Characterization of the 1918 "Spanish" Influenza Virus Neuraminidase Gene

The "Spanish" influenza pandemic of 1918 was characterized by exceptionally high mortality, especially among young adults. The surface proteins of influenza viruses, hemagglutinin and neuraminidase, play important roles in virulence, host specificity, and the human immune response. The com...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 2000-06, Vol.97 (12), p.6785-6790
Hauptverfasser: Reid, Ann H., Fanning, Thomas G., Janczewski, Thomas A., Taubenberger, Jeffery K.
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container_issue 12
container_start_page 6785
container_title Proceedings of the National Academy of Sciences - PNAS
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creator Reid, Ann H.
Fanning, Thomas G.
Janczewski, Thomas A.
Taubenberger, Jeffery K.
description The "Spanish" influenza pandemic of 1918 was characterized by exceptionally high mortality, especially among young adults. The surface proteins of influenza viruses, hemagglutinin and neuraminidase, play important roles in virulence, host specificity, and the human immune response. The complete coding sequence of hemagglutinin was reported last year. This laboratory has now determined the complete coding sequence of the neuraminidase gene of the 1918 virus. Influenza RNA fragments were isolated from lung tissue of three victims of the 1918 flu; complete sequence was generated from A/Brevig Mission/1/18, with confirmatory sequencing carried out on A/South Carolina/1/18 and A/New York/1/18. The 1918 neuraminidase gene sequence was compared with other N1 subtype neuraminidase genes, including 9 N1 strains newly sequenced for this study. The 1918 neuraminidase shares many sequence and structural characteristics with avian strains, including the conserved active site, wild-type stalk length, glycosylation sites, and antigenic sites. Phylogenetically, the 1918 neuraminidase gene appears to be intermediate between mammals and birds, suggesting that it was introduced into mammals just before the 1918 pandemic.
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subjects Active sites
Adult
Amino Acid Sequence
Amino acids
Base Sequence
Biological Sciences
Catalytic Domain
Epidemics
Female
Genes
Glycosylation
H1N1 subtype influenza A virus
History
Humans
Influenza
Influenza A virus - classification
Influenza A virus - enzymology
Influenza A virus - genetics
Influenza virus
Male
Microbiology
Molecular Sequence Data
Neuraminidase - chemistry
Neuraminidase - genetics
Nucleotides
Pandemics
Pathology
Phylogenetics
Phylogeny
Spain
Swine
Virulence
Viruses
title Characterization of the 1918 "Spanish" Influenza Virus Neuraminidase Gene
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