenotypic analyses showed clustering depending on the date of collection and pathogenicity, which revealed the existence of variability of the pathogen. |
| doi_str_mv | 10.1007/s10658-018-1436-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2015678547</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2015678547</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-17a4165de6206442a6d5d6fbf4bb36fbe5d335326a67e74186a131076c52044c3</originalsourceid><addsrcrecordid>eNp1kc1O3DAUha0KpA5DH6A7S2zaRagd_yXLakoL0lSwoGvrjuNMPE3sYCcSwwv1NethkBALVude3fOduzgIfabkkhKiviVKpKgKQquCciYL8QEtqFCsqLisT9CC1GVd0Eqxj-gspR3JTF2XC_Tvd4hjF_qwdQZ6DL7BQ-itmXuI2HQQwUw2uieYXPA4tPiHgzHEqbP4C3gYDjS-68IQxuTSV2zCMPb28TlohKkLW-udcdP-LQtphD6fHXYp5MEmbGBOzm9xmuyQF__XRuw8TmG_seDP0WkLfbKfXnSJ_vy8ul9dF-vbXzer7-vCMCqngirgVIrGypJIzkuQjWhku2n5ZsOyWtEwJlgpQSqrOK0kUEaJkkaUhHPDlujimDvG8DDbNOldmKPPL3VJqJCqElxlFz26TAwpRdvqMboB4l5Tog996GMfOvehD31okZnyyKTs9VsbX5Pfh_4DaxORSA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2015678547</pqid></control><display><type>article</type><title>Morphological and molecular characterization of Diaporthe (anamorph Phomopsis) complex and pathogenicity of Diaporthe aspalathi isolates causing stem canker in soybean</title><source>SpringerLink Journals - AutoHoldings</source><creator>Brumer, Bruna B. ; Lopes-Caitar, Valéria S. ; Chicowski, Aline S. ; Beloti, Jessica D. ; Castanho, Fernanda M. ; Gregório da Silva, Danielle C. ; de Carvalho, Sandremir ; Lopes, Ivani O. N. ; Soares, Rafael M. ; Seixas, Claudine D. S. ; Abdelnoor, Ricardo V. ; Marcelino-Guimarães, Francismar C.</creator><creatorcontrib>Brumer, Bruna B. ; Lopes-Caitar, Valéria S. ; Chicowski, Aline S. ; Beloti, Jessica D. ; Castanho, Fernanda M. ; Gregório da Silva, Danielle C. ; de Carvalho, Sandremir ; Lopes, Ivani O. N. ; Soares, Rafael M. ; Seixas, Claudine D. S. ; Abdelnoor, Ricardo V. ; Marcelino-Guimarães, Francismar C.</creatorcontrib><description>The complex of
Diaporthe
(asexual morph) species occurring on soybean constitutes an important pathogenic group associated with diseases such as pod and stem blight, seed decay and stem canker. Stem canker, caused by
Diaporthe aspalathi,
has been reported as the most aggressive form of canker and its occurrence has limited soybean crop productivity in the southern United States. The main form of pathogen control is the use of stem canker resistant soybean varieties. In this study, strains of
Diaporthe
and
Phomopsis
were isolated from stem and seeds of soybean in different locations in South America during the years 1989–2014. Genomic DNA from 26 isolates were analyzed by PCR-restriction fragment length polymorphism (RFLP) and Amplified Fragment Length Polymorphism (AFLP) techniques, and sequencing of internal transcribed spacer (ITS) regions of ribosomal DNA. The molecular analysis of ITS sequences by alignment with those of ex-type strains deposited in GenBank and morphological characteristics allowed the identification of
Phomopsis longicolla
,
D
.
phaseolorum
var.
sojae
,
D
.
caulivora
and
D
.
aspalathi
. An analysis of the pathogenicity of 13 isolates of D.
aspalathi
inoculated in soybean genotypes carrying different resistance genes to stem canker (
Rdm
1,
Rdm
2,
Rdm
3,
Rdm
4,
Rdm
5 and
Rdm
?) enabled us to identify the occurrence of at least three races of D.
