Functional Analysis of Water Channels in Barley Roots
We identified three genes homologous to water channels in the plasma membrane type subfamily from roots of barley seedlings. These genes were designated HvPIP2;1, HvPIP1;3, and HvPIP1;5 after comparison to Arabidopsis aquaporins. Competitive reverse transcription (RT)-PCR was applied in order to dis...
Gespeichert in:
| Veröffentlicht in: | Plant and cell physiology 2002-08, Vol.43 (8), p.885-893 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 893 |
|---|---|
| container_issue | 8 |
| container_start_page | 885 |
| container_title | Plant and cell physiology |
| container_volume | 43 |
| creator | Katsuhara, Maki Akiyama, Yoshiko Koshio, Kazuki Shibasaka, Mineo Kasamo, Kunihiro |
| description | We identified three genes homologous to water channels in the plasma membrane type subfamily from roots of barley seedlings. These genes were designated HvPIP2;1, HvPIP1;3, and HvPIP1;5 after comparison to Arabidopsis aquaporins. Competitive reverse transcription (RT)-PCR was applied in order to distinguish and to quantify their transcripts. The HvPIP2;1 transcript was the most abundant among the three in roots. Salt stress (200 mM NaCl) down-regulated HvPIP2;1 (transcript and protein), but had almost no effect on the expressions of HvPIP1;3, or HvPIP1;5. Approximately equal amounts of the transcripts of the three were detected in shoots, and salt stress enhanced the expression of HvPIP2;1 but not of HvPIP1;3, or HvPIP1;5. HvPIP2;1 protein was confirmed to be localized in the plasma membrane. Functional expression of HvPIP2;1 in Xenopus oocytes confirmed that HvPIP2;1 encoded an aquaporin that transports water. This water permeability was reduced by HgCl2, which is a typical water channel inhibitor. This activity was not modified by some inhibitors against protein kinase and protein phosphatase. |
| doi_str_mv | 10.1093/pcp/pcf102 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_201533151</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>325279751</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-2d9154bf21ee0bf2a9ec4716f8b2c4e93342f90168501bc9a184fededee03c493</originalsourceid><addsrcrecordid>eNpFkNFLwzAQxoMobk5f_AOk-ChU75J0bR5ncc4xUUSZ7CWkXYKdXVuTFtx_b6RDOe6-g_vxcXyEnCNcIwh20-SNb4NAD8gQeYyhgIgdkiEAoyHECQ7IiXMbAL8zOCYDpCgSFDgk0bSr8raoK1UGEz92rnBBbYKlarUN0g9VVbp0QVEFt8qWehe81HXrTsmRUaXTZ3sdkbfp3Ws6CxdP9w_pZBHmPI7akK4FRjwzFLUGL0pof8CxSTKacy0Y49QIwHESAWa5UJhwo9e-NLCcCzYil71vY-uvTrtWburO-jedpIARYxihh656KLe1c1Yb2dhiq-xOIsjfgKQPSPYBefhi79hlW73-R_eJeCDsgcK1-vvvruynHMcsjuTsfSXFav6YzpdcPrMfq0Jvzw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>201533151</pqid></control><display><type>article</type><title>Functional Analysis of Water Channels in Barley Roots</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Katsuhara, Maki ; Akiyama, Yoshiko ; Koshio, Kazuki ; Shibasaka, Mineo ; Kasamo, Kunihiro</creator><creatorcontrib>Katsuhara, Maki ; Akiyama, Yoshiko ; Koshio, Kazuki ; Shibasaka, Mineo ; Kasamo, Kunihiro</creatorcontrib><description>We identified three genes homologous to water channels in the plasma membrane type subfamily from roots of barley seedlings. These genes were designated HvPIP2;1, HvPIP1;3, and HvPIP1;5 after comparison to Arabidopsis aquaporins. Competitive reverse transcription (RT)-PCR was applied in order to distinguish and to quantify their transcripts. The HvPIP2;1 transcript was the most abundant among the three in roots. Salt stress (200 mM NaCl) down-regulated HvPIP2;1 (transcript and protein), but had almost no effect