Trans-Spliced Leader Addition to mRNAs in a Cnidarian
A search of databases with the sequence from the 5′ untranslated region of a Hydra cDNA clone encoding a receptor protein-tyrosine kinase revealed that a number of Hydra cDNAs contain one of two different sequences at their 5′ ends. This finding suggested the possibility that mRNAs in Hydra receive...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2001-05, Vol.98 (10), p.5693-5698 |
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| creator | Stover, Nicholas A. Steele, Robert E. |
| description | A search of databases with the sequence from the 5′ untranslated region of a Hydra cDNA clone encoding a receptor protein-tyrosine kinase revealed that a number of Hydra cDNAs contain one of two different sequences at their 5′ ends. This finding suggested the possibility that mRNAs in Hydra receive leader sequences by trans-splicing. This hypothesis was confirmed by the finding that the leader sequences are transcribed as parts of small RNAs encoded by genes located in the 5S rRNA clusters of Hydra. The two spliced leader (SL) RNAs (SL-A and -B) contain splice donor dinucleotides at the predicted positions, and genes that receive SLs contain splice acceptor dinucleotides at the predicted positions. Both of the SL RNAs are bound by antibody against trimethyl-guanosine, suggesting that they contain a trimethylguanosine cap. The predicted secondary structures of the Hydra SL RNAs show significant differences from the structures predicted for the SLs of other organisms. Messenger RNAs have been identified that can receive either SL-A or -B, although the impact of the two different SLs on the function of the mRNA is unknown. The presence and features of SL addition in the phylum Cnidaria raise interesting questions regarding the evolution of this process. |
| doi_str_mv | 10.1073/pnas.101049998 |
| format | Article |
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This finding suggested the possibility that mRNAs in Hydra receive leader sequences by trans-splicing. This hypothesis was confirmed by the finding that the leader sequences are transcribed as parts of small RNAs encoded by genes located in the 5S rRNA clusters of Hydra. The two spliced leader (SL) RNAs (SL-A and -B) contain splice donor dinucleotides at the predicted positions, and genes that receive SLs contain splice acceptor dinucleotides at the predicted positions. Both of the SL RNAs are bound by antibody against trimethyl-guanosine, suggesting that they contain a trimethylguanosine cap. The predicted secondary structures of the Hydra SL RNAs show significant differences from the structures predicted for the SLs of other organisms. Messenger RNAs have been identified that can receive either SL-A or -B, although the impact of the two different SLs on the function of the mRNA is unknown. The presence and features of SL addition in the phylum Cnidaria raise interesting questions regarding the evolution of this process.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.101049998</identifier><identifier>PMID: 11331766</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Animals ; Base Sequence ; Biological Sciences ; Cnidaria ; Complementary DNA ; DNA ; Evolution ; Exons ; Genes ; Hydra ; Hydra - genetics ; leader sequence ; Messenger RNA ; Molecular Sequence Data ; Proteins ; Ribonucleic acid ; RNA ; RNA Splicing ; RNA, Messenger - genetics ; RNA, Ribosomal, 5S - genetics ; rRNA genes ; Sequence Homology, Nucleic Acid ; Spliced leader RNA ; Stover ; trimethylguanosine</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2001-05, Vol.98 (10), p.5693-5698</ispartof><rights>Copyright 1993-2001 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences May 8, 2001</rights><rights>Copyright © 2001, The National Academy of Sciences 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c584t-a77bb4fae94527f2eb46cd130aa3bb907bd7912650037d40997e0a796985f84e3</citedby><cites>FETCH-LOGICAL-c584t-a77bb4fae94527f2eb46cd130aa3bb907bd7912650037d40997e0a796985f84e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/98/10.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3055689$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3055689$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27923,27924,53790,53792,58016,58249</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11331766$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stover, Nicholas A.</creatorcontrib><creatorcontrib>Steele, Robert E.</creatorcontrib><title>Trans-Spliced Leader Addition to mRNAs in a Cnidarian</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A search of databases with the sequence from the 5′ untranslated region of a Hydra cDNA clone encoding a receptor protein-tyrosine kinase revealed that a number of Hydra cDNAs contain one of two different sequences at their 5′ ends. 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The presence and features of SL addition in the phylum Cnidaria raise interesting questions regarding the evolution of this process.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological Sciences</subject><subject>Cnidaria</subject><subject>Complementary DNA</subject><subject>DNA</subject><subject>Evolution</subject><subject>Exons</subject><subject>Genes</subject><subject>Hydra</subject><subject>Hydra - genetics</subject><subject>leader sequence</subject><subject>Messenger RNA</subject><subject>Molecular Sequence Data</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Splicing</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Ribosomal, 5S - genetics</subject><subject>rRNA genes</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Spliced leader RNA</subject><subject>Stover</subject><subject>trimethylguanosine</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUha2qiA6FbVeoiljAKuU6fktsRiNe0qiVoKwtJ3bAo4w9tRME_x5HMwyFRVndK93vHB37IHSB4QqDIK93weSyYaBKKXmCFhgUrjlVcIoWAI2oJW3oGXqS8wYAFJPwGJ1hTAgWnC8Qu00m5PrzbvCds9XaGetStbTWjz6GaozV9tP1Mlc-VKZaBW9N8iY8RY96M2T37DDP0Zd3b29XH-r1zfuPq-W67pikY22EaFvaG6coa0TfuJbyzmICxpC2VSBaKxRuOAMgwlJQSjgwQnElWS-pI-fozd53N7VbZzsXxmQGvUt-a9JPHY3Xf1-C_6a_xu-akEawIn95kKd4N7k86q3PnRsGE1ycshYgKTDO_wtiCQJAkgK--AfcxCmF8ge6AVzMGMUFutpDXYo5J9cfA2PQc2t6bk0fWyuCy_vP_IMfaroHzMLfZyVnQ8bVnOvVg4Dup2EY3Y-xkM_35CaPMR1RUqJzqcgvhDeyYA</recordid><startdate>20010508</startdate><enddate>20010508</enddate><creator>Stover, Nicholas A.</creator><creator>Steele, Robert E.</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><general>The National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20010508</creationdate><title>Trans-Spliced Leader Addition to mRNAs in a Cnidarian</title><author>Stover, Nicholas A. ; 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| subjects | Animals Base Sequence Biological Sciences Cnidaria Complementary DNA DNA Evolution Exons Genes Hydra Hydra - genetics leader sequence Messenger RNA Molecular Sequence Data Proteins Ribonucleic acid RNA RNA Splicing RNA, Messenger - genetics RNA, Ribosomal, 5S - genetics rRNA genes Sequence Homology, Nucleic Acid Spliced leader RNA Stover trimethylguanosine |
| title | Trans-Spliced Leader Addition to mRNAs in a Cnidarian |
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