aspalathi
occurring in Brazil. Among the isolates identified as
D. aspalathi
, both molecular and phenotypic analyses showed clustering depending on the date of collection and pathogenicity, which revealed the existence of variability of the pathogen.</description><identifier>ISSN: 0929-1873</identifier><identifier>EISSN: 1573-8469</identifier><identifier>DOI: 10.1007/s10658-018-1436-5</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agriculture ; Amplified fragment length polymorphism ; Biomedical and Life Sciences ; Blight ; Clustering ; Crop production ; Deoxyribonucleic acid ; Diaporthe ; DNA ; DNA sequencing ; Ecology ; Gene polymorphism ; Genotypes ; Glycine max ; Imperfect state ; Life Sciences ; Morphology ; Nucleotide sequence ; Pathogenicity ; Pathogens ; Physical characteristics ; Plant Pathology ; Plant Sciences ; Polymorphism ; Restriction fragment length polymorphism ; Ribosomal DNA ; Seeds ; Soybeans ; Spacer ; Stem blight ; Stem canker ; Strains (organisms)</subject><ispartof>European journal of plant pathology, 2018-08, Vol.151 (4), p.1009-1025</ispartof><rights>Koninklijke Nederlandse Planteziektenkundige Vereniging 2018</rights><rights>European Journal of Plant Pathology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-17a4165de6206442a6d5d6fbf4bb36fbe5d335326a67e74186a131076c52044c3</citedby><cites>FETCH-LOGICAL-c316t-17a4165de6206442a6d5d6fbf4bb36fbe5d335326a67e74186a131076c52044c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10658-018-1436-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10658-018-1436-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Brumer, Bruna B.</creatorcontrib><creatorcontrib>Lopes-Caitar, Valéria S.</creatorcontrib><creatorcontrib>Chicowski, Aline S.</creatorcontrib><creatorcontrib>Beloti, Jessica D.</creatorcontrib><creatorcontrib>Castanho, Fernanda M.</creatorcontrib><creatorcontrib>Gregório da Silva, Danielle C.</creatorcontrib><creatorcontrib>de Carvalho, Sandremir</creatorcontrib><creatorcontrib>Lopes, Ivani O. N.</creatorcontrib><creatorcontrib>Soares, Rafael M.</creatorcontrib><creatorcontrib>Seixas, Claudine D. S.</creatorcontrib><creatorcontrib>Abdelnoor, Ricardo V.</creatorcontrib><creatorcontrib>Marcelino-Guimarães, Francismar C.</creatorcontrib><title>Morphological and molecular characterization of Diaporthe (anamorph Phomopsis) complex and pathogenicity of Diaporthe aspalathi isolates causing stem canker in soybean</title><title>European journal of plant pathology</title><addtitle>Eur J Plant Pathol</addtitle><description>The complex of
Diaporthe
(asexual morph) species occurring on soybean constitutes an important pathogenic group associated with diseases such as pod and stem blight, seed decay and stem canker. Stem canker, caused by
Diaporthe aspalathi,
has been reported as the most aggressive form of canker and its occurrence has limited soybean crop productivity in the southern United States. The main form of pathogen control is the use of stem canker resistant soybean varieties. In this study, strains of
Diaporthe
and
Phomopsis
were isolated from stem and seeds of soybean in different locations in South America during the years 1989–2014. Genomic DNA from 26 isolates were analyzed by PCR-restriction fragment length polymorphism (RFLP) and Amplified Fragment Length Polymorphism (AFLP) techniques, and sequencing of internal transcribed spacer (ITS) regions of ribosomal DNA. The molecular analysis of ITS sequences by alignment with those of ex-type strains deposited in GenBank and morphological characteristics allowed the identification of
Phomopsis longicolla
,
D
.
phaseolorum
var.
sojae
,
D
.
caulivora
and
D
.
aspalathi
. An analysis of the pathogenicity of 13 isolates of D.
aspalathi
inoculated in soybean genotypes carrying different resistance genes to stem canker (
Rdm
1,
Rdm
2,
Rdm
3,
Rdm
4,
Rdm
5 and
Rdm
?) enabled us to identify the occurrence of at least three races of D.