on the expressions of HvPIP1;3, or HvPIP1;5. Approximately equal amounts of the transcripts of the three were detected in shoots, and salt stress enhanced the expression of HvPIP2;1 but not of HvPIP1;3, or HvPIP1;5. HvPIP2;1 protein was confirmed to be localized in the plasma membrane. Functional expression of HvPIP2;1 in Xenopus oocytes confirmed that HvPIP2;1 encoded an aquaporin that transports water. This water permeability was reduced by HgCl2, which is a typical water channel inhibitor. This activity was not modified by some inhibitors against protein kinase and protein phosphatase.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/pcf102</identifier><identifier>PMID: 12198191</identifier><language>eng</language><publisher>Japan: Oxford University Press</publisher><subject>3-isobutyle-1-methylxanthine ; Adaptation, Physiological - genetics ; Adaptation, Physiological - physiology ; Algorithms ; Amino Acid Sequence ; Animals ; Aquaporins - antagonists & inhibitors ; Aquaporins - genetics ; Aquaporins - physiology ; Arabidopsis Proteins ; Carbazoles - pharmacology ; Cell Membrane - metabolism ; Cell Membrane Permeability - drug effects ; dimethylsulfoxide ; dithiothreitol ; DMSO ; DNA, Complementary - genetics ; DNA, Complementary - metabolism ; DTT ; Female ; Gene Expression Regulation, Plant ; Hordeum - drug effects ; Hordeum - genetics ; Hordeum - physiology ; IBMX ; Indole Alkaloids ; Ion Channels - genetics ; Keywords: Aquaporin — Barley — Competitive RT-PCR — Salt stress ; MBS ; Mercuric Chloride - pharmacology ; modified Barth’s solution ; Molecular Sequence Data ; N′-bis(2-ethanesulfonic acid) ; Okadaic Acid - pharmacology ; Oocytes - physiology ; Osmotic Pressure - drug effects ; Osmotic water permeability ; phenylmethylsulfonylfluoride ; Phosphoprotein Phosphatases - antagonists & inhibitors ; Phosphorylation ; Phylogeny ; PIP ; piperazine-N ; PIPES ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Roots - drug effects ; Plant Roots - genetics ; Plant Roots - physiology ; Plasma membrane Intrinsic Protein ; PMSF ; Protein Kinase Inhibitors ; RACE ; rapid amplification of cDNA end ; reverse transcription-PCR ; RT-PCR ; Sequence Homology, Amino Acid ; Sodium Chloride - pharmacology ; TIP ; Tonoplast Intrinsic Protein ; Water - pharmacology ; Water - physiology ; Xenopus laevis</subject><ispartof>Plant and cell physiology, 2002-08, Vol.43 (8), p.885-893</ispartof><rights>Copyright Oxford University Press(England) Aug 15, 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-2d9154bf21ee0bf2a9ec4716f8b2c4e93342f90168501bc9a184fededee03c493</citedby><cites>FETCH-LOGICAL-c475t-2d9154bf21ee0bf2a9ec4716f8b2c4e93342f90168501bc9a184fededee03c493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12198191$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Katsuhara, Maki</creatorcontrib><creatorcontrib>Akiyama, Yoshiko</creatorcontrib><creatorcontrib>Koshio, Kazuki</creatorcontrib><creatorcontrib>Shibasaka, Mineo</creatorcontrib><creatorcontrib>Kasamo, Kunihiro</creatorcontrib><title>Functional Analysis of Water Channels in Barley Roots</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>We identified three genes homologous to water channels in the plasma membrane type subfamily from roots of barley seedlings. These genes were designated HvPIP2;1, HvPIP1;3, and HvPIP1;5 after comparison to Arabidopsis aquaporins. Competitive reverse transcription (RT)-PCR was applied in order to distinguish and to quantify their transcripts. The HvPIP2;1 transcript was the most abundant among the three in