Functional Analysis of Water Channels in Barley Roots

We identified three genes homologous to water channels in the plasma membrane type subfamily from roots of barley seedlings. These genes were designated HvPIP2;1, HvPIP1;3, and HvPIP1;5 after comparison to Arabidopsis aquaporins. Competitive reverse transcription (RT)-PCR was applied in order to dis...

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Veröffentlicht in:	Plant and cell physiology 2002-08, Vol.43 (8), p.885-893
Hauptverfasser:	Katsuhara, Maki, Akiyama, Yoshiko, Koshio, Kazuki, Shibasaka, Mineo, Kasamo, Kunihiro
Format:	Artikel
Sprache:	eng
Schlagworte:	3-isobutyle-1-methylxanthine Adaptation, Physiological - genetics Adaptation, Physiological - physiology Algorithms Amino Acid Sequence Animals Aquaporins - antagonists & inhibitors Aquaporins - genetics Aquaporins - physiology Arabidopsis Proteins Carbazoles - pharmacology Cell Membrane - metabolism Cell Membrane Permeability - drug effects dimethylsulfoxide dithiothreitol DMSO DNA, Complementary - genetics DNA, Complementary - metabolism DTT Female Gene Expression Regulation, Plant Hordeum - drug effects Hordeum - genetics Hordeum - physiology IBMX Indole Alkaloids Ion Channels - genetics Keywords: Aquaporin — Barley — Competitive RT-PCR — Salt stress MBS Mercuric Chloride - pharmacology modified Barth’s solution Molecular Sequence Data N′-bis(2-ethanesulfonic acid) Okadaic Acid - pharmacology Oocytes - physiology Osmotic Pressure - drug effects Osmotic water permeability phenylmethylsulfonylfluoride Phosphoprotein Phosphatases - antagonists & inhibitors Phosphorylation Phylogeny PIP piperazine-N PIPES Plant Proteins - genetics Plant Proteins - metabolism Plant Roots - drug effects Plant Roots - genetics Plant Roots - physiology Plasma membrane Intrinsic Protein PMSF Protein Kinase Inhibitors RACE rapid amplification of cDNA end reverse transcription-PCR RT-PCR Sequence Homology, Amino Acid Sodium Chloride - pharmacology TIP Tonoplast Intrinsic Protein Water - pharmacology Water - physiology Xenopus laevis
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