aspalathi
occurring in Brazil. Among the isolates identified as
D. aspalathi
, both molecular and phenotypic analyses showed clustering depending on the date of collection and pathogenicity, which revealed the existence of variability of the pathogen.</description><subject>Agriculture</subject><subject>Amplified fragment length polymorphism</subject><subject>Biomedical and Life Sciences</subject><subject>Blight</subject><subject>Clustering</subject><subject>Crop production</subject><subject>Deoxyribonucleic acid</subject><subject>Diaporthe</subject><subject>DNA</subject><subject>DNA sequencing</subject><subject>Ecology</subject><subject>Gene polymorphism</subject><subject>Genotypes</subject><subject>Glycine max</subject><subject>Imperfect state</subject><subject>Life Sciences</subject><subject>Morphology</subject><subject>Nucleotide sequence</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Physical characteristics</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Polymorphism</subject><subject>Restriction fragment length polymorphism</subject><subject>Ribosomal DNA</subject><subject>Seeds</subject><subject>Soybeans</subject><subject>Spacer</subject><subject>Stem blight</subject><subject>Stem canker</subject><subject>Strains (organisms)</subject><issn>0929-1873</issn><issn>1573-8469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kc1O3DAUha0KpA5DH6A7S2zaRagd_yXLakoL0lSwoGvrjuNMPE3sYCcSwwv1NethkBALVude3fOduzgIfabkkhKiviVKpKgKQquCciYL8QEtqFCsqLisT9CC1GVd0Eqxj-gspR3JTF2XC_Tvd4hjF_qwdQZ6DL7BQ-itmXuI2HQQwUw2uieYXPA4tPiHgzHEqbP4C3gYDjS-68IQxuTSV2zCMPb28TlohKkLW-udcdP-LQtphD6fHXYp5MEmbGBOzm9xmuyQF__XRuw8TmG_seDP0WkLfbKfXnSJ_vy8ul9dF-vbXzer7-vCMCqngirgVIrGypJIzkuQjWhku2n5ZsOyWtEwJlgpQSqrOK0kUEaJkkaUhHPDlujimDvG8DDbNOldmKPPL3VJqJCqElxlFz26TAwpRdvqMboB4l5Tog996GMfOvehD31okZnyyKTs9VsbX5Pfh_4DaxORSA</recordid><startdate>20180801</startdate><enddate>20180801</enddate><creator>Brumer, Bruna B.</creator><creator>Lopes-Caitar, Valéria S.</creator><creator>Chicowski, Aline S.</creator><creator>Beloti, Jessica D.</creator><creator>Castanho, Fernanda M.</creator><creator>Gregório da Silva, Danielle C.</creator><creator>de Carvalho, Sandremir</creator><creator>Lopes, Ivani O. N.</creator><creator>Soares, Rafael M.</creator><creator>Seixas, Claudine D. S.</creator><creator>Abdelnoor, Ricardo V.</creator><creator>Marcelino-Guimarães, Francismar C.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X2</scope><scope>88A</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20180801</creationdate><title>Morphological and molecular characterization of Diaporthe (anamorph Phomopsis) complex and pathogenicity of Diaporthe aspalathi isolates causing stem canker in soybean</title><author>Brumer, Bruna B. ; Lopes-Caitar, Valéria S. ; Chicowski, Aline S. ; Beloti, Jessica D. ; Castanho, Fernanda M. ; Gregório da Silva, Danielle C. ; de Carvalho, Sandremir ; Lopes, Ivani O. N. ; Soares, Rafael M. ; Seixas, Claudine D. S. ; Abdelnoor, Ricardo V. ; Marcelino-Guimarães, Francismar C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-17a4165de6206442a6d5d6fbf4bb36fbe5d335326a67e74186a131076c52044c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Agriculture</topic><topic>Amplified fragment length polymorphism</topic><topic>Biomedical and Life Sciences</topic><topic>Blight</topic><topic>Clustering</topic><topic>Crop production</topic><topic>Deoxyribonucleic acid</topic><topic>Diaporthe</topic><topic>DNA</topic><topic>DNA sequencing</topic><topic>Ecology</topic><topic>Gene polymorphism</topic><topic>Genotypes</topic><topic>Glycine max</topic><topic>Imperfect state</topic><topic>Life Sciences</topic><topic>Morphology</topic><topic>Nucleotide sequence</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Physical characteristics</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Polymorphism</topic><topic>Restriction fragment length polymorphism</topic><topic>Ribosomal DNA</topic><topic>Seeds</topic><topic>Soybeans</topic><topic>Spacer</topic><topic>Stem blight</topic><topic>Stem canker</topic><topic>Strains (organisms)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brumer, Bruna B.</creatorcontrib><creatorcontrib>Lopes-Caitar, Valéria S.</creatorcontrib><creatorcontrib>Chicowski, Aline S.</creatorcontrib><creatorcontrib>Beloti, Jessica D.</creatorcontrib><creatorcontrib>Castanho, Fernanda M.</creatorcontrib><creatorcontrib>Gregório da Silva, Danielle C.</creatorcontrib><creatorcontrib>de Carvalho, Sandremir</creatorcontrib><creatorcontrib>Lopes, Ivani O. N.</creatorcontrib><creatorcontrib>Soares, Rafael M.