roots. Salt stress (200 mM NaCl) down-regulated HvPIP2;1 (transcript and protein), but had almost no effect on the expressions of HvPIP1;3, or HvPIP1;5. Approximately equal amounts of the transcripts of the three were detected in shoots, and salt stress enhanced the expression of HvPIP2;1 but not of HvPIP1;3, or HvPIP1;5. HvPIP2;1 protein was confirmed to be localized in the plasma membrane. Functional expression of HvPIP2;1 in Xenopus oocytes confirmed that HvPIP2;1 encoded an aquaporin that transports water. This water permeability was reduced by HgCl2, which is a typical water channel inhibitor. This activity was not modified by some inhibitors against protein kinase and protein phosphatase.</description><subject>3-isobutyle-1-methylxanthine</subject><subject>Adaptation, Physiological - genetics</subject><subject>Adaptation, Physiological - physiology</subject><subject>Algorithms</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Aquaporins - antagonists & inhibitors</subject><subject>Aquaporins - genetics</subject><subject>Aquaporins - physiology</subject><subject>Arabidopsis Proteins</subject><subject>Carbazoles - pharmacology</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>dimethylsulfoxide</subject><subject>dithiothreitol</subject><subject>DMSO</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Complementary - metabolism</subject><subject>DTT</subject><subject>Female</subject><subject>Gene Expression Regulation, Plant</subject><subject>Hordeum - drug effects</subject><subject>Hordeum - genetics</subject><subject>Hordeum - physiology</subject><subject>IBMX</subject><subject>Indole Alkaloids</subject><subject>Ion Channels - genetics</subject><subject>Keywords: Aquaporin — Barley — Competitive RT-PCR — Salt stress</subject><subject>MBS</subject><subject>Mercuric Chloride - pharmacology</subject><subject>modified Barth’s solution</subject><subject>Molecular Sequence Data</subject><subject>N′-bis(2-ethanesulfonic acid)</subject><subject>Okadaic Acid - pharmacology</subject><subject>Oocytes - physiology</subject><subject>Osmotic Pressure - drug effects</subject><subject>Osmotic water permeability</subject><subject>phenylmethylsulfonylfluoride</subject><subject>Phosphoprotein Phosphatases - antagonists & inhibitors</subject><subject>Phosphorylation</subject><subject>Phylogeny</subject><subject>PIP</subject><subject>piperazine-N</subject><subject>PIPES</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Roots - drug effects</subject><subject>Plant Roots - genetics</subject><subject>Plant Roots - physiology</subject><subject>Plasma membrane Intrinsic Protein</subject><subject>PMSF</subject><subject>Protein Kinase Inhibitors</subject><subject>RACE</subject><subject>rapid amplification of cDNA end</subject><subject>reverse transcription-PCR</subject><subject>RT-PCR</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sodium Chloride - pharmacology</subject><subject>TIP</subject><subject>Tonoplast Intrinsic Protein</subject><subject>Water - pharmacology</subject><subject>Water - physiology</subject><subject>Xenopus laevis</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkNFLwzAQxoMobk5f_AOk-ChU75J0bR5ncc4xUUSZ7CWkXYKdXVuTFtx_b6RDOe6-g_vxcXyEnCNcIwh20-SNb4NAD8gQeYyhgIgdkiEAoyHECQ7IiXMbAL8zOCYDpCgSFDgk0bSr8raoK1UGEz92rnBBbYKlarUN0g9VVbp0QVEFt8qWehe81HXrTsmRUaXTZ3sdkbfp3Ws6CxdP9w_pZBHmPI7akK4FRjwzFLUGL0pof8CxSTKacy0Y49QIwHESAWa5UJhwo9e-NLCcCzYil71vY-uvTrtWburO-jedpIARYxihh656KLe1c1Yb2dhiq-xOIsjfgKQPSPYBefhi79hlW73-R_eJeCDsgcK1-vvvruynHMcsjuTsfSXFav6YzpdcPrMfq0Jvzw</recordid><startdate>20020815</startdate><enddate>20020815</enddate><creator>Katsuhara, Maki</creator><creator>Akiyama, Yoshiko</creator><creator>Koshio, Kazuki</creator><creator>Shibasaka, Mineo</creator><creator>Kasamo, Kunihiro</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20020815</creationdate><title>Functional