The 14-3-3 protein GF14c acts as a negative regulator of flowering in rice by interacting with the florigen Hd3a
Hd3a and FT proteins have recently been proposed to act as florigens in rice and Arabidopsis, respectively; however, the molecular mechanisms of their function remain to be determined. In this study, we identified GF14c (a 14-3-3 protein) as an Hd3a-interacting protein in a yeast two-hybrid screen....
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| Veröffentlicht in: | Plant and cell physiology 2009-03, Vol.50 (3), p.429-438 |
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| creator | Purwestri, Y.A.(Nara Inst. of Science and Technology, Ikoma (Japan)) Ogaki, Y Tamaki, S Tsuji, H Shimamoto, K |
| description | Hd3a and FT proteins have recently been proposed to act as florigens in rice and Arabidopsis, respectively; however, the molecular mechanisms of their function remain to be determined. In this study, we identified GF14c (a 14-3-3 protein) as an Hd3a-interacting protein in a yeast two-hybrid screen. In vitro and in vivo experiments, using a combination of pull-down assays and bimolecular fluorescence complementation, confirmed the interaction between Hd3a and GF14c. Functional analysis using either GF14c overexpression or knockout transgenic rice plants indicated that this interaction plays a role in the regulation of flowering. GF14c-overexpressing plants exhibited a delay in flowering and the knockout mutants displayed early flowering relative to the wild-type plants under short-day conditions. These results suggest that GF14c acts as a negative regulator of flowering by interacting with Hd3a. Since the 14-3-3 protein has been shown to interact with FT protein in tomato and Arabidopsis, our results in rice provide important findings about FT signaling in plants. |
| doi_str_mv | 10.1093/pcp/pcp012 |
| format | Article |
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In this study, we identified GF14c (a 14-3-3 protein) as an Hd3a-interacting protein in a yeast two-hybrid screen. In vitro and in vivo experiments, using a combination of pull-down assays and bimolecular fluorescence complementation, confirmed the interaction between Hd3a and GF14c. Functional analysis using either GF14c overexpression or knockout transgenic rice plants indicated that this interaction plays a role in the regulation of flowering. GF14c-overexpressing plants exhibited a delay in flowering and the knockout mutants displayed early flowering relative to the wild-type plants under short-day conditions. These results suggest that GF14c acts as a negative regulator of flowering by interacting with Hd3a. Since the 14-3-3 protein has been shown to interact with FT protein in tomato and Arabidopsis, our results in rice provide important findings about FT signaling in plants.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/pcp012</identifier><identifier>PMID: 19179350</identifier><language>eng</language><publisher>Japan: Oxford University Press</publisher><subject>14-3-3 Proteins - genetics ; 14-3-3 Proteins - metabolism ; DNA, Bacterial - metabolism ; DNA, Plant - genetics ; FLORACION ; FLORAISON ; FLOWERING ; Flowers - genetics ; Flowers - metabolism ; Gene Expression Regulation, Plant ; Gene Knockout Techniques ; Gene Library ; GF14c ; Hd3a ; IDENTIFICACION ; IDENTIFICATION ; LEVADURA ; LEVURE ; Mutagenesis, Insertional ; MUTANT ; MUTANTES ; MUTANTS ; Oryza - genetics ; Oryza - metabolism ; ORYZA SATIVA ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Protein interaction ; PROTEINAS ; PROTEINE ; PROTEINS ; Rice ; Two-Hybrid System Techniques ; YEASTS</subject><ispartof>Plant and cell physiology, 2009-03, Vol.50 (3), p.429-438</ispartof><rights>The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org 2009</rights><rights>The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. 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In this study, we identified GF14c (a 14-3-3 protein) as an Hd3a-interacting protein in a yeast two-hybrid screen. In vitro and in vivo experiments, using a combination of pull-down assays and bimolecular fluorescence complementation, confirmed the interaction between Hd3a and GF14c. Functional analysis using either GF14c overexpression or knockout transgenic rice plants indicated that this interaction plays a role in the regulation of flowering. GF14c-overexpressing plants exhibited a delay in flowering and the knockout mutants displayed early flowering relative to the wild-type plants under short-day conditions. These results suggest that GF14c acts as a negative regulator of flowering by interacting with Hd3a. Since the 14-3-3 protein has been shown to interact with FT protein in tomato and Arabidopsis, our results in rice provide important findings about FT signaling in plants.