</creatorcontrib><creatorcontrib>Seixas, Claudine D. S.</creatorcontrib><creatorcontrib>Abdelnoor, Ricardo V.</creatorcontrib><creatorcontrib>Marcelino-Guimarães, Francismar C.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>European journal of plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brumer, Bruna B.</au><au>Lopes-Caitar, Valéria S.</au><au>Chicowski, Aline S.</au><au>Beloti, Jessica D.</au><au>Castanho, Fernanda M.</au><au>Gregório da Silva, Danielle C.</au><au>de Carvalho, Sandremir</au><au>Lopes, Ivani O. N.</au><au>Soares, Rafael M.</au><au>Seixas, Claudine D. S.</au><au>Abdelnoor, Ricardo V.</au><au>Marcelino-Guimarães, Francismar C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphological and molecular characterization of Diaporthe (anamorph Phomopsis) complex and pathogenicity of Diaporthe aspalathi isolates causing stem canker in soybean</atitle><jtitle>European journal of plant pathology</jtitle><stitle>Eur J Plant Pathol</stitle><date>2018-08-01</date><risdate>2018</risdate><volume>151</volume><issue>4</issue><spage>1009</spage><epage>1025</epage><pages>1009-1025</pages><issn>0929-1873</issn><eissn>1573-8469</eissn><abstract>The complex of
Diaporthe
(asexual morph) species occurring on soybean constitutes an important pathogenic group associated with diseases such as pod and stem blight, seed decay and stem canker. Stem canker, caused by
Diaporthe aspalathi,
has been reported as the most aggressive form of canker and its occurrence has limited soybean crop productivity in the southern United States. The main form of pathogen control is the use of stem canker resistant soybean varieties. In this study, strains of
Diaporthe
and
Phomopsis
were isolated from stem and seeds of soybean in different locations in South America during the years 1989–2014. Genomic DNA from 26 isolates were analyzed by PCR-restriction fragment length polymorphism (RFLP) and Amplified Fragment Length Polymorphism (AFLP) techniques, and sequencing of internal transcribed spacer (ITS) regions of ribosomal DNA. The molecular analysis of ITS sequences by alignment with those of ex-type strains deposited in GenBank and morphological characteristics allowed the identification of
Phomopsis longicolla
,
D
.
phaseolorum
var.
sojae
,
D
.
caulivora
and
D
.
aspalathi
. An analysis of the pathogenicity of 13 isolates of D.
aspalathi
inoculated in soybean genotypes carrying different resistance genes to stem canker (
Rdm
1,
Rdm
2,
Rdm
3,
Rdm
4,
Rdm
5 and
Rdm
?) enabled us to identify the occurrence of at least three races of D.
aspalathi
occurring in Brazil. Among the isolates identified as
D. aspalathi
, both molecular and phenotypic analyses showed clustering depending on the date of collection and pathogenicity, which revealed the existence of variability of the pathogen.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10658-018-1436-5</doi><tpages>17</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0929-1873 |
| ispartof | European journal of plant pathology, 2018-08, Vol.151 (4), p.1009-1025 |
| issn | 0929-1873 1573-8469 |
| language | eng |
| recordid | cdi_proquest_journals_2015678547 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Agriculture Amplified fragment length polymorphism Biomedical and Life Sciences Blight Clustering Crop production Deoxyribonucleic acid Diaporthe DNA DNA sequencing Ecology Gene polymorphism Genotypes Glycine max Imperfect state Life Sciences Morphology Nucleotide sequence Pathogenicity Pathogens Physical characteristics Plant Pathology Plant Sciences Polymorphism Restriction fragment length polymorphism Ribosomal DNA Seeds Soybeans Spacer Stem blight Stem canker Strains (organisms) |
| title | Morphological and molecular characterization of Diaporthe (anamorph Phomopsis) complex and pathogenicity of Diaporthe aspalathi isolates causing stem canker in soybean |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T08%3A22%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Morphological%20and%20molecular%20characterization%20of%20Diaporthe%20(anamorph%20Phomopsis)%20complex%20and%20pathogenicity%20of%20Diaporthe%20aspalathi%20isolates%20causing%20stem%20canker%20in%20soybean&rft.jtitle=European%20journal%20of%20plant%20pathology&rft.au=Brumer,%20Bruna%20B.&rft.date=2018-08-01&rft.volume=151&rft.issue=4&rft.spage=1009&rft.epage=1025&rft.pages=1009-1025&rft.issn=0929-1873&rft.eissn=1573-8469&rft_id=info:doi/10.1007/s10658-018-1436-5&rft_dat=%3Cproquest_cross%3E2015678547%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2015678547&rft_id=info:pmid/&rfr_iscdi=true |