Analysis of Water Channels in Barley Roots</title><author>Katsuhara, Maki ; Akiyama, Yoshiko ; Koshio, Kazuki ; Shibasaka, Mineo ; Kasamo, Kunihiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-2d9154bf21ee0bf2a9ec4716f8b2c4e93342f90168501bc9a184fededee03c493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>3-isobutyle-1-methylxanthine</topic><topic>Adaptation, Physiological - genetics</topic><topic>Adaptation, Physiological - physiology</topic><topic>Algorithms</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Aquaporins - antagonists & inhibitors</topic><topic>Aquaporins - genetics</topic><topic>Aquaporins - physiology</topic><topic>Arabidopsis Proteins</topic><topic>Carbazoles - pharmacology</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>dimethylsulfoxide</topic><topic>dithiothreitol</topic><topic>DMSO</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Complementary - metabolism</topic><topic>DTT</topic><topic>Female</topic><topic>Gene Expression Regulation, Plant</topic><topic>Hordeum - drug effects</topic><topic>Hordeum - genetics</topic><topic>Hordeum - physiology</topic><topic>IBMX</topic><topic>Indole Alkaloids</topic><topic>Ion Channels - genetics</topic><topic>Keywords: Aquaporin — Barley — Competitive RT-PCR — Salt stress</topic><topic>MBS</topic><topic>Mercuric Chloride - pharmacology</topic><topic>modified Barth’s solution</topic><topic>Molecular Sequence Data</topic><topic>N′-bis(2-ethanesulfonic acid)</topic><topic>Okadaic Acid - pharmacology</topic><topic>Oocytes - physiology</topic><topic>Osmotic Pressure - drug effects</topic><topic>Osmotic water permeability</topic><topic>phenylmethylsulfonylfluoride</topic><topic>Phosphoprotein Phosphatases - antagonists & inhibitors</topic><topic>Phosphorylation</topic><topic>Phylogeny</topic><topic>PIP</topic><topic>piperazine-N</topic><topic>PIPES</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Roots - drug effects</topic><topic>Plant Roots - genetics</topic><topic>Plant Roots - physiology</topic><topic>Plasma membrane Intrinsic Protein</topic><topic>PMSF</topic><topic>Protein Kinase Inhibitors</topic><topic>RACE</topic><topic>rapid amplification of cDNA end</topic><topic>reverse transcription-PCR</topic><topic>RT-PCR</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sodium Chloride - pharmacology</topic><topic>TIP</topic><topic>Tonoplast Intrinsic Protein</topic><topic>Water - pharmacology</topic><topic>Water - physiology</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Katsuhara, Maki</creatorcontrib><creatorcontrib>Akiyama, Yoshiko</creatorcontrib><creatorcontrib>Koshio, Kazuki</creatorcontrib><creatorcontrib>Shibasaka, Mineo</creatorcontrib><creatorcontrib>Kasamo, Kunihiro</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant and cell physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Katsuhara, Maki</au><au>Akiyama, Yoshiko</au><au>Koshio, Kazuki</au><au>Shibasaka, Mineo</au><au>Kasamo, Kunihiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional Analysis of Water Channels in Barley Roots</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2002-08-15</date><risdate>2002</risdate><volume>43</volume><issue>8</issue><spage>885</spage><epage>893</epage><pages>885-893</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>We