Structure of Trichodiene Synthase from Fusarium sporotrichioides Provides Mechanistic Inferences on the Terpene Cyclization Cascade

The x-ray crystal structure of recombinant trichodiene synthase from Fusarium sporotrichioides has been determined to 2.5-Å resolution, both unliganded and complexed with inorganic pyrophosphate. This reaction product coordinates to three Mg2+ions near the mouth of the active site cleft. A compariso...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2001-11, Vol.98 (24), p.13543-13548
Hauptverfasser:	Rynkiewicz, Michael J., Cane, David E., Christianson, David W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Active sites Amino Acid Sequence Atoms Biological Sciences Biosynthesis Carbon-Carbon Lyases - chemistry Carbon-Carbon Lyases - metabolism Coordinate systems Crystallography Crystallography, X-Ray Diphosphates Diphosphates - chemistry Diphosphates - metabolism Enzymes Fusarium - enzymology Fusarium sporotrichioides Ligands Models, Molecular Molecular Sequence Data Monomers Polymers Protein Structure, Tertiary Sesquiterpenes Terpenes Terpenes - metabolism Terpenoids
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