</description><subject>14-3-3 Proteins - genetics</subject><subject>14-3-3 Proteins - metabolism</subject><subject>DNA, Bacterial - metabolism</subject><subject>DNA, Plant - genetics</subject><subject>FLORACION</subject><subject>FLORAISON</subject><subject>FLOWERING</subject><subject>Flowers - genetics</subject><subject>Flowers - metabolism</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Knockout Techniques</subject><subject>Gene Library</subject><subject>GF14c</subject><subject>Hd3a</subject><subject>IDENTIFICACION</subject><subject>IDENTIFICATION</subject><subject>LEVADURA</subject><subject>LEVURE</subject><subject>Mutagenesis, Insertional</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>MUTANTS</subject><subject>Oryza - genetics</subject><subject>Oryza - metabolism</subject><subject>ORYZA SATIVA</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Protein interaction</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>PROTEINS</subject><subject>Rice</subject><subject>Two-Hybrid System Techniques</subject><subject>YEASTS</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kdFrFDEQxoMo9qy--K4EwRdhdbJJNpdHLV5PW7BgRfElZLOTbep1d02y1v735thD34QJ8xF-8w35QshTBq8ZaP5mctP-AKvvkRUTilUaJL9PVgC8rkCt2RF5lNI1QNEcHpIjppnSXMKKTJdXSJmoeMXpFMeMYaCnGyYctS4nakvRAXubwy-kEft5Z_MY6eip3423GMPQ0zISg0Pa3hWZMZbJ_fVtyFc0F_tCxtDjQLcdt4_JA293CZ8c-jH5snl_ebKtzj-dfjh5e145wde54lK0qNs1uKYGoXXb-sZ3DUom0EuUXNjag-5E51WNTmCrVSPWAgAdKPT8mLxYfMurfs6Ysrke5ziUlaYGJhulRF2gVwvk4phSRG-mGG5svDMMzD5bU3I1S7YFfn5wnNsb7P6hhzAL8HIBxnn6v1G1cCFl_P2XtPGHaRRX0my_fTcXX-XZ5vM7bc4K_2zhvR2N7WNI5uNFDaDL_2rB-B_eqpi7</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>Purwestri, Y.A.(Nara Inst. of Science and Technology, Ikoma (Japan))</creator><creator>Ogaki, Y</creator><creator>Tamaki, S</creator><creator>Tsuji, H</creator><creator>Shimamoto, K</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20090301</creationdate><title>The 14-3-3 protein GF14c acts as a negative regulator of flowering in rice by interacting with the florigen Hd3a</title><author>Purwestri, Y.A.(Nara Inst. of Science and Technology, Ikoma (Japan)) ; Ogaki, Y ; Tamaki, S ; Tsuji, H ; Shimamoto, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-354be9b80c620499bbf6fd6e514ef5e534a2f09d4df72ec4eb97648400ec07ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>14-3-3 Proteins - genetics</topic><topic>14-3-3 Proteins - metabolism</topic><topic>DNA, Bacterial - metabolism</topic><topic>DNA, Plant - genetics</topic><topic>FLORACION</topic><topic>FLORAISON</topic><topic>FLOWERING</topic><topic>Flowers - genetics</topic><topic>Flowers - metabolism</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Knockout Techniques</topic><topic>Gene Library</topic><topic>GF14c</topic><topic>Hd3a</topic><topic>IDENTIFICACION</topic><topic>IDENTIFICATION</topic><topic>LEVADURA</topic><topic>LEVURE</topic><topic>Mutagenesis, Insertional</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>MUTANTS</topic><topic>Oryza - genetics</topic><topic>Oryza - metabolism</topic><topic>ORYZA SATIVA</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Protein interaction</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>PROTEINS</topic><topic>Rice</topic><topic>Two-Hybrid System Techniques</topic><topic>YEASTS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Purwestri, Y.A.(Nara Inst. of Science and Technology, Ikoma (Japan))</creatorcontrib><creatorcontrib>Ogaki, Y</creatorcontrib><creatorcontrib>Tamaki, S</creatorcontrib><creatorcontrib>Tsuji, H</creatorcontrib><creatorcontrib>Shimamoto, K</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant and cell physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Purwestri, Y.A.(Nara Inst. of Science and Technology, Ikoma (Japan))</au><au>Ogaki, Y</au><au>Tamaki, S</au><au>Tsuji, H</au><au>Shimamoto, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The 14-3-3 protein GF14c acts as a negative regulator of flowering in rice by interacting with the florigen Hd3a</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2009-03-01</date><risdate>2009</risdate><volume>50</volume><issue>3</issue><spage>429</spage><epage>438</epage><pages>429-438</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>Hd3a and FT proteins have recently been proposed to act as florigens in rice and Arabidopsis, respectively; however, the molecular mechanisms of their function remain to be determined. In this study, we identified GF14c (a 14-3-3 protein) as an Hd3a-interacting protein in a yeast two-hybrid screen. In vitro and in vivo experiments, using a combination of pull-down assays and bimolecular fluorescence complementation, confirmed the interaction between Hd3a and GF14c. Functional analysis using either GF14c overexpression or knockout transgenic rice plants indicated that this interaction plays a role in the regulation of flowering. GF14c-overexpressing plants exhibited a delay in flowering and the knockout mutants displayed early flowering relative to the wild-type plants under short-day conditions. These results suggest that GF14c acts as a negative regulator of flowering by interacting with Hd3a. Since the 14-3-3 protein has been shown to interact with FT protein in tomato and Arabidopsis, our results in rice provide important findings about FT signaling in plants.</abstract><cop>Japan</cop><pub>Oxford University Press</pub><pmid>19179350</pmid><doi>10.1093/pcp/pcp012</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant and cell physiology, 2009-03, Vol.50 (3), p.429-438 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | 14-3-3 Proteins - genetics 14-3-3 Proteins - metabolism DNA, Bacterial - metabolism DNA, Plant - genetics FLORACION FLORAISON FLOWERING Flowers - genetics Flowers - metabolism Gene Expression Regulation, Plant Gene Knockout Techniques Gene Library GF14c Hd3a IDENTIFICACION IDENTIFICATION LEVADURA LEVURE Mutagenesis, Insertional MUTANT MUTANTES MUTANTS Oryza - genetics Oryza - metabolism ORYZA SATIVA Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Protein interaction PROTEINAS PROTEINE PROTEINS Rice Two-Hybrid System Techniques YEASTS |
| title | The 14-3-3 protein GF14c acts as a negative regulator of flowering in rice by interacting with the florigen Hd3a |
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