identified three genes homologous to water channels in the plasma membrane type subfamily from roots of barley seedlings. These genes were designated HvPIP2;1, HvPIP1;3, and HvPIP1;5 after comparison to Arabidopsis aquaporins. Competitive reverse transcription (RT)-PCR was applied in order to distinguish and to quantify their transcripts. The HvPIP2;1 transcript was the most abundant among the three in roots. Salt stress (200 mM NaCl) down-regulated HvPIP2;1 (transcript and protein), but had almost no effect on the expressions of HvPIP1;3, or HvPIP1;5. Approximately equal amounts of the transcripts of the three were detected in shoots, and salt stress enhanced the expression of HvPIP2;1 but not of HvPIP1;3, or HvPIP1;5. HvPIP2;1 protein was confirmed to be localized in the plasma membrane. Functional expression of HvPIP2;1 in Xenopus oocytes confirmed that HvPIP2;1 encoded an aquaporin that transports water. This water permeability was reduced by HgCl2, which is a typical water channel inhibitor. This activity was not modified by some inhibitors against protein kinase and protein phosphatase.</abstract><cop>Japan</cop><pub>Oxford University Press</pub><pmid>12198191</pmid><doi>10.1093/pcp/pcf102</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0781 |
| ispartof | Plant and cell physiology, 2002-08, Vol.43 (8), p.885-893 |
| issn | 0032-0781 1471-9053 |
| language | eng |
| recordid | cdi_proquest_journals_201533151 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | 3-isobutyle-1-methylxanthine Adaptation, Physiological - genetics Adaptation, Physiological - physiology Algorithms Amino Acid Sequence Animals Aquaporins - antagonists & inhibitors Aquaporins - genetics Aquaporins - physiology Arabidopsis Proteins Carbazoles - pharmacology Cell Membrane - metabolism Cell Membrane Permeability - drug effects dimethylsulfoxide dithiothreitol DMSO DNA, Complementary - genetics DNA, Complementary - metabolism DTT Female Gene Expression Regulation, Plant Hordeum - drug effects Hordeum - genetics Hordeum - physiology IBMX Indole Alkaloids Ion Channels - genetics Keywords: Aquaporin — Barley — Competitive RT-PCR — Salt stress MBS Mercuric Chloride - pharmacology modified Barth’s solution Molecular Sequence Data N′-bis(2-ethanesulfonic acid) Okadaic Acid - pharmacology Oocytes - physiology Osmotic Pressure - drug effects Osmotic water permeability phenylmethylsulfonylfluoride Phosphoprotein Phosphatases - antagonists & inhibitors Phosphorylation Phylogeny PIP piperazine-N PIPES Plant Proteins - genetics Plant Proteins - metabolism Plant Roots - drug effects Plant Roots - genetics Plant Roots - physiology Plasma membrane Intrinsic Protein PMSF Protein Kinase Inhibitors RACE rapid amplification of cDNA end reverse transcription-PCR RT-PCR Sequence Homology, Amino Acid Sodium Chloride - pharmacology TIP Tonoplast Intrinsic Protein Water - pharmacology Water - physiology Xenopus laevis |
| title | Functional Analysis of Water Channels in Barley Roots |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T22%3A20%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Functional%20Analysis%20of%20Water%20Channels%20in%20Barley%20Roots&rft.jtitle=Plant%20and%20cell%20physiology&rft.au=Katsuhara,%20Maki&rft.date=2002-08-15&rft.volume=43&rft.issue=8&rft.spage=885&rft.epage=893&rft.pages=885-893&rft.issn=0032-0781&rft.eissn=1471-9053&rft_id=info:doi/10.1093/pcp/pcf102&rft_dat=%3Cproquest_cross%3E325279751%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=201533151&rft_id=info:pmid/12198191&rfr_iscdi=true |